Molecule Information

General Information of the Molecule (ID: Mol00943)

• Name: DNA-directed RNA polymerase subunit beta 2 (RPOB2) ,Nocardia farcinica
• Synonyms: RNAP subunit beta 2; RNA polymerase subunit beta 2; Transcriptase subunit beta 2; NFA_50990
• Molecule Type: Protein
• Gene Name: rpoB2
• Gene ID: 61135673
• Sequence:
  MLEGRILAVSTQTKAVAGIPGAPKRVSFAKIREPLEVPGLLDLQTESFAWLIGSPEWRER
  AAARGDVGLVGGLEEVLEELSPIEDFSGSMSLSFSDPRFEEVKASIDECKEKDMTYAAPL
  FVTAEFINNNTGEIKSQTVFMGDFPMMTDKGTFIINGTERVVVSQLVRSPGVYFDHSIDK
  GTEKDLHSVRVIPSRGAWLEFDVDKRDTVGVRIDRKRRQPVTVLLKALGWTTEEIAERFG
  FSEIMMSTLEKDNTAGQDEALLDIYRKLRPGEPPTKESAQTLLENLFFKEKRYDLARVGR
  YKINKKLGIHVGEPVTGSVLTKEDIVTTIEYLVRLHAGDKTMTAPGGVEVPVEVDDIDHF
  GNRRLRTVGELIQNQIRVGLSRMERVVRERMTTQDVEAITPQTLINIRPVVAAIKEFFGT
  SQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHPSHYGRMCPIETPEGPN
  IGLIGSLSVYARVNPFGFIETPYRKVVDGRVTDEVVYLTADEEDRHVRAQANSPVGPDGR
  FLEDRVLCRRGNEEMEYVAATEVDFMDVSPRQMVSVATAMIPFLEHDDANRALMGANMQR
  QAVPLIRSEAPIVGTGMELRAAVDAGDVVVNEKAGVVEEVSADYVTVMADDGTRKSYRMR
  KFNRSNQGTCSNQRPIVDEGQRVEAGQVLADGPCTENGEMALGKNLLVAIMPWEGHNYED
  AIILSQRLVEQDVLTSIHIEEHEIDARDTKLGAEEITRDIPNVSDEVLADLDERGIVRIG
  AEVRDGDILVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGIRVF
  SREDDDDLPPGVNELVRVYVAQKRKIQDGDKLAGRHGNKGVIGKILPTEDMPFLPDGTPV
  DIILNTHGVPRRMNIGQILETHLGWIGKAGWKVEGNPEWAKDLPEEMWEAPADSNIATPV
  FDGAREEELTGLLGSTLPNRDGERMVDDNGKAVLFDGRSGEPFPYPVAVGYMYILKLHHL
  VDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAYTLQELLTIKSDDV
  VGRVKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGAAIELREGEDEDLE
  RAAANLGINLSRNEAATVDDLAN
• Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
• Uniprot ID: RPOB2_NOCFA

Kingdom: N.A.
Phylum: Actinobacteria
Class: Actinomycetia
Order: Corynebacteriales
Family: Nocardiaceae
Genus: Nocardia
Species: Nocardia farcinica

Type(s) of Resistant Mechanism of This Molecule

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Rifampin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Disease Class: Nocardiosis [1]

• Resistant Disease: Nocardiosis [ICD-11: 1C1B.0]
• Resistant Drug: Rifampin
• Molecule Alteration: Expression; Inherence
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Nocardia farcinica IFM 10152; 247156
• Experiment for Molecule Alteration: PCR; Southern analysis
• Experiment for Drug Resistance: Brain heart infusion (BHI) broth assay
• Mechanism Description: RpoB2 may have reduced binding affinity for RIF due to amino acid substitutions and is capable of functioning in the presence of RIF instead of RpoB. Besides, RNAP with RpoB2 may elicit expression of a latent RIF resistance gene which may be present in the genome.

References

• Ref 1: Contribution of rpoB2 RNA polymerase beta subunit gene to rifampin resistance in Nocardia species. Antimicrob Agents Chemother. 2006 Apr;50(4):1342-6. doi: 10.1128/AAC.50.4.1342-1346.2006.