Molecule Information

General Information of the Molecule (ID: Mol01304)

Name	AGAP2 antisense RNA 1 (AGAP2-AS1) ,Homo sapiens
Synonyms	AGAP2-AS1 Click to Show/Hide
Molecule Type	LncRNA
Gene Name	LINC00057, NCRNA00057, UHG, lncRNA16
Gene ID	100130776
Location	chr12:57726271-57728356[+]
Ensembl ID	ENSG00000255737
HGNC ID	HGNC:48633
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

RTDM: Regulation by the Disease Microenvironment

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Trastuzumab

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Regulation by the Disease Microenvironment (RTDM)			Click to Show/Hide
Disease Class: Breast cancer				[1]
Resistant Disease	Breast cancer [ICD-11: 2C60.3]			Disease Info
Resistant Drug	Trastuzumab			Drug Info
Molecule Alteration	Expression		Up-regulation
Experimental Note	Identified from the Human Clinical Data
Cell Pathway Regulation	Cell apoptosis		Inhibition	hsa04210
	Cell colony		Activation	hsa05200
	Cell proliferation		Activation	hsa05200
	NF-kappaB signaling pathway		Activation	hsa04064
In Vitro Model	SkBR3 cells	Breast	Homo sapiens (Human)	CVCL_0033
	BT474 cells	Breast	Homo sapiens (Human)	CVCL_0179
In Vivo Model	BALB/c nude mouse xenograft model			Mus musculus
Experiment for Molecule Alteration	RT-qPCR
Experiment for Drug Resistance	MTT assay; TUNEL assay
Mechanism Description	AGAP2-AS1 could promote breast cancer growth and trastuzumab resistance by activating the NF-kB signaling pathway and upregulating MyD88 expression.

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Regulation by the Disease Microenvironment (RTDM)			Click to Show/Hide
Disease Class: Breast cancer				[2]
Sensitive Disease	Breast cancer [ICD-11: 2C60.3]			Disease Info
Sensitive Drug	Trastuzumab			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Revealed Based on the Cell Line Data
Cell Pathway Regulation	Cell apoptosis		Activation	hsa04210
	Cell viability		Inhibition	hsa05200
In Vitro Model	SkBR3 cells	Breast	Homo sapiens (Human)	CVCL_0033
	BT474 cells	Breast	Homo sapiens (Human)	CVCL_0179
Experiment for Molecule Alteration	qRT-PCR
Experiment for Drug Resistance	CCK8 assay; TUNEL assay; Flow cytometry assay
Mechanism Description	Exosomal AGAP2-AS1 expression was upregulated by hnRNPA2B1 overexpression and suppressed by hnRNPA2B1 knockdown in SkBR-3R cells while knockdown of AGAP2-AS1 resensitized trastuzumab resistance in breast cancer cells.

References

Ref 1	SP1-induced lncRNA AGAP2-AS1 expression promotes chemoresistance of breast cancer by epigenetic regulation of MyD88. J Exp Clin Cancer Res. 2018 Aug 29;37(1):202. doi: 10.1186/s13046-018-0875-3.
Ref 2	Increased Expression of Exosomal AGAP2-AS1 (AGAP2 Antisense RNA 1) In Breast Cancer Cells Inhibits Trastuzumab-Induced Cell Cytotoxicity. Med Sci Monit. 2019 Mar 26;25:2211-2220. doi: 10.12659/MSM.915419.