Molecule Information

General Information of the Molecule (ID: Mol00925)

• Name: DNA gyrase subunit B (GYRB) ,Morganella morganii
• Synonyms: H8R20_15075; NCTC235_00006
• Molecule Type: Protein
• Gene Name: gyrB
• Gene ID: 67496344
• Sequence:
  MSNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGYCKDII
  VTIHNDNSVSVQDDGRGIPTGIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
  SVVNALSEKLELVIRRDGKVHEQIYRHGEPQDRLTVVGETDKTGTRVRFWPSMDTFKGET
  EFQYDILAKRLRELSFLNSGVSIRLIDKRDGKEDHFHYEGGIKAFVEYLSRAKTSIHNNV
  FYFSTEKDDIGVEISMQWNDSFQENVYCFTNNIPQRDGGAHLAGFRAAMTRTLNSYIEKE
  GLNKKSKVSTTGDDAREGLVAVISVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLSEY
  LDENPNDTKIIVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPAFSEL
  YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLASQEVATLITALGCGIGR
  DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGYVYIAQPPLYKVKKG
  KQEQYIKDDEAMEQYQVSIALDGAALYVNENAAPIQGEHLEKLLHEYNGAHKIIRRLERL
  YPLALLNSLVYQPKLEESALLNKTEVEAWAQSLTERLTRHEEHGSTYSYRIAENKERQLF
  EPVLTIRTHGVDTDYNLDFDFVHGSEYARISKLGELIRGLIEEGAYVVRGERRQNVSNFE
  QALDWLMKESRRGLAVQRYKGLGEMNPEQLWETTMNPETRRMLQVTVKDAIATDQLFTTL
  MGDDVEPRRAFIEENALKAANIDV
• Function: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
• Uniprot ID: A0A3S4CQ88_MORMO

Kingdom: N.A.
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Enterobacterales
Family: Morganellaceae
Genus: Morganella
Species: Morganella morganii

Type(s) of Resistant Mechanism of This Molecule

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Fluoroquinolones

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Disease Class: Tuberculosis [ICD-11: 1B10.0] [1]

• Resistant Disease: Tuberculosis [ICD-11: 1B10.0]
• Resistant Drug: Fluoroquinolones
• Molecule Alteration: Mutation; R658G
• Experimental Note: Revealed Based on the Cell Line Data
• Experiment for Molecule Alteration: GeneSeq assay; Bioinformatics assay
• Mechanism Description: Out of total 112 mycobacterial positive cultures, five?M. bovis?were isolated and underwent WGS. All sequenced strains belonged to?Mycobacterium tuberculosis var bovis, spoligotype BOV_1; BOV_11. Resistance gene mutations were determined in 100% of strains to pyrazinamide (pncA?and?rpsA), isoniazid (KatG?and?ahpC), ethambutol (embB,?embC,?embR?and?ubiA), streptomycin (rpsl) and fluoroquinolones (gyrA?and?gyrB). Rifampin (rpoB?and?rpoC) and delamanid (fbiC) resistance genes were found in 80% of strains. The major represented virulence classes were the secretion system, cell surface components and regulation system.

References

• Ref 1: Genetic diversities and drug resistance in Mycobacterium bovis isolates from zoonotic tuberculosis using whole genome sequencing. BMC Genomics. 2024 Nov 1;25(1):1024.