Molecule Information

General Information of the Molecule (ID: Mol02020)

• Name: Enoyl-CoA hydratase 2 (HADA) ,Mycobacterium tuberculosis
• Synonyms: hadA; NCGM2209_1021
• Molecule Type: Protein
• Gene Name: HADA
• Gene ID: 45424594
• Sequence:
  MALSADIVGMHYRYPDHYEVEREKIREYAVAVQNDDAWYFEEDGAAELGYKGLLAPLTFI
  CVFGYKAQAAFFKHANIATAEAQIVQVDQVLKFEKPIVAGDKLYCDVYVDSVREAHGTQI
  IVTKNIVTNEEGDLVQETYTTLAGRAGEDGEGFSDGAA
• Uniprot ID: A0A7U9PK65_MYCTX

Kingdom: N.A.
Phylum: Actinobacteria
Class: Actinomycetia
Order: 85007
Family: Mycobacteriaceae
Genus: Mycobacterium
Species: Mycobacterium tuberculosis

Type(s) of Resistant Mechanism of This Molecule

  DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Perchlozone

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Disease Class: Multidrug-resistant tuberculosis [ICD-11: 1B10.2] [1]

• Resistant Disease: Multidrug-resistant tuberculosis [ICD-11: 1B10.2]
• Resistant Drug: Perchlozone
• Molecule Alteration: Frameshift mutation; c.13CGG > CCC (p.Arg > Pro)
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Streptococcus pneumoniae strain; 1313
• Experiment for Molecule Alteration: Whole genome sequencing assay
• Mechanism Description: Perchlozone is a prodrug that is activated by EthA and inhibits the HadABC complex.A resistance to perchlozone was shown by in vitro experiments and was mediated by both ethA and hadA mutations.

References

• Ref 1: Genetic Variation Putatively Associated with Mycobacterium tuberculosis Resistance to Perchlozone, a New Thiosemicarbazone: Clues from Whole Genome Sequencing and Implications for Treatment of Multidrug-Resistant Tuberculosis .Antibiotics (Basel). 2020 Oct 3;9(10):669. doi: 10.3390/antibiotics9100669. 10.3390/antibiotics9100669