Drug Information

Drug (ID: DG00490) and It's Reported Resistant Information
Name
Huaier
Drug Resistance Disease(s)
Disease(s) with Resistance Information Discovered by Cell Line Test for This Drug (1 diseases)
Breast cancer [ICD-11: 2C60]
[1]
Type(s) of Resistant Mechanism of This Drug
  EADR: Epigenetic Alteration of DNA, RNA or Protein
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Their Corresponding Diseases
ICD-02: Benign/in-situ/malignant neoplasm
Click to Show/Hide the Resistance Disease of This Class
Breast cancer [ICD-11: 2C60]
Click to Show/Hide
Drug Resistance Data Categorized by Their Corresponding Mechanisms
       Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Key Molecule: H19, imprinted maternally expressed transcript (H19) [1]
Molecule Alteration Expression
Up-regulation
 Molecule Info 
Resistant Disease Breast cancer [ICD-11: 2C60.3]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Inhibition hsa04210
Cell metastasis Activation hsa05205
Cell proliferation Activation hsa05200
Cell viability Activation hsa05200
Tumorigenesis Activation hsa05206
In Vitro Model MCF-7 cells Breast Homo sapiens (Human) CVCL_0031
MDA-MB-231 cells Breast Homo sapiens (Human) CVCL_0062
MDA-MB-468 cells Breast Homo sapiens (Human) CVCL_0419
Experiment for
Molecule Alteration		 qPCR; Microarray assay
Experiment for
Drug Resistance		 Flow cytometry assay; MTT assay
Mechanism Description CBL was a direct target of miR675-5p, overexpression miR675-5p decreased the expression of CBL. H19/miR675-5p increases cell proliferation by regulating CBL. Huaier extract reduced the expression of H19 and miR675-5p, thereby inhibiting breast cancer progression through increasing CBL.
       Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Key Molecule: E3 ubiquitin-protein ligase CBL (CBL) [1]
Molecule Alteration Expression
Down-regulation
 Molecule Info 
Resistant Disease Breast cancer [ICD-11: 2C60.3]
 Disease Info 
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation Cell apoptosis Activation hsa04210
Cell apoptosis Inhibition hsa04210
Cell viability Activation hsa05200
In Vitro Model MCF-7 cells Breast Homo sapiens (Human) CVCL_0031
MDA-MB-231 cells Breast Homo sapiens (Human) CVCL_0062
MDA-MB-468 cells Breast Homo sapiens (Human) CVCL_0419
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 Flow cytometry assay; MTT assay
Mechanism Description CBL was a direct target of miR675-5p, overexpression miR675-5p decreased the expression of CBL. H19/miR675-5p increases cell proliferation by regulating CBL. Huaier extract reduced the expression of H19 and miR675-5p, thereby inhibiting breast cancer progression through increasing CBL.
References
Ref 1 Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway. Cell Physiol Biochem. 2017;44(2):581-593. doi: 10.1159/000485093. Epub 2017 Nov 17.

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