Molecule Information

General Information of the Molecule (ID: Mol00949)

Name	Elongation factor Tu (TUF) ,Clostridioides difficile
Synonyms	EF-Tu; tufA; CD630_00580; tufB; CD630_00710 Click to Show/Hide
Molecule Type	Protein
Gene Name	tuf1
Gene ID	66352569
Sequence	MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERER GITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREH ILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMAL EDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLK VQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTG SVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNV TMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE Click to Show/Hide
Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Click to Show/Hide
Uniprot ID	EFTU_CLOD6
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Clostridia Order: Eubacteriales Family: Peptostreptococcaceae Genus: Clostridioides Species: Clostridioides difficile

Type(s) of Resistant Mechanism of This Molecule

ADTT: Aberration of the Drug's Therapeutic Target

Drug Resistance Data Categorized by Drug

Clinical Trial Drug(s)

1 drug(s) in total

Click to Show/Hide the Full List of Drugs

LFF571

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Aberration of the Drug's Therapeutic Target (ADTT)		Click to Show/Hide
Disease Class: Bacterial infection			[1]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	LFF571		Drug Info
Molecule Alteration	Missense mutation	p.G260E
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Clostridium difficile strain ATCC 43255		499175
	Clostridium difficile strain NB95002		1496
	Clostridium difficile strain NB95026		1496
	Clostridium difficile strain NB95031		1496
	Clostridium difficile strain NB95047		1496
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	Selection on inhibitory concentrations of LFF571 resulted in a substitution at the C. difficile residue analogous to G257 in E. coli EF-Tu.All mutants exhibited tufB mutation G782A, resulting in amino acid substitution G260E; NB95013-JAL0759 harbored the G782A change in both tufA and tufB.

References

Ref 1	Mechanism of action of and mechanism of reduced susceptibility to the novel anti-Clostridium difficile compound LFF571. Antimicrob Agents Chemother. 2012 Aug;56(8):4463-5. doi: 10.1128/AAC.06354-11. Epub 2012 May 29.