Molecule Information

General Information of the Molecule (ID: Mol05640)

• Name: SOX2-OT ,Homo sapiens
• Molecule Type: Long non-coding RNA
• Sequence:
  AAAAATGTTGAATATCAACAATGGGTTTCAGTGCTGTATTGTTGAGTTTTGTATGAACTA
  ATCTAGCTTCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAAGAAAGAAAGAAA
  AGAAAGAAAGAAAGAAAAAAAAAGAAAAGAAAAAAAAGAGAACCACAGCTTTGGACAAAA
  GTCTATAGCCAGTGCATTTGAAAACTCCCCTTGCACCAGGGCTGACTGTAGCTGGGGGAA
  GACATTTTACTCATTTGCTCTTTCCTTTCATACAGTCAGCTGGTCCCTCTTCTCAGGTGT
  AGATCACCTATTATAATTTTACCTAATTTTGATACTCTGATAAGGAAAGTCCCAGGACTT
  ATCAGCTGGGATAGGCCTCACTTACAAGACAGCTCTGTTCAGTATTTGGAAGAAAGCCTG
  ACAGGTTTCTTCGGAAAAGTGGCCATCCATGGAATGAATGAAATGTTCTCTTTCTATTCC
  AGGGATTGCAGTGGCAAAGCTAGGCTAGGTCTTGGAGGCTGGTGTAAGGCGATGTGGGTG
  AAGGCAGGAGGCTGATGGAAAGACTGGGGGGAAGAAAAGCCGAAATGGATTCACGGTGCC
  TTGGATGAAGGACGAGAGGGGAACTGCAAGCTCCTTCAACTGGTTCTGTCCGGTGAGAAG
  TGATCAAGCTTGGGCTGACAAGAGGCTCAGGGAGCCCTCACGTTCTTTCGCTTTTTTACC
  TGCCAATCAAACTGCTACAAGACAACACCCTGATCTGGCATGGACATCGCGGGTCCAAGC
  CTGTAGCCCCAAATCGGATAATCTCTGCAGCTGATAACAAGCAAAAGAGAAGCCAGGCAA
  CAGCCATATTAAAGAAGAAAACAATCAACTCTGAGATCCAACTTAGAAATAATGTTTCAT
  TCAAGATAAGGCTCGTGGCTTAGGAGATTGTGACCTGGCTTGCATCATTCTAAGACTTCT
  ATCGTCTGTTTTCAAAACCCAAGGAGGACCTCCTTTTCTGTGTGATAGTTCCTCATGCTT
  TGCCAGCCACTGGGTCTTTAAGGAAGCTTGCCAAGAGTTCCCGGCTGGGAAGGACAGTTC
  GAGTTCATCATAGACAATGAGCTGGCACCACAGTTTAAGTGACTCACCAACCTGGTTGCC
  CACTTCAAAATGTCAAGAACCACACTATCTAATTGAAAGAACTGTGTTTTAGCAAAGTGT
  AATTGGATCGCCTGGCAAGATCATCTCAAACTATGTCTCTGACCTTGCCTTTCAGTGGCT
  ATTTTGAGCACAAGAGGAGAAAGGAAATGATTCCTGAATTATATCTTGTTTTTAAAAATT
  AAGATTAATTAATACTTGGTTTTATGTTCATTCCATTGTAGGAACTTAGTGATTATTACA
  AAACAGTCAAAATAGGTCATAGCAAAATGAACGTTTAAGATTAGGTTAAAACATTCCTCT
  GAAATTTTAGTATTCACTTTTGAAGTGTCAATTACTATATTTCTGTAATTTCTCATTTTT
  TTCTTGGCTTAGAGTCAAGATGAAAAATGATATTTGAATCAAAGACATTGTTTTGTTATA
  TAGCCACTCCTTTCTAAAATGCAAATCCACCCTCCCCTCACCAATGCTTTATTCTTTGTG
  CGCGCACACACACACACACACACACACACACACACACACACACACAGACATACACAGAAC
  CAGCACCCAAATGGTAGAGGAGGTCCTTCCAAGACTTCTAGGACCTGGATCATGTAAGCA
  GTAGATGAGAAAATGAAGTAAGAGATATAGAGTAATAACAATAAAAGTCAATGATTTCAT
  TTTACAAAAAAGTCATTGTGTAGTACTCAGATATTTTTAAGAGCAAGTATTATTATGACT
  AAAGTATATTATACTTCTAAATTATTATGATTCTCATCTAATTTCAAGCCACACATAGGC
  GCCATTTGAAAGCATTTTGTGTTACAACCTATCAACAAAAATCAACTAATAGTTTTATAA
  CGTGCATAGGTTTACCCCATGGGGAATTAAAATTTTTATCTGCAAGTATTTCTGTTGTTG
  TGAGACTTTAGGACAAGACAACATTTGGTGTTAACATAGAAGAATATTCTATGGCTTTTT
  TAGCTTTAAAGTTCATTACTCTAAAATAAAGTACACGTGGTATTTTTTTCTTCCTAGTGT
  TCTCTTATTATATGTAGAGTATTTTCGGTTGTTGTTTTTAACGTATACATTATTTTTATT
  AAGTCATTTCATAAAAGATAAGATATATACTAATAGACCCTTGGTTTTCTCCTAATTTAA
  TTATTTCAAGTCAAGCAAAATTGGGCAATTTTCTTTCAGGAAAAAAAAAATATTGAGCAT
  GATTTTGAGGTAAAACTGATTATTTACAGCATCTTTTCTCTAATAGGCTAACATTTATGA
  TGGATCTTATTTCAAAGTTGATTATTCTCAAAGAAAAGTGGAGATATTAGAATTTCCGAG
  TAAATTGCATTGTTTTAAAAAGTACTTTAATGCATTGTTCACATTGTTGCCAGCTATTGA
  CCAAAAAAGAGAAGAAGAAAAAAGAAATAAAAAAAGCAGTATCTGCTCCAAACGTCAAGT
  TTTGCAGTTTGAAAGACTTGTACATTATAATTGTTTTTTTCTTTTTTGGAAAGCAGTAGT
  AATTAATACCAAAGGCATAGACAATTTAGGGCCATAACCTCCCTTCCCCCCTCTCCACCT
  TTTTTTTTTTTTTTTTTTTTTTTTAACTTTAACCATGATAGGCATCTCTGCAAGGTAGGT
  TTTAACATATTTTTTTCCTAATTTTTTTCCCTCCATTCAGTAAGAAGGATAAGCTTTTAG
  AGTACCCAGTCACCGTGGTAACTGCCAAAAAAATTCTCCTGAAGTTTGAAGAGGTCAGCT
  AGCAGGGTCAACAACCCTGGCCTCTCTCTGAGCTCGGTCTAGCATGCCCCAGCCTGCGGT
  TGGAGTGTCAGGCCAGATCTGACCCTGGGAGGTGGACCATTCTGGCTCTGATATAAATTT
  TTCGAGTCAGTTCATGGCCTGGACTCTCCAGGTGGCCTCCAAAATCGATTTTGACCCCCT
  GACCCTGTCTCACGGAGGCATAGCATCCACCCTAAGTAAGGATTTAGCCAGGAATGACAG
  CTTTCCACTGAGTGTTATTGGGGACAGTATAAACAGTTTGGAAAGCAAGTAAGGAGGTGT
  GAGGGCTAGCAATCATTAAAAGCACATTAAAAAACAATTTTTAAAAATCTTTATTAGAGA
  AAGTTGTAAATGCATATCTGTTACAGAATTCCTGTGATTTGTAGAGTTTTCTAGTCAGTT
  TTGAATATAAATAGGTCACATATCTTTATCTTTTTGCATACTTTGTTACAAATATGCAAA
  TAAGATAGGCATACTTGCTACAAAATAGGTACAGCAGGAATGTAATATGTTCAGAAAAGG
  CAAACTGGTTATTAAAACACATTAACAGGTAAGGAGTTTCTAATATTTTAAATACTAAAA
  TTTTACATGTGTATTTTGAAGTTTTTAAAATGGAAAAATAAAGAACCTTTAAAAA

Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Temozolomide

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Disease Class: Glioblastoma [ICD-11: 2A00.02] [1]

• Resistant Disease: Glioblastoma [ICD-11: 2A00.02]
• Resistant Drug: Temozolomide
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• Cell Pathway Regulation: Wnt5a/beta-catenin signaling pathway; Regulation; N.A.
• In Vitro Model: U87 cells; Brain; Homo sapiens (Human); CVCL_0022
• In Vitro Model: U251 cells; Brain; Homo sapiens (Human); CVCL_0021
• In Vivo Model: Glioblastoma patients; Nude mouse xenograft model; Homo sapiens
• Experiment for Molecule Alteration: RT-qPCR; Western blot; Immunohistochemistry staining; RNA pulldown assay; RNA immunoprecipitation assay; MeRIP-qPCR
• Experiment for Drug Resistance: CCK8 assay; Flow cytometric
• Mechanism Description: Mechanistically, further investigation revealed that SOX2OT recruited ALKBH5, which binds with SOX2, demethylating the SOX2 transcript, leading to enhanced SOX2 expression. Together, these results demonstrated that LncRNA SOX2OT inhibited cell apoptosis, promoted cell proliferation, and TMZ resistance by upregulating SOX2 expression, which activated the Wnt5a/beta-catenin signaling pathway.

References

• Ref 1: Indian J Med Paediatr Oncol. 2015 Apr-Jun;36(2):133-6. doi: 10.4103/0971-5851.158852.