Molecule Information

General Information of the Molecule (ID: Mol00075)

Name	L-glutamine amidohydrolase (GLS) ,Homo sapiens
Synonyms	GLS; K-glutaminase; L-glutamine amidohydrolase; GLS1; KIAA0838 Click to Show/Hide
Molecule Type	Protein
Gene Name	GLS
Gene ID	2744
Location	chr2:190880821-190965552[+]
Sequence	MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGG GGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEG KELVASGENKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMD MLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKK QSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPLKYAIAVNDL GTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQ FLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSI EVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPA KSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKL DPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVE VVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQT VHKNLDGLL Click to Show/Hide
Function	Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. Click to Show/Hide
Uniprot ID	GLSK_HUMAN
Ensembl ID	ENSG00000115419
HGNC ID	HGNC:4331
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Temozolomide

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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)			Click to Show/Hide
Disease Class: Melanoma				[1]
Sensitive Disease	Melanoma [ICD-11: 2C30.0]			Disease Info
Sensitive Drug	Temozolomide			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Revealed Based on the Cell Line Data
In Vitro Model	HT144 cells	Skin	Homo sapiens (Human)	CVCL_0318
	SkMEL5 cells	Skin	Homo sapiens (Human)	CVCL_0527
Experiment for Molecule Alteration	Dual luciferase reporter assay; Western blot analysis; Immunohistochemistry assays
Experiment for Drug Resistance	MTT assay
Mechanism Description	Overexpression of miR203 sensitizes MM cells to TMZ by targeting GLS.

Disease- and Tissue-specific Abundances of This Molecule

ICD Disease Classification 02

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Melanoma [ICD-11: 2C30]

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Differential expression of molecule in resistant diseases
The Studied Tissue	Skin
The Specified Disease	Melanoma
The Expression Level of Disease Section Compare with the Healthy Individual Tissue	p-value: 5.27E-01; Fold-change: 1.54E-01; Z-score: 1.07E-01
Molecule expression in the diseased tissue of patients Molecule expression in the normal tissue of healthy individuals
Disease-specific Molecule Abundances		Click to View the Clearer Original Diagram
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Tissue-specific Molecule Abundances in Healthy Individuals

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Download the Tissue-Specific Variation(s) Profile

References

Ref 1	Sensitization of melanoma cells to temozolomide by overexpression of microRNA 203 through direct targeting of glutaminase-mediated glutamine metabolism. Clin Exp Dermatol. 2017 Aug;42(6):614-621. doi: 10.1111/ced.13119. Epub 2017 Jun 9.