Molecule Information

General Information of the Molecule (ID: Mol04217)

• Name: Penicillin-binding protein 2C (PBP2C) ,Streptococcus pneumoniae
• Synonyms: Penicillin-binding protein 2C (PBP2C)
• Molecule Type: Protein

Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Lactobacillales
Family: Streptococcaceae
Genus: Streptococcus
Species: Streptococcus pneumoniae

Type(s) of Resistant Mechanism of This Molecule

  ADTT: Aberration of the Drug's Therapeutic Target

Drug Resistance Data Categorized by Drug

Approved Drug(s) 2 drug(s) in total

Cefalotin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

Disease Class: Lactobacillus casei infection [ICD-11: 1A00-1C4Z] [1]

• Resistant Disease: Lactobacillus casei infection [ICD-11: 1A00-1C4Z]
• Resistant Drug: Cefalotin
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: jk0412 cells; N.A.; Homo sapiens (Human); N.A.
• Experiment for Molecule Alteration: Western blot assay
• Experiment for Drug Resistance: Disk diffusion assay; Microdilution assay; E-Test assay
• Mechanism Description: Six genes encoding putative high molecular weight penicillin-binding proteins (Pbp) are present in the genome of the beta-lactam-resistant strain?Corynebacterium jeikeium?K411. In this study, we show that?pbp2c, one of these six genes, is present in resistant strains of?Corynebacteriaceae?but absent from sensitive strains. The molecular study of the?pbp2c?locus from?C. jeikeium?and its heterologous expression in?Corynebacterium glutamicum?allowed us to show that Pbp2c confers high levels of beta-lactam resistance to the host and is under the control of a beta-lactam-induced regulatory system encoded by two adjacent genes,?jk0410?and?jk0411. The detection of this inducible resistance may require up to 48?h of incubation, particularly in?Corynebacterium amycolatum. Finally, the Pbp3c-expressing strains studied were resistant to all the beta-lactam antibiotics tested, including carbapenems, ceftaroline, and ceftobiprole.

Disease Class: Lactobacillus casei infection [ICD-11: 1A00-1C4Z] [1]

• Resistant Disease: Lactobacillus casei infection [ICD-11: 1A00-1C4Z]
• Resistant Drug: Cefalotin
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: cu1571 cells; N.A.; Homo sapiens (Human); N.A.
• Experiment for Molecule Alteration: Western blot assay
• Experiment for Drug Resistance: Disk diffusion assay; Microdilution assay; E-Test assay
• Mechanism Description: Six genes encoding putative high molecular weight penicillin-binding proteins (Pbp) are present in the genome of the beta-lactam-resistant strain?Corynebacterium jeikeium?K411. In this study, we show that?pbp2c, one of these six genes, is present in resistant strains of?Corynebacteriaceae?but absent from sensitive strains. The molecular study of the?pbp2c?locus from?C. jeikeium?and its heterologous expression in?Corynebacterium glutamicum?allowed us to show that Pbp2c confers high levels of beta-lactam resistance to the host and is under the control of a beta-lactam-induced regulatory system encoded by two adjacent genes,?jk0410?and?jk0411. The detection of this inducible resistance may require up to 48?h of incubation, particularly in?Corynebacterium amycolatum. Finally, the Pbp6c-expressing strains studied were resistant to all the beta-lactam antibiotics tested, including carbapenems, ceftaroline, and ceftobiprole.

Penicillin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

Disease Class: Lactobacillus casei infection [ICD-11: 1A00-1C4Z] [1]

• Resistant Disease: Lactobacillus casei infection [ICD-11: 1A00-1C4Z]
• Resistant Drug: Penicillin
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: jk0412 cells; N.A.; Homo sapiens (Human); N.A.
• Experiment for Molecule Alteration: Western blot assay
• Experiment for Drug Resistance: Disk diffusion assay; Microdilution assay; E-Test assay
• Mechanism Description: Six genes encoding putative high molecular weight penicillin-binding proteins (Pbp) are present in the genome of the beta-lactam-resistant strain?Corynebacterium jeikeium?K411. In this study, we show that?pbp2c, one of these six genes, is present in resistant strains of?Corynebacteriaceae?but absent from sensitive strains. The molecular study of the?pbp2c?locus from?C. jeikeium?and its heterologous expression in?Corynebacterium glutamicum?allowed us to show that Pbp2c confers high levels of beta-lactam resistance to the host and is under the control of a beta-lactam-induced regulatory system encoded by two adjacent genes,?jk0410?and?jk0411. The detection of this inducible resistance may require up to 48?h of incubation, particularly in?Corynebacterium amycolatum. Finally, the Pbp2c-expressing strains studied were resistant to all the beta-lactam antibiotics tested, including carbapenems, ceftaroline, and ceftobiprole.

Disease Class: Lactobacillus casei infection [ICD-11: 1A00-1C4Z] [1]

• Resistant Disease: Lactobacillus casei infection [ICD-11: 1A00-1C4Z]
• Resistant Drug: Penicillin
• Molecule Alteration: Expression; Up-regulation
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: cu1571 cells; N.A.; Homo sapiens (Human); N.A.
• Experiment for Molecule Alteration: Western blot assay
• Experiment for Drug Resistance: Disk diffusion assay; Microdilution assay; E-Test assay
• Mechanism Description: Six genes encoding putative high molecular weight penicillin-binding proteins (Pbp) are present in the genome of the beta-lactam-resistant strain?Corynebacterium jeikeium?K411. In this study, we show that?pbp2c, one of these six genes, is present in resistant strains of?Corynebacteriaceae?but absent from sensitive strains. The molecular study of the?pbp2c?locus from?C. jeikeium?and its heterologous expression in?Corynebacterium glutamicum?allowed us to show that Pbp2c confers high levels of beta-lactam resistance to the host and is under the control of a beta-lactam-induced regulatory system encoded by two adjacent genes,?jk0410?and?jk0411. The detection of this inducible resistance may require up to 48?h of incubation, particularly in?Corynebacterium amycolatum. Finally, the Pbp5c-expressing strains studied were resistant to all the beta-lactam antibiotics tested, including carbapenems, ceftaroline, and ceftobiprole.

References

• Ref 1: Exploration of the role of the penicillin binding protein 2c (Pbp2c) in inducible beta-lactam resistance in Corynebacteriaceae. Front Microbiol. 2024 May 9;15:1327723.