Molecule Information

General Information of the Molecule (ID: Mol05828)
Name
FOXD2-AS1 ,Homo sapiens
Molecule Type
Long non-coding RNA
Sequence
CCTGTTCGGCGTCTTGCAGCAGTGCCTGCGATCCCGCGTGAGTCCGTCGCTCCCTCCGCG
AAAACCTGGACTCTTTCCCAGAAGCCTTGGGCTCCAGCACCATGCCTGCTGCAGCCGCCA
AAGAGCGGGAAGCCACAGGCTCTGCGGGTCTTGGTTACAGCGTCTCTGGTCGTGCCGGGC
CTCCTTCTGCATCTGTTCCACGGCCCGGCCATCTCCCTCGTGCAGCCCAGAACAATTGGG
AGGAAGCGTTGGCGGGCGGAGGGGGATGCCTGTTGGGCTTTTCCACTAGTTACTGAGTGT
TTTTTGCGAAGAGTACGTTGCTATCAGTGGCGTCCGGGTCGTCTCTCCCTCTCTTCCTTT
TGGTCCCCATAAATGAGCTGTTCTCGGCTCTGGGAAAGAAAAGCCCTCCAGCGATTATGC
GGATCTAATTAAGTCAATCAGGAAACTGCCGGAGAGCGGCACTGGCTTGAAGCGGAGTTT
GTCCTGAGGCACAGCGGAACGATTGCACGCGGCCCTTTTCAGCGGTTCGAGCTCGGCCAG
CGCCGGGCTGCAAACGGAAGCCTGAGCTGGGCTTGGGGAACTACGCCCGCAGATTTCTCT
AATTTTATTCACAAAACTGGACACCCTCAGGGAAAGCGGCAAAGCCGGCGCGAGCCTCCT
TCCCACTCTGGACCTAGCTGCAGCTCCAAAGCGAGATAGCTGTCTAGCCTCAGCCGGTAC
AGCTCCCGGCCCCTTTCTCCACCCGGATCTTCGGCTGCTCAGAAGCGATCAGCTCCCTTA
GCCCAGGTGGGAGGCGGGAGACTCCAGATCCTGTGACCCGCGGAGGCAGACCTCAGACAT
GTTCCCCATGCACAGAAATGACTGGGGCTCCAGTGTGTGCACCTTCAACTCTGAAGCCCA
CCTGCCCACTTCAGGCGCTGGCGCGGAGATAACCACCACGCGTCTGCCGGGCAAAGTTCG
AGAGTGAATTTACATCTTCTCTTTCCGCTACTCCTGGCCACTGTCCCCTCCCAGCCCCAT
CTCCCCCGCCCCCAACCCTGGACTATGTGGTAGGGGACTCGCTTAAGCACCCAAGTACTG
CGCGGTTGTTGAGACCAAGGCGAGCTGCGGAGCCTGTGGGCTGTTGTTCGTGGGAAGAGG
GTTGCGACCTCAGGAGAGACCGAAAACAGCGCCACTTGAAACCCGCCGGTCCTGAAGATC
ACAGAGTTCCCGGAGTGGTAGGGAAGGCTCCACGCTCTTGAGAGCAGAGGCGGCCAGGGG
CAGTGGGGGACGCTTCCAGGTATGTGGGAAGGAGAACTTCATGTCTAAGTGCGCTCTTGT
GGGTTTCTCGGCCAGCTGGGCCGAGCCTCTCTTTCTCTGTCCTCATGTGTCTGTCCTCTG
GACAACAGTGGCTCTGTATGGCGGATTGCGACCCTGAGTGACGGGCGCCTGTCTTGCATC
CTGTGTCCTGTGTCCTCCCCTCCAGACCTCATGCTTGGTGTCCACAGAGTGGACCCAGGA
CTGCCTGGAAGGCTTATCATCCAGGCCCGCGACTTCCCCCCAGAGTCTGAAGCCTTGCGA
CCTGGTCGGGGACGACAAACCTGCTCGGGGTCAACAGCGAGACCCTAGTGGGGAATGAGG
ATGGGTGAGGGTGCGCCTGGGTTGAGGGTCTGTGACTGTAGCATGGAAAAGGAGTTCATG
CAGGGATCGCACTGAGGGACAGCCAAGAATACTCCAGCGGCAGCCTCCCTCCTACCAATT
ACACGCCGCCTGCTGGCACAGTGGCACCTCGGGAACAGGACGCCCTTCCGGGCCCTGAAT
TGGGGCAGCGCAGGAGGGAGGGTGCAGAGCCATAGCGACCTGTGTGTACCATAGACATGC
ACATGCGCTCACCTTCACGTGTAACCCTTCTGAGTCCCAGACAGGGTAACTGGCGAGGCC
GTAAGTACCCTCGTGGGGGCGGAAATATTGCCGCGTCCCTGAGTCCTCTGGGCAGATGCA
GAGCCGCGTAAGCCTCATAGAAGCAAAGCCAGGGGAACAGTGGGAGCTCTGGCCTCGCGG
TGCGGACGCTAGTAATGGATTCCTGGGGAACAGCGACCTCTGGAGGAGCCTCTCATCCAC
ACAGAGTCAGGAACTAAAGGACTGGTTCTGAGACAAAGCACCCTTTCCTCACCCTACTCC
CATTCCACCCGCTTGGAAAAGTTATCCAGAGACTTTTAACCTCCGGACTCCACTCTTCGC
TTATGTTTGCTTTTTTTTCCAACCTGTACCCACGCTTCAAAATCCCTGCTCCAGTCCTGC
CTCTTCCAGAAAGAAGCCTTCTGGCCAGCCCAGTTCCTCTTCCCCTCTTCCTGTTCTGAG
CTCATTCTCAGGCCACACTGCACAGCCAACCTGCTTCCTGCCTGCTCTTCTCCAAAAAGA
CTGTGAATCCACTCCACCCACGCCTTGTGTTGCATGCTGGGCATGCCGTTCCCTGCCCCA
ACCCACCCCACCCCCCAGTGAATAAAGACTGAAGTTATTCATTCCAAAAAAAAAAAAAAA
AAAAAAA
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Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
Type(s) of Resistant Mechanism of This Molecule
  EADR: Epigenetic Alteration of DNA, RNA or Protein
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
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Gemcitabine
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Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Disease Class: Bladder cancer [ICD-11: 2C94.0] [1]
Resistant Disease Bladder cancer [ICD-11: 2C94.0]
 Disease Info 
Resistant Drug Gemcitabine
 Drug Info 
Molecule Alteration Expression
Up-regulation
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation FOXD2-AS1/miR-143/ABCC3 axis Regulation N.A.
In Vitro Model T24 cells Bladder Homo sapiens (Human) CVCL_0554
5637 cells Bladder Homo sapiens (Human) CVCL_0126
Experiment for
Molecule Alteration		 qRT-PCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description Results showed that lncRNA FOXD2-AS1 was high-expressed in gemcitabine-resistant bladder cancer cells. In vitro experiments, FOXD2-AS1 knockdown suppressed the 50% inhibitive concentration (IC50) of gemcitabine, drug-resistance related genes (MDR1, MRP2, LRP1) expression, invasion and ABCC3 protein expression in gemcitabine-resistant bladder cancer cells (T24/GEM, 5637/GEM). In vivo of xenograft assay, FOXD2-AS1 knockdown inhibited the tumor growth of bladder cancer cells. Bioinformatics program and validation experiments confirmed that FOXD2-AS1 positively regulated ABCC3 protein through targeting miR-143, acting as a competing endogenous RNA (ceRNA). In summary, our results revealed the vital roles of FOXD2-AS1/miR-143/ABCC3 axis in gemcitabine resistance of bladder cancer cells, providing a novel therapeutic strategy for bladder cancer.
References
Ref 1 Molecular Basis for Necitumumab Inhibition of EGFR Variants Associated with Acquired Cetuximab ResistanceMol Cancer Ther. 2018 Feb;17(2):521-531. doi: 10.1158/1535-7163.MCT-17-0575. Epub 2017 Nov 20.

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