Molecule Information

General Information of the Molecule (ID: Mol00882)

Name	Copper-translocating P-type ATPase (COPA) ,Mycolicibacterium smegmatis
Synonyms	D806_060860 Click to Show/Hide
Molecule Type	Protein
Gene Name	D806_060860
Sequence	MTTSTPVPAASIELQIGGMTCASCANRIERRLNKLDGVIASVNYATEKATVAVPDGYDPA DLISAVEATGYTASLPATSAPAREDTSPAAHDDPELDALRHRLLGSTVLSIPVIAMAMIP ALQFTYWQWASLTLAAPVVIWAAWPFHRAAWANLRHGSATMDTLISMGTLAAFLWSLYAL FLGTAGTAGMTHPFALALTPSHGAANIYLEVAAGVTTFILAGRYFEKRSKRQAGAALRAL LELGAKEVSVLRDGAETKIPVDRLAVGDEFVVRPGEKIATDGVVVSGTSAVDASMLTGES IPVDVGVGDTVVGATVNAGGRLVVRASRVGSDTQLAQMAQLVENAQTGKAQVQRLADRIS GVFVPIVIAVAVITLGAWLGAGYPAAAAFTAAVAVLVIACPCALGLATPTALLVGTGRGA QMGILIKGPEVLESTRTVDTVVLDKTGTVTTGKMALVDVRTIPGTQRRDLLRLAGALEDA SEHPIARAIATAARDEVGVLPTVEDFANAEGEGVHGVVDGHAVVVGRESLLTDWAQHLDT DLAQAKQHAEAEGKTVVTVGWDGTARGILIIADTVKPTSAQAITELKELGLTPILLTGDN DAVARRIATDVGIDTVIAEVMPEGKVQAITILQSQGKTVAMIGDGVNDAAALAQADLGLA MGTGTDVAIEASDITLVRGDLRSAVDAIRLSRKTLSTIKTNLFWAFAYNVAAVPVAALGM LNPMLAGAAMAFSSVFVVGNSLRLRGFTSTFTDRKDNGHE Click to Show/Hide
Uniprot ID	A0A2U9PZ75_MYCSE
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Actinobacteria Class: Actinomycetia Order: Corynebacteriales Family: Mycobacteriaceae Genus: Mycolicibacterium Species: Mycolicibacterium smegmatis

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

Click to Show/Hide the Full List of Drugs

Copper

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)		Click to Show/Hide
Disease Class: HIV-infected patients with tuberculosis [ICD-11: 1C60.0]			[1]
Resistant Disease	HIV-infected patients with tuberculosis [ICD-11: 1C60.0]		Disease Info
Resistant Drug	Copper		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
Cell Pathway Regulation	Cell growth	Inhibition	hsa05200
In Vitro Model	Mycobacterium smegmatis mc2155		246196
	Mycobacterium smegmatis mc2155-Cu		246196
Experiment for Molecule Alteration	qPCR
Experiment for Drug Resistance	MIC assay
Mechanism Description	Quantitative proteomics was carried out to find differentially expressed proteins between mc2155 and mc2155-Cu. Among 345 differentially expressed proteins, copper-translocating P-type ATPase was up-regulated, while all other ABC transporters were down-regulated in mc2155-Cu, suggesting copper-translocating P-type ATPase plays a crucial role in copper resistance. Results also indicated that the down-regulation of metabolic enzymes and decreases in cellular NAD, FAD, mycothiol, and glutamine levels in mc2155-Cu were responsible for its slowing growth rate as compared to mc2155.

References

Ref 1	Proteomic Analysis of Drug-Resistant Mycobacteria: Co-Evolution of Copper and INH Resistance. PLoS One. 2015 Jun 2;10(6):e0127788. doi: 10.1371/journal.pone.0127788. eCollection 2015.