Drug Information

Drug (ID: DG00349) and It's Reported Resistant Information
Name
Calycosin
Synonyms
Calycosin; 20575-57-9; 3'-hydroxyformononetin; 7-hydroxy-3-(3-hydroxy-4-methoxyphenyl)chromen-4-one; UNII-09N3E8P7TA; 7-hydroxy-3-(3-hydroxy-4-methoxyphenyl)-4H-chromen-4-one; 3',7-dihydroxy-4'-methoxyisoflavone; 09N3E8P7TA; CHEBI:17793; 4H-1-Benzopyran-4-one, 7-hydroxy-3-(3-hydroxy-4-methoxyphenyl)-; 7-hydroxy-3-(3-hydroxy-4-methoxy-phenyl)chromen-4-one; 7-hydroxy-3-(3-hydroxy-4-methoxyphenyl)-4H-1-benzopyran-4-one; 7,3'-dihydroxy-4'-methoxyisoflavone; HSDB 8109; 3-Hydroxyformononetin; 3'-hydroxy-formononetin; SCHEMBL73013; MLS000876988; CHEMBL241608; MEGxp0_001325; ACon1_000650; DTXSID70174580; HMS2268B05; BCP28682; HY-N0519; ZINC6018563; LMPK12050056; MFCD00210598; s9038; AKOS015896719; 3',7-dihydroxy-4'-methoxy-isoflavone; AC-8043; CS-3715; MCULE-7559555984; NCGC00169494-01; LS-14468; SMR000440659; DB-045290; FT-0630465; N1412; X1103; C01562; 575C579; A814711; Q-100254; Q5024637; BRD-K05039497-001-01-6
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Structure
Drug Resistance Disease(s)
Disease(s) with Resistance Information Discovered by Cell Line Test for This Drug (1 diseases)
Breast cancer [ICD-11: 2C60]
[1]
Click to Show/Hide the Molecular Information and External Link(s) of This Drug
Formula
C16H12O5
IsoSMILES
COC1=C(C=C(C=C1)C2=COC3=C(C2=O)C=CC(=C3)O)O
InChI
1S/C16H12O5/c1-20-14-5-2-9(6-13(14)18)12-8-21-15-7-10(17)3-4-11(15)16(12)19/h2-8,17-18H,1H3
InChIKey
ZZAJQOPSWWVMBI-UHFFFAOYSA-N
PubChem CID
5280448
Type(s) of Resistant Mechanism of This Drug
  EADR: Epigenetic Alteration of DNA, RNA or Protein
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Their Corresponding Diseases
ICD-02: Benign/in-situ/malignant neoplasm
Click to Show/Hide the Resistance Disease of This Class
Nasopharyngeal cancer [ICD-11: 2B6B]
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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
       Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: Ewing sarcoma associated transcript 1 (EWSAT1) [2]
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Sensitive Disease Nasopharyngeal carcinoma [ICD-11: 2B6B.0]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell proliferation Inhibition hsa05200
Cell viability Inhibition hsa05200
TRAF6-related signaling pathway Regulation hsa05206
In Vitro Model CNE2 cells Nasopharynx Homo sapiens (Human) CVCL_6889
C666-1 cells Throat Homo sapiens (Human) CVCL_7949
CNE1 cells Throat Homo sapiens (Human) CVCL_6888
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 CCK8 assay; BrdU assay
Mechanism Description Calycosin inhibits nasopharyngeal carcinoma cells by downregulating EWSAT1 expression to regulate the TRAF6-related pathways.
       Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: NF-kappa-B inhibitor alpha (NFKBIA) [2]
Molecule Alteration Phosphorylation
Down-regulation
 Molecule Info 
Sensitive Disease Nasopharyngeal carcinoma [ICD-11: 2B6B.0]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell proliferation Inhibition hsa05200
Cell viability Inhibition hsa05200
TRAF6-related signaling pathway Regulation hsa05206
In Vitro Model CNE2 cells Nasopharynx Homo sapiens (Human) CVCL_6889
C666-1 cells Throat Homo sapiens (Human) CVCL_7949
CNE1 cells Throat Homo sapiens (Human) CVCL_6888
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay; BrdU assay
Mechanism Description Calycosin inhibits nasopharyngeal carcinoma cells by downregulating EWSAT1 expression to regulate the TRAF6-related pathways.
Key Molecule: Nuclear receptor subfamily 2 group C2 (NR2C2) [2]
Molecule Alteration Phosphorylation
Down-regulation
 Molecule Info 
Sensitive Disease Nasopharyngeal carcinoma [ICD-11: 2B6B.0]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell proliferation Inhibition hsa05200
Cell viability Inhibition hsa05200
TRAF6-related signaling pathway Regulation hsa05206
In Vitro Model CNE2 cells Nasopharynx Homo sapiens (Human) CVCL_6889
C666-1 cells Throat Homo sapiens (Human) CVCL_7949
CNE1 cells Throat Homo sapiens (Human) CVCL_6888
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay; BrdU assay
Mechanism Description Calycosin inhibits nasopharyngeal carcinoma cells by downregulating EWSAT1 expression to regulate the TRAF6-related pathways.
Key Molecule: Transcription factor Jun (JUN) [2]
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Sensitive Disease Nasopharyngeal carcinoma [ICD-11: 2B6B.0]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell proliferation Inhibition hsa05200
Cell viability Inhibition hsa05200
TRAF6-related signaling pathway Regulation hsa05206
In Vitro Model CNE2 cells Nasopharynx Homo sapiens (Human) CVCL_6889
C666-1 cells Throat Homo sapiens (Human) CVCL_7949
CNE1 cells Throat Homo sapiens (Human) CVCL_6888
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay; BrdU assay
Mechanism Description Calycosin inhibits nasopharyngeal carcinoma cells by downregulating EWSAT1 expression to regulate the TRAF6-related pathways.
Key Molecule: TNF receptor-associated factor 6 (TRAF6) [2]
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Sensitive Disease Nasopharyngeal carcinoma [ICD-11: 2B6B.0]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell proliferation Inhibition hsa05200
Cell viability Inhibition hsa05200
TRAF6-related signaling pathway Regulation hsa05206
In Vitro Model CNE2 cells Nasopharynx Homo sapiens (Human) CVCL_6889
C666-1 cells Throat Homo sapiens (Human) CVCL_7949
CNE1 cells Throat Homo sapiens (Human) CVCL_6888
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay; BrdU assay
Mechanism Description Calycosin inhibits nasopharyngeal carcinoma cells by downregulating EWSAT1 expression to regulate the TRAF6-related pathways.
Breast cancer [ICD-11: 2C60]
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Drug Resistance Data Categorized by Their Corresponding Mechanisms
       Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: WD repeat domain 7 (WDR7) [1]
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Resistant Disease ER negative breast cancer [ICD-11: 2C60.7]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell growth Inhibition hsa05200
WDR7-7/GPR30 signaling pathway Regulation hsa01522
In Vitro Model MCF-7 cells Breast Homo sapiens (Human) CVCL_0031
SkBR3 cells Breast Homo sapiens (Human) CVCL_0033
MDA-MB-231 cells Breast Homo sapiens (Human) CVCL_0062
T47D cells Breast Homo sapiens (Human) CVCL_0553
MDA-MB-468 cells Breast Homo sapiens (Human) CVCL_0419
MCF10A cells Breast Homo sapiens (Human) CVCL_0598
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 qPCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Calycosin inhibited the proliferation of breast cancer cells through WDR7-7-GPR30 signaling. WDR7-7 overexpression led to the inhibition of the GPR30-mediated phosphorylation of SRC, EGFR, ERk1/2, and Akt, ultimately resulting in reduced cell growth, which are key regulators of cell proliferation and survival in breast cancer cells, act as downstream effectors of the WDR7-7-GPR30 pathway.
       Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: G-protein coupled estrogen receptor 1 (GPER1) [1]
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Resistant Disease ER negative breast cancer [ICD-11: 2C60.7]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell growth Inhibition hsa05200
WDR7-7/GPR30 signaling pathway Regulation hsa01522
In Vitro Model MCF-7 cells Breast Homo sapiens (Human) CVCL_0031
SkBR3 cells Breast Homo sapiens (Human) CVCL_0033
MDA-MB-231 cells Breast Homo sapiens (Human) CVCL_0062
T47D cells Breast Homo sapiens (Human) CVCL_0553
MDA-MB-468 cells Breast Homo sapiens (Human) CVCL_0419
MCF10A cells Breast Homo sapiens (Human) CVCL_0598
In Vivo Model Nude mouse xenograft model Mus musculus
Experiment for
Molecule Alteration		 qRT-PCR; Luciferase reporter assay; Western blot analysis
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Calycosin inhibited the proliferation of breast cancer cells through WDR7-7-GPR30 signaling. WDR7-7 overexpression led to the inhibition of the GPR30-mediated phosphorylation of SRC, EGFR, ERk1/2, and Akt, ultimately resulting in reduced cell growth, which are key regulators of cell proliferation and survival in breast cancer cells, act as downstream effectors of the WDR7-7-GPR30 pathway.
References
Ref 1 Calycosin inhibits the in vitro and in vivo growth of breast cancer cells through WDR7-7-GPR30 Signaling. J Exp Clin Cancer Res. 2017 Nov 2;36(1):153. doi: 10.1186/s13046-017-0625-y.
Ref 2 Calycosin inhibits nasopharyngeal carcinoma cells by influencing EWSAT1 expression to regulate the TRAF6-related pathways. Biomed Pharmacother. 2018 Oct;106:342-348. doi: 10.1016/j.biopha.2018.06.143. Epub 2018 Jul 11.

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