Molecule Information

General Information of the Molecule (ID: Mol00197)

Name	E3 ubiquitin-protein ligase ZNRF2 (ZNRF2) ,Homo sapiens
Synonyms	Protein Ells2; RING finger protein 202; RING-type E3 ubiquitin transferase ZNRF2; Zinc/RING finger protein 2; RNF202 Click to Show/Hide
Molecule Type	Protein
Gene Name	ZNRF2
Gene ID	223082
Location	chr7:30284597-30367689[+]
Sequence	MGAKQSGPAAANGRTRAYSGSDLPSSSSGGANGTAGGGGGARAAAAGRFPAQVPSAHQPS ASGGAAAAAAAPAAPAAPRSRSLGGAVGSVASGARAAQSPFSIPNSSSGPYGSQDSVHSS PEDGGGGRDRPVGGSPGGPRLVIGSLPAHLSPHMFGGFKCPVCSKFVSSDEMDLHLVMCL TKPRITYNEDVLSKDAGECAICLEELQQGDTIARLPCLCIYHKGCIDEWFEVNRSCPEHP SD Click to Show/Hide
Function	May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Click to Show/Hide
Uniprot ID	ZNRF2_HUMAN
Ensembl ID	ENSG00000180233
HGNC ID	HGNC:22316
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Doxorubicin

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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)			Click to Show/Hide
Disease Class: Osteosarcoma				[1]
Sensitive Disease	Osteosarcoma [ICD-11: 2B51.0]			Disease Info
Sensitive Drug	Doxorubicin			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Identified from the Human Clinical Data
Cell Pathway Regulation	Cell growth		Inhibition	hsa05200
In Vitro Model	U2OS cells	Bone	Homo sapiens (Human)	CVCL_0042
Experiment for Molecule Alteration	Western blot analysis; Luciferase reporter assay
Experiment for Drug Resistance	MTT assay; CCK8 assay
Mechanism Description	Either ZNRF2 overexpression or miR100 depletion increased in vitro OS cell growth and improved cell survival at the presence of Doxorubicin. miR100 bindS to the 3'-UTR of ZNRF2 mRNA to prevent its protein translation, re-expression of miR100 may inhibit OS cell growth and decrease OS cell chemo-resistance.

References

Ref 1	MicroRNA-100 suppresses human osteosarcoma cell proliferation and chemo-resistance via ZNRF2. Oncotarget. 2017 May 23;8(21):34678-34686. doi: 10.18632/oncotarget.16149.