Disease Information

General Information of the Disease (ID: DIS00100)
Name
Retina cancer
ICD
ICD-11: 2D02
Resistance Map
Type(s) of Resistant Mechanism of This Disease
  EADR: Epigenetic Alteration of DNA, RNA or Protein
  IDUE: Irregularity in Drug Uptake and Drug Efflux
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Drug
Approved Drug(s)
7 drug(s) in total
Click to Show/Hide the Full List of Drugs
Etoposide
Click to Show/Hide
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Etoposide
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Differential expression of the molecule in resistant disease
Classification of Disease Retina cancer [ICD-11: 2D02]
The Specified Disease Retinoblastoma tumor
The Studied Tissue Uvea
The Expression Level of Disease Section Compare with the Healthy Individual Tissue
p-value: 1.43E-02
Fold-change: -1.35E-01
Z-score: -2.66E+00
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-mir-34 [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Etoposide
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Key Molecule: hsa-miR-3163 [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Etoposide
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 RT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: Melanoma antigen A 4 (MAGE4) [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Etoposide
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 Western blot analysis; RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-miR-34a [2]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Etoposide
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation MAGE-A/p53 signaling Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
Y79 cells Retina Homo sapiens (Human) CVCL_1893
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 Reverse transcription-quantitative polymerase chain reaction
Experiment for
Drug Resistance		 MTT assay
Mechanism Description The results indicated that SO-Rb50 cells exhibited the highest resistance to carboplatin, Adriamycin and vincristine (P<0.05), whereas HXO-Rb44 cells revealed the highest inhibition rate in response to etoposide (P<0.05) out of the four cell lines. Furthermore, reduced miR-34a expression and increased MAGE-A expression significantly elevated the survival rate and viability of SO-Rb50 cells following drug treatment (all P<0.05). miR-34a was also demonstrated to directly target MAGE-A, thereby significantly promoting the viability of RB cells and depressing apoptosis (P<0.05). p53, which was subjected to modulation by miR-34a and MAGE-A, also significantly reduced the proliferation rate of RB cells (P<0.05). In conclusion, the miR-34a/MAGE-A/p53 axis may be conducive to enhancing the efficacies of chemotherapeutic treatments for RB.
Carboplatin
Click to Show/Hide
Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-miR-34a [2]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Carboplatin
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation MAGE-A/p53 signaling Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
Y79 cells Retina Homo sapiens (Human) CVCL_1893
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 Reverse transcription-quantitative polymerase chain reaction
Experiment for
Drug Resistance		 MTT assay
Mechanism Description The results indicated that SO-Rb50 cells exhibited the highest resistance to carboplatin, Adriamycin and vincristine (P<0.05), whereas HXO-Rb44 cells revealed the highest inhibition rate in response to etoposide (P<0.05) out of the four cell lines. Furthermore, reduced miR-34a expression and increased MAGE-A expression significantly elevated the survival rate and viability of SO-Rb50 cells following drug treatment (all P<0.05). miR-34a was also demonstrated to directly target MAGE-A, thereby significantly promoting the viability of RB cells and depressing apoptosis (P<0.05). p53, which was subjected to modulation by miR-34a and MAGE-A, also significantly reduced the proliferation rate of RB cells (P<0.05). In conclusion, the miR-34a/MAGE-A/p53 axis may be conducive to enhancing the efficacies of chemotherapeutic treatments for RB.
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-mir-34 [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Carboplatin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Key Molecule: hsa-miR-3163 [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Carboplatin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 RT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Carboplatin
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: Melanoma antigen A 4 (MAGE4) [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Carboplatin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 Western blot analysis; RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Cisplatin
Click to Show/Hide
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-miR-3163 [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Cisplatin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 RT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Cisplatin
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
Doxorubicin
Click to Show/Hide
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-mir-34 [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Doxorubicin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Key Molecule: hsa-miR-3163 [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Doxorubicin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 RT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Doxorubicin
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: Melanoma antigen A 4 (MAGE4) [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Doxorubicin
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 Western blot analysis; RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Melphalan
Click to Show/Hide
Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Melphalan
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Key Molecule: Multidrug resistance-associated protein 1 (MRP1) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Melphalan
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Key Molecule: Multidrug resistance protein 1 (ABCB1) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Melphalan
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Topotecan
Click to Show/Hide
Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Topotecan
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Key Molecule: Multidrug resistance protein 1 (ABCB1) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Topotecan
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Key Molecule: L-type amino acid transporter 1 (LAT1/SLC7A5) [4]
Resistant Disease Retinoblastoma [ICD-11: 2D02.2]
Resistant Drug Topotecan
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Discovered Using In-vivo Testing Model
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 MTT assay
Mechanism Description Similar Genomic Alterations but Distinctive Expression of Influx/Efflux Transporters Between Chemoresistant and Parental Cells
Vincristine
Click to Show/Hide
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: hsa-mir-34 [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Vincristine
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
Key Molecule: hsa-miR-3163 [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Vincristine
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Experiment for
Molecule Alteration		 RT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Irregularity in Drug Uptake and Drug Efflux (IDUE) Click to Show/Hide
Key Molecule: ATP-binding cassette sub-family G2 (ABCG2) [1]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Vincristine
 Drug Info 
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
In Vitro Model WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Silencing of ABCG2 by MicroRNA-3163 inhibits multidrug resistance in retinoblastoma cancer stem cells.
  Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: Melanoma antigen A 4 (MAGE4) [3]
Sensitive Disease Retinoblastoma [ICD-11: 2D02.2]
Sensitive Drug Vincristine
 Drug Info 
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
MAGE-A/p53 signaling pathway Regulation N.A.
In Vitro Model HXO-Rb44 cells Retina Homo sapiens (Human) CVCL_D542
SO-Rb50 cells Retina Homo sapiens (Human) CVCL_D543
WERI-Rb-1 cells Retina Homo sapiens (Human) CVCL_1792
Y79 cells Retina Homo sapiens (Human) CVCL_1893
Experiment for
Molecule Alteration		 Western blot analysis; RT-qPCR
Experiment for
Drug Resistance		 Freedom Evolyzer-2200 Enzyme-Linked Immunometric meter; Flow cytometry assay
Mechanism Description miR-34a may function as a tumor suppressor for RB by targeting MAGE-A and upregulating p53 expression to enhance cell apoptosis and chemosensitivity (Carboplatin; Etoposide; Adriamycin; vincristine).
References
Ref 1 Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells. J Korean Med Sci. 2016 Jun;31(6):836-42. doi: 10.3346/jkms.2016.31.6.836. Epub 2016 Apr 20.
Ref 2 Molecular Basis for Necitumumab Inhibition of EGFR Variants Associated with Acquired Cetuximab ResistanceMol Cancer Ther. 2018 Feb;17(2):521-531. doi: 10.1158/1535-7163.MCT-17-0575. Epub 2017 Nov 20.
Ref 3 miR 34a regulates the chemosensitivity of retinoblastoma cells via modulation of MAGE A/p53 signaling. Int J Oncol. 2019 Jan;54(1):177-187. doi: 10.3892/ijo.2018.4613. Epub 2018 Oct 31.
Ref 4 Mimicking Retinoblastoma Treatment With Repeated Topotecan or Melphalan Develops Cross-Resistance to Classic Agents But Not to Repurposed Drugs. Invest Ophthalmol Vis Sci. 2024 Dec 2;65(14):14.

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