Drug Information

Drug (ID: DG00393) and It's Reported Resistant Information

• Name: N-methyl-n-nitro-n-nitrosoguanidine
• Synonyms: SCHEMBL1536062; SCHEMBL8581312; DTXSID00521861; 2-methyl-3-nitro-nitrosoguanidine; N-methyl-N'-nitro-N''-nitrosoguanidine; N''-Methyl-N-nitro-N'-nitrosoguanidine; 66542-13-0
• Formula: C2H5N5O3
• IsoSMILES: CN=C(NN=O)N[N+](=O)[O-]
• InChI: 1S/C2H5N5O3/c1-3-2(4-6-8)5-7(9)10/h1H3,(H2,3,4,5,8)
• InChIKey: POJDZWIPNZWPAU-UHFFFAOYSA-N
• PubChem CID: 13146027

Type(s) of Resistant Mechanism of This Drug

  EADR: Epigenetic Alteration of DNA, RNA or Protein

  UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Their Corresponding Diseases

ICD-02: Benign/in-situ/malignant neoplasm

Breast cancer [ICD-11: 2C60]

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Key Molecule: hsa-miR-891b [1]

• Molecule Alteration: Expression; Up-regulation
• Sensitive Disease: Breast cancer [ICD-11: 2C60.3]
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: HCC1806 cells; Breast; Homo sapiens (Human); CVCL_1258
• Experiment for Molecule Alteration: RT-qPCR
• Experiment for Drug Resistance: MTT assay; Annexin V assay
• Mechanism Description: Down-regulation of PARP1 by miR891b sensitizes human breast cancer cells to alkylating chemotherapeutic drugs. miR891b increased the sensitivity of the HCC1806 cells to the cytotoxic effects of MNNG through suppressing cell proliferation and increasing the percentage of apoptotic cells.

Unusual Activation of Pro-survival Pathway (UAPP)

Key Molecule: Poly[ADP-ribose] synthase 1 (PARP1) [1]

• Molecule Alteration: Expression; Down-regulation
• Sensitive Disease: Breast cancer [ICD-11: 2C60.3]
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: HCC1806 cells; Breast; Homo sapiens (Human); CVCL_1258
• Experiment for Molecule Alteration: Dual luciferase reporter assay; Western blot analysis; RT-qPCR
• Experiment for Drug Resistance: MTT assay; Annexin V assay
• Mechanism Description: Down-regulation of PARP1 by miR891b sensitizes human breast cancer cells to alkylating chemotherapeutic drugs. miR891b increased the sensitivity of the HCC1806 cells to the cytotoxic effects of MNNG through suppressing cell proliferation and increasing the percentage of apoptotic cells.

References

• Ref 1: Down-regulation of PARP1 by miR-891b sensitizes human breast cancer cells to alkylating chemotherapeutic drugs. Arch Gynecol Obstet. 2017 Sep;296(3):543-549. doi: 10.1007/s00404-017-4444-3. Epub 2017 Jun 28.