Molecule Information

General Information of the Molecule (ID: Mol00802)

• Name: Aminoglycoside phosphotransferase (APH) ,Enterococcus faecium
• Synonyms: aph(2')-Ib; Aminoglycoside phosphotransferase
• Molecule Type: Protein
• Gene Name: aph(2')-Ib
• Sequence:
  MVNLDAEIYEHLNKQIKINELRYLSSGDDSDTFLCNEQYVVKVPKRDSVRISQKREFELY
  RFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKEL
  HSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAV
  LFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGR
  KVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELLQTQAEMFIF
• 3D-structure: PDB ID: 3HAV; Classification: Transferase; Method: X-ray diffraction; Resolution: 2.45 Å
• Uniprot ID: Q9EVD7_ENTFC

Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Lactobacillales
Family: Enterococcaceae
Genus: Enterococcus
Species: Enterococcus faecalis

Type(s) of Resistant Mechanism of This Molecule

  DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Plazomicin

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Disease Class: Enterococcus faecium meningitis [ICD-11: 1D01.2] [1]

• Resistant Disease: Enterococcus faecium meningitis [ICD-11: 1D01.2]
• Resistant Drug: Plazomicin
• Molecule Alteration: Expression; Inherence
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Enterococcus faecium SF11770; 1352
• In Vitro Model: Escherichia coli kHE5-2a; 562
• In Vitro Model: Escherichia coli strain DH10b(pMW119); 316385
• Experiment for Molecule Alteration: PCR
• Mechanism Description: High-level gentamicin resistance (MIC >= 500 ug/ml) in enterococci is predominantly mediated by aac(6')-Ie-aph(2")-Ia, which encodes the bifunctional aminoglycoside-modifying enzyme AAC(6')-APH(2"). Found less commonly is aph(2")-Id, another gene recently reported to be associated with high-level gentamicin resistance in enterococci.

References

• Ref 1: Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium. Antimicrob Agents Chemother. 2000 Oct;44(10):2876-9. doi: 10.1128/AAC.44.10.2876-2879.2000.