Disease Information

General Information of the Disease (ID: DIS00213)

• Name: Germ cell tumor
• ICD: ICD-11: 2D12

Type(s) of Resistant Mechanism of This Disease

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Cisplatin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Key Molecule: HIC ZBTB transcriptional repressor 1 (HIC1) [1]

• Resistant Disease: Germ cell tumor [ICD-11: 2D12.Y]
• Resistant Drug: Cisplatin
• Molecule Alteration: Methylation; Up-regulation
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: 833K-E cells; Ascites; Homo sapiens (Human); CVCL_2292
• Experiment for Molecule Alteration: Western blotting assay
• Mechanism Description: Promoter hypermethylation of RASSF1A and HIC1 genes play a role in resistance of GCT, while the transcriptional inactivation of MGMT by epigenetic alterations confer exquisite sensitivity to cisplatin.

Key Molecule: Ras association domain-containing protein 1 (RASSF1) [1]

• Resistant Disease: Germ cell tumor [ICD-11: 2D12.Y]
• Resistant Drug: Cisplatin
• Molecule Alteration: Methylation; Up-regulation
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: 833K-E cells; Ascites; Homo sapiens (Human); CVCL_2292
• Experiment for Molecule Alteration: Western blotting assay
• Mechanism Description: Promoter hypermethylation of RASSF1A and HIC1 genes play a role in resistance of GCT, while the transcriptional inactivation of MGMT by epigenetic alterations confer exquisite sensitivity to cisplatin.

Key Molecule: hsa-miR-518d [2]

• Resistant Disease: Germ cell tumor [ICD-11: 2D12.Y]
• Resistant Drug: Cisplatin
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: NTERA-2 cells; Lung; Homo sapiens (Human); CVCL_0034
• In Vitro Model: NCCIT cells; Embryo; Homo sapiens (Human); CVCL_1451
• Experiment for Molecule Alteration: RT-qPCR
• Experiment for Drug Resistance: Cell viability assay
• Mechanism Description: hsa-miR-518d potentially involved in cisplatin resistance of germ cell tumor cell lines.

References

• Ref 1: Role of promoter hypermethylation in Cisplatin treatment response of male germ cell tumors .Mol Cancer. 2004 May 18;3:16. doi: 10.1186/1476-4598-3-16. 10.1186/1476-4598-3-16
• Ref 2: The fibroblast growth factor receptor genetic status as a potential predictor of the sensitivity to CH5183284/Debio 1347, a novel selective FGFR inhibitorMol Cancer Ther. 2014 Nov;13(11):2547-58. doi: 10.1158/1535-7163.MCT-14-0248. Epub 2014 Aug 28.