Molecule Information

General Information of the Molecule (ID: Mol01125)

• Name: Tunicamycin resistance protein (TMRB) ,Bacillus subtilis
• Synonyms: BSU03140
• Molecule Type: Protein
• Gene Name: tmrB
• Gene ID: 938346
• Sequence:
  MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
  FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
  TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
  RRFADRLMVKLNHIRIK
• Function: Involved in the resistance to tunicamycin. Binds ATP.
• Uniprot ID: TMRB_BACSU

Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Species: Bacillus subtilis

Type(s) of Resistant Mechanism of This Molecule

  IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Investigative Drug(s) 1 drug(s) in total

Tunicamycin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Irregularity in Drug Uptake and Drug Efflux (IDUE)

Disease Class: Bacillus subtilis infection [1]

• Resistant Disease: Bacillus subtilis infection [ICD-11: 1G40.1]
• Resistant Drug: Tunicamycin
• Molecule Alteration: Expression; Inherence
• Experimental Note: Discovered Using In-vivo Testing Model
• In Vitro Model: Bacillus subtilis strain; 1423
• In Vitro Model: Escherichia coli strain; 562
• Experiment for Molecule Alteration: SDS-PAGE assay
• Mechanism Description: Overproduction of TmrB protein, a 22.5-kDa protein with an N-terminal ATP-binding region and a C-terminal amphiphilic alpha-helix, confers resistance to tunicamycin on Bacillus subtilis. TmrB protein was found to bind Sepharose 6B to which tunicamycin was covalently linked. Experiments with mutant proteins found that the C-terminal region of TmrB protein might be involved in the binding to tunicamycin.

References

• Ref 1: TmrB protein, which confers resistance to tunicamycin on Bacillus subtilis, binds tunicamycin. Biosci Biotechnol Biochem. 1995 Feb;59(2):321-2. doi: 10.1271/bbb.59.321.