Molecule Information

General Information of the Molecule (ID: Mol01563)

Name	hsa-miR-196a-5p ,Homo sapiens
Synonyms	microRNA 196a-1 Click to Show/Hide
Molecule Type	Mature miRNA
Sequence	UAGGUAGUUUCAUGUUGUUGGG Click to Show/Hide
Ensembl ID	ENSG00000210741
HGNC ID	HGNC:31567
Mature Accession	MIMAT0000226
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s)

2 drug(s) in total

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Cisplatin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Disease Class: Bladder cancer				[1]
Resistant Disease	Bladder cancer [ICD-11: 2C94.0]			Disease Info
Resistant Drug	Cisplatin			Drug Info
Molecule Alteration	Expression		Up-regulation
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	5637 cells	Bladder	Homo sapiens (Human)	CVCL_0126
	UMUC-2 cells	Bladder	Homo sapiens (Human)	CVCL_8155
In Vivo Model	Nude mouse xenograft model			Mus musculus
Experiment for Molecule Alteration	RT-PCR
Experiment for Drug Resistance	Annexin V-FITC/PI Apoptosis assay
Mechanism Description	Long non-coding RNA UCA1 promotes cisplatin/gemcitabine resistance through CREB modulating miR196a-5p in bladder cancer cells. UCA1 upregulates miR196a-5p through transcription factor CREB.

Gemcitabine

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Disease Class: Bladder cancer				[1]
Resistant Disease	Bladder cancer [ICD-11: 2C94.0]			Disease Info
Resistant Drug	Gemcitabine			Drug Info
Molecule Alteration	Expression		Up-regulation
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	5637 cells	Bladder	Homo sapiens (Human)	CVCL_0126
	UMUC-2 cells	Bladder	Homo sapiens (Human)	CVCL_8155
In Vivo Model	Nude mouse xenograft model			Mus musculus
Experiment for Molecule Alteration	RT-PCR
Experiment for Drug Resistance	Annexin V-FITC/PI Apoptosis assay
Mechanism Description	Long non-coding RNA UCA1 promotes cisplatin/gemcitabine resistance through CREB modulating miR196a-5p in bladder cancer cells. UCA1 upregulates miR196a-5p through transcription factor CREB.

References

Ref 1	Long non-coding RNA UCA1 promotes cisplatin/gemcitabine resistance through CREB modulating miR-196a-5p in bladder cancer cells. Cancer Lett. 2016 Nov 1;382(1):64-76. doi: 10.1016/j.canlet.2016.08.015. Epub 2016 Aug 31.