Molecule Information

General Information of the Molecule (ID: Mol04439)

• Name: Guanine nucleotide-binding protein-like 3-like protein (GNL3L) ,Homo sapiens
• Molecule Type: Protein
• Gene Name: GNL3L
• Gene ID: 54552
• Sequence:
  MMKLRHKNKKPGEGSKGHKKISWPYPQPAKQNGKKATSKVPSAPHFVHPNDHANREAELK
  KKWVEEMREKQQAAREQERQKRRTIESYCQDVLRRQEEFEHKEEVLQELNMFPQLDDEA
  T RKAYYKEFRKVVEYSDVILEVLDARDPLGCRCFQMEEAVLRAQGNKKLVLVLNKIDLV
  PK EVVEKWLDYLRNELPTVAFKASTQHQVKNLNRCSVPVDQASESLLKSKACFGAENLM
  RVL GNYCRLGEVRTHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGITKFMQEVYLD
  KFIR LLDAPGIVPGPNSEVGTILRNCVHVQKLADPVTPVETILQRCNLEEISNYYGVSG
  FQTTE HFLTAVAHRLGKKKKGGLYSQEQAAKAVLADWVSGKISFYIPPPATHTLPTHLS
  AEIVKE MTEVFDIEDTEQANEDTMECLATGESDELLGDTDPLEMEIKLLHSPMTKIADA
  IENKTTV YKIGDLTGYCTNPNRHQMGWAKRNVDHRPKSNSMVDVCSVDRRSVLQRIMET
  DPLQQGQA LASALKNKKKMQKRADKIASKLSDSMMSALDLSGNADDGVGD
• Function: Stabilizes TERF1 telomeric association by preventing TERF1recruitment by PML. Stabilizes TERF1 protein by preventing itsubiquitination and hence proteasomal degradation. Does so byinterfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase.Required for cell proliferation. By stabilizing TRF1 protein duringmitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2protein by preventing its ubiquitination, and hence proteasomaldegradation. By acting on MDM2, may affect TP53 activity. Required fornormal processing of ribosomal pre-rRNA. Binds GTP.{ECO:0000269|PubMed:16251348, ECO:0000269|PubMed:17034816,ECO:0000269|PubMed:19487455, ECO:0000269|PubMed:21132010}.
• Uniprot ID: GNL3L_HUMAN
• Ensembl ID: ENSG0000013011917
• HGNC ID: HGNC:25553

Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

  UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Clinical Trial Drug(s) 1 drug(s) in total

Calycosin

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms

Unusual Activation of Pro-survival Pathway (UAPP)

Disease Class: Lung adenocarcinoma [ICD-11: 2C25.0] [1]

• Sensitive Disease: Lung adenocarcinoma [ICD-11: 2C25.0]
• Sensitive Drug: Calycosin
• Molecule Alteration: Expression; Down-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• Cell Pathway Regulation: LDOC1/GNL3L/NFkappaB signalling pathway; Regulation; N.A.
• In Vitro Model: CL1-0 GEMR cells; Lung; Homo sapiens (Human); N.A.
• Experiment for Molecule Alteration: Western blot assay
• Experiment for Drug Resistance: MTT assay; Flow cytometric assay; Colonyformation assay
• Mechanism Description: Our results show that calycosin inhibits the proliferation and migration abilities of CL1-0 GEMR cells, and that these effects might be through the LDOC1/GNL3L/NFkappaB pathway. These findings improve our understanding of the anti-tumor activity and potential mechanisms of calycosin in gemcitabine-resistant lung cancer cells, and offer valuable support for the clinical application of calycosin in treating gemcitabine-resistant lung cancer.

References

• Ref 1: Calycosin inhibits gemcitabine-resistant lung cancer cells proliferation through modulation of the LDOC1/GNL3L/NFkappaB. Chin J Physiol. 2023 Jul-Aug;66(4):189-199.