Molecule Information

General Information of the Molecule (ID: Mol00536)
Name
Quinone reductase 1 (NQO1) ,Homo sapiens
Synonyms
Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1; DIA4; NMOR1
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Molecule Type
Protein
Gene Name
NQO1
Gene ID
1728
Location
chr16:69706996-69726668[-]
Sequence
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKL
KDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERV
FIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFC
GFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMK
KEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
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3D-structure
PDB ID
5EAI
Classification
Oxidoreductase
Method
X-ray diffraction
Resolution
2.90  Å
Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
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Uniprot ID
NQO1_HUMAN
Ensembl ID
ENSG00000181019
HGNC ID
HGNC:2874
        Click to Show/Hide the Complete Species Lineage
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
Type(s) of Resistant Mechanism of This Molecule with Structure Alteration
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Drug
Clinical Trial Drug(s)
1 drug(s) in total
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Amrubicin
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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
  Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Disease Class: Lung adenocarcinoma [ICD-11: 2C25.0] [1]
Sensitive Disease Lung adenocarcinoma [ICD-11: 2C25.0]
 Disease Info 
Sensitive Drug Amrubicin
 Drug Info 
Molecule Alteration Missense mutation
p.P187S (c.559C>T)
Wild Type Structure Method: X-ray diffraction Resolution: 2.30  Å
PDB: 1QBG
Mutant Type Structure Method: X-ray diffraction Resolution: 2.20  Å
PDB: 4CET
   Download The Information of Sequence       Download The Structure File   
RMSD: 0.58
TM score: 0.99168
Amino acid change:
P187S
 : Wild Type Structure
 : Mutant Type Structure
  Mutation site(s) have been marked in red
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Click to Show/Hide the Structure
Experimental Note Revealed Based on the Cell Line Data
In Vitro Model A549 cells Lung Homo sapiens (Human) CVCL_0023
NCI-H69 cells Lung Homo sapiens (Human) CVCL_1579
NCI-H460 cells Lung Homo sapiens (Human) CVCL_0459
NCI-H23 cells Lung Homo sapiens (Human) CVCL_1547
PC-1 cells Pancreas Homo sapiens (Human) CVCL_S978
PC-10 cells Lung Homo sapiens (Human) CVCL_7088
QG56 cells Lung Homo sapiens (Human) CVCL_6943
RERF-LC-MT cells Lung Homo sapiens (Human) CVCL_A473
Sk-LC-6 cells Lung Homo sapiens (Human) CVCL_5474
PC9 cells Lung Homo sapiens (Human) CVCL_B260
SK-LC-2 cells Lung Homo sapiens (Human) CVCL_5468
SK-LC-17 cells Lung Homo sapiens (Human) CVCL_5466
SK-LC-1 cells Lung Homo sapiens (Human) CVCL_5458
SBC3 cells Bone marrow Homo sapiens (Human) CVCL_1678
RERF-LC-OK cells Central nervous system Homo sapiens (Human) CVCL_3154
RERF-LC-MS cells Lung Homo sapiens (Human) CVCL_1655
PC6 cells Adrenal gland Rattus norvegicus (Rat) CVCL_7091
NCI-H417 cells Lung Homo sapiens (Human) CVCL_1602
NCI-H1688 cells Liver Homo sapiens (Human) CVCL_1487
DMS53 cells Lung Homo sapiens (Human) CVCL_1177
ACC-LC-94 cells Lung Homo sapiens (Human) CVCL_7032
ACC-LC-80 cells Lymph node Homo sapiens (Human) CVCL_7028
ACC-LC-73 cells Lung Homo sapiens (Human) CVCL_7026
ACC-LC-67 cells Lymph node Homo sapiens (Human) CVCL_7024
ACC-LC-5 cells Cervical lymph node Homo sapiens (Human) CVCL_7020
ACC-LC-48 cells Pericardial effusion Homo sapiens (Human) CVCL_7018
ACC-LC-314 cells Lung Homo sapiens (Human) CVCL_7011
ACC-LC-174 cells Lung Homo sapiens (Human) CVCL_7007
ACC-LC-172 cells Pericardial effusion Homo sapiens (Human) CVCL_7005
Experiment for
Molecule Alteration		 Western blot analysis
Experiment for
Drug Resistance		 CellTiter 96 AQueous One assay
Mechanism Description The missense mutation p.P187S (c.559C>T) in gene NQO1 cause the sensitivity of Amrubicin by unusual activation of pro-survival pathway
References
Ref 1 C609T polymorphism of NAD(P)H quinone oxidoreductase 1 as a predictive biomarker for response to amrubicinJ Thorac Oncol. 2011 Nov;6(11):1826-32. doi: 10.1097/JTO.0b013e318229137d.

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