Molecule Information

General Information of the Molecule (ID: Mol01003)
Name
Metallo-beta-lactamase NDM-4 (NDM4) ,Escherichia coli
Synonyms
bla(NDM-4); blaNDM-4; NDM-4; NDM-4; New Delhi metallo-beta-lactamase NDM-4
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Molecule Type
Protein
Gene Name
bla(NDM-4)
Sequence
MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQ
HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTH
AHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTFAANGWVEPATAPNFGPL
KVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAF
PKASMIVMSHSAPDSRAAITHTARMADKLR
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3D-structure
PDB ID
4TYF
Classification
Hydrolase
Method
X-ray diffraction
Resolution
1.10  Å
Uniprot ID
H6WZS9_ECOLX
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Kingdom: N.A.
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Enterobacterales
Family: Enterobacteriaceae
Genus: Escherichia
Species: Escherichia coli
Type(s) of Resistant Mechanism of This Molecule
  DISM: Drug Inactivation by Structure Modification
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
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Ticarcillin
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Drug Resistance Data Categorized by Their Corresponding Mechanisms
  Drug Inactivation by Structure Modification (DISM) Click to Show/Hide
Disease Class: Bacterial infection [ICD-11: 1A00-1C4Z] [1]
Resistant Disease Bacterial infection [ICD-11: 1A00-1C4Z]
 Disease Info 
Resistant Drug Ticarcillin
 Drug Info 
Molecule Alteration Missense mutation
p.M154L
Experimental Note Identified from the Human Clinical Data
In Vitro Model Escherichia coli I5 562
Experiment for
Molecule Alteration		 Whole genome sequence assay; Allelic frequency measurement assay
Experiment for
Drug Resistance		 Disk diffusion test assay; E-strip test assay
Mechanism Description A clinical Escherichia coli isolate resistant to all Beta-lactams, including carbapenems, expressed a novel metallo-Beta-lactamase (MBL), NDM-4, differing from NDM-1 by a single amino acid substitution (Met154Leu). NDM-4 possessed increased hydrolytic activity toward carbapenems and several cephalosporins compared to that of NDM-1.
References
Ref 1 NDM-4 metallo-Beta-lactamase with increased carbapenemase activity from Escherichia coli. Antimicrob Agents Chemother. 2012 Apr;56(4):2184-6. doi: 10.1128/AAC.05961-11. Epub 2012 Jan 17.

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