Drug Information

Drug (ID: DG00376) and It's Reported Resistant Information

• Name: Streptothricin
• Synonyms: STREPTOTHRICINS; Streptothricin; 54003-27-9; EINECS 258-911-6; DTXSID60202321; [6-[[(3aS,7R,7aS)-7-hydroxy-4-oxo-1,3a,5,6,7,7a-hexahydroimidazo[4,5-c]pyridin-2-yl]amino]-5-(3,6-diaminohexanoylamino)-4-hydroxy-2-(hydroxymethyl)oxan-3-yl] carbamate; [6-[(E)-[(3aS,7R,7aS)-7-hydroxy-4-oxo-3,3a,5,6,7,7a-hexahydro-1H-imidazo[4,5-c]pyridin-2-ylidene]amino]-5-(3,6-diaminohexanoylamino)-4-hydroxy-2-(hydroxymethyl)tetrahydropyran-3-yl] carbamate
• Drug Resistance Disease(s):
  Disease(s) with Clinically Reported Resistance for This Drug (1 diseases)
  Campylobacteriosis [ICD-11: 1C40]; [1]
  Disease(s) with Resistance Information Validated by in-vivo Model for This Drug (3 diseases)
  Actinomycetoma [ICD-11: 1C43]; [2]
  Escherichia coli intestinal infection [ICD-11: 1A03]; [2]
  Sepsis [ICD-11: 1G40]; [2]
• Formula: C19H34N8O8
• IsoSMILES: C1[C@H]([C@@H]2[C@@H](C(=O)N1)N=C(N2)NC3C(C(C(C(O3)CO)OC(=O)N)O)NC(=O)CC(CCCN)N)O
• InChI: 1S/C19H34N8O8/c20-3-1-2-7(21)4-10(30)24-13-14(31)15(35-18(22)33)9(6-28)34-17(13)27-19-25-11-8(29)5-23-16(32)12(11)26-19/h7-9,11-15,17,28-29,31H,1-6,20-21H2,(H2,22,33)(H,23,32)(H,24,30)(H2,25,26,27)/t7 ,8-,9 ,11-,12+,13 ,14 ,15 ,17 /m1/s1
• InChIKey: NRAUADCLPJTGSF-WJPMJIHPSA-N
• PubChem CID: 475825

Type(s) of Resistant Mechanism of This Drug

  DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Their Corresponding Diseases

ICD-01: Infectious/parasitic diseases

Escherichia coli intestinal infection [ICD-11: 1A03]

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Key Molecule: Streptothricin acetyltransferase (STA) [2]

• Molecule Alteration: Expression; Inherence
• Resistant Disease: Escherichia coli infection [ICD-11: 1A03.0]
• Experimental Note: Discovered Using In-vivo Testing Model
• In Vitro Model: Streptomyces lividans strain Tk21; 1916
• In Vitro Model: Bacillus subtilis strain RM141; 1423
• In Vitro Model: Escherichia coli strain 5131-5; 562
• Experiment for Molecule Alteration: [a-32P] dCTP by the dideoxynucleoside triphosphate chain termination method assay
• Mechanism Description: The nucleotide sequence of the streptothricin acetyltransferase (STAT) gene from streptothricin-producing Streptomyces lavendulae predicts a 189-amino-acid protein of molecular weight 20,000, which is consistent with that determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme. By addition of promoter signals and a synthetic ribosome-binding (Shine-Dalgarno) sequence at an appropriate position upstream of the STAT translational start codon, the STAT gene confers streptothricin resistance on Escherichia coli and Bacillus subtilis.

Campylobacteriosis [ICD-11: 1C40]

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Key Molecule: Streptothricin N-acetyltransferase Sat4 (SAT4) [1]

• Molecule Alteration: Expression; Inherence
• Resistant Disease: Campylobacter fetus infection [ICD-11: 1C40.0]
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Campylobacter coli strain BE/G4; 195
• In Vitro Model: Campylobacter coli strain BE5698; 195
• In Vitro Model: Campylobacter coli strain BE6361; 195
• In Vitro Model: Campylobacter coli strain BM2509; 195
• In Vitro Model: Campylobacter coli strain Ck196; 195
• In Vitro Model: Campylobacter coli strain Ck197; 195
• In Vitro Model: Campylobacter coli strain Ck199; 195
• In Vitro Model: Campylobacter coli strain Ck204; 195
• In Vitro Model: Campylobacter jejuni strain Ck194; 197
• In Vitro Model: Escherichia coli strain SURE; 562
• In Vitro Model: Escherichia coli strain SURE (pAT132); 562
• Experiment for Molecule Alteration: SDS-polyacrylamide gel assay
• Experiment for Drug Resistance: MIC assay
• Mechanism Description: The sat 4 streptothricin resistance gene from Campylobacter coli BE/G4 was cloned into pUC18, and its nucleotide sequence was determined. Streptothricin acetyltransferase activity was detected in Escherichia coli cells containing recombinant plasmid pAT132 which carries the sat4 gene as an insert. The deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from Escherichia coli and streptothricin producers Streptomyces lavendulae and Streptomyces noursei. The sat 4 gene was detected by hybridization in clinical and environmental isolates of Campylobacter spp.

Actinomycetoma [ICD-11: 1C43]

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Key Molecule: Streptothricin acetyltransferase (STA) [2]

• Molecule Alteration: Expression; Inherence
• Resistant Disease: Streptomyces lavendulae infection [ICD-11: 1C43.13]
• Experimental Note: Discovered Using In-vivo Testing Model
• In Vitro Model: Streptomyces lividans strain Tk21; 1916
• In Vitro Model: Bacillus subtilis strain RM141; 1423
• In Vitro Model: Escherichia coli strain 5131-5; 562
• Experiment for Molecule Alteration: [a-32P] dCTP by the dideoxynucleoside triphosphate chain termination method assay
• Mechanism Description: The nucleotide sequence of the streptothricin acetyltransferase (STAT) gene from streptothricin-producing Streptomyces lavendulae predicts a 189-amino-acid protein of molecular weight 20,000, which is consistent with that determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme. By addition of promoter signals and a synthetic ribosome-binding (Shine-Dalgarno) sequence at an appropriate position upstream of the STAT translational start codon, the STAT gene confers streptothricin resistance on Escherichia coli and Bacillus subtilis.

Sepsis [ICD-11: 1G40]

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Key Molecule: Streptothricin acetyltransferase (STA) [2]

• Molecule Alteration: Expression; Inherence
• Resistant Disease: Bacillus subtilis infection [ICD-11: 1G40.1]
• Experimental Note: Discovered Using In-vivo Testing Model
• In Vitro Model: Streptomyces lividans strain Tk21; 1916
• In Vitro Model: Bacillus subtilis strain RM141; 1423
• In Vitro Model: Escherichia coli strain 5131-5; 562
• Experiment for Molecule Alteration: [a-32P] dCTP by the dideoxynucleoside triphosphate chain termination method assay
• Mechanism Description: The nucleotide sequence of the streptothricin acetyltransferase (STAT) gene from streptothricin-producing Streptomyces lavendulae predicts a 189-amino-acid protein of molecular weight 20,000, which is consistent with that determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme. By addition of promoter signals and a synthetic ribosome-binding (Shine-Dalgarno) sequence at an appropriate position upstream of the STAT translational start codon, the STAT gene confers streptothricin resistance on Escherichia coli and Bacillus subtilis.

References

• Ref 1: Characterization of the sat4 gene encoding a streptothricin acetyltransferase in Campylobacter coli BE/G4. FEMS Microbiol Lett. 1994 Jul 1;120(1-2):13-7. doi: 10.1111/j.1574-6968.1994.tb07000.x.
• Ref 2: Nucleotide sequence of the streptothricin acetyltransferase gene from Streptomyces lavendulae and its expression in heterologous hosts. J Bacteriol. 1987 May;169(5):1929-37. doi: 10.1128/jb.169.5.1929-1937.1987.