Molecule Information

General Information of the Molecule (ID: Mol02148)

Name	GTPase HRas (HRAS) ,Mus musculus
Synonyms	Hras Hras1 Click to Show/Hide
Molecule Type	Protein
Gene Name	Hras
Gene ID	15461
Location	chr7:140,769,018-140,773,918[-]
Sequence	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPG CMSCKCVLS Click to Show/Hide
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Click to Show/Hide
Uniprot ID	RASH_MOUSE
Ensembl ID	ENSMUSG00000025499
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: 9989 Family: Muridae Genus: Mus Species: Mus musculus

Type(s) of Resistant Mechanism of This Molecule

UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Investigative Drug(s)

2 drug(s) in total

Click to Show/Hide the Full List of Drugs

GDC-0623

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)			Click to Show/Hide
Disease Class: Unclassified pleomorphic sarcoma				[1]
Resistant Disease	Unclassified pleomorphic sarcoma [ICD-11: 2B54.0]			Disease Info
Resistant Drug	GDC-0623			Drug Info
Molecule Alteration	Missense mutation		p.G12V
Experimental Note	Discovered Using In-vivo Testing Model
Cell Pathway Regulation	RAS signaling pathway		Activation	hsa04014
In Vitro Model	HEK293T cells	Kidney	Homo sapiens (Human)	CVCL_0063
	NIH3T3 cells	Embryo	Homo sapiens (Human)	N.A.
In Vivo Model	SCID/Beige mice model			Mus musculus
Experiment for Molecule Alteration	qRT-PCR; Western blotting assay
Experiment for Drug Resistance	Cell viability assay
Mechanism Description	Hras G12V mutation changed the drug target,impairing the ability to inhibit RAS-RAF-MEK-ERK signaling.

SCH772984

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)			Click to Show/Hide
Disease Class: Unclassified pleomorphic sarcoma				[1]
Resistant Disease	Unclassified pleomorphic sarcoma [ICD-11: 2B54.0]			Disease Info
Resistant Drug	SCH772984			Drug Info
Molecule Alteration	Missense mutation		p.G12V
Experimental Note	Discovered Using In-vivo Testing Model
Cell Pathway Regulation	RAS signaling pathway		Activation	hsa04014
In Vitro Model	HEK293T cells	Kidney	Homo sapiens (Human)	CVCL_0063
	NIH3T3 cells	Embryo	Homo sapiens (Human)	N.A.
In Vivo Model	SCID/Beige mice model			Mus musculus
Experiment for Molecule Alteration	qRT-PCR; Western blotting assay
Experiment for Drug Resistance	Cell viability assay
Mechanism Description	Hras G12V mutation changed the drug target,impairing the ability to inhibit RAS-RAF-MEK-ERK signaling.

References

Ref 1	Sensitivity and Resistance of Oncogenic RAS-Driven Tumors to Dual MEK and ERK Inhibition .Cancers (Basel). 2021 Apr 13;13(8):1852. doi: 10.3390/cancers13081852. 10.3390/cancers13081852