Molecule Information

General Information of the Molecule (ID: Mol02029)

Name	FAD-containing monooxygenase EthA (ETHA) ,Mycobacteroides abscessus
Synonyms	ethA_2; I3U33_00520; SAMEA2071181_01734 Click to Show/Hide
Molecule Type	Protein
Gene Name	ETHA
Gene ID	66970488
Sequence	MSEHFDVLIVGAGISGISAAWHIQDRCPTKTYAVLEARDDMGGTWNLFKYPGIRSDSDMY TLGFRFSPWNDSRTLADGPSILDYVHKTAANAGIDGHVRYRQKVVGAAWNTETQQWTVEV DHDGKTIEYTCSFLFCCSGYYDYDQGYSPEFPGVADFKGTVVHPQHWPEDLDYKGKKVVV IGSGATAVTLVPAMAPDTGHITMLQRSPTYIMSLPNENPIINGLRKILPPKVAYPIARWI NIGQLIFSYQASRKFPRAARRIIMQQAKLQLPKGFDYKTHFGPKYNPWDERLCVVPNGDL YKAIRKGKADIVTDHIETFDETGIKLKSGKHLDADIIITATGLNLKFFSGVIPTVDGVPV DLPAQTVYKGAMLTGIPNMAFTIGYTNASWTLKADLVSEYVSRLLNYMDENGYVTAVPEL ADETLEKQPFMDFTPGYVLRALDELPKQGNKHPWRLKQNYAYDIGMMRRSRVTEGMRFGR KKTAAAPASESVAVNS Click to Show/Hide
Uniprot ID	A0A1N2GJY0_9MYCO
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Actinobacteria Class: Actinomycetia Order: Corynebacteriales Family: Mycobacteriaceae Genus: Mycobacteroides Species: Mycobacteroides abscessus

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Thiacetazone

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Mycobacterium abscessus infection			[1]
Resistant Disease	Mycobacterium abscessus infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Thiacetazone		Drug Info
Molecule Alteration	Expression	Down-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Mycobacterium abscessus strain CIP104536T		36809
	Mycobacterium bolletii strain CIP108541T		319705
	Mycobacterium massiliense strain CIP108297T		319705
Experiment for Molecule Alteration	Whole-genome sequencing assay
Experiment for Drug Resistance	Microdilution method
Mechanism Description	TAC, like ethionamide, requires activation by the flavin-containing monooxygenase EthA. EthR, whose gene is adjacent to ethA in M. tuberculosis and in M. smegmatis, represses the transcription of ethA, subsequently preventing the conversion of the prodrugs to active molecules. EthR belongs to the TetR/CamR family of transcriptional regulators that negatively regulates the expression of EthA.

References

Ref 1	Resistance to Thiacetazone Derivatives Active against Mycobacterium abscessus Involves Mutations in the MmpL5 Transcriptional Repressor MAB_4384 .Antimicrob Agents Chemother. 2017 Mar 24;61(4):e02509-16. doi: 10.1128/AAC.02509-16. Print 2017 Apr. 10.1128/AAC.02509-16