Molecule Information

General Information of the Molecule (ID: Mol05575)
Name
MSC-AS1 ,Homo sapiens
Molecule Type
Long non-coding RNA
Sequence
AGGATTTTTCCCAAGCCGGGAGAGCTGGCAGATCAGAGAGGGACTTTCCAGATAAATCCC
ATCTGGGGAAACGTTACTATCTTCCACAAGCAATTTTTCTAAAAATGCAAATGCTCTTTT
ATAGAGCTCGGCCTTCCCTCCTTTAAGAAAGGGCTTGCCGATTCAAACCTGCCCGACTCA
GCTGGATTAAGTTGGGCAAGGGAGATTTGCCGTCAGTCTGCAACCTGGTGGCAGATGGAG
ATGGAACCAAGATTTACCCTGGAAGTAGGATATTAGTCAATGGCTGTCACTGGGGTACAA
TGTTCAGAACGATGCAAACAGACACGGAGGGTTGATTCTTCTTCAGGGAAATGGCTGGCC
ACTCCCTTGATTTGGGAGAAACTAAACTGGCCTTCTCGTTCCCAGTCCCAGAACCTCCAC
CCCTTTCGAATTCTTCCCAACGGGCTGACCCTGCCCGGCGCCCAGGAGCGCCCTGGGTAT
CTCCTGGCTGCTCTCCCGAATCCTTGCGCGCCGCGCCCCTACCAGGTTCACTGGGTGCAC
GTAGCCGTTCTCATAGCGGTCCTCCTGCAACAGCTGCCGCAGTCTCGCTGTCTCCGCCTT
CCGCTCCCTGGCGGAGGCGGAGGCCAGAGAGCGCTCCAAGGAAGACTAAAAACCCAGGCC
GGGAAGCGCGGGGTGAGAAAGCGAGGTGGGTGGCGAGAGCGTGAGCGCCCCTCTGCTGAC
CCCGGGGAGCGTGGACTACGAGTTGGCGCCCAAGTCCAGAATCCGCGCGCACCGCGGAAA
ATATTTGCAATACCTGCCACGTCCAAGGTTCAGCTGAGGCCAATGTAAAGCCAGTATTAT
AGGACACTTCTCAGACTGAGAGCCCAATGTCGTTGGAAACCACTGGGCCACAGGAAAGGC
AGGCTCTCTCAGTCCTGCTCCTGCCATGGAAGAAGCCTGCTCCCACCATGCCTTCTGCCA
CGAGTAAAAGTTCCCTGAGGCCTCCCCAGAAGCAGATGCTGTCGTGCTTCCTGTATAGCT
GCAGAACTACTTCAAATCATCCCAACACCAGAGAACACTAAATGACAGTGGACAGGAAGA
CAGTGTACCTTTTGCTCCTGATGATCGTAAGAACAACCTCCTTCCTCTTATTGTGAAGTG
CAAGGTGCAAAAAGGAGGCCTGCTGCTTGTTCAGGACTATGTCAGCATTGTGGCTCAGAA
GAAGCGCAACGGCTTTGGCGTGGCCTTCCCTTGCAGCAAAGTGAAGTGCAGTGTTCTTTT
GAAGAAAAACAGACACAGAAAACGTGGTGACAGTGTCTAATCTGTCTTATTTCACTGCTT
AACTGCTTAAAAGACATTAGCGACACTATTTAATACCAAAATACATAGCACTTAAAACTG
GTGTTCAGAATCAAGCTTCAAAATAATGAGTTTATAGTTCTAGCATGGTGTGTGTGCATG
TGTGGGAAGTGGATCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGATAGAGAAAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATGAATTCCAAGGCATTTGTTTTGTG
TTGCTTTTGCAATGGGATGATTCCACAAGGACAAAGGACAAGCTTTCAGGGTAAAGATGT
TGGGTTGAACACAGAAGAAAATCCTCCTGAGATTGATCATTTGTAGAACAGTGGCTTACA
GATTTGGGGCTTCACAAGCTTGTAAATGTTTTTTAAAATCAGAGACTGTTATAAGTTTGC
CCATTTTTAGTTTCTCCACTTAAAGACTTTTTTTTTTTGAAAAAGGCAACAACAGTCTTC
TATCCCCATCATTTTATGAACAAACGTACATTTCAAAAGTTACTCTCCCCAAAGGAAGGA
AAGACATAATAGCACAATAAAGGGCAGCATTTCAAATCAATACACTTCAAAATAACACTT
ATCTTAATGAAAACTCACCCCTGCCTTCATTTCTCTGGACCTACCAGGGAGTAAACGCTT
TACCATCAAATTTCATTGGCCTGTGGACCTCGCTGCTCTGTGAGACTGGAGGTTTCCCCA
ACATTCACCCACCCCTTGGTATTCTCCCAATTCTGTTGCCTTTTGATTCATACTTCACCT
CAAGAATCCACTTGCTAAACACCCAACAAAAGGCTGGGAATAGAGCAAGGAAGGTAAAAG
GTGTCTAGGAGAGTTTTCTGGCAATGTTGGAAGTTTCTGCTATATTGAGATTTTGGGTAA
GCATGAGGACCGCAGTACATACCCCGTCTTCATCCAGGCGATCTGTGCACTTCAAATTAG
TATCAAGAATGACCTTCATGGTCTGAGTGTACCCGCCCATGGACGCATGATGCAAAGCTG
TCCAGCCATTGTGGTCACTGGTAAAGAGTTAAGAAAAGATGTGTGCATACACTTAACAGA
TGAAATGCGGGATGGGATGCCACTTGGCTTATTCTAGGTAAATCTGATGACAACAAAAAG
CATTATTGAGATCTGGCAACTAAATATTTATAAAACATTTATAATAAATGAGCTAATTCC
AAGCTCAATGATCTTAAATGACCTATTTCTAATGTGATTCATACACGTTTTTTACTTAGT
GGTTTAAAATATGAAAGTATATAAACACATTACCAAACACTTTGGGGTAGGAGAAGTACG
CTTAGCATGTCTGTGTCATACCTAATTTTTTTAAAGCTATAATGAACTGATACACATCAA
TTTAAATCTTGTTTTATTCTTTCAATGAACACTGTATCAAAATTTTTTTATGAAGTAATG
TTAGTCTTTGTGGTCAATAGTTATTGGTAATACTACTAGTAATATGATACATGGCTATAA
GATGCTTAGCTTAGTGTCAGTTATTATTCTAAGAGTTTAAATCTAAAAAGCTTCCCTCTT
GTGCTCTGAAAAATGCTTACTCAATTGCTTTGAAAAATGTTTATCCTTTTCCACAGAAAT
TCAAGATGTAAATACAACAAAGCCTTGTCATATTAGCTATCTTTTTTCAAATTAGACAGA
AAAAAATTCTTGACACTATTTAATTTCAGTGCTAGTGAGCAGATGTTTTAGGGTGCAAGA
GCCATGTTGCTATTTCAGTTATGATGTAATTAAGCAACAACTGTCTGGCCTGGGTCTACT
TCGGTCCCCTTTGCAGGTCAGTTGCTTGGTGTTTCTTTGTAAACCAGAAAAAACTGAGCA
AAACATTCATTCATGTAGCTGTAGCACCAATTTGCTGGCATCAATATTATGGTAAGGAAA
TGTGTTTTTGAACATTAGCCTAATAAGCTCTTTGCTACTGCTATGGTAAGATATCAAATG
AAAAACCTCTGTAAAACATTTCCAGGTTTTTTCTTTCTTATCACAATATCATTTAATTAT
GCCACATATTTTATTATACTTACATATTTTAACTTTGTTTCTTGTACTTGAGTTCATTTA
AGTATAATAAAATTCAGAAATTTTTACCATCTCCTGTTGTCACTTAAAGGGAACTTTTTA
TGCCTACCATTCATCAGATTGAATGAAAAGTAGAGTATTTGAACTTGTATGTTTTGTGTG
TGTGTGTACGTGCATACACACATACACAACCACAGTTTTGTATCTATATAGGAATATGGC
AGCATGATGAGGTAAAATATGAGAAACTATGTTTCAAAGAAAGGTTCTGAGAAAGACTGC
ACAAAGCCAATTATTCTGTGAACAGAATATTCTTGAACAAGGTTCTTCAACGCTATAAAC
TTCAAAGTCTTTGTCAGTTGGAAATAATACTGACTTCCCTTTCAGTATTTTTAGAAGTTA
CATATGTTTTGATGAAAAAATAAATTGGATAAAAGTACTTAGTGAGATATAAAGTTAGAC
CTCATAATTAAAAATATAATGAAAAGATAGCCTGAAAATGGCTAATTAAAGAAATTATAT
AAACACTCCAATCATATATCCTCACCTGAGAAACAATGCACCTTTTTTCAGAAGAAGCTG
AACTACTTTATCATGTCCATTCTTTGCTGCCAGATGGAGAGGAGTCATTCCATGAAGGTC
ACCTTCATTCAGAAGCCTCGTATCACTTATGTCTTGTAGGAGCCTCTGACAGGTATTGAT
ACGCCCATAACTTGGAAAAAATTAGGTTTCATATTTTCAAGGCAAATATTAAACAGAAAG
AAGACATGTTCATACATTGCTAGACTGACCCTTACCTGGCTGCAAAATGCAGAGGTGATT
TCTTATCTTTGCTTTTGGAATGAATGGACACATTAAAGCCAAGTAGGTTATTTACAGAAC
CAGGGCCCCCCTGTCTACATGCATAATGTAGAGGAGTACACCCATCGTTGTCTTCATCCA
TTACCAGCTCTTTGATCTGTTGCATCTATAGGAAAAAATTAATATCATACAAATAACTCT
GCTATTATGCTAATATGATGGTTTTCAAACTATTCTGTAGGAAAATATTTTCCAACAAGG
GTCAATATGTATTCCTTGGGGCCAAAATTGTAAATAACCTAAAGGATTTGAGAGTAATAT
AAATATGAATGAAGTTGAATGCTCTGTCTTCATTTAGTGTACATTACCATATTAGTGTAC
ATTACTGTATTAAAGGATCTGAGAGATCATGCCAATAGGAAAATGTACTTAACATTTTTA
ACTCAGCATTTTTCAAATATATTTGATCATGGCCCTTCCCTCTGCCCCTCCTCTTATTTC
CTCCCACCCCCAAATAACACCCAACAGCTTCATGTTAATATGTTCTGTTAGGTAAAATGG
ATGGGATGTAAACATTAGGTTTATTTTATGTTTTATATGTATAGTAAATAAAAAATGAAT
GA
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Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
Type(s) of Resistant Mechanism of This Molecule
  EADR: Epigenetic Alteration of DNA, RNA or Protein
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
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Temozolomide
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Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Epigenetic Alteration of DNA, RNA or Protein (EADR) Click to Show/Hide
Disease Class: Brain glioma [ICD-11: 2A00.02] [1]
Resistant Disease Brain glioma [ICD-11: 2A00.02]
 Disease Info 
Resistant Drug Temozolomide
 Drug Info 
Molecule Alteration Expression
Up-regulation
Experimental Note Revealed Based on the Cell Line Data
Cell Pathway Regulation PI3K/AKT pathway Regulation N.A.
In Vitro Model LN229 cells Brain Homo sapiens (Human) CVCL_0393
SHG-44 cells Brain Homo sapiens (Human) CVCL_6728
Experiment for
Molecule Alteration		 RT-qPCR
Experiment for
Drug Resistance		 CCK8 assay
Mechanism Description MSC-AS1 was up-regulated in TMZ-resistant glioma tissues and cells, and glioma patients with high MSC-AS1 expression tend to have lower overall survival rate. MSC-AS1 suppression reduced the IC50 value of TMZ and proliferation, promoted apoptosis and TMZ sensitivity, and affected PI3K/Akt pathway in TMZ-resistant glioma cells. MSC-AS1 acted as miR-373-3p sponge, and miR-373-3p directly targeted CPEB4. Silencing miR-373-3p reversed the promoting effect of MSC-AS1 or CPEB4 knockdown on TMZ sensitivity. Furthermore, MSC-AS1 knockdown inhibited TMZ-resistant glioma growth in vivo by regulating miR-373-3p/CPEB4 axis through PI3K/Akt pathway. Collectively, MSC-AS1 knockdown suppressed cell growth and the chemoresistance of glioma cells to TMZ by regulating miR-373-3p/CPEB4 axis in vitro and in vivo through activating PI3K/Akt pathway.
References
Ref 1 Indian J Med Paediatr Oncol. 2015 Apr-Jun;36(2):133-6. doi: 10.4103/0971-5851.158852.

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