Molecule Information

General Information of the Molecule (ID: Mol00832)

Name	Beta-lactamase (BLA) ,Citrobacter freundii
Synonyms	bla CTX-M-3; blaCTX-M-3; blaCTX-M3; AN672_26965 Click to Show/Hide
Molecule Type	Protein
Gene Name	bla
Sequence	MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSGGRLGVALINTADNSQ ILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKKSDLVNYNPIAEKHVNGTM SLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETFRLDRTEPTLNTAIPGDP RDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAASIQAGLPASWVVGDKTGS GDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLASAAKIVTDGL Click to Show/Hide
Uniprot ID	O52904_CITFR
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Citrobacter Species: Citrobacter freundii

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

5 drug(s) in total

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Ampicillin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Ampicillin		Drug Info
Molecule Alteration	Missense mutation	p.V77A+p.D114N+p.S140A+p.N288D
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Citrobacter freundii strain 2524/96		546
	Citrobacter freundii strain 2525/96		546
	Citrobacter freundii strain 2526/96		546
	Escherichia coli strain 2527/96		562
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 Beta-lactamase.

Aztreonam

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Aztreonam		Drug Info
Molecule Alteration	Missense mutation	p.V77A+p.D114N+p.S140A+p.N288D
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Citrobacter freundii strain 2524/96		546
	Citrobacter freundii strain 2525/96		546
	Citrobacter freundii strain 2526/96		546
	Escherichia coli strain 2527/96		562
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 Beta-lactamase.

Cefotaxime

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Cefotaxime		Drug Info
Molecule Alteration	Missense mutation	p.V77A+p.D114N+p.S140A+p.N288D
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Citrobacter freundii strain 2524/96		546
	Citrobacter freundii strain 2525/96		546
	Citrobacter freundii strain 2526/96		546
	Escherichia coli strain 2527/96		562
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 Beta-lactamase.

Cefoxitin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Cefoxitin		Drug Info
Molecule Alteration	Missense mutation	p.V77A+p.D114N+p.S140A+p.N288D
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Citrobacter freundii strain 2524/96		546
	Citrobacter freundii strain 2525/96		546
	Citrobacter freundii strain 2526/96		546
	Escherichia coli strain 2527/96		562
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 Beta-lactamase.

Piperacillin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Piperacillin		Drug Info
Molecule Alteration	Missense mutation	p.V77A+p.D114N+p.S140A+p.N288D
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Citrobacter freundii strain 2524/96		546
	Citrobacter freundii strain 2525/96		546
	Citrobacter freundii strain 2526/96		546
	Escherichia coli strain 2527/96		562
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 Beta-lactamase.

References

Ref 1	Identifying novel Beta-lactamase substrate activity through in silico prediction of antimicrobial resistance. Microb Genom. 2021 Jan;7(1):mgen000500. doi: 10.1099/mgen.0.000500.
Ref 2	Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme. Antimicrob Agents Chemother. 1998 Apr;42(4):827-32. doi: 10.1128/AAC.42.4.827.