General Information of the Molecule (ID: Mol00855)
Name
Cardiolipin synthase 2 (CLS2) ,Staphylococcus aureus
Synonyms
CL synthase 2; SACOL2079
    Click to Show/Hide
Molecule Type
Protein
Gene Name
cls2
Sequence
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGF
ILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLL
YNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKK
AEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISY
DDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLD
SIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDD
EIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL
    Click to Show/Hide
Function
Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol.
    Click to Show/Hide
Uniprot ID
CLS2_STAAC
        Click to Show/Hide the Complete Species Lineage
Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Staphylococcaceae
Genus: Staphylococcus
Species: Staphylococcus aureus
Type(s) of Resistant Mechanism of This Molecule
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
Click to Show/Hide the Full List of Drugs
Daptomycin
Click to Show/Hide
Drug Resistance Data Categorized by Their Corresponding Mechanisms
       Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Disease Class: Staphylococcus aureus infection [1]
Resistant Disease Staphylococcus aureus infection [ICD-11: 1B54.0]
Resistant Drug Daptomycin
Molecule Alteration Missense mutation
p.A23V+p.T33N+p.L52F+p.F60S
Experimental Note Identified from the Human Clinical Data
In Vitro Model Staphylococcus aureus isolates 1280
Staphylococcus aureus MRSA32 [A5948] 553567
Staphylococcus aureus RN6607 [A8115] 553573
Staphylococcus aureus RN9120 [A8117] 553574
Experiment for
Molecule Alteration
Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance
Broth microdilution method assay
Mechanism Description Mutation in each of these genes act similarly to reduce the net-negative charge of the cell membrane leading to electrorepulsion of daptomycin. They may act in isolation or in concert with each other, particularly for mutations in mprF and cls2.
Disease Class: Staphylococcus aureus infection [1]
Resistant Disease Staphylococcus aureus infection [ICD-11: 1B54.0]
Resistant Drug Daptomycin
Molecule Alteration Missense mutation
p.A23V+p.T33N+p.L52F+p.F60S
Experimental Note Identified from the Human Clinical Data
In Vitro Model Staphylococcus aureus isolates 1280
Staphylococcus aureus MRSA32 [A5948] 553567
Staphylococcus aureus RN6607 [A8115] 553573
Staphylococcus aureus RN9120 [A8117] 553574
Experiment for
Molecule Alteration
Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance
Broth microdilution method assay
Mechanism Description Mutation in each of these genes act similarly to reduce the net-negative charge of the cell membrane leading to electrorepulsion of daptomycin. They may act in isolation or in concert with each other, particularly for mutations in mprF and cls2.
Disease Class: Complicated skin infection Staphylococcus aureus infection [1]
Resistant Disease Complicated skin infection Staphylococcus aureus infection [ICD-11: 1B21.1]
Resistant Drug Daptomycin
Molecule Alteration Missense mutation
p.A23V+p.T33N+p.L52F+p.F60S
Experimental Note Identified from the Human Clinical Data
In Vitro Model Staphylococcus aureus isolates 1280
Staphylococcus aureus MRSA32 [A5948] 553567
Staphylococcus aureus RN6607 [A8115] 553573
Staphylococcus aureus RN9120 [A8117] 553574
Experiment for
Molecule Alteration
Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance
Broth microdilution method assay
Mechanism Description Mutation in each of these genes act similarly to reduce the net-negative charge of the cell membrane leading to electrorepulsion of daptomycin. They may act in isolation or in concert with each other, particularly for mutations in mprF and cls2.
Disease Class: Complicated soft tissue infection [1]
Resistant Disease Complicated soft tissue infection [ICD-11: 1B7Y.0]
Resistant Drug Daptomycin
Molecule Alteration Missense mutation
p.A23V+p.T33N+p.L52F+p.F60S
Experimental Note Identified from the Human Clinical Data
In Vitro Model Staphylococcus aureus isolates 1280
Staphylococcus aureus MRSA32 [A5948] 553567
Staphylococcus aureus RN6607 [A8115] 553573
Staphylococcus aureus RN9120 [A8117] 553574
Experiment for
Molecule Alteration
Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance
Broth microdilution method assay
Mechanism Description Mutation in each of these genes act similarly to reduce the net-negative charge of the cell membrane leading to electrorepulsion of daptomycin. They may act in isolation or in concert with each other, particularly for mutations in mprF and cls2.
Disease Class: Right-sided endocarditis [1]
Resistant Disease Right-sided endocarditis [ICD-11: BB41.0]
Resistant Drug Daptomycin
Molecule Alteration Missense mutation
p.A23V+p.T33N+p.L52F+p.F60S
Experimental Note Identified from the Human Clinical Data
In Vitro Model Staphylococcus aureus isolates 1280
Staphylococcus aureus MRSA32 [A5948] 553567
Staphylococcus aureus RN6607 [A8115] 553573
Staphylococcus aureus RN9120 [A8117] 553574
Experiment for
Molecule Alteration
Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance
Broth microdilution method assay
Mechanism Description Mutation in each of these genes act similarly to reduce the net-negative charge of the cell membrane leading to electrorepulsion of daptomycin. They may act in isolation or in concert with each other, particularly for mutations in mprF and cls2.
References
Ref 1 Whole genome characterization of the mechanisms of daptomycin resistance in clinical and laboratory derived isolates of Staphylococcus aureus. PLoS One. 2012;7(1):e28316. doi: 10.1371/journal.pone.0028316. Epub 2012 Jan 6.

If you find any error in data or bug in web service, please kindly report it to Dr. Sun and Dr. Zhang.