HIV Detail Information

Zidovudine (ID: DG00024) Resistance Data of HIV

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

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Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.E44D+p.V60I+p.D67N+p.G93GR+p.K101H+p.V118I+p.K122P+p.D123E+p.I135T+p.I142V+p.D177E+p.I178IM+p.M184V+p.G190A+p.G196E+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[1]
p.P4T+p.E6D+p.K20R+p.A33G+p.V60I+p.D67N+p.T69N+p.K70R+p.L74I+p.K101E+p.K103S+p.V118I+p.D123E+p.I135T+p.E138A+p.M184V+p.G190A+p.D192N+p.I195KR+p.G196E+p.E203K+p.R211A+p.F214L+p.K219Q+p.H221Y+p.I257L+p.A272P+p.K281R+p.E291D+p.E297K	Missense mutation	31	Intermediate resistance	[1]
p.K20R+p.V35L+p.M41L+p.K43E+p.D67N+p.T69N+p.K70R+p.K102Q+p.K103N+p.K122E+p.D123E+p.D177N+p.M184V+p.Y188L+p.G196E+p.I202V+p.R211T+p.T215F+p.D218E+p.K219Q+p.A272P+p.K277R+p.L283I+p.A288T+p.I293V+p.E297K+p.A304E	Missense mutation	60	High-level resistance	[1]
p.P4T+p.E6K+p.K20R+p.T39TK+p.M41L+p.K43E+p.E44AD+p.D67N+p.L74I+p.K101E+p.V118I+p.K122E+p.S162A+p.V179F+p.Y181C+p.M184V+p.G190A+p.I195K+p.L210W+p.R211K+p.T215Y+p.K219N+p.V245K+p.I293V+p.E297A	Missense mutation	12	Potential low-level resistance	[1]
p.E6D+p.M41L+p.E44D+p.K49R+p.V60I+p.D67N+p.K70R+p.K103N+p.V108VI+p.D123E+p.I135T+p.M184V+p.T200I+p.I202V+p.T215V+p.K219E+p.P225PH+p.L228H+p.V245T+p.E248K+p.K249KR+p.I293V+p.E297A	Missense mutation	39	Intermediate resistance	[1]
p.V35I+p.R83K+p.K122KE+p.K173KQ+p.N175H+p.Y188YH+p.T200A+p.R211K+p.S251SN+p.A272P+p.K277R+p.I293IV+p.E297K	Missense mutation	100	High-level resistance	[1]
p.I2V+p.K11KQ+p.V35M+p.T39A+p.M41L+p.K43AE+p.E44D+p.D67N+p.L74I+p.K101E+p.V106M+p.V118I+p.K122E+p.D123DN+p.T139TI+p.M184V+p.G190A+p.D192DN+p.G196E+p.E203K+p.Q207DE+p.H208F+p.L210W+p.R211A+p.T215Y+p.K219R+p.K223E+p.F227L+p.L228H+p.T286A+p.A288S+p.I293V	Missense mutation	100	High-level resistance	[1]
p.E6EK+p.K20R+p.V35L+p.T39A+p.M41L+p.K43E+p.K64KR+p.D67N+p.T69D+p.V118I+p.K122E+p.M184V+p.I195K+p.G196E+p.E203K+p.Q207A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K223Q+p.L228Q+p.V245M+p.T286A+p.I293V+p.E297K	Missense mutation	13	Potential low-level resistance	[1]
p.K20R+p.T39A+p.M41L+p.K43KE+p.K103N+p.V118I+p.K122KE+p.I135IT+p.I142V+p.A158AS+p.S162C+p.T165I+p.V179VI+p.G196E+p.L210W+p.R211RK+p.T215Y+p.P217PS+p.L228R+p.D250E+p.A272P+p.T286A+p.E297R	Missense mutation	88	High-level resistance	[1]
p.T39TS+p.M41L+p.K43E+p.A62V+p.D123DE+p.V179I+p.M184V+p.Q197P+p.R211T+p.T215Y+p.L228R+p.W266A+p.A272P+p.K281R+p.E291ED+p.V292I+p.E297K	Missense mutation	16	Low-level resistance	[1]
p.E6K+p.M41L+p.K43E+p.E44D+p.D67N+p.A98G+p.K103N+p.V111I+p.V118I+p.K122E+p.M184V+p.G196GE+p.T200A+p.E203DH+p.Q207NS+p.H208Y+p.L210W+p.R211K+p.T215Y+p.L228H+p.A272P	Missense mutation	100	High-level resistance	[1]
p.K43N+p.A62V+p.D67E+p.T69S_SS+p.A98AG+p.K122E+p.S162C+p.E169D+p.V179VI+p.G196E+p.Q207E+p.L210W+p.T215Y+p.L228R+p.D237DE+p.K277R+p.I293V+p.E297A	Missense mutation	100	High-level resistance	[1]
p.V35I+p.S68G+p.T69SEG+p.W88C+p.K101P+p.K122E+p.I135V+p.S162C+p.Q174R+p.D177E+p.I178L+p.T215Y+p.L228H+p.M230L+p.V245M+p.A272P+p.A288S+p.I293V+p.E297T	Missense mutation	100	High-level resistance	[1]
p.K20R+p.M41L+p.D67N+p.T69TN+p.K70R+p.L74I+p.V75T+p.A98S+p.K103N+p.V118I+p.K122E+p.I142V+p.S162A+p.Q174HN+p.Y181C+p.T200A+p.F214FL+p.T215F+p.D218E+p.K219Q+p.L228H+p.V245K+p.D250E+p.A272S+p.K281R+p.T286A+p.I293V	Missense mutation	63	High-level resistance	[1]
p.T39AE+p.M41L+p.K43KR+p.E44D+p.L74LI+p.A98AG+p.K102KR+p.K103N+p.K122E+p.D123S+p.I135T+p.I142V+p.M184V+p.Y188L+p.H208Y+p.L210W+p.R211K+p.T215Y+p.L228H+p.R284K+p.T286A+p.P294T	Missense mutation	77	High-level resistance	[1]
p.S3C+p.E28R+p.K32E+p.V35M+p.M41L+p.L74I+p.R83K+p.A98G+p.K101E+p.K103N+p.V108I+p.S162A+p.I167IV+p.E169D+p.D177E+p.I178L+p.M184V+p.G196E+p.T200A+p.E203D+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H+p.L260V+p.A272P+p.K281R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[1]
p.E28K+p.K32E+p.T39K+p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.V75M+p.R83K+p.K102KR+p.K103S+p.V118I+p.K122E+p.I135T+p.V179T+p.Y181V+p.M184V+p.T200A+p.E203V+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219E+p.L228H+p.Q242H+p.V245E+p.D256E+p.A272P+p.T286TI+p.A288T+p.I293V+p.E297T	Missense mutation	100	High-level resistance	[1]
p.K20KR+p.M41L+p.K43KE+p.D67DN+p.E138EG+p.I142IT+p.M184V+p.G190A+p.L210W+p.R211K+p.T215Y+p.K238KNT+p.A272AP+p.K281R+p.R284RK+p.E297K	Missense mutation	31	Intermediate resistance	[1]
p.K20R+p.M41L+p.K66KR+p.D67N+p.T69TN+p.K70R+p.L74I+p.V75T+p.A98S+p.K103N+p.V118I+p.K122E+p.I142V+p.S162A+p.Q174HN+p.Y181C+p.T200A+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228H+p.V245K+p.D250E+p.A272S+p.K281R+p.T286AV+p.I293V	Missense mutation	57	Intermediate resistance	[1]
p.K64R+p.D67G+p.T69DN+p.K70R+p.A98G+p.I142V+p.M184V+p.Y188L+p.G196E+p.R211A+p.F214L+p.K219Q+p.V245T+p.K275R+p.Q278E+p.I293V+p.E297K	Missense mutation	18	Low-level resistance	[1]
p.V35L+p.D67N+p.K70R+p.K101R+p.K103R+p.D177E+p.I178M+p.G190E+p.G196E+p.T200A+p.E204N+p.R211K+p.T215F+p.K219E+p.L228H+p.V245E+p.A272P+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[1]
p.K20KR+p.V35M+p.T39A+p.E40F+p.M41L+p.K43E+p.S68G+p.L74I+p.V75A+p.K103G+p.V108I+p.L109I+p.V118I+p.K122E+p.V179I+p.Y181C+p.M184V+p.G190A+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228H+p.A272P	Missense mutation	16	Low-level resistance	[1]
p.M41L+p.M184V+p.L210W+p.T215Y	Missense mutation	26	Low-level resistance	[1]
p.V21VI+p.D67N+p.T69S+p.K70R+p.I135V+p.N137NH+p.K173E+p.K219Q+p.P243S+p.V245K	Missense mutation	15	Low-level resistance	[1]
p.M41L+p.L74I+p.K102GR+p.K103N+p.K104R+p.V108I+p.K122E+p.I142V+p.K173N+p.Q174K+p.M184V+p.T200A+p.Q207E+p.L210W+p.R211K+p.T215Y+p.H221HY+p.K238KT+p.D250E+p.A272P	Missense mutation	15	Low-level resistance	[1]
p.M41L+p.K104R+p.D123E+p.I135V+p.I142V+p.K166R+p.I178IM+p.M184V+p.T200A+p.T215Y+p.V245E+p.A272P+p.V276VI+p.T286A	Missense mutation	11	Potential low-level resistance	[1]
p.E6DN+p.M41L+p.A98S+p.K103NS+p.K122E+p.D123N+p.Q174QK+p.I178IV+p.T200IK+p.Q207E+p.L210W+p.R211K+p.T215Y+p.V245E+p.T286A+p.E297Q	Missense mutation	100	High-level resistance	[1]
p.I31IR+p.E40K+p.K43E+p.K46Q+p.K49R+p.I50IV+p.T58N+p.A62V+p.S68G+p.T69TI+p.K70N+p.V75I+p.F77L+p.K103NS+p.Y115F+p.F116Y+p.D121DH+p.K122E+p.Q151M+p.P170S+p.Q174K+p.Y181C+p.M184V+p.Y188L+p.T200A+p.Q207E+p.R211K+p.K219T+p.L228H+p.V245T+p.E248D+p.D250E+p.S251D+p.A272P+p.T286A+p.A288S+p.V292I+p.E297K	Missense mutation	100	High-level resistance	[1]
p.K32N+p.M41L+p.A62V+p.S68G+p.T69A_VG+p.V106I+p.S162A+p.Y188C+p.L210W+p.R211K+p.T215Y+p.V245T+p.K277R+p.Q278H+p.R284K+p.T286A	Missense mutation	100	High-level resistance	[1]
p.A33G+p.M41L+p.D67N+p.T69D+p.K70R+p.A98G+p.K103S+p.V118I+p.M184V+p.G190A+p.R211K+p.F214L+p.T215F+p.K219Q+p.L228X+p.A272P+p.I293V+p.E297EA	Missense mutation	100	High-level resistance	[1]
p.D67N+p.K70R+p.L74IV+p.L100I+p.K103N+p.D123E+p.I135T+p.K166R+p.G196E+p.T200A+p.I202V+p.T215I+p.K219E+p.L228LR+p.K277R+p.L283I+p.A288T+p.I293V+p.P294I+p.E297P	Missense mutation	18	Low-level resistance	[1]
p.M41L+p.S48T+p.K103N+p.K122E+p.I135V+p.M184V+p.T200A+p.L210W+p.T215Y+p.E248N+p.D250X+p.A272P+p.I293V	Missense mutation	18	Low-level resistance	[1]
p.P4S+p.A62V+p.K65R+p.S68G+p.V75I+p.F77L+p.W88WS+p.F116Y+p.V118I+p.K122E+p.Q151M+p.V245I+p.E248D+p.V276T+p.Q278H+p.K281R+p.L283I+p.T286TA+p.I293V	Missense mutation	100	High-level resistance	[1]
p.K20R+p.V35K+p.T39A+p.M41L+p.K49KQ+p.D67N+p.T69N+p.K70R+p.W88C+p.A98S+p.K122E+p.D123E+p.I135IT+p.V179I+p.M184V+p.G196E+p.T200A+p.E203K+p.Q207E+p.L210W+p.R211RK+p.F214L+p.T215Y+p.K219E+p.L228H+p.V245M+p.A272S+p.K277R+p.A288S+p.E297K	Missense mutation	19	Low-level resistance	[1]
p.E6D+p.M41L+p.K43E+p.E44ED+p.K49R+p.V60I+p.D67DN+p.S68SG+p.V118I+p.I135T+p.S162D+p.M184V+p.G196E+p.T200IR+p.E203EK+p.Q207DE+p.L210W+p.R211K+p.T215Y+p.L228H+p.A272P+p.I293IV+p.E297R	Missense mutation	73	High-level resistance	[1]
p.M41L+p.K43KQ+p.V60I+p.D67N+p.T69N+p.K70R+p.K101E+p.K122E+p.D123N+p.I135V+p.D177E+p.Y181C+p.G190A+p.T215F+p.D218E+p.K219Q+p.L228HR+p.K281R+p.T286TA+p.A288AS+p.I293V	Missense mutation	100	High-level resistance	[1]
p.K11KT+p.V35M+p.M41L+p.K43N+p.E44D+p.V75M+p.A98G+p.K101E+p.V118I+p.D123E+p.D177E+p.Y181C+p.M184V+p.G190S+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228H+p.A272P	Missense mutation	20	Low-level resistance	[1]
p.V35I+p.T39A+p.M41L+p.E44D+p.D67N+p.K70R+p.V75VA+p.A98AS+p.L109I+p.V118I+p.K122E+p.V189VI+p.I202V+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219E+p.H221HY+p.F227L+p.L228H+p.I257L+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[1]
p.T39A+p.M41L+p.E44D+p.D67DN+p.K103N+p.V118I+p.I142V+p.K173E+p.M184V+p.G190GA+p.Q207E+p.L210W+p.R211K+p.T215Y+p.P225PH+p.A272P+p.Q278H+p.K281R+p.I293V	Missense mutation	90	High-level resistance	[1]
p.K20R+p.T39A+p.M41L+p.V60I+p.D67G+p.S68G+p.T69E+p.K70R+p.A98G+p.K103N+p.K122E+p.D123N+p.I135T+p.Q174QR+p.D177EG+p.I178M+p.V179I+p.M184V+p.T200A+p.T215F+p.D218E+p.K219Q+p.V245K+p.A272P+p.A288S+p.E297K	Missense mutation	100	High-level resistance	[1]
p.T39A+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.V179I+p.M184V+p.G196K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q+p.L228H+p.E248D+p.S251N+p.A272AP+p.T286A	Missense mutation	14	Potential low-level resistance	[1]
p.V35I+p.M41L+p.K43N+p.R83K+p.K103N+p.K122E+p.M184V+p.G196E+p.T200A+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228R+p.V245E+p.Q269H+p.A272P+p.K277R+p.Q278H+p.T286A+p.E297R	Missense mutation	15	Low-level resistance	[1]
p.P1L+p.E28EA+p.E53EG+p.V60VI+p.D67N+p.T69D+p.K70R+p.A98S+p.K101E+p.D123E+p.I135T+p.S162Y+p.V179VI+p.I195T+p.T200A+p.T215F+p.K219Q+p.V245K+p.A272P+p.A288S+p.I293IV	Missense mutation	24	Low-level resistance	[1]
p.K20R+p.V35IM+p.T39A+p.V60I+p.K64H+p.D67N+p.T69N+p.K70R+p.L74V+p.K103N+p.S105A+p.V108I+p.I135T+p.E138A+p.I202V+p.F214L+p.T215TI+p.K219Q+p.H221Y+p.L228H+p.K238T+p.T286A+p.I293V	Missense mutation	11	Potential low-level resistance	[1]
p.M41L+p.S68T+p.T69S_SA+p.K103N+p.D123E+p.Y181C+p.G196E+p.L210W+p.R211K+p.T215Y+p.L228R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[1]
p.E28K+p.S68G+p.A98S+p.K101E+p.D123E+p.I135V+p.I142IT+p.R143RG+p.M184V+p.L210LW+p.R211K+p.T215Y+p.T286TA+p.E297A	Missense mutation	16	Low-level resistance	[1]
p.K13N+p.V35M+p.M41L+p.K43Q+p.E44D+p.D67N+p.D86N+p.K103N+p.V118I+p.Y127YC+p.E138Q+p.S162D+p.M184V+p.G196D+p.L210W+p.R211K+p.T215Y+p.Q278H+p.T286A+p.I293V+p.E297R+p.N348I+p.K366R+p.S379G+p.K385KR+p.T386I+p.A400T+p.G436E+p.N447NS+p.D460N+p.V467I+p.L491S+p.S519ST+p.Q524E+p.A554T	Missense mutation	54	Intermediate resistance	[1]
p.M41L+p.K43Q+p.V60I+p.D67N+p.V90I+p.K104R+p.V118I+p.G196E+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219R+p.V292I+p.I293V	Missense mutation	100	High-level resistance	[1]
p.T7TA+p.K11R+p.V21I+p.M41L+p.K43E+p.E44A+p.T69D+p.L74I+p.R83K+p.K101Q+p.V118I+p.K122E+p.R172K+p.D177E+p.V179I+p.Y181C+p.G190S+p.G196E+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R+p.L228R+p.T286P+p.A288S+p.I293V+p.E297R	Missense mutation	100	High-level resistance	[1]
p.E6D+p.T39A+p.M41L+p.E44D+p.D67N+p.K122E+p.I142V+p.M184V+p.I202V+p.L210W+p.R211K+p.T215Y+p.K219Q+p.T286A+p.A288T	Missense mutation	28	Low-level resistance	[1]
p.K20R+p.T39E+p.M41L+p.E44D+p.D67N+p.T69D+p.L74I+p.A98G+p.K101E+p.V118I+p.K122E+p.I135T+p.Y181C+p.G190A+p.G196E+p.E203K+p.L210W+p.R211K+p.T215Y+p.A272S+p.V276T+p.K277R+p.Q278E+p.L283I+p.I293V+p.E297KR+p.E302R+p.L303T	Missense mutation	100	High-level resistance	[1]
p.K20R+p.D67N+p.K70R+p.K122E+p.I135V+p.E138A+p.S162C+p.M184MV+p.G190E+p.T200A+p.F214M+p.T215F+p.K219Q+p.L228H+p.V245E	Missense mutation	41	Intermediate resistance	[1]
p.K11KT+p.M41L+p.K43N+p.E44D+p.V75M+p.K101E+p.V118I+p.D123E+p.D177E+p.Y181C+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228LR+p.A272P+p.I293V	Missense mutation	100	High-level resistance	[1]
p.E6D+p.K20R+p.T39A+p.M41L+p.D67DN+p.K122E+p.I142V+p.M184V+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.T286A+p.I293V+p.T296S+p.E297K	Missense mutation	33	Intermediate resistance	[1]
p.T7TA+p.K20KR+p.T39TA+p.M41L+p.D67G+p.S68G+p.T69D+p.K70R+p.I135T+p.S162Y+p.Q174QH+p.I178M+p.M184V+p.Q207E+p.T215F+p.L228LHQ+p.A272P+p.A288S+p.E297K	Missense mutation	18	Low-level resistance	[1]
p.E6D+p.M41L+p.K43E+p.L74V+p.V118I+p.Q174H+p.G190Q+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228HR+p.A272P+p.E297K	Missense mutation	57	Intermediate resistance	[1]
p.T39TA+p.D67N+p.K70R+p.V90I+p.K101E+p.S162N+p.M184V+p.G190A+p.E203D+p.R211K+p.F214FL+p.K219H+p.E297A	Missense mutation	24	Low-level resistance	[1]
p.K20R+p.M41L+p.K43N+p.K122E+p.D123NS+p.I142V+p.M184V+p.T200TI+p.L210LW+p.T215Y+p.V245K+p.S251I+p.K275KN	Missense mutation	100	High-level resistance	[1]
p.M41L+p.K101H+p.I135T+p.M184V+p.G190A+p.L210W+p.R211K+p.T215Y+p.E248V+p.A272P+p.K277R+p.Q278QL	Missense mutation	69	High-level resistance	[1]
p.V60I+p.K64Y+p.D67N+p.K70R+p.V90VI+p.K101P+p.T139M+p.M184V+p.G190A+p.K219Q+p.E224D+p.L228H+p.V245E	Missense mutation	31	Intermediate resistance	[1]
p.V35I+p.M41L+p.V60I+p.R83K+p.K104R+p.V118I+p.L210W+p.R211K+p.T215Y+p.S268SG+p.A272S+p.T286A+p.I293V+p.E297A	Missense mutation	13	Potential low-level resistance	[1]
p.I31L+p.M41L+p.T58N+p.K65R+p.D67N+p.S68G+p.T69TI+p.V75I+p.F77L+p.V90I+p.K101Q+p.Y115F+p.F116Y+p.D123E+p.Q151M+p.K166R+p.D177E+p.Y181C+p.M184I+p.G190A+p.T200I+p.I202V+p.R211K+p.K219Q+p.H221Y+p.V245T+p.D250E+p.A272P+p.K277R+p.I293V	Missense mutation	100	High-level resistance	[1]
p.M41L+p.E44D+p.D67N+p.V118VI+p.I135T+p.S162C+p.R172K+p.Y188L+p.V189I+p.T200A+p.L210W+p.T215Y+p.D218E+p.K219R+p.V245E+p.A272P	Missense mutation	100	High-level resistance	[1]
p.M41L+p.V60I+p.D67N+p.K101E+p.I135T+p.E138A+p.M184V+p.R211K+p.T215F+p.D218E+p.K219Q+p.K277KR+p.T286A+p.E297K	Missense mutation	18	Low-level resistance	[1]
p.M41L+p.K43Q+p.I50V+p.V60I+p.D67N+p.D123E+p.D177E+p.G196E+p.T200R+p.L210W+p.R211K+p.T215Y+p.A272P+p.I293V	Missense mutation	100	High-level resistance	[1]
p.S3SR+p.V35I+p.M41L+p.K43E+p.V60I+p.D67N+p.V75M+p.K101E+p.V118I+p.D121Y+p.K122E+p.Q174K+p.D177E+p.I178M+p.G190S+p.T200A+p.Q207A+p.L210W+p.R211K+p.T215Y+p.A272P+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[1]
p.A62V+p.D67E+p.T69S_SS+p.K122E+p.I142V+p.D177E+p.G190S+p.T215Y+p.L228H+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[1]
p.E40F+p.M41L+p.K43E+p.D67N+p.L74I+p.K101H+p.V118I+p.K122E+p.S162C+p.E169D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.V245M+p.Q278QH+p.I293V	Missense mutation	100	High-level resistance	[1]
p.T39A+p.M41L+p.S68G+p.I135T+p.K166R+p.Y181V+p.T200A+p.L210W+p.R211K+p.T215Y+p.Q278H+p.T286A+p.A288S+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[1]
p.T39A+p.M41L+p.K43E+p.E44A+p.D67N+p.L74V+p.K101KR+p.K103N+p.V111I+p.V118I+p.K122KE+p.I135T+p.D177DE+p.V179F+p.Y181C+p.G190A+p.Q207QE+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228HR+p.V245M+p.A272P+p.K277R	Missense mutation	25	Low-level resistance	[1]
p.K20R+p.T39A+p.M41L+p.K43E+p.E44EA+p.D67N+p.L74V+p.K103N+p.K122P+p.Q174R+p.Y181C+p.G190A+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.A288S+p.I293V+p.P294PA+p.E297K+p.A304G	Missense mutation	69	High-level resistance	[1]
p.V35I+p.M41L+p.V60I+p.D67N+p.V106A+p.V118I+p.K122E+p.I135V+p.I195T+p.L210W+p.T215Y+p.K219R+p.K223KT+p.F227L+p.V245K+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[1]
p.M16ML+p.V35I+p.M41L+p.K43N+p.V60I+p.D67N+p.T69N+p.A98S+p.K103N+p.F116Y+p.K122E+p.I135V+p.Q151M+p.I167V+p.Y181C+p.G196E+p.T200A+p.E203K+p.Q207D+p.H208Y+p.T215F+p.K219Q+p.L228H+p.V245E+p.K263KN+p.S268CR+p.L279LF+p.T286TA+p.I293V	Missense mutation	39	Intermediate resistance	[1]
p.P1F+p.M41L+p.D67N+p.K70R+p.K103N+p.D123E+p.I135L+p.K166R+p.D177E+p.G196E+p.T215F+p.K238KT+p.V245M+p.K277R+p.T286A+p.I293V+p.E297K+p.A299AG	Missense mutation	100	High-level resistance	[1]
p.V35T+p.T39A+p.M41L+p.E44ED+p.V60I+p.D67N+p.T69D+p.V75M+p.F77L+p.V118I+p.K122E+p.I135T+p.E138K+p.S162X+p.M184V+p.H208HY+p.L210W+p.R211RK+p.T215Y+p.A272P+p.K277R+p.T286A	Missense mutation	100	High-level resistance	[1]
p.K20R+p.M41L+p.K49KR+p.K103KN+p.K122E+p.K126KR+p.I135IL+p.N137S+p.E138E*+p.D177G+p.Q207E+p.R211K+p.T215Y+p.A272P+p.Q278QH+p.T286TA	Missense mutation	43	Intermediate resistance	[1]
p.M41L+p.V60I+p.D67N+p.K70R+p.V75VM+p.K122E+p.I135V+p.R199K+p.R211K+p.T215F+p.K219Q+p.I293V	Missense mutation	95	High-level resistance	[1]
p.M41L+p.K43KE+p.D67N+p.A98S+p.V118I+p.I135V+p.S162C+p.T200A+p.Q207E+p.T215Y+p.V245E+p.P247PA+p.S251I+p.I293V	Missense mutation	71	High-level resistance	[1]
p.D67N+p.T69D+p.K70R+p.A98S+p.K101E+p.D123E+p.I135T+p.S162X+p.M184V+p.I195T+p.T200A+p.T215F+p.K219Q+p.V245K+p.A272P+p.A288AS	Missense mutation	18	Low-level resistance	[1]
p.K20R+p.V35M+p.M41L+p.D67N+p.T69D+p.K103N+p.K122E+p.D123E+p.T200A+p.Q207N+p.L210W+p.R211K+p.T215Y+p.A304G	Missense mutation	100	High-level resistance	[1]
p.K32KE+p.M41L+p.D67DN+p.V118VI+p.D121X+p.K122E+p.I135V+p.K166R+p.D177E+p.M184V+p.L210W+p.R211K+p.T215Y+p.V245M+p.A272P+p.K277R+p.I293V	Missense mutation	16	Low-level resistance	[1]
p.V35I+p.M41L+p.E44D+p.V60I+p.D67N+p.T69D+p.R83K+p.A98G+p.V118I+p.K122E+p.D123N+p.I135M+p.D177E+p.I178L+p.M184V+p.L210W+p.R211K+p.T215Y+p.V245E+p.A272P+p.K277R+p.I293V+p.L295LP+p.E297A+p.E300EV	Missense mutation	76	High-level resistance	[1]
p.V21I+p.V35I+p.M41L+p.E44D+p.D67DN+p.T69D+p.R83K+p.K122E+p.M184V+p.T200A+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.L228H+p.A272P+p.K281R+p.I293V+p.E297Q	Missense mutation	50	Intermediate resistance	[1]
p.M41L+p.K43Q+p.D67N+p.A98G+p.K103N+p.K122E+p.I135V+p.Q174H+p.G196E+p.L210W+p.T215Y+p.A272P+p.K277R+p.I293V	Missense mutation	100	High-level resistance	[1]
p.K20R+p.M41L+p.V60I+p.D67N+p.T69N+p.K70R+p.L74V+p.K122E+p.D123DN+p.I135T+p.Y181C+p.G190A+p.G196E+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.L228LH	Missense mutation	86	High-level resistance	[1]
p.M41L+p.D67N+p.T69D+p.K70R+p.K122E+p.I135IV+p.I142V+p.M184V+p.G196E+p.T215F+p.V245MT+p.I293V	Missense mutation	12	Potential low-level resistance	[1]
p.K64N+p.D67N+p.K70R+p.A98S+p.D123E+p.T131TP+p.I135T+p.D177E+p.Q197E+p.Q207H+p.R211K+p.K219Q+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	42	Intermediate resistance	[1]
p.V35M+p.M41L+p.D67N+p.R83K+p.V106A+p.D123E+p.S162A+p.Q207E+p.T215Y+p.F227L+p.D237E+p.V245Q	Missense mutation	24	Low-level resistance	[1]
p.V21I+p.V35M+p.M41L+p.K43N+p.E44D+p.D67N+p.T69D+p.V106I+p.V118I+p.K122E+p.D123E+p.I135T+p.I178M+p.Q197I+p.H208Y+p.L210W+p.R211K+p.T215Y	Missense mutation	100	High-level resistance	[1]
p.M41L+p.E44D+p.D67N+p.T69D+p.K70KR+p.A98S+p.Y181I+p.L210W+p.R211K+p.T215Y+p.D218DE	Missense mutation	100	High-level resistance	[1]
p.K11R+p.K20R+p.M41L+p.K43DE+p.E44A+p.V60I+p.D67N+p.K103N+p.V118I+p.D121Y+p.K122E+p.I135T+p.Y181C+p.G196GE+p.T200I+p.L210W+p.R211K+p.T215Y+p.K219N+p.A272P+p.K277R+p.I293V	Missense mutation	100	High-level resistance	[1]
p.T39A+p.E40F+p.M41L+p.K43E+p.V60I+p.D67N+p.V75M+p.K101P+p.K103N+p.K122E+p.D123E+p.I135T+p.I178M+p.V179I+p.M184V+p.G196E+p.E203K+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219Q+p.L228H+p.A272P+p.T286A	Missense mutation	100	High-level resistance	[1]
p.V35L+p.M41L+p.A62V+p.K64KR+p.D67N+p.L74I+p.K103N+p.K122E+p.K173E+p.I178L+p.V179VI+p.M184V+p.L210W+p.T215F+p.D218E+p.K219Q+p.L228H	Missense mutation	77	High-level resistance	[1]
p.F77L+p.F116Y+p.Q151M+p.G196E+p.V245M+p.N265NT+p.I293V	Missense mutation	40	Intermediate resistance	[1]
p.K20R+p.M41L+p.K43E+p.D67N+p.K122E+p.D123N+p.I178L+p.E203K+p.L210W+p.R211RK+p.T215Y+p.V245M	Missense mutation	100	High-level resistance	[1]
p.E28EK+p.V35I+p.D67N+p.K70R+p.R83K+p.T200A+p.E203K+p.Q207E+p.A272P+p.K277R+p.I293V	Missense mutation	28	Low-level resistance	[1]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Site-directed mutagenesis; Sanger sequencing assay

Experiment for
Drug Resistance

PhenoSense assay

Mechanism Description

Sixteen mutations at eight positions had a mean regression coefficient >=1.0 log10 (a contribution to decreased susceptibility of 10-fold or greater) for nevirapine, efavirenz, etravirine and/or rilpivirine: L100I, k101P, k103N/S, V106A/M, Y181C/I/V, Y188C/L, G190A/E/Q/S and F227C. With the exception of L100I and F227C, each mutation had a mean regression coefficient >=1.0 log10 for nevirapine. With the exception of k103S, V106A, Y181C/I/V and F227C, each had a mean regression coefficient >=1.0 log10 for efavirenz. Five mutations, k101P, Y181I/V, G190E and F227C, had a mean regression coefficient >=1.0 log10 for etravirine. Six mutations, L100I, k101P, Y181I/V, G190E and F227C, had a mean regression coefficient >= 1.0 log10 for rilpivirine.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.K65R+p.D67N+p.S68G+p.V75I+p.F77L+p.Y115F+p.F116Y+p.V118I+p.D123E+p.N147NH+p.Q151M+p.T165TI+p.F171FC+p.D177E+p.I178IV+p.Y181I+p.T200Q+p.Q207H+p.R211K+p.F214L+p.K219E+p.I244IM	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, K65R, D67N, S68G, V75I, F77L, Y115F, F116Y, V118I, D123E, N147NH, Q151M, T165TI, F171FC, D177E, I178IV, Y181I, T200Q, Q207H, R211K, F214L, K219E, I244IM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.K65R+p.S68K+p.K70T+p.V75I+p.F77L+p.K103N+p.V111I+p.Y115F+p.F116Y+p.I132IK+p.I135L+p.E138G+p.Q151M+p.S162H+p.I178IM+p.M184V+p.G196E+p.T200A+p.R211G+p.P225H	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, K65R, S68K, K70T, V75I, F77L, K103N, V111I, Y115F, F116Y, I132IK, I135L, E138G, Q151M, S162H, I178IM, M184V, G196E, T200A, R211G, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38C*+p.D67N+p.K70KR+p.R72RG+p.K101E+p.V111VL+p.K122P+p.D123E+p.R172RK+p.D177E+p.G190GS+p.R211K+p.T215TI+p.K219Q	Missense mutation	31	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38C*, D67N, K70KR, R72RG, K101E, V111VL, K122P, D123E, R172RK, D177E, G190GS, R211K, T215TI, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38C*+p.K43KE+p.K64KR+p.T69G_SG+p.V75M+p.H96HN+p.K103N+p.K122E+p.I135T+p.M184V+p.R211K+p.T215F+p.L228H	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38C*, K43KE, K64KR, T69G_SG, V75M, H96HN, K103N, K122E, I135T, M184V, R211K, T215F, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CF+p.T39TS+p.E40ED+p.V60VI+p.D67N+p.K70R+p.R83K+p.R143RI+p.Q207QK+p.R211RK+p.K219Q	Missense mutation	14	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CF, T39TS, E40ED, V60VI, D67N, K70R, R83K, R143RI, Q207QK, R211RK, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CF+p.T39TS+p.M41L+p.K43Q+p.E44D+p.D67N+p.V75A+p.K102Q+p.D113DN+p.V118I+p.K122E+p.D123E+p.I135IT+p.S162Y+p.D177E+p.M184V+p.G196E+p.Q197QP+p.T200ADE+p.E203K+p.H208Y+p.L210W+p.R211K+p.W212X+p.T215Y+p.K223Q	Missense mutation	11	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CF, T39TS, M41L, K43Q, E44D, D67N, V75A, K102Q, D113DN, V118I, K122E, D123E, I135IT, S162Y, D177E, M184V, G196E, Q197QP, T200ADE, E203K, H208Y, L210W, R211K, W212X, T215Y, K223Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CF+p.T58N+p.K65KR+p.S68G+p.T69TI+p.K70R+p.V75I+p.F77L+p.K101E+p.K102KR+p.Y115F+p.F116Y+p.K122E+p.I135IV+p.E138EV+p.Q151M+p.S162C+p.P170PL+p.F171Y+p.K173N+p.Q174K+p.D177E+p.Y188L+p.H208HY+p.R211K+p.K219E	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CF, T58N, K65KR, S68G, T69TI, K70R, V75I, F77L, K101E, K102KR, Y115F, F116Y, K122E, I135IV, E138EV, Q151M, S162C, P170PL, F171Y, K173N, Q174K, D177E, Y188L, H208HY, R211K, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.S68G+p.K70R+p.V108I+p.D123E+p.I135T+p.I142V+p.D177E+p.M184V+p.T200A+p.R211K+p.T215F+p.K219E+p.E297K	Missense mutation	23	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, S68G, K70R, V108I, D123E, I135T, I142V, D177E, M184V, T200A, R211K, T215F, K219E, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K103N+p.K122KE+p.Q207E+p.T215Y+p.H221HY+p.L228LR+p.K238T	Missense mutation	16	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K103N, K122KE, Q207E, T215Y, H221HY, L228LR, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K103N+p.K122E+p.V179VI+p.G196K+p.T200TA+p.K219Q+p.L228H+p.K238T+p.V245K	Missense mutation	14	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K103N, K122E, V179VI, G196K, T200TA, K219Q, L228H, K238T, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.P170PL+p.I178M+p.Y188C+p.T200A+p.E204D+p.T215F+p.K219E+p.I244IV+p.S251T+p.R284K+p.T286A	Missense mutation	78	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, P170PL, I178M, Y188C, T200A, E204D, T215F, K219E, I244IV, S251T, R284K, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.L74LV+p.H96HN+p.L100LI+p.K103KN+p.V118I+p.D123E+p.N175Y+p.I178M+p.K201R+p.E203K+p.H208Y+p.L210LW+p.R211K+p.T215NY+p.K219N+p.K223E+p.V245M	Missense mutation	13	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, L74LV, H96HN, L100LI, K103KN, V118I, D123E, N175Y, I178M, K201R, E203K, H208Y, L210LW, R211K, T215NY, K219N, K223E, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K70R+p.L74V+p.K103N+p.V108VI+p.D121H+p.K122E+p.I135L+p.K166R+p.D177E+p.Y181YC+p.G190A+p.T200A+p.R211K+p.T215FI+p.K219Q	Missense mutation	18	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K70R, L74V, K103N, V108VI, D121H, K122E, I135L, K166R, D177E, Y181YC, G190A, T200A, R211K, T215FI, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K70R+p.L74V+p.V118I+p.M184V+p.K219Q	Missense mutation	71	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K70R, L74V, V118I, M184V, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69NY+p.K70R+p.E79ED+p.K103N+p.K104KQ+p.D110DN+p.D113DN+p.K122KE+p.D123N+p.N136NY+p.N137NI+p.I178M+p.Y181C+p.T215F+p.K219E+p.V245I	Missense mutation	16	Low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69NY, K70R, E79ED, K103N, K104KQ, D110DN, D113DN, K122KE, D123N, N136NY, N137NI, I178M, Y181C, T215F, K219E, V245I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28A+p.K64H+p.D67N+p.T69N+p.K70R+p.D121Y+p.K122E+p.M184V+p.R211K+p.T215F+p.D218E+p.K219Q+p.V245K+p.A272P+p.Q278QH+p.L283LI+p.R284RK+p.E297EK	Missense mutation	41	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28A, K64H, D67N, T69N, K70R, D121Y, K122E, M184V, R211K, T215F, D218E, K219Q, V245K, A272P, Q278QH, L283LI, R284RK, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EG+p.M41L+p.K43Q+p.S68SG+p.V118I+p.D123E+p.M184V+p.T200A+p.E203D+p.L210W+p.R211K+p.T215Y+p.L228X+p.D250DE+p.T286TA+p.I293IV	Missense mutation	24	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EG, M41L, K43Q, S68SG, V118I, D123E, M184V, T200A, E203D, L210W, R211K, T215Y, L228X, D250DE, T286TA, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.K32E+p.T39A+p.M41L+p.K43E+p.E44A+p.V60VI+p.D67N+p.T69D+p.V90VI+p.K103KN+p.V118I+p.K122E+p.I135T+p.D177E+p.M184MIV+p.G196E+p.L210W+p.R211K+p.T215Y+p.K219KR	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, K32E, T39A, M41L, K43E, E44A, V60VI, D67N, T69D, V90VI, K103KN, V118I, K122E, I135T, D177E, M184MIV, G196E, L210W, R211K, T215Y, K219KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.K32E+p.V35T+p.T39K+p.M41L+p.K43E+p.K49R+p.V60I+p.D67N+p.V75M+p.K101H+p.V118I+p.K122E+p.T139TAS+p.S162X+p.I178F+p.M184V+p.G190A+p.G196E+p.T200K+p.L205F+p.Q207E+p.H208Y+p.L210W+p.T215Y+p.K219KE+p.L228H+p.Q242L+p.A272P+p.A288AS+p.I293V+p.E297A	Missense mutation	38	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, K32E, V35T, T39K, M41L, K43E, K49R, V60I, D67N, V75M, K101H, V118I, K122E, T139TAS, S162X, I178F, M184V, G190A, G196E, T200K, L205F, Q207E, H208Y, L210W, T215Y, K219KE, L228H, Q242L, A272P, A288AS, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.V35I+p.K64Y+p.D67N+p.K70R+p.V75T+p.L100I+p.K103N+p.V118I+p.K122E+p.D177N+p.M184V+p.T200A+p.E203K+p.Q207E+p.R211RS+p.F214L+p.D218E+p.K219Q+p.L228H+p.E297EK	Missense mutation	41	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, V35I, K64Y, D67N, K70R, V75T, L100I, K103N, V118I, K122E, D177N, M184V, T200A, E203K, Q207E, R211RS, F214L, D218E, K219Q, L228H, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.D67N+p.K70R+p.R83K+p.K122P+p.G155GR+p.D177E+p.I178L+p.M184MV+p.Q207E+p.H208HY+p.L210W+p.R211RK+p.T215Y+p.K219KE	Missense mutation	74	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, D67N, K70R, R83K, K122P, G155GR, D177E, I178L, M184MV, Q207E, H208HY, L210W, R211RK, T215Y, K219KE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.E28EA+p.M41L+p.D67G+p.S68G+p.L74V+p.V118I+p.K122E+p.I142IV+p.Q174N+p.V179I+p.M184V+p.G190Q+p.T200K+p.Q207E+p.L210W+p.R211I+p.T215Y+p.K219DN+p.Q222QR+p.L228H	Missense mutation	29	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, E28EA, M41L, D67G, S68G, L74V, V118I, K122E, I142IV, Q174N, V179I, M184V, G190Q, T200K, Q207E, L210W, R211I, T215Y, K219DN, Q222QR, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K11KR+p.V35M+p.T39A+p.E40S+p.M41L+p.K43E+p.V111I+p.D123E+p.I142V+p.D177E+p.I178L+p.Y181C+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219E+p.L228H+p.A272P+p.K277R+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K11KR, V35M, T39A, E40S, M41L, K43E, V111I, D123E, I142V, D177E, I178L, Y181C, H208Y, L210W, R211K, T215Y, K219E, L228H, A272P, K277R, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K20R+p.V35M+p.T39A+p.M41L+p.G45E+p.V60I+p.D67NS+p.T69D+p.K70R+p.Q91QR+p.L100I+p.K103N+p.V118I+p.D123E+p.I135T+p.D177N+p.V179I+p.M184V+p.G196E+p.T200A+p.Q207E+p.F214L+p.T215F+p.K219Q+p.L228H+p.T286A+p.V292VI+p.I293V+p.E297A	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K20R, V35M, T39A, M41L, G45E, V60I, D67NS, T69D, K70R, Q91QR, L100I, K103N, V118I, D123E, I135T, D177N, V179I, M184V, G196E, T200A, Q207E, F214L, T215F, K219Q, L228H, T286A, V292VI, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.E44ED+p.T69D+p.L100I+p.K103N+p.V118VI+p.K122E+p.K166KR+p.V189I+p.G196E+p.I202V+p.E203K+p.E204Q+p.Q207E+p.L210W+p.T215Y+p.K219R+p.L228R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, E44ED, T69D, L100I, K103N, V118VI, K122E, K166KR, V189I, G196E, I202V, E203K, E204Q, Q207E, L210W, T215Y, K219R, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.K43KN+p.N54NH+p.T69X+p.R83RK+p.K103N+p.S162SN+p.D177E+p.I178IL+p.V179I+p.Y181C+p.T200A+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K223T+p.L228R+p.I270IV+p.A272P+p.E297KQ	Missense mutation	90	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, K43KN, N54NH, T69X, R83RK, K103N, S162SN, D177E, I178IL, V179I, Y181C, T200A, E203K, Q207E, L210W, R211K, T215Y, K223T, L228R, I270IV, A272P, E297KQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41ML+p.R83K+p.K103N+p.D177E+p.V179VI+p.Y181C+p.E194ED+p.T200A+p.E203K+p.Q207E+p.L210LW+p.R211K+p.T215Y+p.K223KT+p.L228R+p.A272P+p.E297K	Missense mutation	88	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41ML, R83K, K103N, D177E, V179VI, Y181C, E194ED, T200A, E203K, Q207E, L210LW, R211K, T215Y, K223KT, L228R, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T27S+p.M41L+p.K43E+p.E44A+p.D67N+p.L74I+p.V90I+p.K103N+p.V108I+p.V118I+p.K122E+p.I142V+p.A158S+p.S162HY+p.Y181G+p.V189VI+p.I202V+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.H221Y+p.K223Q+p.L228R+p.V245VE+p.A272P	Missense mutation	29	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T27S, M41L, K43E, E44A, D67N, L74I, V90I, K103N, V108I, V118I, K122E, I142V, A158S, S162HY, Y181G, V189VI, I202V, H208Y, L210W, R211K, T215Y, K219N, H221Y, K223Q, L228R, V245VE, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T39E+p.M41L+p.E44D+p.D67N+p.T69D+p.K103N+p.V111I+p.V118I+p.K122E+p.M184V+p.T200A+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.E224D+p.L228Q	Missense mutation	81	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T39E, M41L, E44D, D67N, T69D, K103N, V111I, V118I, K122E, M184V, T200A, E203D, Q207E, H208Y, L210W, R211K, T215Y, K219R, E224D, L228Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T39TA+p.M41L+p.E44D+p.D67DN+p.V75M+p.V118I+p.K122E+p.D123DN+p.A158S+p.K173KQ+p.M184V+p.T200A+p.Q207E+p.L210W+p.T215Y+p.V245K+p.A272P+p.T286A+p.E297A	Missense mutation	71	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T39TA, M41L, E44D, D67DN, V75M, V118I, K122E, D123DN, A158S, K173KQ, M184V, T200A, Q207E, L210W, T215Y, V245K, A272P, T286A, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T7P+p.M41L+p.E44D+p.V60I+p.D67N+p.K70R+p.W88WS+p.A98S+p.K103N+p.V118VI+p.K122P+p.I135V+p.S162SN+p.D177E+p.Y181YC+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219H+p.L228LH+p.A272P+p.E297A	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T7P, M41L, E44D, V60I, D67N, K70R, W88WS, A98S, K103N, V118VI, K122P, I135V, S162SN, D177E, Y181YC, H208HY, L210W, R211K, T215Y, K219H, L228LH, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V35VI+p.M41L+p.K43E+p.E44A+p.V60VI+p.K64R+p.D67N+p.K70R+p.A98AG+p.V118I+p.K122KE+p.I142IV+p.M184V+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.K219DE+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V35VI, M41L, K43E, E44A, V60VI, K64R, D67N, K70R, A98AG, V118I, K122KE, I142IV, M184V, Q207E, L210W, R211RK, T215Y, K219DE, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V8I+p.K11T+p.M16V+p.K20R+p.V35T+p.T39K+p.M41L+p.K43E+p.V60I+p.D67N+p.T69N+p.K70R+p.V106I+p.K122E+p.D123S+p.I167V+p.K173I+p.Q174K+p.D177E+p.Y181C+p.M184V+p.Q207A+p.R211S+p.T215F+p.K219E+p.H221Y+p.P226H+p.M230L+p.P236R	Missense mutation	50	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V8I, K11T, M16V, K20R, V35T, T39K, M41L, K43E, V60I, D67N, T69N, K70R, V106I, K122E, D123S, I167V, K173I, Q174K, D177E, Y181C, M184V, Q207A, R211S, T215F, K219E, H221Y, P226H, M230L, P236R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6DN+p.K20R+p.T39E+p.M41L+p.E44D+p.V60I+p.D67N+p.V118VI+p.K122E+p.T131TI+p.Q207E+p.L210W+p.T215Y+p.D218E+p.K219R+p.E248D+p.A272AP+p.T286A+p.E297K+p.I326V	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6DN, K20R, T39E, M41L, E44D, V60I, D67N, V118VI, K122E, T131TI, Q207E, L210W, T215Y, D218E, K219R, E248D, A272AP, T286A, E297K, I326V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.K20R+p.V35I+p.M41L+p.K43A+p.E44D+p.V60I+p.D67N+p.L74I+p.A98G+p.V118I+p.G196E+p.E203K+p.Q207DE+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223T	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, K20R, V35I, M41L, K43A, E44D, V60I, D67N, L74I, A98G, V118I, G196E, E203K, Q207DE, H208Y, L210W, R211K, T215Y, K219R, K223T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.M41L+p.K43E+p.E44A+p.D67N+p.L74I+p.A98S+p.V118I+p.K122E+p.E169D+p.D177DG+p.V179FI+p.Y181YC+p.M184V+p.G190A+p.G196E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q+p.L228H+p.Q242H+p.A288S+p.I293IV+p.E297K	Missense mutation	42	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, M41L, K43E, E44A, D67N, L74I, A98S, V118I, K122E, E169D, D177DG, V179FI, Y181YC, M184V, G190A, G196E, H208Y, L210W, R211K, T215Y, D218E, K219Q, L228H, Q242H, A288S, I293IV, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.M41L+p.K43Q+p.E44D+p.V60I+p.D67N+p.T69D+p.V75M+p.F77L+p.V118I+p.K122E+p.D123E+p.I135IV+p.T139K+p.D177E+p.G196E+p.T200TA+p.E203K+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, M41L, K43Q, E44D, V60I, D67N, T69D, V75M, F77L, V118I, K122E, D123E, I135IV, T139K, D177E, G196E, T200TA, E203K, L210W, R211K, T215Y, K219R, K223Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.P19PS+p.M41L+p.E44D+p.D67DN+p.K102R+p.V118I+p.K122E+p.I135T+p.E169K+p.K173E+p.M184V+p.T200A+p.E203K+p.Q207E+p.H208HY+p.L210W+p.T215Y+p.K223Q	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, P19PS, M41L, E44D, D67DN, K102R, V118I, K122E, I135T, E169K, K173E, M184V, T200A, E203K, Q207E, H208HY, L210W, T215Y, K223Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.T39TA+p.V60I+p.D67N+p.T69D+p.K70R+p.K104N+p.K122E+p.I135IT+p.E138EQ+p.K166R+p.V179E+p.Y181C+p.Y188L+p.T200A+p.I202V+p.H208Y+p.R211K+p.T215F+p.K219W+p.L228LH	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, T39TA, V60I, D67N, T69D, K70R, K104N, K122E, I135IT, E138EQ, K166R, V179E, Y181C, Y188L, T200A, I202V, H208Y, R211K, T215F, K219W, L228LH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K20KR+p.M41L+p.K43S+p.V118I+p.K122E+p.I135V+p.V179VI+p.M184V+p.T200A+p.E203D+p.L210W+p.R211RK+p.T215Y+p.L228H+p.A272P+p.R284K+p.T286TA	Missense mutation	32	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K20KR, M41L, K43S, V118I, K122E, I135V, V179VI, M184V, T200A, E203D, L210W, R211RK, T215Y, L228H, A272P, R284K, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K20R+p.K32KR+p.V35I+p.M41L+p.K43E+p.E44A+p.D67N+p.T69D+p.A98G+p.K101E+p.V106I+p.V108VI+p.V111VA+p.V118I+p.K122E+p.S162Y+p.V179F+p.Y181C+p.M184V+p.G190A+p.H198HY+p.T200K+p.E204K+p.L210W+p.R211D+p.T215Y+p.K219N+p.K223Q+p.L228H	Missense mutation	53	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K20R, K32KR, V35I, M41L, K43E, E44A, D67N, T69D, A98G, K101E, V106I, V108VI, V111VA, V118I, K122E, S162Y, V179F, Y181C, M184V, G190A, H198HY, T200K, E204K, L210W, R211D, T215Y, K219N, K223Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.M41L+p.E44D+p.D67N+p.L74I+p.K82KE+p.L100I+p.K103N+p.V118I+p.K122E+p.Q174E+p.M184V+p.D192DN+p.T200A+p.L210W+p.R211K+p.T215Y+p.D218DE+p.K219N+p.D237DN+p.V245T+p.A272S+p.T286A+p.A288S+p.E297Q	Missense mutation	58	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, M41L, E44D, D67N, L74I, K82KE, L100I, K103N, V118I, K122E, Q174E, M184V, D192DN, T200A, L210W, R211K, T215Y, D218DE, K219N, D237DN, V245T, A272S, T286A, A288S, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.V35I+p.T39A+p.I47F+p.D67N+p.T69N+p.K70R+p.A98S+p.Y181C+p.T200A+p.R211K+p.F214L+p.T215F+p.D218E+p.K219E+p.E224D+p.L228H+p.V245M+p.T286P	Missense mutation	14	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, V35I, T39A, I47F, D67N, T69N, K70R, A98S, Y181C, T200A, R211K, F214L, T215F, D218E, K219E, E224D, L228H, V245M, T286P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.V8VI+p.K20R+p.K64H+p.D67N+p.T69N+p.K70R+p.V118VI+p.K122E+p.S162D+p.D177E+p.T200A+p.E203K+p.Q207E+p.F214L+p.K219E+p.V245VM	Missense mutation	39	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, V8VI, K20R, K64H, D67N, T69N, K70R, V118VI, K122E, S162D, D177E, T200A, E203K, Q207E, F214L, K219E, V245VM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6X+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.L74V+p.K122E+p.I178L+p.M184V+p.T200X+p.Q207E+p.T215F+p.K219Q+p.L228H+p.M230L+p.A272T+p.A288S+p.I293IV	Missense mutation	25	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6X, M41L, V60I, D67N, T69D, K70R, L74V, K122E, I178L, M184V, T200X, Q207E, T215F, K219Q, L228H, M230L, A272T, A288S, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I2IV+p.M16MV+p.K20R+p.V35M+p.T39A+p.M41L+p.K43A+p.E44D+p.D67N+p.Q91QR+p.V118I+p.K122KE+p.I135T+p.S162Y+p.K173KQ+p.D177E+p.M184V+p.L193LF+p.E194EK+p.G196E+p.I202V+p.E203A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q+p.L228H+p.A288S+p.E297Q	Missense mutation	45	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I2IV, M16MV, K20R, V35M, T39A, M41L, K43A, E44D, D67N, Q91QR, V118I, K122KE, I135T, S162Y, K173KQ, D177E, M184V, L193LF, E194EK, G196E, I202V, E203A, H208Y, L210W, R211K, T215Y, D218E, K219Q, L228H, A288S, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I2V+p.K20R+p.V35I+p.T39A+p.M41L+p.E44D+p.K49R+p.V60I+p.D67N+p.T69D+p.K70R+p.V75M+p.K103N+p.V118I+p.K122E+p.I135T+p.S162D+p.V179I+p.Y181C+p.G196E+p.L210W+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228H+p.T286A+p.E297K	Missense mutation	58	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I2V, K20R, V35I, T39A, M41L, E44D, K49R, V60I, D67N, T69D, K70R, V75M, K103N, V118I, K122E, I135T, S162D, V179I, Y181C, G196E, L210W, F214L, T215F, D218E, K219Q, L228H, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31L+p.V35E+p.T39S+p.M41L+p.K43E+p.E44A+p.D67N+p.V118I+p.K122P+p.E138G+p.D177E+p.V179E+p.T200A+p.E203D+p.H208Y+p.L210W+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31L, V35E, T39S, M41L, K43E, E44A, D67N, V118I, K122P, E138G, D177E, V179E, T200A, E203D, H208Y, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31L+p.V35I+p.T39A+p.M41L+p.K43Q+p.E44D+p.K49R+p.D67N+p.V75I+p.A98G+p.K101P+p.V111I+p.V118I+p.T131TA+p.Q174QR+p.I178L+p.M184I+p.I195L+p.G196E+p.I202V+p.E203K+p.Q207QH+p.H208F+p.L210W+p.R211E+p.T215Y+p.K219R+p.K223Q+p.F227L+p.L228Q	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31L, V35I, T39A, M41L, K43Q, E44D, K49R, D67N, V75I, A98G, K101P, V111I, V118I, T131TA, Q174QR, I178L, M184I, I195L, G196E, I202V, E203K, Q207QH, H208F, L210W, R211E, T215Y, K219R, K223Q, F227L, L228Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I37IV+p.M41L+p.K64KM+p.D67H+p.S68G+p.T69N+p.K70G+p.D121Y+p.K122E+p.I135T+p.I142V+p.S162CY+p.Q174QK+p.D177N+p.I178M+p.M184V+p.T200A+p.Q207E+p.R211RK+p.T215F+p.K219KT+p.H221HY+p.R284RK+p.E297K	Missense mutation	32	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I37IV, M41L, K64KM, D67H, S68G, T69N, K70G, D121Y, K122E, I135T, I142V, S162CY, Q174QK, D177N, I178M, M184V, T200A, Q207E, R211RK, T215F, K219KT, H221HY, R284RK, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5IM+p.E6EK+p.V35VI+p.D67N+p.S68G+p.K70R+p.A98S+p.K104KN+p.D123E+p.A158AS+p.Y188L+p.T200I+p.T215Y+p.K219E+p.E224ED	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5IM, E6EK, V35VI, D67N, S68G, K70R, A98S, K104KN, D123E, A158AS, Y188L, T200I, T215Y, K219E, E224ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5IV+p.T27TA+p.M41L+p.K43N+p.E44D+p.L74I+p.A98S+p.K101KQ+p.K103N+p.V108VI+p.V111I+p.F116FL+p.V118I+p.K122P+p.D123E+p.I135IM+p.D177E+p.V179I+p.M184V+p.G196E+p.E203ED+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.L228R	Missense mutation	65	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5IV, T27TA, M41L, K43N, E44D, L74I, A98S, K101KQ, K103N, V108VI, V111I, F116FL, V118I, K122P, D123E, I135IM, D177E, V179I, M184V, G196E, E203ED, Q207E, H208Y, L210W, R211K, T215Y, K219R, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.K20R+p.M41L+p.K43KR+p.E44ED+p.S68G+p.G93GR+p.A98G+p.K102KR+p.D123E+p.D177E+p.V179I+p.M184V+p.Y188L+p.T200IL+p.L210LS+p.R211K+p.F214FL+p.T215Y+p.L228LH+p.Q242QR+p.E248ED	Missense mutation	24	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, K20R, M41L, K43KR, E44ED, S68G, G93GR, A98G, K102KR, D123E, D177E, V179I, M184V, Y188L, T200IL, L210LS, R211K, F214FL, T215Y, L228LH, Q242QR, E248ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.M41L+p.L74LV+p.K103KN+p.V108VI+p.V118VI+p.K122E+p.I178L+p.M184V+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.M230L	Missense mutation	93	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, M41L, L74LV, K103KN, V108VI, V118VI, K122E, I178L, M184V, H208Y, L210W, R211K, T215Y, K219N, M230L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.T39A+p.K64H+p.D67N+p.T69N+p.K70R+p.V118VI+p.D123E+p.M184V+p.T200TA+p.F214L+p.D218E+p.K219Q+p.L228X	Missense mutation	19	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, T39A, K64H, D67N, T69N, K70R, V118VI, D123E, M184V, T200TA, F214L, D218E, K219Q, L228X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.T39TA+p.M41L+p.K43KQ+p.A62AV+p.S68SG+p.V75VI+p.F116FY+p.V118I+p.I135T+p.Q151QL+p.V179I+p.M184MV+p.Y188L+p.H208HY+p.R211RK+p.F214L+p.T215F+p.K223KQ+p.L228H	Missense mutation	30	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, T39TA, M41L, K43KQ, A62AV, S68SG, V75VI, F116FY, V118I, I135T, Q151QL, V179I, M184MV, Y188L, H208HY, R211RK, F214L, T215F, K223KQ, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KT+p.T39TA+p.M41L+p.K43E+p.E44ED+p.V75VM+p.K103N+p.V106VI+p.V118I+p.K122E+p.R172RG+p.D177X+p.G196E+p.T200TI+p.E203KQ+p.Q207D+p.H208HY+p.L210W+p.R211RK+p.T215Y+p.H221HY+p.L228H+p.T286P+p.A288S+p.E297KR	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KT, T39TA, M41L, K43E, E44ED, V75VM, K103N, V106VI, V118I, K122E, R172RG, D177X, G196E, T200TI, E203KQ, Q207D, H208HY, L210W, R211RK, T215Y, H221HY, L228H, T286P, A288S, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11Q+p.K20R+p.V35T+p.T39TA+p.M41L+p.K43KQ+p.K101P+p.K103S+p.K122E+p.D123N+p.T200X+p.E203D+p.H208Y+p.R211K+p.T215Y+p.K219R+p.L228HR+p.V292I+p.E297A	Missense mutation	25	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11Q, K20R, V35T, T39TA, M41L, K43KQ, K101P, K103S, K122E, D123N, T200X, E203D, H208Y, R211K, T215Y, K219R, L228HR, V292I, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.E28K+p.K32E+p.T39K+p.M41L+p.K43E+p.E44D+p.V60I+p.D67N+p.T69D+p.R83K+p.K102M+p.V106I+p.V118I+p.K122P+p.I135T+p.Q174R+p.M184V+p.Y188L+p.Q207E+p.L210W+p.T215Y+p.D237E+p.A272P+p.T286A+p.E297T	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, E28K, K32E, T39K, M41L, K43E, E44D, V60I, D67N, T69D, R83K, K102M, V106I, V118I, K122P, I135T, Q174R, M184V, Y188L, Q207E, L210W, T215Y, D237E, A272P, T286A, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.K20R+p.K64H+p.D67N+p.T69N+p.K70R+p.L74I+p.K103N+p.D121Y+p.K122E+p.I135M+p.K166R+p.D177E+p.M184V+p.G196E+p.T200L+p.R211K+p.T215F+p.K219Q+p.L228H+p.A272AP+p.K277R+p.T286A+p.A288P+p.E297R	Missense mutation	39	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, K20R, K64H, D67N, T69N, K70R, L74I, K103N, D121Y, K122E, I135M, K166R, D177E, M184V, G196E, T200L, R211K, T215F, K219Q, L228H, A272AP, K277R, T286A, A288P, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.V21I+p.V35T+p.M41L+p.K43E+p.E44A+p.T69D+p.R83K+p.K101Q+p.K103R+p.V108I+p.L109I+p.V118I+p.K122E+p.R172K+p.D177E+p.V179F+p.Y181C+p.G190A+p.G196E+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R+p.L228R+p.T286P+p.A288S+p.I293V+p.E297R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, V21I, V35T, M41L, K43E, E44A, T69D, R83K, K101Q, K103R, V108I, L109I, V118I, K122E, R172K, D177E, V179F, Y181C, G190A, G196E, L210W, R211K, T215Y, D218E, K219R, L228R, T286P, A288S, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.V35I+p.K46Q+p.K49R+p.A62V+p.V75I+p.F77L+p.V108I+p.Y115F+p.F116Y+p.D123E+p.Q151M+p.D177E+p.M184V+p.Q207N+p.R211K+p.A272AP+p.K277KR+p.A288S+p.I293V+p.K311R+p.Q334L+p.R358K+p.K390R+p.V435I+p.T470N+p.A554T+p.K558R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, V35I, K46Q, K49R, A62V, V75I, F77L, V108I, Y115F, F116Y, D123E, Q151M, D177E, M184V, Q207N, R211K, A272AP, K277KR, A288S, I293V, K311R, Q334L, R358K, K390R, V435I, T470N, A554T, K558R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.D67DEG+p.S68G+p.T69TS+p.K70R+p.R211S+p.T215F+p.D218DE+p.K219Q+p.T240TI+p.V276VA+p.K281R+p.E297K	Missense mutation	20	Low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, D67DEG, S68G, T69TS, K70R, R211S, T215F, D218DE, K219Q, T240TI, V276VA, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.M41L+p.K43KQ+p.E44D+p.D67N+p.L74IV+p.K101EQ+p.V118I+p.K122E+p.I135IT+p.I142IV+p.V179A+p.Y181C+p.M184V+p.V189I+p.G190A+p.G196E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228LH	Missense mutation	27	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, M41L, K43KQ, E44D, D67N, L74IV, K101EQ, V118I, K122E, I135IT, I142IV, V179A, Y181C, M184V, V189I, G190A, G196E, H208Y, L210W, R211K, T215Y, K219N, L228LH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.M41L+p.P52PS+p.V60I+p.D67N+p.T69D+p.K70R+p.A98S+p.K101Q+p.K103N+p.V118I+p.K122E+p.D123N+p.I135T+p.K166R+p.M184V+p.T200TA+p.I202V+p.F214L+p.T215F+p.K219Q+p.L228H	Missense mutation	52	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, M41L, P52PS, V60I, D67N, T69D, K70R, A98S, K101Q, K103N, V118I, K122E, D123N, I135T, K166R, M184V, T200TA, I202V, F214L, T215F, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.M41L+p.V60VI+p.R83RK+p.V90I+p.S162Y+p.V179I+p.M184V+p.T200TI+p.E203ED+p.R211T+p.T215Y+p.R284K+p.T286A+p.E297K	Missense mutation	16	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, M41L, V60VI, R83RK, V90I, S162Y, V179I, M184V, T200TI, E203ED, R211T, T215Y, R284K, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.T39TA+p.M41L+p.E44D+p.D67N+p.K103N+p.V118I+p.K122E+p.I142V+p.Q174QR+p.D177E+p.M184V+p.G196E+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219N+p.L234LP+p.V245K+p.A272P+p.R284RK	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, T39TA, M41L, E44D, D67N, K103N, V118I, K122E, I142V, Q174QR, D177E, M184V, G196E, Q207E, L210W, R211K, T215Y, K219N, L234LP, V245K, A272P, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.T39X+p.M41ML+p.K43KEQ+p.K49R+p.I50V+p.V60I+p.D67N+p.T69N+p.K70R+p.L80LF+p.D123E+p.E138EG+p.A158AS+p.S162X+p.K166KQ+p.E169D+p.D177E+p.G196D+p.T215F+p.K219Q	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, T39X, M41ML, K43KEQ, K49R, I50V, V60I, D67N, T69N, K70R, L80LF, D123E, E138EG, A158AS, S162X, K166KQ, E169D, D177E, G196D, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V21VI+p.V35M+p.T39A+p.M41L+p.K43Q+p.E44D+p.D67G+p.T69D+p.V118I+p.K122E+p.I135IM+p.M184V+p.G196E+p.T200A+p.E203D+p.L205F+p.Q207E+p.H208Y+p.L210LW+p.R211K+p.T215Y+p.D218E+p.K219R	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V21VI, V35M, T39A, M41L, K43Q, E44D, D67G, T69D, V118I, K122E, I135IM, M184V, G196E, T200A, E203D, L205F, Q207E, H208Y, L210LW, R211K, T215Y, D218E, K219R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35L+p.M41L+p.V60VI+p.D67N+p.T69N+p.K70R+p.A98G+p.K101Q+p.K102R+p.K103R+p.K122E+p.D123N+p.E138K+p.Q174A+p.V179D+p.Y181C+p.G190A+p.L210W+p.T215F+p.K219E+p.L228N	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35L, M41L, V60VI, D67N, T69N, K70R, A98G, K101Q, K102R, K103R, K122E, D123N, E138K, Q174A, V179D, Y181C, G190A, L210W, T215F, K219E, L228N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.D67DEG+p.S68SG+p.T69TS+p.K70R+p.R211S+p.T215FI+p.D218DE+p.K219Q+p.K281R+p.E297K	Missense mutation	25	Low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, D67DEG, S68SG, T69TS, K70R, R211S, T215FI, D218DE, K219Q, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E28K+p.K32E+p.T39A+p.M41L+p.E44D+p.D67N+p.V118I+p.D123E+p.I135K+p.Q174R+p.D177E+p.M184V+p.G196E+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E+p.E297K	Missense mutation	35	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E28K, K32E, T39A, M41L, E44D, D67N, V118I, D123E, I135K, Q174R, D177E, M184V, G196E, T200A, L210W, R211K, T215Y, V245E, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E44D+p.D67~+p.S68G+p.T69G+p.K70R+p.R83K+p.K101E+p.K103R+p.K166R+p.G190S+p.T215I+p.K219Q+p.V245K+p.A272P	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E44D, D67~, S68G, T69G, K70R, R83K, K101E, K103R, K166R, G190S, T215I, K219Q, V245K, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.D67N+p.K70R+p.D123E+p.I135T+p.D177E+p.M184V+p.Y188L+p.G196E+p.I202V+p.H208HY+p.T215F+p.K219Q	Missense mutation	55	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, D67N, K70R, D123E, I135T, D177E, M184V, Y188L, G196E, I202V, H208HY, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.E44D+p.A62V+p.D67N+p.T69N+p.V75I+p.V106A+p.L109I+p.I142V+p.D177E+p.H208Y+p.L210W+p.R211K+p.D218E+p.K219N+p.F227L	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, E44D, A62V, D67N, T69N, V75I, V106A, L109I, I142V, D177E, H208Y, L210W, R211K, D218E, K219N, F227L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.T69D+p.K103N+p.V108VI+p.V118I+p.I135T+p.E169D+p.M184V+p.G196E+p.L210W+p.R211K+p.T215Y+p.K219KE+p.E248D+p.A288S+p.E297K	Missense mutation	26	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43E, E44A, V60I, D67N, T69D, K103N, V108VI, V118I, I135T, E169D, M184V, G196E, L210W, R211K, T215Y, K219KE, E248D, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43E+p.E44D+p.D67N+p.L74V+p.A98G+p.V118I+p.K122E+p.I135T+p.R172RK+p.I178M+p.M184MV+p.V189I+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.A272P	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43E, E44D, D67N, L74V, A98G, V118I, K122E, I135T, R172RK, I178M, M184MV, V189I, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219R, K223Q, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43N+p.D67G+p.T69N+p.K70R+p.L74V+p.K103N+p.I142V+p.Y181C+p.G196E+p.I202V+p.T215F+p.K219Q+p.H221HY+p.V245EK+p.A288S	Missense mutation	11	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43N, D67G, T69N, K70R, L74V, K103N, I142V, Y181C, G196E, I202V, T215F, K219Q, H221HY, V245EK, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43N+p.E44D+p.K64H+p.D67G+p.K101E+p.V118I+p.D123E+p.E138A+p.I178M+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43N, E44D, K64H, D67G, K101E, V118I, D123E, E138A, I178M, L210W, R211K, T215Y, D218E, K219R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.V60VI+p.K103N+p.V118VI+p.K122E+p.D123DG+p.V179VI+p.T200A+p.L210W+p.R211K+p.T215Y+p.I270IF	Missense mutation	82	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, V60VI, K103N, V118VI, K122E, D123DG, V179VI, T200A, L210W, R211K, T215Y, I270IF.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.D67N+p.T69X+p.K70R+p.K122E+p.E169D+p.M184V+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215F+p.K219Q+p.K238KR	Missense mutation	21	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, D67N, T69X, K70R, K122E, E169D, M184V, G196E, E203K, Q207E, H208Y, L210W, R211K, T215F, K219Q, K238KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.K49R+p.V60I+p.D67N+p.T69D+p.K70R+p.V75M+p.K103R+p.D123E+p.S162D+p.K166R+p.I178M+p.H208Y+p.L210F+p.R211K+p.T215F+p.K219Q+p.L228H+p.K277R+p.I293V+p.T296S+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, K49R, V60I, D67N, T69D, K70R, V75M, K103R, D123E, S162D, K166R, I178M, H208Y, L210F, R211K, T215F, K219Q, L228H, K277R, I293V, T296S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39E+p.M41L+p.E44D+p.D67N+p.T69D+p.L74I+p.V75X+p.A98G+p.K101Q+p.V108I+p.V118I+p.K122E+p.I135T+p.T165I+p.V179VA+p.Y181C+p.G190A+p.G196E+p.E203KT+p.H208F+p.L210RW+p.R211K+p.T215Y+p.H221Y+p.L228LF	Missense mutation	29	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39E, M41L, E44D, D67N, T69D, L74I, V75X, A98G, K101Q, V108I, V118I, K122E, I135T, T165I, V179VA, Y181C, G190A, G196E, E203KT, H208F, L210RW, R211K, T215Y, H221Y, L228LF.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39K+p.M41L+p.K43E+p.S68G+p.T69N+p.L74LV+p.K103S+p.V111I+p.V118VI+p.K122E+p.D123DE+p.Q174R+p.I178L+p.M184V+p.T200E+p.L210W+p.T215Y	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39K, M41L, K43E, S68G, T69N, L74LV, K103S, V111I, V118VI, K122E, D123DE, Q174R, I178L, M184V, T200E, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39N+p.M41L+p.K43E+p.E44A+p.D67N+p.L74V+p.L100I+p.K103N+p.K122E+p.I142V+p.D177X+p.M184V+p.G196E+p.I202IV+p.H208Y+p.L210W+p.R211RK+p.T215Y+p.K219N+p.L228H	Missense mutation	35	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39N, M41L, K43E, E44A, D67N, L74V, L100I, K103N, K122E, I142V, D177X, M184V, G196E, I202IV, H208Y, L210W, R211RK, T215Y, K219N, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.T39E+p.M41L+p.E44D+p.D67N+p.T69D+p.V118I+p.K122KE+p.D123DN+p.S162D+p.D177E+p.I178L+p.M184V+p.G196Q+p.T200A+p.E203D+p.Q207E+p.H208F+p.L210W+p.R211K+p.T215Y+p.K219R+p.H221HY	Missense mutation	63	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, T39E, M41L, E44D, D67N, T69D, V118I, K122KE, D123DN, S162D, D177E, I178L, M184V, G196Q, T200A, E203D, Q207E, H208F, L210W, R211K, T215Y, K219R, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35IKM+p.T39A+p.E40X+p.M41L+p.K43KE+p.V60I+p.D67N+p.T69TS+p.L74LI+p.A98AG+p.K102QR+p.V118VI+p.I135V+p.S162D+p.E169D+p.D177DG+p.V179VI+p.M184V+p.G196E+p.T200TA+p.E203EG+p.H208HY+p.L210LW+p.R211RK+p.T215FY+p.K219Q+p.K223KQ+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35IKM, T39A, E40X, M41L, K43KE, V60I, D67N, T69TS, L74LI, A98AG, K102QR, V118VI, I135V, S162D, E169D, D177DG, V179VI, M184V, G196E, T200TA, E203EG, H208HY, L210LW, R211RK, T215FY, K219Q, K223KQ, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35L+p.V60I+p.K64N+p.D67N+p.K70R+p.L74V+p.L100I+p.K103N+p.I135V+p.K166KR+p.Q174H+p.P176PS+p.E203EK+p.Q207E+p.R211K+p.K219Q+p.L228LH+p.A272P+p.T286A	Missense mutation	11	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35L, V60I, K64N, D67N, K70R, L74V, L100I, K103N, I135V, K166KR, Q174H, P176PS, E203EK, Q207E, R211K, K219Q, L228LH, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.E40D+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.A98S+p.K104N+p.I135T+p.E138K+p.S162Y+p.R172RK+p.M184V+p.R211Q+p.T215CF+p.K219Q+p.L228H+p.V245E+p.T286A+p.V292VI+p.E297K	Missense mutation	54	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39A, E40D, M41L, V60I, D67N, T69D, K70R, A98S, K104N, I135T, E138K, S162Y, R172RK, M184V, R211Q, T215CF, K219Q, L228H, V245E, T286A, V292VI, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.E40ED+p.M41L+p.V60VI+p.D67N+p.T69DN+p.K70R+p.A98S+p.K104N+p.I135T+p.S162Y+p.G196GE+p.E203EK+p.R211Q+p.T215F+p.K219Q+p.L228H+p.V245E+p.T286A+p.V292I+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39A, E40ED, M41L, V60VI, D67N, T69DN, K70R, A98S, K104N, I135T, S162Y, G196GE, E203EK, R211Q, T215F, K219Q, L228H, V245E, T286A, V292I, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.M41L+p.K43E+p.E44D+p.K64KR+p.D67N+p.L74V+p.A98G+p.K102Q+p.K103N+p.V111VIM+p.V118I+p.D123E+p.Q174R+p.D177E+p.Y181C+p.M184V+p.V189VI+p.G190A+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K223Q+p.A272P+p.T286A+p.E297R	Missense mutation	23	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39A, M41L, K43E, E44D, K64KR, D67N, L74V, A98G, K102Q, K103N, V111VIM, V118I, D123E, Q174R, D177E, Y181C, M184V, V189VI, G190A, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, K223Q, A272P, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39TA+p.M41L+p.S68G+p.K70KN+p.L74I+p.A98G+p.V118VI+p.K122E+p.I142IV+p.Q174QR+p.D177E+p.I178M+p.T200TA+p.E203EK+p.Q207E+p.H208HY+p.L210LW+p.R211RK+p.T215Y+p.V245MT+p.T286TA	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39TA, M41L, S68G, K70KN, L74I, A98G, V118VI, K122E, I142IV, Q174QR, D177E, I178M, T200TA, E203EK, Q207E, H208HY, L210LW, R211RK, T215Y, V245MT, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35MR+p.T39A+p.E40ED+p.K43Q+p.K64H+p.D67G+p.T69N+p.K70R+p.L74I+p.A98AG+p.K103N+p.V108I+p.V118I+p.A158S+p.S162SC+p.T165TI+p.E169D+p.M184V+p.R211RK+p.F214L+p.T215X+p.P217PS+p.K219Q+p.L228H+p.K238T	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35MR, T39A, E40ED, K43Q, K64H, D67G, T69N, K70R, L74I, A98AG, K103N, V108I, V118I, A158S, S162SC, T165TI, E169D, M184V, R211RK, F214L, T215X, P217PS, K219Q, L228H, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VL+p.M41L+p.V60I+p.K64Y+p.K66R+p.D67N+p.K70R+p.L74I+p.A98G+p.K103N+p.V118I+p.K122E+p.D123S+p.S162SN+p.Y181C+p.G190A+p.G196E+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228H+p.D237DG	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VL, M41L, V60I, K64Y, K66R, D67N, K70R, L74I, A98G, K103N, V118I, K122E, D123S, S162SN, Y181C, G190A, G196E, F214L, T215F, D218E, K219Q, L228H, D237DG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VM+p.I37L+p.M41L+p.K43KN+p.D67N+p.T69N+p.K70R+p.L74LI+p.V90I+p.K122KE+p.N175ND+p.D177DN+p.I178M+p.I180IV+p.Y181YC+p.G190A+p.Q207E+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.L228H+p.K238KT+p.V245K+p.A272P+p.R284RK+p.A288S	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VM, I37L, M41L, K43KN, D67N, T69N, K70R, L74LI, V90I, K122KE, N175ND, D177DN, I178M, I180IV, Y181YC, G190A, Q207E, H208Y, R211K, T215F, D218E, K219Q, L228H, K238KT, V245K, A272P, R284RK, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.V35KR+p.T39D+p.M41L+p.E44ED+p.V60I+p.D67N+p.T69D+p.K70KR+p.K103KN+p.V118VI+p.K122E+p.I135T+p.M184V+p.H208HY+p.R211K+p.T215Y+p.D218E+p.K219Q+p.K223KE+p.L228H+p.Q242QH+p.T286A+p.A288AT+p.I293IV	Missense mutation	58	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, V35KR, T39D, M41L, E44ED, V60I, D67N, T69D, K70KR, K103KN, V118VI, K122E, I135T, M184V, H208HY, R211K, T215Y, D218E, K219Q, K223KE, L228H, Q242QH, T286A, A288AT, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.E53D+p.K64R+p.D67E+p.T69S_SS+p.V118I+p.K122E+p.I135T+p.T139I+p.I142IT+p.Y188L+p.V189VI+p.T200TA+p.T215Y+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, E53D, K64R, D67E, T69S_SS, V118I, K122E, I135T, T139I, I142IT, Y188L, V189VI, T200TA, T215Y, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64H+p.K66R+p.D67G+p.T69N+p.K70R+p.K103N+p.V118I+p.K122E+p.D123DN+p.I135T+p.Y188H+p.G196GE+p.T200I+p.F214L+p.T215I+p.K219Q+p.K223KQ+p.L228R+p.Q278QH+p.P294T+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64H, K66R, D67G, T69N, K70R, K103N, V118I, K122E, D123DN, I135T, Y188H, G196GE, T200I, F214L, T215I, K219Q, K223KQ, L228R, Q278QH, P294T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L34I+p.T39A+p.M41L+p.K82R+p.K103N+p.I132V+p.V179I+p.Y181C+p.M184V+p.G196E+p.F214L+p.T215Y+p.P225H+p.L228R	Missense mutation	25	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L34I, T39A, M41L, K82R, K103N, I132V, V179I, Y181C, M184V, G196E, F214L, T215Y, P225H, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L34LI+p.T39A+p.M41L+p.K122E+p.D123DG+p.I135IT+p.I142V+p.V179I+p.M184V+p.Y188L+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219KE+p.Q269QH	Missense mutation	34	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L34LI, T39A, M41L, K122E, D123DG, I135IT, I142V, V179I, M184V, Y188L, H208Y, L210W, R211K, T215Y, K219KE, Q269QH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16V+p.K20R+p.V35VL+p.T39TA+p.M41L+p.E53D+p.V60VI+p.D67N+p.T69TN+p.K70R+p.L74LV+p.V90I+p.L100I+p.K103N+p.I135V+p.D177DN+p.G196E+p.T200TI+p.T215F+p.K219Q+p.L228H+p.Q242QH+p.A272P+p.K277KR+p.E297P	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16V, K20R, V35VL, T39TA, M41L, E53D, V60VI, D67N, T69TN, K70R, L74LV, V90I, L100I, K103N, I135V, D177DN, G196E, T200TI, T215F, K219Q, L228H, Q242QH, A272P, K277KR, E297P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62AV+p.K103N+p.K122E+p.Q207E+p.R211K+p.T215Y+p.A272P+p.L283I+p.R284K+p.E297EQ	Missense mutation	46	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62AV, K103N, K122E, Q207E, R211K, T215Y, A272P, L283I, R284K, E297EQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.D67N+p.T69D+p.V75I+p.R83K+p.K103N+p.V108I+p.F116Y+p.D123E+p.Q151M+p.K166R+p.D177E+p.I202V+p.L210W+p.T215Y+p.M230L+p.A272P+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, D67N, T69D, V75I, R83K, K103N, V108I, F116Y, D123E, Q151M, K166R, D177E, I202V, L210W, T215Y, M230L, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.V75I+p.K101E+p.K102R+p.K122E+p.I135T+p.N136NH+p.E138A+p.I142T+p.S162C+p.G190S+p.G196E+p.T215Y+p.D237N	Missense mutation	11	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, V75I, K101E, K102R, K122E, I135T, N136NH, E138A, I142T, S162C, G190S, G196E, T215Y, D237N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D121Y+p.K122E+p.I178IL+p.M184V+p.G196E+p.Q207E+p.L210W+p.T215Y+p.V245E+p.E248D+p.A272P+p.K277KR+p.E297EK	Missense mutation	20	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D121Y, K122E, I178IL, M184V, G196E, Q207E, L210W, T215Y, V245E, E248D, A272P, K277KR, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67DN+p.K102KR+p.K103KN+p.V118VI+p.D121H+p.K122E+p.I142IV+p.K166KQ+p.E169D+p.I178L+p.Y181YC+p.I202IM+p.H208HY+p.L210W+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67DN, K102KR, K103KN, V118VI, D121H, K122E, I142IV, K166KQ, E169D, I178L, Y181YC, I202IM, H208HY, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.K103S+p.M184V+p.G190A+p.H208Y+p.L210W+p.T215Y+p.K219E	Missense mutation	23	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, K103S, M184V, G190A, H208Y, L210W, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.M184V+p.H208Y+p.T215F+p.K219E	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, M184V, H208Y, T215F, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.M184V+p.H208Y+p.L210W+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, M184V, H208Y, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.A98G+p.K101E+p.V106I+p.V108I+p.V118I+p.Y181C+p.M184V+p.G190A+p.Q197E+p.T200A+p.E203D+p.H208F+p.L210W+p.R211K+p.T215Y+p.K219N+p.H221Y	Missense mutation	55	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, A98G, K101E, V106I, V108I, V118I, Y181C, M184V, G190A, Q197E, T200A, E203D, H208F, L210W, R211K, T215Y, K219N, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.K70R+p.L74V+p.K103KN+p.V108VI+p.V111VL+p.D113DN+p.V118I+p.K122E+p.I135T+p.G152X+p.I178M+p.Y181C+p.G190A+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219E+p.L228H	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, K70R, L74V, K103KN, V108VI, V111VL, D113DN, V118I, K122E, I135T, G152X, I178M, Y181C, G190A, H208HY, L210W, R211K, T215Y, K219E, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.K70R+p.V75M+p.V118VI+p.Y181C+p.L210W+p.T215Y+p.K219Q	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, K70R, V75M, V118VI, Y181C, L210W, T215Y, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.T69D+p.I94IL+p.V118I+p.K122E+p.I142IT+p.M184V+p.T200A+p.E203K+p.Q207A+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223E+p.L228CR+p.A288AS	Missense mutation	33	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, T69D, I94IL, V118I, K122E, I142IT, M184V, T200A, E203K, Q207A, H208HY, L210W, R211K, T215Y, K219N, K223E, L228CR, A288AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.T69D+p.L74LI+p.V75A+p.K103N+p.V111VL+p.V118I+p.D123E+p.Y181C+p.M184V+p.H208F+p.L210W+p.R211K+p.T215Y+p.H221Y	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, T69D, L74LI, V75A, K103N, V111VL, V118I, D123E, Y181C, M184V, H208F, L210W, R211K, T215Y, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.S68G+p.A98AG+p.V118VI+p.I135T+p.M184V+p.L210W+p.T215Y+p.D218DE	Missense mutation	68	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, S68G, A98AG, V118VI, I135T, M184V, L210W, T215Y, D218DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.T69D+p.A98G+p.K101E+p.V106VI+p.V118I+p.D123E+p.S162D+p.D177E+p.V179VI+p.Y181C+p.M184V+p.G190A+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.L228R	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, T69D, A98G, K101E, V106VI, V118I, D123E, S162D, D177E, V179VI, Y181C, M184V, G190A, E203K, H208Y, L210W, R211K, T215Y, K219R, K223E, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.T69D+p.L74V+p.K103N+p.V108I+p.V118I+p.Y181C+p.M184V+p.H208Y+p.L210W+p.T215Y+p.K219R+p.F227L	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, T69D, L74V, K103N, V108I, V118I, Y181C, M184V, H208Y, L210W, T215Y, K219R, F227L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.V60I+p.D67G+p.T69D+p.V75L+p.V106A+p.V108I+p.V118I+p.K122E+p.D123E+p.I135T+p.I142V+p.Q174QR+p.M184V+p.I195IV+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.F227L+p.V245K+p.A272P+p.E297EK	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, V60I, D67G, T69D, V75L, V106A, V108I, V118I, K122E, D123E, I135T, I142V, Q174QR, M184V, I195IV, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219R, K223E, F227L, V245K, A272P, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.V60I+p.K66R+p.D67N+p.T69D+p.L74V+p.K101E+p.V118I+p.K122E+p.I135T+p.Y181C+p.G196E+p.T200I+p.E203K+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223E+p.L228R+p.M230L+p.A272P+p.T286TP	Missense mutation	39	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, V60I, K66R, D67N, T69D, L74V, K101E, V118I, K122E, I135T, Y181C, G196E, T200I, E203K, Q207D, H208Y, L210W, R211K, T215Y, K219N, K223E, L228R, M230L, A272P, T286TP.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.V60I+p.L74LI+p.K102E+p.K103R+p.V118VI+p.I135T+p.S162CY+p.M184V+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R+p.T286A+p.I293V+p.E297K	Missense mutation	13	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, V60I, L74LI, K102E, K103R, V118VI, I135T, S162CY, M184V, L210W, R211K, T215Y, A272P, K277R, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44ED+p.K49KR+p.V60I+p.D67N+p.S68SI+p.V75ILM+p.V111VL+p.V118I+p.D121C+p.K122E+p.I135T+p.S156SFL+p.D177E+p.T200A+p.E203K+p.Q207E+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219R+p.V245K	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44ED, K49KR, V60I, D67N, S68SI, V75ILM, V111VL, V118I, D121C, K122E, I135T, S156SFL, D177E, T200A, E203K, Q207E, H208HY, L210W, R211K, T215Y, K219R, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44ED+p.V60I+p.A98G+p.K103N+p.V118I+p.I135T+p.V148VG+p.M184V+p.T200A+p.E203D+p.L210W+p.R211K+p.F214L+p.T215F	Missense mutation	69	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44ED, V60I, A98G, K103N, V118I, I135T, V148VG, M184V, T200A, E203D, L210W, R211K, F214L, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E53E*+p.K122KE+p.T131TP+p.I142V+p.K166IT+p.I178IM+p.R211KT+p.T215Y	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E53E*, K122KE, T131TP, I142V, K166IT, I178IM, R211KT, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E53EK+p.K101E+p.K103KN+p.V108VI+p.D123E+p.E138Q+p.D177DN+p.M184V+p.G190AS+p.Q207E+p.L210W+p.T215Y+p.A272P+p.A288AS+p.E297A	Missense mutation	67	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E53EK, K101E, K103KN, V108VI, D123E, E138Q, D177DN, M184V, G190AS, Q207E, L210W, T215Y, A272P, A288AS, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K101E+p.K103KN+p.D123E+p.E138Q+p.M184V+p.G190AS+p.Q207E+p.L210W+p.T215Y	Missense mutation	81	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K101E, K103KN, D123E, E138Q, M184V, G190AS, Q207E, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122E+p.E138G+p.D177E+p.I178L+p.L210W+p.R211K+p.T215Y+p.H221Y+p.F227L+p.M230L+p.V245M+p.I293V	Missense mutation	100	High-level resistance	[6]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122E, E138G, D177E, I178L, L210W, R211K, T215Y, H221Y, F227L, M230L, V245M, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122E+p.I135IL+p.K166KR+p.M184V+p.T200TA+p.Q207E+p.R211RK+p.T215Y+p.A272P+p.L283I+p.R284K+p.E297EQ	Missense mutation	11	Potential low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122E, I135IL, K166KR, M184V, T200TA, Q207E, R211RK, T215Y, A272P, L283I, R284K, E297EQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.D67N+p.V118I+p.N137NI+p.S162D+p.E169D+p.M184V+p.G190A+p.Q207QE+p.L210W+p.R211RK+p.T215Y	Missense mutation	23	Low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, D67N, V118I, N137NI, S162D, E169D, M184V, G190A, Q207QE, L210W, R211RK, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.D67G+p.S68G+p.L74LI+p.V118I+p.K122E+p.T139KR+p.M184V+p.H208Y+p.L210W+p.T215Y+p.K219N+p.K249Q+p.A272P+p.R284EV	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, D67G, S68G, L74LI, V118I, K122E, T139KR, M184V, H208Y, L210W, T215Y, K219N, K249Q, A272P, R284EV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.D67N+p.T69D+p.K102Q+p.K103N+p.V118I+p.K122E+p.I135T+p.K166KR+p.Y181C+p.G190A+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.L228H+p.H235HL	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, D67N, T69D, K102Q, K103N, V118I, K122E, I135T, K166KR, Y181C, G190A, Q207E, H208Y, L210W, R211K, T215Y, L228H, H235HL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.K49R+p.A62V+p.D67N+p.K70R+p.L74I+p.V75T+p.V118I+p.K122E+p.I135T+p.S162N+p.E169D+p.D177N+p.I178M+p.M184V+p.G196E+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219E+p.L228H+p.L283I+p.R284K+p.E297A+p.L301M	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, K49R, A62V, D67N, K70R, L74I, V75T, V118I, K122E, I135T, S162N, E169D, D177N, I178M, M184V, G196E, E203K, Q207E, L210W, R211K, T215Y, D218E, K219E, L228H, L283I, R284K, E297A, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.S68G+p.L74LI+p.K122E+p.D123DN+p.I142V+p.Q174K+p.I178L+p.M184V+p.T200K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	78	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, S68G, L74LI, K122E, D123DN, I142V, Q174K, I178L, M184V, T200K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.T69D+p.V75M+p.V118I+p.K122E+p.D123DN+p.F130FL+p.T131TP+p.I135T+p.E138ED+p.M184V+p.G196E+p.Q197K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	40	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, V60I, D67N, T69D, V75M, V118I, K122E, D123DN, F130FL, T131TP, I135T, E138ED, M184V, G196E, Q197K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.K49R+p.V60I+p.D67N+p.V118I+p.K122E+p.D123DN+p.I135T+p.T139M+p.T200A+p.L210W+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, K49R, V60I, D67N, V118I, K122E, D123DN, I135T, T139M, T200A, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.S68G+p.K122E+p.D123N+p.T139K+p.D177G+p.H208HY+p.L210W+p.R211K+p.T215F	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, S68G, K122E, D123N, T139K, D177G, H208HY, L210W, R211K, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.V60I+p.D67N+p.L74LI+p.A98S+p.L100LI+p.K101KR+p.K103S+p.V108VI+p.V118I+p.K122P+p.D123DN+p.I135T+p.D177G+p.M184V+p.G190GA+p.G196E+p.L210W+p.T215Y+p.K219N+p.L228HN+p.A272S+p.T286A+p.E297R	Missense mutation	54	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, V60I, D67N, L74LI, A98S, L100LI, K101KR, K103S, V108VI, V118I, K122P, D123DN, I135T, D177G, M184V, G190GA, G196E, L210W, T215Y, K219N, L228HN, A272S, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KE+p.A62V+p.K64R+p.D67E+p.T69A_SX+p.L74V+p.L100LI+p.K103KN+p.V111VI+p.K122E+p.I135T+p.T139TI+p.S162Y+p.E169EA+p.I178IL+p.Y181YC+p.M184MV+p.H208HL+p.L210W+p.R211K+p.T215Y+p.H221HY+p.L228H+p.V245E+p.A272P+p.K277R+p.V292VI	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KE, A62V, K64R, D67E, T69A_SX, L74V, L100LI, K103KN, V111VI, K122E, I135T, T139TI, S162Y, E169EA, I178IL, Y181YC, M184MV, H208HL, L210W, R211K, T215Y, H221HY, L228H, V245E, A272P, K277R, V292VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KE+p.E44EAD+p.D67N+p.K122E+p.S134SG+p.I135T+p.N136NI+p.I142V+p.G152GW+p.S162CY+p.M184V+p.G196GE+p.H208Y+p.L210W+p.R211RK+p.T215Y+p.E224D+p.L228LH+p.Q242QL+p.V245VE	Missense mutation	57	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KE, E44EAD, D67N, K122E, S134SG, I135T, N136NI, I142V, G152GW, S162CY, M184V, G196GE, H208Y, L210W, R211RK, T215Y, E224D, L228LH, Q242QL, V245VE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KE+p.V60I+p.D67N+p.T69E+p.K70R+p.K102W+p.V118I+p.D123E+p.S134SG+p.V148VG+p.I178M+p.Y188L+p.Q207A+p.L210W+p.R211K+p.T215F+p.K219Q+p.L228H+p.Q242H+p.V245T	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KE, V60I, D67N, T69E, K70R, K102W, V118I, D123E, S134SG, V148VG, I178M, Y188L, Q207A, L210W, R211K, T215F, K219Q, L228H, Q242H, V245T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KQ+p.E44ED+p.V60I+p.A98G+p.K103KN+p.V118I+p.I135T+p.T200A+p.E203D+p.L210W+p.R211K+p.F214L+p.T215F	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KQ, E44ED, V60I, A98G, K103KN, V118I, I135T, T200A, E203D, L210W, R211K, F214L, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.E44D+p.G45GW+p.V60I+p.D67N+p.T69DN+p.K70R+p.L74I+p.K102Q+p.K103N+p.D113DN+p.K122E+p.I135V+p.S156SLP+p.S162Y+p.D177E+p.I178L+p.V179I+p.Y181C+p.L210W+p.R211K+p.T215F+p.D218E+p.K219Q+p.K223Q+p.L228H	Missense mutation	58	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, E44D, G45GW, V60I, D67N, T69DN, K70R, L74I, K102Q, K103N, D113DN, K122E, I135V, S156SLP, S162Y, D177E, I178L, V179I, Y181C, L210W, R211K, T215F, D218E, K219Q, K223Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.E44D+p.L74IV+p.A98S+p.K103N+p.V108VI+p.V111I+p.V118I+p.K122P+p.D123E+p.I135M+p.D177E+p.V179I+p.M184V+p.G196E+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.L228R+p.D237DN	Missense mutation	21	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, E44D, L74IV, A98S, K103N, V108VI, V111I, V118I, K122P, D123E, I135M, D177E, V179I, M184V, G196E, Q207E, H208Y, L210W, R211K, T215Y, K219R, L228R, D237DN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.E44D+p.T69D+p.A98S+p.V106VL+p.V118I+p.D123E+p.I135V+p.I178M+p.V179I+p.Y188C+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.L228R+p.V245T+p.A272P+p.T286A+p.E297R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, E44D, T69D, A98S, V106VL, V118I, D123E, I135V, I178M, V179I, Y188C, E203K, H208Y, L210W, R211K, T215Y, K219R, K223E, L228R, V245T, A272P, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.D67N+p.L74V+p.V90I+p.K103KN+p.V108I+p.V118I+p.K122E+p.D123DN+p.I135T+p.N136NS+p.V148VG+p.P150PS+p.V179VL+p.I202V+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.H221Y+p.K223E+p.F227L	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, D67N, L74V, V90I, K103KN, V108I, V118I, K122E, D123DN, I135T, N136NS, V148VG, P150PS, V179VL, I202V, E203K, H208Y, L210W, R211K, T215Y, K219R, H221Y, K223E, F227L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.E44ED+p.V60I+p.D67G+p.T69D+p.V75M+p.H96HN+p.L100LI+p.K103KN+p.K104KR+p.V111VL+p.V118I+p.D121H+p.K122E+p.A129AV+p.I135V+p.N136NK+p.T139TK+p.I142V+p.V148VG+p.S162D+p.D177E+p.V179VI+p.M184MV+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R+p.V245E	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, E44ED, V60I, D67G, T69D, V75M, H96HN, L100LI, K103KN, K104KR, V111VL, V118I, D121H, K122E, A129AV, I135V, N136NK, T139TK, I142V, V148VG, S162D, D177E, V179VI, M184MV, L210W, R211K, T215Y, D218E, K219R, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.E44ED+p.V60I+p.D67N+p.T69D+p.K101X+p.V118I+p.K122E+p.M184MV+p.L210W+p.R211K+p.T215Y+p.F227FL+p.I257IT+p.K263KR+p.A272AP+p.G273GE+p.Q278QH	Missense mutation	32	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, E44ED, V60I, D67N, T69D, K101X, V118I, K122E, M184MV, L210W, R211K, T215Y, F227FL, I257IT, K263KR, A272AP, G273GE, Q278QH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.K49R+p.E53E*+p.T58TI+p.D67N+p.L74V+p.L100I+p.K103N+p.V118I+p.K122E+p.I135T+p.S162N+p.K173A+p.Q174K+p.D177EG+p.M184V+p.T200A+p.E203EK+p.R206RS+p.Q207E+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223KQ+p.V245E	Missense mutation	15	Low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, K49R, E53E*, T58TI, D67N, L74V, L100I, K103N, V118I, K122E, I135T, S162N, K173A, Q174K, D177EG, M184V, T200A, E203EK, R206RS, Q207E, H208HY, L210W, R211K, T215Y, K219N, K223KQ, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.K49R+p.V60I+p.D67N+p.R83K+p.K101E+p.D123E+p.V179I+p.M184V+p.G190S+p.R211K+p.T215F+p.M230L+p.D237E+p.A272P+p.T286A+p.E297K	Missense mutation	40	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, K49R, V60I, D67N, R83K, K101E, D123E, V179I, M184V, G190S, R211K, T215F, M230L, D237E, A272P, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43X+p.E44D+p.D67N+p.L74I+p.V75A+p.A98G+p.K103N+p.V118I+p.D177DN+p.M184V+p.G196E+p.E203D+p.H208Y+p.L210L*W+p.R211A+p.T215Y+p.K223Q	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43X, E44D, D67N, L74I, V75A, A98G, K103N, V118I, D177DN, M184V, G196E, E203D, H208Y, L210L*W, R211A, T215Y, K223Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K49KR+p.R72RT+p.K103KN+p.K122E+p.D123N+p.A129AS+p.I135T+p.N137NK+p.M184V+p.T215Y+p.V245MT	Missense mutation	15	Low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K49KR, R72RT, K103KN, K122E, D123N, A129AS, I135T, N137NK, M184V, T215Y, V245MT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K64KR+p.D67DN+p.V90I+p.K103N+p.V118VI+p.K122KE+p.I135T+p.T139K+p.I142V+p.S162A+p.Q174H+p.G196GE+p.T200A+p.Q207E+p.L210W+p.R211RK+p.T215F	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K64KR, D67DN, V90I, K103N, V118VI, K122KE, I135T, T139K, I142V, S162A, Q174H, G196GE, T200A, Q207E, L210W, R211RK, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K70R+p.V118I+p.M184V+p.K219Q	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K70R, V118I, M184V, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S48T+p.V60I+p.D67N+p.K70R+p.K103N+p.K122E+p.D123S+p.I135T+p.Q174K+p.M184V+p.R211K+p.F214L+p.T215TI+p.K219E+p.P225H+p.L228R	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S48T, V60I, D67N, K70R, K103N, K122E, D123S, I135T, Q174K, M184V, R211K, F214L, T215TI, K219E, P225H, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68G+p.K103N+p.K122E+p.V148VG+p.M184V+p.T200TA+p.L210F+p.T215Y+p.V245T	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68G, K103N, K122E, V148VG, M184V, T200TA, L210F, T215Y, V245T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.T69S_SS+p.R72RS+p.K73KQ+p.R83K+p.W88S+p.K122E+p.I135T+p.N137NI+p.E138EG+p.S162Y+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, T69S_SS, R72RS, K73KQ, R83K, W88S, K122E, I135T, N137NI, E138EG, S162Y, T200A, L210W, R211K, T215Y, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67G+p.T69N+p.K70R+p.A98S+p.K101Q+p.K122E+p.D123S+p.K126KR+p.I135T+p.I142V+p.A158AS+p.S162A+p.V179I+p.M184V+p.G190A+p.G196GR+p.T200TI+p.L210W+p.T215Y+p.K219E+p.V245E+p.P247PS+p.I293V+p.E297A	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67G, T69N, K70R, A98S, K101Q, K122E, D123S, K126KR, I135T, I142V, A158AS, S162A, V179I, M184V, G190A, G196GR, T200TI, L210W, T215Y, K219E, V245E, P247PS, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.K70R+p.K122E+p.D123S+p.I135V+p.K166R+p.D177E+p.T200I+p.E203D+p.R211K+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228H+p.V245M+p.A272P	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, K70R, K122E, D123S, I135V, K166R, D177E, T200I, E203D, R211K, F214L, T215F, D218E, K219Q, L228H, V245M, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.K70R+p.V75M+p.K101E+p.D123E+p.M184V+p.G190S+p.G196E+p.Q207H+p.T215F+p.K219E+p.A272P+p.Q278H+p.E297K	Missense mutation	33	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, K70R, V75M, K101E, D123E, M184V, G190S, G196E, Q207H, T215F, K219E, A272P, Q278H, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.L74V+p.K103N+p.V118I+p.K122E+p.I135V+p.K154KE+p.I178M+p.M184V+p.T200A+p.E203K+p.L210W+p.R211K+p.T215Y+p.K219A+p.K223Q+p.V245M	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, L74V, K103N, V118I, K122E, I135V, K154KE, I178M, M184V, T200A, E203K, L210W, R211K, T215Y, K219A, K223Q, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.V75MT+p.K103N+p.V118I+p.K122E+p.I135V+p.E138EAD+p.Y144YS+p.P150PHQ+p.V189VL+p.T200I+p.F214L+p.T215F+p.K219Q+p.L228H	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, T69D, K70R, V75MT, K103N, V118I, K122E, I135V, E138EAD, Y144YS, P150PHQ, V189VL, T200I, F214L, T215F, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.K64KR+p.D67DN+p.R83K+p.V118I+p.K122E+p.I135T+p.S162Y+p.M184V+p.Y188L+p.L210W+p.F214FL+p.T215Y+p.K219N+p.L228LH+p.Q242QH+p.V245E+p.T286A	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, K64KR, D67DN, R83K, V118I, K122E, I135T, S162Y, M184V, Y188L, L210W, F214FL, T215Y, K219N, L228LH, Q242QH, V245E, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60VI+p.K64KR+p.D67DN+p.A98G+p.V108I+p.D121C+p.K122E+p.I135V+p.D177E+p.Y181YC+p.M184V+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223KT+p.L228HR+p.K281KR+p.I293IV+p.E297KR	Missense mutation	75	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60VI, K64KR, D67DN, A98G, V108I, D121C, K122E, I135V, D177E, Y181YC, M184V, L210W, R211K, T215Y, K219N, K223KT, L228HR, K281KR, I293IV, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V75M+p.F77L+p.K101E+p.K122E+p.S162C+p.E169D+p.Q182S+p.M184V+p.Y188L+p.T200A+p.I202V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228R+p.I257L+p.A272P+p.I293V+p.E297K	Missense mutation	64	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V75M, F77L, K101E, K122E, S162C, E169D, Q182S, M184V, Y188L, T200A, I202V, Q207E, L210W, R211K, T215Y, L228R, I257L, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.A62AV+p.K103KN+p.V111VL+p.D123E+p.I135T+p.I159IM+p.D177E+p.M184V+p.L210LW+p.R211K+p.T215Y+p.L228LR	Missense mutation	10	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, A62AV, K103KN, V111VL, D123E, I135T, I159IM, D177E, M184V, L210LW, R211K, T215Y, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.A98S+p.K103N+p.V118VI+p.K122E+p.D177E+p.M184V+p.Q207E+p.R211K+p.T215Y+p.L228H+p.M230L+p.D250E+p.A272P+p.I274V	Missense mutation	38	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, A98S, K103N, V118VI, K122E, D177E, M184V, Q207E, R211K, T215Y, L228H, M230L, D250E, A272P, I274V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.E44ED+p.T69AD+p.A98AS+p.K101PQ+p.K103KN+p.V118VI+p.K122E+p.D123DN+p.Y144Y*+p.R172RK+p.D192N+p.E203EK+p.Q207QE+p.L210LW+p.R211RK+p.F214FL+p.T215X+p.K219KR+p.K223KE+p.L228LR	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, E44ED, T69AD, A98AS, K101PQ, K103KN, V118VI, K122E, D123DN, Y144Y*, R172RK, D192N, E203EK, Q207QE, L210LW, R211RK, F214FL, T215X, K219KR, K223KE, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P14PS+p.K20KR+p.V35I+p.K46Q+p.A62V+p.K65R+p.S68G+p.T69K+p.K70R+p.V75I+p.F77L+p.Y115F+p.F116Y+p.V118I+p.K122E+p.Q151M+p.R172K+p.D177E+p.M184V+p.Y188L+p.G196E+p.T200TI+p.I202V+p.F214L+p.T215TI+p.K219H+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P14PS, K20KR, V35I, K46Q, A62V, K65R, S68G, T69K, K70R, V75I, F77L, Y115F, F116Y, V118I, K122E, Q151M, R172K, D177E, M184V, Y188L, G196E, T200TI, I202V, F214L, T215TI, K219H, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P14PS+p.K20R+p.M41L+p.K43E+p.V60I+p.D67X+p.T69TI+p.L80LF+p.T84TI+p.V118I+p.D121DN+p.K122E+p.I135M+p.M184V+p.G196GE+p.L210W+p.T215Y	Missense mutation	83	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P14PS, K20R, M41L, K43E, V60I, D67X, T69TI, L80LF, T84TI, V118I, D121DN, K122E, I135M, M184V, G196GE, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1H+p.K20R+p.V35L+p.T39TA+p.V60I+p.D67N+p.T69D+p.K70R+p.L74V+p.R83K+p.K101Q+p.I135V+p.I178IT+p.V179VI+p.Y181C+p.G190A+p.I195IT+p.E204ED+p.R211T+p.T215Y+p.D218E+p.K219Q+p.L228H	Missense mutation	22	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1H, K20R, V35L, T39TA, V60I, D67N, T69D, K70R, L74V, R83K, K101Q, I135V, I178IT, V179VI, Y181C, G190A, I195IT, E204ED, R211T, T215Y, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K20R+p.D67N+p.S68N+p.K70G+p.K103N+p.V106VA+p.F116Y+p.K122E+p.I135T+p.E138EA+p.Q151M+p.I167IV+p.V179I+p.I202V+p.R211K+p.T215Y+p.L228LR+p.I270IM+p.T286A+p.E297K	Missense mutation	13	Potential low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K20R, D67N, S68N, K70G, K103N, V106VA, F116Y, K122E, I135T, E138EA, Q151M, I167IV, V179I, I202V, R211K, T215Y, L228LR, I270IM, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.M41ML+p.K64H+p.D67G+p.T69DN+p.K70R+p.L74I+p.V118I+p.K122E+p.I135T+p.M184V+p.T200S+p.F214L+p.T215F+p.D218E+p.K219Q+p.T286TA	Missense mutation	18	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, M41ML, K64H, D67G, T69DN, K70R, L74I, V118I, K122E, I135T, M184V, T200S, F214L, T215F, D218E, K219Q, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.V35I+p.S48T+p.V60I+p.D67N+p.K70R+p.D121H+p.K122E+p.K166R+p.D177E+p.G196E+p.I202V+p.T215F+p.K219E	Missense mutation	51	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, V35I, S48T, V60I, D67N, K70R, D121H, K122E, K166R, D177E, G196E, I202V, T215F, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4PS+p.K20KR+p.E28K+p.K32E+p.M41L+p.K43Q+p.E44D+p.V60I+p.K64KR+p.K66KR+p.D67N+p.L74LV+p.W88C+p.A98G+p.K101E+p.V108I+p.V118I+p.K122E+p.I135T+p.E138EK+p.D177DG+p.Y181C+p.G190A+p.I195K+p.G196E+p.T200A+p.E203K+p.Q207D+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.E224ED+p.L228S	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4PS, K20KR, E28K, K32E, M41L, K43Q, E44D, V60I, K64KR, K66KR, D67N, L74LV, W88C, A98G, K101E, V108I, V118I, K122E, I135T, E138EK, D177DG, Y181C, G190A, I195K, G196E, T200A, E203K, Q207D, L210W, R211K, T215Y, K219R, K223E, E224ED, L228S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.E6EK+p.K20R+p.V35VI+p.T39TA+p.M41L+p.K43N+p.E44ED+p.D67N+p.L74LV+p.K101Q+p.V118I+p.K122E+p.I135T+p.S162SC+p.E169ED+p.Q174K+p.N175Y+p.I178IL+p.I195IV+p.G196E+p.T200A+p.I202IT+p.E203D+p.Q207K+p.H208HY+p.L210W+p.R211A+p.W212WQR+p.T215Y+p.K219KR+p.K223T+p.L228LH+p.E248D+p.A272P+p.T286A+p.L289LF+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, E6EK, K20R, V35VI, T39TA, M41L, K43N, E44ED, D67N, L74LV, K101Q, V118I, K122E, I135T, S162SC, E169ED, Q174K, N175Y, I178IL, I195IV, G196E, T200A, I202IT, E203D, Q207K, H208HY, L210W, R211A, W212WQR, T215Y, K219KR, K223T, L228LH, E248D, A272P, T286A, L289LF, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K11KT+p.K20R+p.E28K+p.M41L+p.K43N+p.E44D+p.V60VI+p.D67N+p.T69D+p.A98S+p.K101KE+p.V118I+p.I135V+p.V179VI+p.M184V+p.Y188L+p.G196E+p.E203D+p.L210W+p.R211K+p.F214FL+p.T215Y+p.D237E+p.E248D+p.E297EK+p.L303LP	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K11KT, K20R, E28K, M41L, K43N, E44D, V60VI, D67N, T69D, A98S, K101KE, V118I, I135V, V179VI, M184V, Y188L, G196E, E203D, L210W, R211K, F214FL, T215Y, D237E, E248D, E297EK, L303LP.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K20R+p.M41L+p.A98G+p.K102KR+p.K103N+p.Q174H+p.D177E+p.I178L+p.M184MV+p.R211K+p.T215Y+p.D237DE	Missense mutation	30	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K20R, M41L, A98G, K102KR, K103N, Q174H, D177E, I178L, M184MV, R211K, T215Y, D237DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K20R+p.T39TA+p.M41L+p.V60I+p.K64KR+p.D67N+p.K70R+p.V118VI+p.I135T+p.M184V+p.T200IL+p.T215Y+p.K219E+p.P225H+p.L228H+p.Q242QH+p.V261VA+p.Q278QH+p.E297PQ	Missense mutation	41	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K20R, T39TA, M41L, V60I, K64KR, D67N, K70R, V118VI, I135T, M184V, T200IL, T215Y, K219E, P225H, L228H, Q242QH, V261VA, Q278QH, E297PQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.M41L+p.E44ED+p.V60VI+p.D67N+p.R83K+p.K101P+p.K103S+p.V118I+p.K122E+p.D123N+p.I135V+p.Q197E+p.I202V+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, M41L, E44ED, V60VI, D67N, R83K, K101P, K103S, V118I, K122E, D123N, I135V, Q197E, I202V, L210W, R211K, T215Y, K219N, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.T27TS+p.M41L+p.D67N+p.K70R+p.R72RK+p.A98G+p.K104R+p.T139K+p.V179I+p.M184V+p.G190A+p.R211RK+p.T215F+p.D218E+p.K219Q	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, T27TS, M41L, D67N, K70R, R72RK, A98G, K104R, T139K, V179I, M184V, G190A, R211RK, T215F, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.T7TP+p.M16ML+p.V21VI+p.V35I+p.M41ML+p.K43Q+p.E44D+p.D67N+p.T69D+p.A98G+p.V111I+p.D123E+p.Y181YD+p.M184V+p.G196GE+p.T200TA+p.E203KR+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.L228LF+p.M230MV	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, T7TP, M16ML, V21VI, V35I, M41ML, K43Q, E44D, D67N, T69D, A98G, V111I, D123E, Y181YD, M184V, G196GE, T200TA, E203KR, Q207D, H208Y, L210W, R211K, T215Y, K219R, K223Q, L228LF, M230MV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.V35I+p.T39E+p.M41L+p.V60I+p.D67N+p.L74I+p.I135R+p.A158S+p.V179E+p.Y181C+p.M184V+p.G196E+p.T200TA+p.I202IV+p.Q207A+p.H208HY+p.R211K+p.T215F+p.D218DE+p.K219W+p.L228H	Missense mutation	20	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, V35I, T39E, M41L, V60I, D67N, L74I, I135R, A158S, V179E, Y181C, M184V, G196E, T200TA, I202IV, Q207A, H208HY, R211K, T215F, D218DE, K219W, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4ST+p.V35I+p.T39A+p.M41L+p.E44D+p.V118I+p.K122E+p.T200TA+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	78	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4ST, V35I, T39A, M41L, E44D, V118I, K122E, T200TA, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4T+p.E6K+p.V35VIL+p.M41L+p.K43M+p.D67G+p.S68G+p.L74V+p.V75VA+p.K103N+p.V118I+p.K122E+p.S162D+p.K166KR+p.Q174K+p.Y181C+p.M184V+p.T200A+p.E203K+p.L210W+p.T215Y+p.K219N+p.K223E+p.L228LH+p.Q242QH+p.V245K+p.T286A+p.E297EA	Missense mutation	16	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4T, E6K, V35VIL, M41L, K43M, D67G, S68G, L74V, V75VA, K103N, V118I, K122E, S162D, K166KR, Q174K, Y181C, M184V, T200A, E203K, L210W, T215Y, K219N, K223E, L228LH, Q242QH, V245K, T286A, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4T+p.T39TA+p.M41L+p.K43KR+p.E44ED+p.D67G+p.S68G+p.T69TI+p.L74V+p.A98AS+p.K103N+p.V118I+p.I135T+p.I142IV+p.K173Q+p.Y181YC+p.M184V+p.G196E+p.T200A+p.E203V+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.K219NS+p.K223E+p.F227L+p.L228HR	Missense mutation	46	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4T, T39TA, M41L, K43KR, E44ED, D67G, S68G, T69TI, L74V, A98AS, K103N, V118I, I135T, I142IV, K173Q, Y181YC, M184V, G196E, T200A, E203V, Q207E, L210W, R211RK, T215Y, K219NS, K223E, F227L, L228HR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4T+p.V35M+p.M41L+p.K43EQ+p.V60VI+p.D67N+p.T69N+p.K70R+p.A98S+p.K104N+p.K122E+p.D123N+p.I135V+p.S162SN+p.F171FL+p.M184V+p.G190A+p.Q207E+p.T215F+p.K219Q+p.L228HQ+p.T286TA	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4T, V35M, M41L, K43EQ, V60VI, D67N, T69N, K70R, A98S, K104N, K122E, D123N, I135V, S162SN, F171FL, M184V, G190A, Q207E, T215F, K219Q, L228HQ, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9PS+p.M41L+p.K43E+p.E44A+p.D67N+p.V75M+p.F77L+p.V90I+p.K103N+p.V118I+p.K122E+p.D123E+p.I135T+p.S162Y+p.M184V+p.G196E+p.L210W+p.T215Y+p.K219Q	Missense mutation	18	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9PS, M41L, K43E, E44A, D67N, V75M, F77L, V90I, K103N, V118I, K122E, D123E, I135T, S162Y, M184V, G196E, L210W, T215Y, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q23QR+p.V35VA+p.M41L+p.V60I+p.D67N+p.K70KR+p.L74LIV+p.V75VL+p.K101Q+p.K103N+p.V108I+p.D123E+p.I135T+p.I178M+p.M184V+p.L210W+p.R211K+p.T215Y+p.K219Q+p.L228H+p.K249T+p.A272P+p.V276T+p.E291D+p.E297K	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q23QR, V35VA, M41L, V60I, D67N, K70KR, L74LIV, V75VL, K101Q, K103N, V108I, D123E, I135T, I178M, M184V, L210W, R211K, T215Y, K219Q, L228H, K249T, A272P, V276T, E291D, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.E6K+p.T39TA+p.M41L+p.K43X+p.E44ED+p.K64R+p.D67N+p.V90I+p.K103KN+p.K122E+p.I135T+p.V179I+p.M184IV+p.T200X+p.E203EQ+p.Q207D+p.L210W+p.R211K+p.T215Y+p.R284K+p.T286A+p.A288T	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, E6K, T39TA, M41L, K43X, E44ED, K64R, D67N, V90I, K103KN, K122E, I135T, V179I, M184IV, T200X, E203EQ, Q207D, L210W, R211K, T215Y, R284K, T286A, A288T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.K20R+p.M41L+p.K43E+p.E44A+p.L74V+p.A98G+p.L100I+p.K103N+p.I135T+p.M184V+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228R	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, K20R, M41L, K43E, E44A, L74V, A98G, L100I, K103N, I135T, M184V, E203K, Q207E, L210W, R211K, T215Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.T39N+p.M41L+p.K43E+p.E44A+p.D67N+p.T69TA+p.L74I+p.V75VIM+p.A98G+p.V106I+p.D123E+p.I142V+p.V179I+p.M184V+p.G190S+p.T200A+p.L210W+p.T215Y+p.K219E+p.W229WR+p.I257L+p.T286A+p.V292I+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, T39N, M41L, K43E, E44A, D67N, T69TA, L74I, V75VIM, A98G, V106I, D123E, I142V, V179I, M184V, G190S, T200A, L210W, T215Y, K219E, W229WR, I257L, T286A, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.K20R+p.M41L+p.K104KR+p.D113DE+p.I135T+p.S162SG+p.M184V+p.G196GE+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K223KQ+p.L228LR+p.K238KR+p.V276VI+p.Q278QR+p.T286TA	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, K20R, M41L, K104KR, D113DE, I135T, S162SG, M184V, G196GE, E203K, Q207E, L210W, R211K, T215Y, K223KQ, L228LR, K238KR, V276VI, Q278QR, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.K20R+p.V35I+p.M41L+p.K43N+p.D67N+p.L74V+p.A98G+p.K103N+p.D123E+p.T139K+p.D177E+p.Y181C+p.M184V+p.T200A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221CR+p.A272P+p.E297A	Missense mutation	58	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, K20R, V35I, M41L, K43N, D67N, L74V, A98G, K103N, D123E, T139K, D177E, Y181C, M184V, T200A, H208Y, L210W, R211K, T215Y, H221CR, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.T7TP+p.V35E+p.T39A+p.M41L+p.K122E+p.D123N+p.I135T+p.E138A+p.I142V+p.S162CY+p.V179I+p.Y181I+p.M184V+p.H208HY+p.L210W+p.R211RG+p.T215Y+p.H221C+p.L228R	Missense mutation	82	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, T7TP, V35E, T39A, M41L, K122E, D123N, I135T, E138A, I142V, S162CY, V179I, Y181I, M184V, H208HY, L210W, R211RG, T215Y, H221C, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SG+p.V35M+p.K70KE+p.R83K+p.K103KN+p.A158S+p.E169D+p.Y181YC+p.T200I+p.L210W+p.R211K+p.T215Y+p.A272P	Missense mutation	26	Low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SG, V35M, K70KE, R83K, K103KN, A158S, E169D, Y181YC, T200I, L210W, R211K, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.M41L+p.K43E+p.K49KR+p.K101H+p.K122E+p.I142V+p.E169D+p.Q174QK+p.I178L+p.H208Y+p.L210W+p.R211K+p.T215Y+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, M41L, K43E, K49KR, K101H, K122E, I142V, E169D, Q174QK, I178L, H208Y, L210W, R211K, T215Y, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27TI+p.E29EG+p.V35I+p.T39A+p.M41L+p.K43E+p.V60I+p.K64R+p.D67N+p.L74I+p.F77FL+p.A98S+p.K103N+p.V108I+p.V118I+p.K122E+p.D177G+p.M184V+p.G196E+p.E203K+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q+p.K223Q+p.L228H	Missense mutation	24	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27TI, E29EG, V35I, T39A, M41L, K43E, V60I, K64R, D67N, L74I, F77FL, A98S, K103N, V108I, V118I, K122E, D177G, M184V, G196E, E203K, L210W, R211K, T215Y, D218E, K219Q, K223Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27TP+p.K32KR+p.V35I+p.M41L+p.E44D+p.V60I+p.K64KR+p.D67N+p.K70R+p.L74I+p.K101H+p.V111I+p.S162D+p.K173RT+p.Q174E+p.I178L+p.V179I+p.M184V+p.G190A+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27TP, K32KR, V35I, M41L, E44D, V60I, K64KR, D67N, K70R, L74I, K101H, V111I, S162D, K173RT, Q174E, I178L, V179I, M184V, G190A, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40EK+p.M41L+p.K43E+p.V60I+p.D67N+p.V75M+p.A98S+p.K101Q+p.K103KN+p.V118I+p.D121DH+p.K122KE+p.D123DE+p.I142V+p.V179VI+p.Y181C+p.V189VL+p.G190A+p.L210W+p.R211T+p.T215Y+p.H221Y	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40EK, M41L, K43E, V60I, D67N, V75M, A98S, K101Q, K103KN, V118I, D121DH, K122KE, D123DE, I142V, V179VI, Y181C, V189VL, G190A, L210W, R211T, T215Y, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40F+p.M41L+p.K43E+p.T58X+p.T69D+p.V75M+p.K82R+p.R83K+p.V90I+p.H96HN+p.A98G+p.K101Q+p.V108I+p.V118I+p.K122E+p.R125RG+p.I142V+p.Y144Y*+p.S162C+p.R172RK+p.Y181C+p.V189I+p.G190A+p.H208Y+p.L210W+p.R211RK+p.T215Y+p.K223Q+p.L228R	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40F, M41L, K43E, T58X, T69D, V75M, K82R, R83K, V90I, H96HN, A98G, K101Q, V108I, V118I, K122E, R125RG, I142V, Y144Y*, S162C, R172RK, Y181C, V189I, G190A, H208Y, L210W, R211RK, T215Y, K223Q, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E53D+p.D67N+p.R83K+p.H96HN+p.K101E+p.T139TR+p.G152GR+p.S162Y+p.G190A+p.R211K+p.T215F+p.H235HL+p.V245T	Missense mutation	93	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E53D, D67N, R83K, H96HN, K101E, T139TR, G152GR, S162Y, G190A, R211K, T215F, H235HL, V245T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K64H+p.D67N+p.T69N+p.K70R+p.D123E+p.M184V+p.T200TA+p.F214L+p.D218E+p.K219Q+p.L228HQ	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K64H, D67N, T69N, K70R, D123E, M184V, T200TA, F214L, D218E, K219Q, L228HQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.K70R+p.V75L+p.K103N+p.K122E+p.I135T+p.I178IM+p.M184V+p.I195IL+p.G196E+p.T200TA+p.L210W+p.T215Y+p.K219E+p.L228N+p.E248N+p.D250E+p.T286A+p.A288S+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, K70R, V75L, K103N, K122E, I135T, I178IM, M184V, I195IL, G196E, T200TA, L210W, T215Y, K219E, L228N, E248N, D250E, T286A, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.T69D+p.R83RS+p.V111VL+p.V118I+p.D121H+p.K122E+p.N137NK+p.D177E+p.G196E+p.E203EK+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, T69D, R83RS, V111VL, V118I, D121H, K122E, N137NK, D177E, G196E, E203EK, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.T69TA+p.V90VI+p.K103N+p.V118I+p.K122E+p.I135V+p.I142V+p.M184V+p.L210W+p.R211K+p.T215Y+p.Y232H+p.V261VG+p.A272S+p.K277R+p.Q278E+p.I293V+p.E297K	Missense mutation	12	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, T69TA, V90VI, K103N, V118I, K122E, I135V, I142V, M184V, L210W, R211K, T215Y, Y232H, V261VG, A272S, K277R, Q278E, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D76DN+p.E79ED+p.E89EK+p.H96HN+p.V106I+p.I135T+p.K173N+p.Q174K+p.V179E+p.Y188L+p.T200A+p.E203EK+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221Y+p.D237E+p.V245Q	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D76DN, E79ED, E89EK, H96HN, V106I, I135T, K173N, Q174K, V179E, Y188L, T200A, E203EK, H208Y, L210W, R211K, T215Y, H221Y, D237E, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.D67E+p.S68G+p.L74I+p.V75M+p.V108I+p.V118I+p.K122E+p.I135T+p.T165I+p.M184V+p.V189I+p.G190A+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K223Q+p.V245K	Missense mutation	50	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, D67E, S68G, L74I, V75M, V108I, V118I, K122E, I135T, T165I, M184V, V189I, G190A, E203K, Q207E, L210W, R211K, T215Y, K223Q, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.D67N+p.T69D+p.V75M+p.K103N+p.V106A+p.K122E+p.F130FL+p.S162C+p.E169D+p.I178L+p.G196E+p.T200TA+p.E203EK+p.Q207E+p.L210W+p.R211K+p.T215Y+p.F227L	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, D67N, T69D, V75M, K103N, V106A, K122E, F130FL, S162C, E169D, I178L, G196E, T200TA, E203EK, Q207E, L210W, R211K, T215Y, F227L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.T69D+p.A98G+p.V118I+p.K122E+p.I142V+p.S162Y+p.I178F+p.V179I+p.M184V+p.G190GA+p.T200TA+p.E203A+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.L228R	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, T69D, A98G, V118I, K122E, I142V, S162Y, I178F, V179I, M184V, G190GA, T200TA, E203A, Q207E, H208Y, L210W, R211K, T215Y, K219R, K223Q, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.T69D+p.K103KN+p.V106VA+p.V118I+p.K122E+p.I142V+p.S162FY+p.Y181YC+p.M184V+p.E203K+p.Q207E+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.E224K+p.L228LH+p.Q242K+p.A288AS+p.E297K	Missense mutation	11	Potential low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, T69D, K103KN, V106VA, V118I, K122E, I142V, S162FY, Y181YC, M184V, E203K, Q207E, H208HY, L210W, R211K, T215Y, K219R, K223E, E224K, L228LH, Q242K, A288AS, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.T69D+p.L74V+p.K103N+p.V118I+p.K122E+p.I135IMT+p.I142V+p.S162Y+p.Y181C+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223E+p.E224K+p.F227FL+p.L228H+p.Q242K+p.A288S+p.E297K	Missense mutation	16	Low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, T69D, L74V, K103N, V118I, K122E, I135IMT, I142V, S162Y, Y181C, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219R, K223E, E224K, F227FL, L228H, Q242K, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.V60I+p.D67N+p.T69D+p.A98AS+p.V118I+p.D121H+p.K122E+p.I142V+p.D177E+p.L210W+p.R211N+p.T215Y+p.K219KR	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, V60I, D67N, T69D, A98AS, V118I, D121H, K122E, I142V, D177E, L210W, R211N, T215Y, K219KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43E+p.E44A+p.D67N+p.W88C+p.K101Q+p.V106I+p.V118I+p.K122E+p.I135V+p.S162A+p.E169EG+p.K173R+p.Q174K+p.V179VI+p.I195K+p.G196E+p.R199RK+p.Q207R+p.H208Y+p.L210W+p.R211E+p.T215Y+p.K219R+p.K223E+p.L228H+p.A272P	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43E, E44A, D67N, W88C, K101Q, V106I, V118I, K122E, I135V, S162A, E169EG, K173R, Q174K, V179VI, I195K, G196E, R199RK, Q207R, H208Y, L210W, R211E, T215Y, K219R, K223E, L228H, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.T69D+p.V75M+p.F77FL+p.K101H+p.V118I+p.K122E+p.I135T+p.S162Y+p.I167IL+p.M184V+p.Q207QK+p.H208X+p.L210W+p.R211X+p.W212WC+p.T215Y+p.K219KN+p.K223Q+p.L228LI+p.Q242L	Missense mutation	38	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43E, E44A, V60I, D67N, T69D, V75M, F77FL, K101H, V118I, K122E, I135T, S162Y, I167IL, M184V, Q207QK, H208X, L210W, R211X, W212WC, T215Y, K219KN, K223Q, L228LI, Q242L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43E+p.E44D+p.D67G+p.S68G+p.V75M+p.W88C+p.V90I+p.K101E+p.V111VL+p.V118I+p.K122E+p.S134SG+p.I135T+p.E138A+p.I178M+p.V179VI+p.G190A+p.T200TA+p.E203EK+p.Q207Q*+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K223Q	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43E, E44D, D67G, S68G, V75M, W88C, V90I, K101E, V111VL, V118I, K122E, S134SG, I135T, E138A, I178M, V179VI, G190A, T200TA, E203EK, Q207Q*, H208HY, L210W, R211K, T215Y, K223Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43X+p.E44D+p.S68G+p.L74V+p.A98G+p.V111VG+p.V118I+p.D123E+p.I135T+p.E138G+p.T139TA+p.I178L+p.V179DE+p.Y188L+p.G196E+p.T200TA+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223E+p.E224ED+p.V245VA+p.A272P+p.A288S	Missense mutation	45	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43X, E44D, S68G, L74V, A98G, V111VG, V118I, D123E, I135T, E138G, T139TA, I178L, V179DE, Y188L, G196E, T200TA, E203K, H208Y, L210W, R211K, T215Y, K219N, K223E, E224ED, V245VA, A272P, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K64R+p.L74V+p.K102Q+p.V118I+p.K122E+p.I135T+p.I142V+p.K166R+p.Q174R+p.M184V+p.T200A+p.L210W+p.F214L+p.T215Y+p.V245N	Missense mutation	13	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K64R, L74V, K102Q, V118I, K122E, I135T, I142V, K166R, Q174R, M184V, T200A, L210W, F214L, T215Y, V245N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.V60I+p.D67N+p.K70R+p.V118VI+p.D123E+p.S134SC+p.I135T+p.P150PQ+p.K166Q+p.D177E+p.V179I+p.G190GA+p.I202V+p.L210W+p.T215Y+p.K219E+p.E224EV+p.L228H+p.D237DY+p.V245Q	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, V60I, D67N, K70R, V118VI, D123E, S134SC, I135T, P150PQ, K166Q, D177E, V179I, G190GA, I202V, L210W, T215Y, K219E, E224EV, L228H, D237DY, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.V75M+p.K102L+p.V118I+p.K122E+p.I135T+p.P157PS+p.V179I+p.Y188L+p.G196E+p.T200I+p.H208Y+p.L210F+p.R211K+p.T215Y+p.D218E+p.K219Q+p.L228H+p.V245M	Missense mutation	48	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, V60I, D67N, T69D, K70R, V75M, K102L, V118I, K122E, I135T, P157PS, V179I, Y188L, G196E, T200I, H208Y, L210F, R211K, T215Y, D218E, K219Q, L228H, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39AG+p.E40EK+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.K103N+p.K122E+p.D123N+p.I135T+p.S162Y+p.E203D+p.Q207E+p.T215F+p.K219Q+p.L228H+p.V245T	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39AG, E40EK, M41L, V60I, D67N, T69D, K70R, K103N, K122E, D123N, I135T, S162Y, E203D, Q207E, T215F, K219Q, L228H, V245T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39AS+p.M41L+p.K43N+p.E44D+p.D67N+p.T69D+p.L74V+p.K101E+p.K102KR+p.V118I+p.K122E+p.I135T+p.E138E*+p.P150PS+p.V179I+p.Y181C+p.G190A+p.G196E+p.E203K+p.L210W+p.R211K+p.T215Y+p.K223Q+p.H235HL	Missense mutation	54	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39AS, M41L, K43N, E44D, D67N, T69D, L74V, K101E, K102KR, V118I, K122E, I135T, E138E*, P150PS, V179I, Y181C, G190A, G196E, E203K, L210W, R211K, T215Y, K223Q, H235HL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.M41L+p.E44D+p.D67N+p.K70R+p.K103N+p.V118I+p.K122E+p.D123N+p.I135T+p.V179VI+p.M184V+p.T200A+p.F214L+p.T215F+p.K219Q	Missense mutation	41	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, M41L, E44D, D67N, K70R, K103N, V118I, K122E, D123N, I135T, V179VI, M184V, T200A, F214L, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.M41L+p.E44D+p.D67NS+p.S68SN+p.K70GR+p.K103N+p.V111VIM+p.V118I+p.K122E+p.D123N+p.I135T+p.V179I+p.M184V+p.D192DN+p.T200A+p.F214L+p.T215F+p.K219Q	Missense mutation	28	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, M41L, E44D, D67NS, S68SN, K70GR, K103N, V111VIM, V118I, K122E, D123N, I135T, V179I, M184V, D192DN, T200A, F214L, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.M41L+p.E44D+p.K64R+p.D67N+p.L74V+p.L100I+p.K103N+p.V106I+p.V118I+p.I135T+p.I142V+p.V179F+p.Y181C+p.M184V+p.G196E+p.T200A+p.E203K+p.Q207E+p.H208Y+p.L210W+p.T215Y+p.K219N+p.K223E+p.F227L	Missense mutation	37	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, M41L, E44D, K64R, D67N, L74V, L100I, K103N, V106I, V118I, I135T, I142V, V179F, Y181C, M184V, G196E, T200A, E203K, Q207E, H208Y, L210W, T215Y, K219N, K223E, F227L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.M41L+p.E44D+p.V60I+p.S68G+p.A98G+p.V118I+p.K122E+p.I135T+p.M184V+p.G196E+p.T200TA+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	22	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, M41L, E44D, V60I, S68G, A98G, V118I, K122E, I135T, M184V, G196E, T200TA, E203K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.S48T+p.V60I+p.L74V+p.K103N+p.Y115F+p.D121H+p.K122E+p.I135T+p.K173T+p.Q174K+p.D177E+p.I178L+p.Y181C+p.M184V+p.T200A+p.I202IV+p.Q207E+p.V245Q	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, S48T, V60I, L74V, K103N, Y115F, D121H, K122E, I135T, K173T, Q174K, D177E, I178L, Y181C, M184V, T200A, I202IV, Q207E, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39K+p.E40EK+p.M41L+p.E42EG+p.K43E+p.K49R+p.K122E+p.S134SG+p.G152GW+p.E169K+p.K173E+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.L228LH	Missense mutation	44	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39K, E40EK, M41L, E42EG, K43E, K49R, K122E, S134SG, G152GW, E169K, K173E, Q207E, L210W, R211RK, T215Y, L228LH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39K+p.E40F+p.M41L+p.K43E+p.D67N+p.L74V+p.I94L+p.K101N+p.V118I+p.K122E+p.D123S+p.E138X+p.T139TS+p.S162A+p.Y181C+p.G190S+p.L210W+p.R211K+p.T215Y+p.K219N	Missense mutation	44	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39K, E40F, M41L, K43E, D67N, L74V, I94L, K101N, V118I, K122E, D123S, E138X, T139TS, S162A, Y181C, G190S, L210W, R211K, T215Y, K219N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39K+p.M41L+p.K43E+p.E44A+p.V90I+p.K101I+p.I142V+p.S162A+p.Y181C+p.M184V+p.G190S+p.Q197K+p.T200A+p.Q207E+p.T215Y+p.L228R	Missense mutation	14	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39K, M41L, K43E, E44A, V90I, K101I, I142V, S162A, Y181C, M184V, G190S, Q197K, T200A, Q207E, T215Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39K+p.M41L+p.K43E+p.V60VI+p.D67N+p.T69X+p.V75L+p.A98G+p.K101E+p.V118I+p.I135T+p.Y181C+p.G190A+p.E203D+p.Q207D+p.H208HY+p.L210W+p.R211K+p.T215Y+p.H221HY+p.L228LH+p.V245EKQ+p.A288S	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39K, M41L, K43E, V60VI, D67N, T69X, V75L, A98G, K101E, V118I, I135T, Y181C, G190A, E203D, Q207D, H208HY, L210W, R211K, T215Y, H221HY, L228LH, V245EKQ, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39M+p.M41L+p.D67N+p.T69D+p.K70R+p.L74V+p.V75T+p.K101EQ+p.K102KR+p.V118I+p.K122E+p.S162D+p.F171Y+p.K173T+p.Q174K+p.D177E+p.I178L+p.Y181C+p.G190A+p.E203K+p.Q207E+p.H208Y+p.R211A+p.F214L+p.T215F+p.K219Q+p.K223Q+p.L228H+p.V245Q	Missense mutation	12	Potential low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39M, M41L, D67N, T69D, K70R, L74V, V75T, K101EQ, K102KR, V118I, K122E, S162D, F171Y, K173T, Q174K, D177E, I178L, Y181C, G190A, E203K, Q207E, H208Y, R211A, F214L, T215F, K219Q, K223Q, L228H, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39N+p.M41L+p.E44D+p.V60VI+p.D67N+p.K70KR+p.L74LI+p.V118VI+p.K122E+p.M184V+p.G196GE+p.T200TA+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219Q+p.K223E+p.L228H+p.T286A+p.E297K	Missense mutation	19	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39N, M41L, E44D, V60VI, D67N, K70KR, L74LI, V118VI, K122E, M184V, G196GE, T200TA, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219Q, K223E, L228H, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39N+p.M41L+p.K43E+p.A98G+p.V118I+p.K122P+p.D177E+p.V179I+p.M184MV+p.T200TI+p.Q207E+p.H208HY+p.L210W+p.R211K+p.T215Y+p.L228LHQ+p.Q278H+p.T286TA+p.E291D+p.V292I+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39N, M41L, K43E, A98G, V118I, K122P, D177E, V179I, M184MV, T200TI, Q207E, H208HY, L210W, R211K, T215Y, L228LHQ, Q278H, T286TA, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39N+p.M41L+p.K43Q+p.V60I+p.D67N+p.T69D+p.V75M+p.K101H+p.V118I+p.D121DH+p.K122E+p.I135T+p.I142V+p.S162D+p.K166R+p.E169ED+p.M184V+p.G190A+p.G196E+p.H208FY+p.L210W+p.R211K+p.T215F+p.K219W+p.L228H+p.Q242H+p.V245E+p.A272P+p.L283I+p.A288S+p.E297K	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39N, M41L, K43Q, V60I, D67N, T69D, V75M, K101H, V118I, D121DH, K122E, I135T, I142V, S162D, K166R, E169ED, M184V, G190A, G196E, H208FY, L210W, R211K, T215F, K219W, L228H, Q242H, V245E, A272P, L283I, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.D121Y+p.K122E+p.I135T+p.I142IT+p.Q174QK+p.M184V+p.T200A+p.L210LW+p.R211RK+p.T215Y+p.V245EK+p.A272P	Missense mutation	20	Low-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, D121Y, K122E, I135T, I142IT, Q174QK, M184V, T200A, L210LW, R211RK, T215Y, V245EK, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.D67N+p.T69D+p.K70R+p.L74I+p.V75T+p.K101KQ+p.K122E+p.A129AV+p.I135T+p.I178M+p.V179VI+p.Y181C+p.Q207QE+p.L210LW+p.T215F+p.D218E+p.K219Q+p.L228H	Missense mutation	64	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, D67N, T69D, K70R, L74I, V75T, K101KQ, K122E, A129AV, I135T, I178M, V179VI, Y181C, Q207QE, L210LW, T215F, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.E44D+p.D67N+p.L74LV+p.A98AE+p.K103KN+p.V118I+p.K122E+p.D177DG+p.M184V+p.G196E+p.T200I+p.E203K+p.Q207E+p.H208Y+p.L210W+p.T215Y+p.K219N+p.K223E+p.E224K	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, E44D, D67N, L74LV, A98AE, K103KN, V118I, K122E, D177DG, M184V, G196E, T200I, E203K, Q207E, H208Y, L210W, T215Y, K219N, K223E, E224K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.I94IM+p.K122E+p.I142V+p.V179VI+p.M184V+p.Y188L+p.L210W+p.R211RK+p.T215Y	Missense mutation	21	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, I94IM, K122E, I142V, V179VI, M184V, Y188L, L210W, R211RK, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K43E+p.E44A+p.D67N+p.T69D+p.I94IV+p.A98S+p.V118I+p.K122E+p.D123E+p.I135T+p.I142V+p.D177G+p.I178IM+p.M184V+p.G196E+p.Q197H+p.I202IV+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.L228H	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K43E, E44A, D67N, T69D, I94IV, A98S, V118I, K122E, D123E, I135T, I142V, D177G, I178IM, M184V, G196E, Q197H, I202IV, H208Y, L210W, R211K, T215Y, K219N, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K43E+p.E44D+p.D67N+p.L74I+p.K101H+p.K103R+p.V108I+p.V118I+p.K122P+p.D123E+p.T139R+p.S162C+p.S163SG+p.D177E+p.Y181C+p.M184V+p.G190A+p.I202V+p.H208F+p.L210W+p.R211K+p.T215Y+p.K219KN+p.H221Y+p.A272S+p.K277R+p.A288S+p.I293V+p.E297EQ	Missense mutation	17	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K43E, E44D, D67N, L74I, K101H, K103R, V108I, V118I, K122P, D123E, T139R, S162C, S163SG, D177E, Y181C, M184V, G190A, I202V, H208F, L210W, R211K, T215Y, K219KN, H221Y, A272S, K277R, A288S, I293V, E297EQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K43KN+p.V60I+p.D67N+p.V118I+p.K122E+p.F130FL+p.T131TP+p.E138E*+p.I142V+p.S162A+p.F171Y+p.L210W+p.R211K+p.T215Y+p.K219KN+p.D237DY	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K43KN, V60I, D67N, V118I, K122E, F130FL, T131TP, E138E*, I142V, S162A, F171Y, L210W, R211K, T215Y, K219KN, D237DY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K43Q+p.E44D+p.D67N+p.L74V+p.A98G+p.K101E+p.V118I+p.K122KE+p.S162CY+p.V179I+p.Y181C+p.M184V+p.G190A+p.G196E+p.T200A+p.I202T+p.E203K+p.Q207E+p.H208HY+p.L210W+p.T215Y+p.K219N+p.K223E+p.L228H	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K43Q, E44D, D67N, L74V, A98G, K101E, V118I, K122KE, S162CY, V179I, Y181C, M184V, G190A, G196E, T200A, I202T, E203K, Q207E, H208HY, L210W, T215Y, K219N, K223E, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K43Q+p.E44D+p.K49R+p.V60I+p.D67N+p.K101H+p.V106VI+p.V118I+p.K122E+p.D123E+p.I135T+p.Y181C+p.M184V+p.G190A+p.T200A+p.L210W+p.T215Y+p.D218E+p.K219DN+p.D250E+p.L283I+p.R284K+p.I293V+p.P294Q+p.E297A	Missense mutation	24	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K43Q, E44D, K49R, V60I, D67N, K101H, V106VI, V118I, K122E, D123E, I135T, Y181C, M184V, G190A, T200A, L210W, T215Y, D218E, K219DN, D250E, L283I, R284K, I293V, P294Q, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TK+p.M41L+p.K43KN+p.V60VI+p.R83K+p.K103N+p.V118VIM+p.S134X+p.I135EV+p.V148VE+p.P150PT+p.Q207QE+p.L210LW+p.T215Y+p.I244IK	Missense mutation	57	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TK, M41L, K43KN, V60VI, R83K, K103N, V118VIM, S134X, I135EV, V148VE, P150PT, Q207QE, L210LW, T215Y, I244IK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TS+p.M41L+p.K49KR+p.V60VI+p.S68G+p.A98G+p.K103N+p.K122E+p.I142IL+p.T165L+p.K173Q+p.M184V+p.L210W+p.T215Y	Missense mutation	32	Intermediate resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TS, M41L, K49KR, V60VI, S68G, A98G, K103N, K122E, I142IL, T165L, K173Q, M184V, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69S+p.K70R+p.A98S+p.K103N+p.K122E+p.I135L+p.Q174H+p.D177G+p.V179I+p.Y188L+p.T200A+p.I202V+p.R211K+p.F214L+p.K219Q+p.A272P+p.E297A	Missense mutation	11	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69S, K70R, A98S, K103N, K122E, I135L, Q174H, D177G, V179I, Y188L, T200A, I202V, R211K, F214L, K219Q, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.I31L+p.M41L+p.A62AV+p.V75I+p.V108I+p.V118I+p.V179I+p.Y181C+p.M184V+p.L210W+p.R211K+p.T215Y+p.H221HY+p.L228R+p.Q242QL	Missense mutation	55	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, I31L, M41L, A62AV, V75I, V108I, V118I, V179I, Y181C, M184V, L210W, R211K, T215Y, H221HY, L228R, Q242QL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.D67N+p.T69D+p.K70R+p.K102KR+p.V118I+p.D123E+p.I135T+p.K173KR+p.V179E+p.M184V+p.G196GE+p.Q197K+p.T200A+p.F214FL+p.T215F+p.K219Q+p.L228LH+p.E248ED+p.A272P+p.E297EK	Missense mutation	21	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, D67N, T69D, K70R, K102KR, V118I, D123E, I135T, K173KR, V179E, M184V, G196GE, Q197K, T200A, F214FL, T215F, K219Q, L228LH, E248ED, A272P, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V35M+p.T39A+p.M41L+p.V60I+p.D67N+p.K70R+p.R83K+p.D177E+p.Q197QE+p.T215Y+p.K219E+p.L228H+p.V245VA+p.K277R+p.R284K+p.T286A+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V35M, T39A, M41L, V60I, D67N, K70R, R83K, D177E, Q197QE, T215Y, K219E, L228H, V245VA, K277R, R284K, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V35T+p.M41L+p.K43Q+p.V60I+p.D123E+p.I132IV+p.T139R+p.M184V+p.R211K+p.T215Y+p.Q278QH	Missense mutation	51	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V35T, M41L, K43Q, V60I, D123E, I132IV, T139R, M184V, R211K, T215Y, Q278QH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35A+p.T39A+p.V60I+p.D67N+p.K70R+p.K102E+p.I135V+p.S162Y+p.Q174H+p.R211K+p.T215V+p.K219Q+p.A272P+p.T286A	Missense mutation	30	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35A, T39A, V60I, D67N, K70R, K102E, I135V, S162Y, Q174H, R211K, T215V, K219Q, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.D67G+p.L74I+p.L100I+p.K103R+p.K122E+p.V179D+p.M184V+p.I202V+p.R211K+p.T215Y+p.M230L+p.T240K	Missense mutation	30	Intermediate resistance	[6]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, D67G, L74I, L100I, K103R, K122E, V179D, M184V, I202V, R211K, T215Y, M230L, T240K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.G45E+p.K49R+p.D67N+p.L74LI+p.K103NS+p.V118I+p.K122E+p.D123EK+p.I135T+p.D177DG+p.M184V+p.G196E+p.L210W+p.R211RK+p.T215Y+p.V245VI+p.K249Q+p.S251I+p.E297K	Missense mutation	10	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, G45E, K49R, D67N, L74LI, K103NS, V118I, K122E, D123EK, I135T, D177DG, M184V, G196E, L210W, R211RK, T215Y, V245VI, K249Q, S251I, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.S48T+p.D67N+p.T69D+p.K70R+p.K122E+p.I135T+p.E169D+p.M184V+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.L228H	Missense mutation	18	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, S48T, D67N, T69D, K70R, K122E, I135T, E169D, M184V, H208Y, R211K, T215F, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.E40F+p.M41L+p.K43E+p.D67N+p.K70A+p.L74V+p.A98S+p.V111VIM+p.V118I+p.K122E+p.I135T+p.I142IV+p.G196E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, E40F, M41L, K43E, D67N, K70A, L74V, A98S, V111VIM, V118I, K122E, I135T, I142IV, G196E, H208Y, L210W, R211K, T215Y, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.E44D+p.V60I+p.D67N+p.V118I+p.K122E+p.I135T+p.S162C+p.M184V+p.Y188L+p.G196E+p.T200A+p.E203K+p.L210W+p.R211K+p.T215Y+p.K219DN+p.K223E+p.L228HR+p.A272P+p.A288AS	Missense mutation	17	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, E44D, V60I, D67N, V118I, K122E, I135T, S162C, M184V, Y188L, G196E, T200A, E203K, L210W, R211K, T215Y, K219DN, K223E, L228HR, A272P, A288AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.K43E+p.E44D+p.D67N+p.L74I+p.V106I+p.T107S+p.V108VI+p.V118I+p.K122E+p.I135T+p.I142V+p.V179I+p.M184V+p.Y188L+p.V189I+p.T200K+p.I202V+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221Y+p.V245K+p.K275R+p.T286P+p.E297K	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, K43E, E44D, D67N, L74I, V106I, T107S, V108VI, V118I, K122E, I135T, I142V, V179I, M184V, Y188L, V189I, T200K, I202V, E203D, Q207E, H208Y, L210W, R211K, T215Y, H221Y, V245K, K275R, T286P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39TA+p.K64N+p.D67N+p.T69T_T+p.K70R+p.A98G+p.K102KR+p.K103KN+p.V108I+p.D121C+p.K122E+p.K166R+p.Q174QH+p.D177E+p.G196E+p.E203K+p.Q207E+p.R211K+p.F214FL+p.K219Q+p.H221Y+p.L228N+p.V245T+p.A272P+p.K277R+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39TA, K64N, D67N, T69T_T, K70R, A98G, K102KR, K103KN, V108I, D121C, K122E, K166R, Q174QH, D177E, G196E, E203K, Q207E, R211K, F214FL, K219Q, H221Y, L228N, V245T, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.T39A+p.M41L+p.V60I+p.K70KR+p.K103N+p.D121H+p.K122E+p.D177E+p.I178M+p.M184V+p.G196E+p.E203K+p.L210W+p.T215Y+p.K219E+p.K223E+p.L228H+p.A272P+p.T286A+p.E297R	Missense mutation	32	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, T39A, M41L, V60I, K70KR, K103N, D121H, K122E, D177E, I178M, M184V, G196E, E203K, L210W, T215Y, K219E, K223E, L228H, A272P, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IT+p.D67N+p.K70R+p.K103KN+p.K122E+p.A158S+p.G190GA+p.Q207K+p.R211KT+p.K219KN+p.V245T+p.T286A+p.A288S+p.E297K	Missense mutation	30	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IT, D67N, K70R, K103KN, K122E, A158S, G190GA, Q207K, R211KT, K219KN, V245T, T286A, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IT+p.M41L+p.K43Q+p.A62V+p.K64KR+p.K65R+p.S68K+p.V75I+p.W88S+p.V90VI+p.K101Q+p.Y115F+p.F116Y+p.V118I+p.K122E+p.D123E+p.Q151M+p.R172K+p.K173R+p.Q174R+p.G190S+p.I202V+p.L210W+p.F214L+p.T215E+p.H221HY+p.V245E+p.D250DE+p.A272P+p.I274V+p.K281R+p.T286A+p.A288S+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IT, M41L, K43Q, A62V, K64KR, K65R, S68K, V75I, W88S, V90VI, K101Q, Y115F, F116Y, V118I, K122E, D123E, Q151M, R172K, K173R, Q174R, G190S, I202V, L210W, F214L, T215E, H221HY, V245E, D250DE, A272P, I274V, K281R, T286A, A288S, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35K+p.T39A+p.M41L+p.K43E+p.L74I+p.K101H+p.V108I+p.V118I+p.K122KE+p.D123E+p.I135T+p.Q174QH+p.Y181C+p.M184V+p.G196E+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215F+p.K219W+p.L228K+p.V245K+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35K, T39A, M41L, K43E, L74I, K101H, V108I, V118I, K122KE, D123E, I135T, Q174QH, Y181C, M184V, G196E, Q207E, H208Y, L210W, R211K, T215F, K219W, L228K, V245K, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35K+p.T39A+p.M41L+p.K43E+p.V60I+p.D67N+p.K103N+p.V118I+p.K122E+p.I135T+p.E169D+p.L210W+p.R211K+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35K, T39A, M41L, K43E, V60I, D67N, K103N, V118I, K122E, I135T, E169D, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35K+p.T39A+p.M41L+p.K43KEQ+p.K49KR+p.V60I+p.D67N+p.V90VI+p.K103KN+p.V118I+p.I135V+p.S162D+p.V179VI+p.M184V+p.T200A+p.Q207K+p.L210W+p.R211T+p.T215Y+p.D218DE	Missense mutation	34	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35K, T39A, M41L, K43KEQ, K49KR, V60I, D67N, V90VI, K103KN, V118I, I135V, S162D, V179VI, M184V, T200A, Q207K, L210W, R211T, T215Y, D218DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.A62V+p.D67EK+p.T69S_SS+p.A98S+p.V106I+p.K122E+p.D123DN+p.I135T+p.M184V+p.Y188L+p.V189I+p.T200A+p.T215Y+p.L228H+p.T286A+p.E297R	Missense mutation	42	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, A62V, D67EK, T69S_SS, A98S, V106I, K122E, D123DN, I135T, M184V, Y188L, V189I, T200A, T215Y, L228H, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.M41L+p.K43E+p.V75L+p.R83K+p.V118I+p.D121Y+p.K122E+p.T200A+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	30	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, M41L, K43E, V75L, R83K, V118I, D121Y, K122E, T200A, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.T39A+p.M41L+p.D67N+p.K104R+p.V118VI+p.K122E+p.M184V+p.T200A+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219E	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, T39A, M41L, D67N, K104R, V118VI, K122E, M184V, T200A, Q207E, L210W, R211K, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.D67N+p.K70R+p.W88WS+p.K102KE+p.K103NS+p.V106VI+p.K122E+p.D123DE+p.I135L+p.K166R+p.V189VI+p.G190A+p.G196K+p.T215F+p.D218E+p.K219Q	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, D67N, K70R, W88WS, K102KE, K103NS, V106VI, K122E, D123DE, I135L, K166R, V189VI, G190A, G196K, T215F, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K43Q+p.K49R+p.D67G+p.S68G+p.K70R+p.V111I+p.D121H+p.K122E+p.D123S+p.I135T+p.E169ED+p.Q174R+p.V179I+p.Y181YC+p.M184V+p.G196GE+p.T200A+p.H208HY+p.L210W+p.R211K+p.T215F+p.D218E+p.K219W	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K43Q, K49R, D67G, S68G, K70R, V111I, D121H, K122E, D123S, I135T, E169ED, Q174R, V179I, Y181YC, M184V, G196GE, T200A, H208HY, L210W, R211K, T215F, D218E, K219W.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.M41L+p.S48E+p.D67N+p.T69D+p.K70R+p.L74I+p.I94L+p.K103KN+p.V108I+p.D123E+p.I135V+p.K166KQ+p.D177E+p.M184V+p.G196E+p.T200TA+p.E203D+p.H208Y+p.T215F+p.K219W+p.L228H+p.K238T	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, M41L, S48E, D67N, T69D, K70R, L74I, I94L, K103KN, V108I, D123E, I135V, K166KQ, D177E, M184V, G196E, T200TA, E203D, H208Y, T215F, K219W, L228H, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.T39A+p.M41L+p.K43Q+p.D67DN+p.K122E+p.D123E+p.I135T+p.I142IT+p.E169D+p.V179I+p.T200A+p.L210W+p.R211K+p.T215Y	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, T39A, M41L, K43Q, D67DN, K122E, D123E, I135T, I142IT, E169D, V179I, T200A, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.V60I+p.D67N+p.K70R+p.K103N+p.D123E+p.I135T+p.K166R+p.D177E+p.I178IM+p.V179VI+p.M184V+p.T200A+p.E203D+p.Q207E+p.H208HY+p.R211K+p.F214I+p.T215F+p.K219Q+p.L228H	Missense mutation	71	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, V60I, D67N, K70R, K103N, D123E, I135T, K166R, D177E, I178IM, V179VI, M184V, T200A, E203D, Q207E, H208HY, R211K, F214I, T215F, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39A+p.M41L+p.V60I+p.D67N+p.T69N+p.K70R+p.A98S+p.K101AV+p.V108VI+p.I135T+p.S162SN+p.I178IM+p.V179I+p.M184V+p.G190A+p.Q207E+p.R211K+p.T215FI+p.K219E+p.L228H	Missense mutation	65	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39A, M41L, V60I, D67N, T69N, K70R, A98S, K101AV, V108VI, I135T, S162SN, I178IM, V179I, M184V, G190A, Q207E, R211K, T215FI, K219E, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39TA+p.K49R+p.V60I+p.D67G+p.S68G+p.K70R+p.V90VI+p.K101E+p.V108I+p.I135R+p.V179D+p.Y181C+p.T200A+p.Q207A+p.R211K+p.T215I+p.D218E+p.K219E+p.L228R+p.A272P+p.T286TA+p.E297K	Missense mutation	62	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39TA, K49R, V60I, D67G, S68G, K70R, V90VI, K101E, V108I, I135R, V179D, Y181C, T200A, Q207A, R211K, T215I, D218E, K219E, L228R, A272P, T286TA, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T58N+p.A62V+p.S68G+p.K70N+p.K73L+p.V75I+p.F77L+p.Y115F+p.F116Y+p.K122E+p.I135T+p.T139S+p.Y146F+p.Q151M+p.Q174R+p.Y181C+p.M184V+p.G190A+p.E203K+p.Q207E+p.F214L+p.T215Y+p.K223N+p.L228H+p.Q269QR+p.K277R+p.Q278H+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T58N, A62V, S68G, K70N, K73L, V75I, F77L, Y115F, F116Y, K122E, I135T, T139S, Y146F, Q151M, Q174R, Y181C, M184V, G190A, E203K, Q207E, F214L, T215Y, K223N, L228H, Q269QR, K277R, Q278H, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VE+p.M41L+p.V60VI+p.S68G+p.V90VI+p.K102R+p.K103N+p.S105ST+p.V106A+p.D121H+p.K122E+p.T139TK+p.S162A+p.V179I+p.M184V+p.T200A+p.I202IV+p.Q207R+p.L210W+p.R211K+p.T215SY+p.H221HY+p.F227FL+p.R284RK	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VE, M41L, V60VI, S68G, V90VI, K102R, K103N, S105ST, V106A, D121H, K122E, T139TK, S162A, V179I, M184V, T200A, I202IV, Q207R, L210W, R211K, T215SY, H221HY, F227FL, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.E40FL+p.M41L+p.K43E+p.D67N+p.T69TA+p.L74I+p.K101H+p.V118I+p.K122E+p.S162C+p.E169D+p.M184V+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219D+p.V245M+p.T286A+p.I293V+p.Q334E+p.R358K+p.K366R	Missense mutation	58	Intermediate resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, E40FL, M41L, K43E, D67N, T69TA, L74I, K101H, V118I, K122E, S162C, E169D, M184V, H208Y, L210W, R211K, T215Y, D218E, K219D, V245M, T286A, I293V, Q334E, R358K, K366R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.M41L+p.K43E+p.E44D+p.D67N+p.V118I+p.Q174K+p.I178IL+p.G196E+p.E203K+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223KQ	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, M41L, K43E, E44D, D67N, V118I, Q174K, I178IL, G196E, E203K, Q207D, H208Y, L210W, R211K, T215Y, K219R, K223KQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D67N+p.K70R+p.K103KN+p.V118I+p.D121Y+p.K122E+p.I135T+p.T139TI+p.F171FY+p.M184V+p.T200A+p.Q207E+p.F214L+p.T215V+p.D218E+p.K219Q	Missense mutation	24	Low-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D67N, K70R, K103KN, V118I, D121Y, K122E, I135T, T139TI, F171FY, M184V, T200A, Q207E, F214L, T215V, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8I+p.V21I+p.V35T+p.M41L+p.V60I+p.D67N+p.K101E+p.K122E+p.D123G+p.M184V+p.G190GS+p.H198E+p.I202V+p.L210W+p.T215Y	Missense mutation	13	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8I, V21I, V35T, M41L, V60I, D67N, K101E, K122E, D123G, M184V, G190GS, H198E, I202V, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.T39TA+p.M41L+p.K103N+p.D121DY+p.K122E+p.I135T+p.I142T+p.M184MV+p.T200A+p.L210LW+p.R211K+p.T215SY+p.V245EK+p.A272P	Missense mutation	41	Intermediate resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, T39TA, M41L, K103N, D121DY, K122E, I135T, I142T, M184MV, T200A, L210LW, R211K, T215SY, V245EK, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A33V+p.V35T+p.A62V+p.K65R+p.S68G+p.V75I+p.F77L+p.K122E+p.D123N+p.Q151M+p.I178L+p.T200A+p.E248D+p.A272P+p.D320DE+p.G333E+p.Q334QR+p.T338S+p.A376T+p.T386I+p.E399D+p.A400T+p.V435A+p.F440Y+p.D460N+p.R461K+p.D471N+p.Q480E+p.L491S+p.A508S+p.K512Q+p.S519N+p.K530R+p.A534T+p.K558R	Missense mutation	100	High-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A33V, V35T, A62V, K65R, S68G, V75I, F77L, K122E, D123N, Q151M, I178L, T200A, E248D, A272P, D320DE, G333E, Q334QR, T338S, A376T, T386I, E399D, A400T, V435A, F440Y, D460N, R461K, D471N, Q480E, L491S, A508S, K512Q, S519N, K530R, A534T, K558R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.V75I+p.F77L+p.F116Y+p.K122E+p.Q151M+p.M184V+p.F214L+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, V75I, F77L, F116Y, K122E, Q151M, M184V, F214L, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98AS+p.V118VI+p.K122KE+p.D177E+p.M184V+p.Q207E+p.R211K+p.T215Y+p.M230L+p.D250E+p.A272P+p.I274V	Missense mutation	13	Potential low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98AS, V118VI, K122KE, D177E, M184V, Q207E, R211K, T215Y, M230L, D250E, A272P, I274V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CR+p.M41L+p.L100I+p.K103N+p.I135T+p.T215F+p.D218E+p.L228R+p.K249KR+p.A272P+p.E297A	Missense mutation	50	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CR, M41L, L100I, K103N, I135T, T215F, D218E, L228R, K249KR, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.K70R+p.V75T+p.K103N+p.V118I+p.Y181C+p.L210LW+p.K219Q	Missense mutation	24	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, K70R, V75T, K103N, V118I, Y181C, L210LW, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.A98S+p.K101E+p.K122E+p.D123E+p.D177E+p.I178L+p.V179I+p.G190A+p.T215I+p.K219Q+p.V245E+p.K277R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, A98S, K101E, K122E, D123E, D177E, I178L, V179I, G190A, T215I, K219Q, V245E, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.A98S+p.K122E+p.D123E+p.D177E+p.I178L+p.V179I+p.G190A+p.T215F+p.K219Q+p.V245E+p.K277R+p.T286A+p.E297K+p.K311R	Missense mutation	100	High-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, A98S, K122E, D123E, D177E, I178L, V179I, G190A, T215F, K219Q, V245E, K277R, T286A, E297K, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K103N+p.Y181C+p.T215Y+p.K219Q	Missense mutation	36.4	Intermediate resistance	[10]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K103N, Y181C, T215Y, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K122E+p.D123S+p.I135V+p.K166R+p.D177E+p.T200I+p.E203D+p.R211K+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228LH+p.V245M+p.A272P	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K122E, D123S, I135V, K166R, D177E, T200I, E203D, R211K, F214L, T215F, D218E, K219Q, L228LH, V245M, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K122E+p.Q174R+p.D177E+p.I195M+p.G196E+p.Q207G+p.F214L+p.K219Q+p.A288S	Missense mutation	12	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K122E, Q174R, D177E, I195M, G196E, Q207G, F214L, K219Q, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.L100I+p.K102R+p.K103N+p.I135V+p.I142V+p.Q174H+p.V179I+p.G196E+p.R211G+p.F214L+p.T215F+p.K219Q+p.A272S+p.I293V+p.E297A+p.K311R	Missense mutation	35	Intermediate resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, L100I, K102R, K103N, I135V, I142V, Q174H, V179I, G196E, R211G, F214L, T215F, K219Q, A272S, I293V, E297A, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K70R+p.V106A+p.K122E+p.F214L+p.T215F+p.K219Q+p.F227L+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K70R, V106A, K122E, F214L, T215F, K219Q, F227L, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K70R+p.V90I+p.K103N+p.K122E+p.I135T+p.S162A+p.T215F+p.K219Q+p.L228H+p.V245T+p.D250E	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K70R, V90I, K103N, K122E, I135T, S162A, T215F, K219Q, L228H, V245T, D250E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69ND+p.K70R+p.K103S+p.K219Q	Missense mutation	31	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69ND, K70R, K103S, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67NH+p.K70R+p.K101E+p.Y181C+p.M184V+p.G190A+p.T215F+p.K219Q	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67NH, K70R, K101E, Y181C, M184V, G190A, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28A+p.M41L+p.V60I+p.D67G+p.S68G+p.K70R+p.L74V+p.K101E+p.K122E+p.I135T+p.I178M+p.Y181V+p.G190A+p.T215Y+p.K219E	Missense mutation	100	High-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28A, M41L, V60I, D67G, S68G, K70R, L74V, K101E, K122E, I135T, I178M, Y181V, G190A, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28ED+p.T39A+p.M41L+p.K49R+p.K122E+p.D123N+p.F130FC+p.I135T+p.I142V+p.R143RI+p.N147NT+p.M184V+p.T215Y+p.S251I+p.I257L+p.A272P+p.Q278QH+p.T286TA+p.E297A	Missense mutation	12	Potential low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28ED, T39A, M41L, K49R, K122E, D123N, F130FC, I135T, I142V, R143RI, N147NT, M184V, T215Y, S251I, I257L, A272P, Q278QH, T286TA, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EK+p.K32KE+p.M41L+p.K43KE+p.V60VI+p.D67N+p.L74LI+p.D86E+p.W88WC+p.V90VI+p.K101KE+p.K103N+p.V118VI+p.K122P+p.I135IT+p.K173Q+p.I178L+p.L187LI+p.L210W+p.R211K+p.T215Y+p.A272P+p.V276T+p.P294T	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EK, K32KE, M41L, K43KE, V60VI, D67N, L74LI, D86E, W88WC, V90VI, K101KE, K103N, V118VI, K122P, I135IT, K173Q, I178L, L187LI, L210W, R211K, T215Y, A272P, V276T, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E36D+p.D67DN+p.K70KR+p.K122KE+p.M184MV+p.E203K+p.R211EQ+p.A272P+p.K277KR+p.A288S+p.I293V+p.P294S+p.S322T+p.N348NI+p.M357T+p.K366R	Missense mutation	14	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E36D, D67DN, K70KR, K122KE, M184MV, E203K, R211EQ, A272P, K277KR, A288S, I293V, P294S, S322T, N348NI, M357T, K366R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40F+p.M41L+p.K43E+p.D67N+p.K104R+p.V106I+p.T107P+p.V118I+p.M184V+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R	Missense mutation	59	Intermediate resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40F, M41L, K43E, D67N, K104R, V106I, T107P, V118I, M184V, Q207E, H208Y, L210W, R211K, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40X+p.M41L+p.K43E+p.E44EA+p.K64H+p.D67N+p.T69N+p.K70R+p.L74LI+p.A98AG+p.K122E+p.D123E+p.S162SN+p.Q174K+p.D177E+p.I178M+p.V179VI+p.M184V+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219Q+p.L228H+p.Q242QH+p.A272P+p.I293IV	Missense mutation	100	High-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40X, M41L, K43E, E44EA, K64H, D67N, T69N, K70R, L74LI, A98AG, K122E, D123E, S162SN, Q174K, D177E, I178M, V179VI, M184V, Q207E, H208Y, L210W, R211K, T215Y, K219Q, L228H, Q242QH, A272P, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.K43Q+p.D67DN+p.A98S+p.V118I+p.K122E+p.I135V+p.S162N+p.M184V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219KN+p.A272P+p.L283I+p.I293IV+p.P294T	Missense mutation	23	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, K43Q, D67DN, A98S, V118I, K122E, I135V, S162N, M184V, Q207E, L210W, R211K, T215Y, K219KN, A272P, L283I, I293IV, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T39TA+p.M41L+p.V60I+p.D67N+p.K70R+p.K103R+p.V118I+p.K122E+p.F124FL+p.T131TRS+p.I142IM+p.Q197K+p.T200E+p.E203D+p.Q207D+p.F214L+p.T215F+p.K219Q+p.K223Q+p.L228LH+p.Q242K+p.V245M+p.A272P+p.V276T+p.L283I+p.A288T+p.I293V+p.E297A	Missense mutation	83	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T39TA, M41L, V60I, D67N, K70R, K103R, V118I, K122E, F124FL, T131TRS, I142IM, Q197K, T200E, E203D, Q207D, F214L, T215F, K219Q, K223Q, L228LH, Q242K, V245M, A272P, V276T, L283I, A288T, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.K20R+p.M41L+p.K43Q+p.V60I+p.D67N+p.A98AG+p.K103KN+p.V118I+p.I135L+p.M184V+p.T200TA+p.E203D+p.L210W+p.R211K+p.T215Y+p.V245Q+p.A272P+p.I293V+p.E297K+p.Q334C	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, K20R, M41L, K43Q, V60I, D67N, A98AG, K103KN, V118I, I135L, M184V, T200TA, E203D, L210W, R211K, T215Y, V245Q, A272P, I293V, E297K, Q334C.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.T39AE+p.M41L+p.D67DN+p.K70KR+p.I94IL+p.A98G+p.K102KQ+p.K122E+p.D123E+p.I135L+p.I142V+p.S162A+p.D177E+p.V179I+p.M184V+p.G190A+p.T215F+p.K219KE+p.V245T+p.A272P+p.I293V+p.E297A	Missense mutation	100	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, T39AE, M41L, D67DN, K70KR, I94IL, A98G, K102KQ, K122E, D123E, I135L, I142V, S162A, D177E, V179I, M184V, G190A, T215F, K219KE, V245T, A272P, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.M41L+p.K43E+p.E44A+p.D67N+p.T69D+p.V75M+p.W88C+p.I94L+p.A98G+p.V108I+p.V118I+p.K122E+p.I135V+p.I142IV+p.D177E+p.I178M+p.V179E+p.M184V+p.V189I+p.G196E+p.T200A+p.E203D+p.H208Y+p.L210W+p.R211D+p.T215F+p.H221Y+p.A272P+p.A288S	Missense mutation	100	High-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, M41L, K43E, E44A, D67N, T69D, V75M, W88C, I94L, A98G, V108I, V118I, K122E, I135V, I142IV, D177E, I178M, V179E, M184V, V189I, G196E, T200A, E203D, H208Y, L210W, R211D, T215F, H221Y, A272P, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.V35M+p.T39A+p.M41L+p.K43E+p.T69D+p.V75M+p.A98G+p.K101E+p.V118I+p.K122E+p.P170PL+p.Y181C+p.G190A+p.E203K+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.L228H+p.A272P+p.I293V+p.I329L	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, V35M, T39A, M41L, K43E, T69D, V75M, A98G, K101E, V118I, K122E, P170PL, Y181C, G190A, E203K, Q207D, H208Y, L210W, R211K, T215Y, K219R, L228H, A272P, I293V, I329L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I2V+p.V35L+p.T39A+p.M41L+p.E44D+p.D67N+p.K70R+p.L74V+p.L100I+p.K102Q+p.K103N+p.V118I+p.K122E+p.A158S+p.S162X+p.E169ED+p.V179VI+p.M184V+p.T200A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219E+p.L228H+p.V245E+p.E297R+p.L301M	Missense mutation	11	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I2V, V35L, T39A, M41L, E44D, D67N, K70R, L74V, L100I, K102Q, K103N, V118I, K122E, A158S, S162X, E169ED, V179VI, M184V, T200A, H208Y, L210W, R211K, T215Y, D218E, K219E, L228H, V245E, E297R, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5IM+p.V35M+p.E40ED+p.M41L+p.D67N+p.K82KN+p.K103N+p.I135T+p.S162A+p.M184V+p.Q207E+p.L210W+p.T215Y+p.K238T+p.A272P+p.S322T	Missense mutation	41	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5IM, V35M, E40ED, M41L, D67N, K82KN, K103N, I135T, S162A, M184V, Q207E, L210W, T215Y, K238T, A272P, S322T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5S+p.K20R+p.E28K+p.K32E+p.V35R+p.M41L+p.E44D+p.D67N+p.K103N+p.D123E+p.I135M+p.K166R+p.D177E+p.M184V+p.G196E+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E+p.E297K	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5S, K20R, E28K, K32E, V35R, M41L, E44D, D67N, K103N, D123E, I135M, K166R, D177E, M184V, G196E, T200A, L210W, R211K, T215Y, V245E, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.D123E+p.I135V+p.Q197E+p.Q207A+p.R211K+p.T215Y+p.V245KN+p.L283I+p.A288AP+p.I293V+p.P294A+p.E297K	Missense mutation	11.2	Potential low-level resistance	[12]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, D123E, I135V, Q197E, Q207A, R211K, T215Y, V245KN, L283I, A288AP, I293V, P294A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.E40ED+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.A98S+p.K122E+p.I135T+p.T215F+p.D218E+p.K219Q+p.L228H	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, E40ED, M41L, V60I, D67N, T69D, K70R, A98S, K122E, I135T, T215F, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35L+p.T39A+p.M41L+p.K43E+p.E44D+p.K49R+p.D67N+p.R83K+p.V118I+p.K122P+p.S162D+p.E169D+p.D177E+p.Y181C+p.T200I+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R+p.K281R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35L, T39A, M41L, K43E, E44D, K49R, D67N, R83K, V118I, K122P, S162D, E169D, D177E, Y181C, T200I, L210W, R211K, T215Y, D218E, K219R, K281R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35L+p.T39TA+p.M41L+p.K43E+p.E44D+p.K49R+p.D67N+p.R83K+p.V118VI+p.K122P+p.S162SN+p.D177E+p.M184V+p.G196GE+p.T200I+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219KR+p.K281R+p.R284RK+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35L, T39TA, M41L, K43E, E44D, K49R, D67N, R83K, V118VI, K122P, S162SN, D177E, M184V, G196GE, T200I, L210W, R211K, T215Y, D218E, K219KR, K281R, R284RK, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.D67G+p.S68G+p.K70R+p.I135V+p.M184V+p.F214L+p.T215F+p.D218DE+p.K219Q	Missense mutation	10	Potential low-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, D67G, S68G, K70R, I135V, M184V, F214L, T215F, D218DE, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E28K+p.K32E+p.V35R+p.M41L+p.E44D+p.D67N+p.K103N+p.D123E+p.I135M+p.P157A+p.K166R+p.D177E+p.M184V+p.G196E+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E+p.E297K	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E28K, K32E, V35R, M41L, E44D, D67N, K103N, D123E, I135M, P157A, K166R, D177E, M184V, G196E, T200A, L210W, R211K, T215Y, V245E, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.A98S+p.K122E+p.A158S+p.M184V+p.Q197E+p.T215Y+p.L228LH+p.V245EK	Missense mutation	10.4	Potential low-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, A98S, K122E, A158S, M184V, Q197E, T215Y, L228LH, V245EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.D67N+p.T69DN+p.K70KR+p.V75I+p.F77L+p.F116Y+p.Q151M+p.S162D+p.K166R+p.M184V+p.G196E+p.I202V+p.Q207E+p.R211RK+p.T215FV+p.K219Q+p.A272P+p.K277KR+p.A288AS+p.E297EKQ	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, D67N, T69DN, K70KR, V75I, F77L, F116Y, Q151M, S162D, K166R, M184V, G196E, I202V, Q207E, R211RK, T215FV, K219Q, A272P, K277KR, A288AS, E297EKQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.E44D+p.V60I+p.D67N+p.T69D+p.K102KE+p.V118VI+p.D121H+p.K122E+p.I135T+p.S162YD+p.K166KR+p.D177E+p.M184V+p.G196GE+p.E203EK+p.Q207E+p.L210W+p.T215Y+p.A272P+p.T286A+p.E297K	Missense mutation	26	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, E44D, V60I, D67N, T69D, K102KE, V118VI, D121H, K122E, I135T, S162YD, K166KR, D177E, M184V, G196GE, E203EK, Q207E, L210W, T215Y, A272P, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43E+p.K49R+p.D67N+p.T69D+p.V75T+p.K101X+p.K102KR+p.V108I+p.V118I+p.K122E+p.K173R+p.Y181C+p.V189I+p.G190S+p.I202T+p.H208Y+p.L210W+p.R211K+p.T215Y+p.D218DE+p.K219N+p.L228H+p.A272AP+p.V292I+p.I293V+p.P294PT+p.E297EA	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43E, K49R, D67N, T69D, V75T, K101X, K102KR, V108I, V118I, K122E, K173R, Y181C, V189I, G190S, I202T, H208Y, L210W, R211K, T215Y, D218DE, K219N, L228H, A272AP, V292I, I293V, P294PT, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43KQ+p.D67N+p.T69N+p.K70R+p.K104N+p.K122E+p.I135T+p.K166R+p.M184V+p.I202V+p.R211K+p.T215F+p.K219Q+p.A272P+p.K277R+p.E302D	Missense mutation	59	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43KQ, D67N, T69N, K70R, K104N, K122E, I135T, K166R, M184V, I202V, R211K, T215F, K219Q, A272P, K277R, E302D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43Q+p.E44D+p.V60I+p.D67N+p.T69D+p.V90I+p.V118I+p.K122E+p.G196E+p.Q197QK+p.I202IV+p.L210W+p.R211K+p.T215Y+p.K219KR+p.S251I+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43Q, E44D, V60I, D67N, T69D, V90I, V118I, K122E, G196E, Q197QK, I202IV, L210W, R211K, T215Y, K219KR, S251I, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43Q+p.V60I+p.A62AG+p.D67N+p.K82KN+p.K103KN+p.V118I+p.I135L+p.K173KR+p.V179VI+p.M184V+p.Y188X+p.E203D+p.L210W+p.R211K+p.T215Y+p.V245EQ+p.A272P+p.I293V+p.E297K+p.E298ED	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43Q, V60I, A62AG, D67N, K82KN, K103KN, V118I, I135L, K173KR, V179VI, M184V, Y188X, E203D, L210W, R211K, T215Y, V245EQ, A272P, I293V, E297K, E298ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43X+p.E44D+p.D67N+p.L74I+p.V118I+p.I135T+p.S162X+p.K173E+p.Q174K+p.D177E+p.M184V+p.G196GE+p.Q197E+p.K201KR+p.I202V+p.E203EK+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221C+p.V245K+p.A272P+p.K277KR+p.R284K+p.T286A+p.I293V+p.E297K	Missense mutation	86	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43X, E44D, D67N, L74I, V118I, I135T, S162X, K173E, Q174K, D177E, M184V, G196GE, Q197E, K201KR, I202V, E203EK, Q207E, H208Y, L210W, R211K, T215Y, H221C, V245K, A272P, K277KR, R284K, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41ML+p.K49R+p.T58TN+p.D67NS+p.T69N+p.K70R+p.V75I+p.F77L+p.K101Q+p.F116Y+p.Q151M+p.S162D+p.K166R+p.K173KE+p.Y181C+p.M184V+p.G190GE+p.G196E+p.I202V+p.Q207E+p.R211RK+p.T215V+p.K219Q+p.A272P+p.A288S+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41ML, K49R, T58TN, D67NS, T69N, K70R, V75I, F77L, K101Q, F116Y, Q151M, S162D, K166R, K173KE, Y181C, M184V, G190GE, G196E, I202V, Q207E, R211RK, T215V, K219Q, A272P, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.R83K+p.V118VI+p.I135V+p.G196E+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R+p.I293IV+p.E297K	Missense mutation	40	Intermediate resistance	[12]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, R83K, V118VI, I135V, G196E, E203K, Q207E, L210W, R211K, T215Y, A272P, K277R, I293IV, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.L74V+p.K122E+p.I135T+p.E138A+p.S162C+p.V179VI+p.Y181I+p.E203K+p.L210W+p.R211K+p.T215Y+p.H221HY+p.D250N	Missense mutation	19	Low-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, L74V, K122E, I135T, E138A, S162C, V179VI, Y181I, E203K, L210W, R211K, T215Y, H221HY, D250N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39TA+p.M41L+p.K43E+p.E44D+p.S48X+p.T69D+p.V118I+p.K122E+p.D123N+p.F130FC+p.I142IV+p.M184V+p.T200A+p.E203K+p.H208Y+p.L210W+p.R211KE+p.F214FL+p.T215Y+p.K219R+p.K223E+p.F227FL+p.L228R+p.M230K+p.D250E+p.Q278H+p.T286A	Missense mutation	27	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39TA, M41L, K43E, E44D, S48X, T69D, V118I, K122E, D123N, F130FC, I142IV, M184V, T200A, E203K, H208Y, L210W, R211KE, F214FL, T215Y, K219R, K223E, F227FL, L228R, M230K, D250E, Q278H, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.E36EK+p.M41L+p.K43N+p.D67N+p.I135V+p.G190E+p.T200A+p.L210W+p.T215Y+p.K219N+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, E36EK, M41L, K43N, D67N, I135V, G190E, T200A, L210W, T215Y, K219N, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.M41L+p.K43Q+p.V60I+p.D67N+p.K103N+p.V108I+p.K122E+p.I135T+p.M184V+p.V189VI+p.G196E+p.L210W+p.T215Y+p.P225H	Missense mutation	47	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, M41L, K43Q, V60I, D67N, K103N, V108I, K122E, I135T, M184V, V189VI, G196E, L210W, T215Y, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.D67N+p.T69N+p.K70R+p.K122E+p.D123N+p.I135V+p.I178IM+p.I195L+p.G196E+p.T200TA+p.E203G+p.Q207EG+p.T215F+p.K219Q+p.V245K+p.E248ED+p.D250DE+p.A272P+p.K277R+p.T286A+p.G333E+p.Q367L	Missense mutation	80	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, D67N, T69N, K70R, K122E, D123N, I135V, I178IM, I195L, G196E, T200TA, E203G, Q207EG, T215F, K219Q, V245K, E248ED, D250DE, A272P, K277R, T286A, G333E, Q367L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22N+p.Q23N+p.M41L+p.S48T+p.D67DN+p.K70R+p.K122P+p.M184V+p.E204ED+p.R211K+p.T215Y+p.A272P	Missense mutation	13	Potential low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22N, Q23N, M41L, S48T, D67DN, K70R, K122P, M184V, E204ED, R211K, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32E+p.V35I+p.K49R+p.A62V+p.V75I+p.F77L+p.F116Y+p.I135V+p.Q151M+p.S162C+p.E169D+p.Q207E+p.R211K+p.F214L+p.A272P+p.E291G+p.I293V	Missense mutation	55	Intermediate resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32E, V35I, K49R, A62V, V75I, F77L, F116Y, I135V, Q151M, S162C, E169D, Q207E, R211K, F214L, A272P, E291G, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32T+p.T39A+p.M41L+p.E44D+p.I47V+p.D67N+p.A98S+p.K103N+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.V245T+p.K277R+p.R284K+p.T286A	Missense mutation	100	High-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32T, T39A, M41L, E44D, I47V, D67N, A98S, K103N, H208Y, L210W, R211K, T215Y, K219N, V245T, K277R, R284K, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43Q+p.K64KN+p.D67N+p.T69N+p.K70R+p.S162A+p.I178M+p.T200A+p.E203K+p.Q207E+p.K219Q+p.K277R+p.I293V	Missense mutation	47	Intermediate resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43Q, K64KN, D67N, T69N, K70R, S162A, I178M, T200A, E203K, Q207E, K219Q, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43Q+p.S48T+p.T69E_KS+p.V75M+p.K82R+p.A98G+p.L100I+p.K103N+p.V118I+p.K122E+p.D123E+p.K166R+p.G196E+p.L210W+p.R211K+p.T215F	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43Q, S48T, T69E_KS, V75M, K82R, A98G, L100I, K103N, V118I, K122E, D123E, K166R, G196E, L210W, R211K, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.D67N+p.K70R+p.I135T+p.V179I+p.M184V+p.Q197K+p.T200A+p.Q207D+p.R211K+p.T215Y+p.K219E+p.V245K+p.K277R+p.I293V	Missense mutation	16	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, D67N, K70R, I135T, V179I, M184V, Q197K, T200A, Q207D, R211K, T215Y, K219E, V245K, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41I+p.D67N+p.K103N+p.M184V+p.L210W+p.T215Y+p.K219N	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41I, D67N, K103N, M184V, L210W, T215Y, K219N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.T69D+p.V75T+p.K101E+p.D123E+p.I135T+p.I142V+p.Y181C+p.G190A+p.L210W+p.R211K+p.T215Y+p.L228R+p.A272S+p.K277R+p.K281R+p.I293V+p.E297K+p.Q334E	Missense mutation	86	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, T69D, V75T, K101E, D123E, I135T, I142V, Y181C, G190A, L210W, R211K, T215Y, L228R, A272S, K277R, K281R, I293V, E297K, Q334E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.T69S_SA+p.K102Q+p.S162C+p.T215Y+p.K277R	Missense mutation	100	High-level resistance	[16]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, T69S_SA, K102Q, S162C, T215Y, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.T69S_SA+p.L74V+p.K122E+p.M184V+p.L210W+p.F214L+p.T215Y+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, T69S_SA, L74V, K122E, M184V, L210W, F214L, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D123E+p.I135T+p.I142T+p.D177E+p.V179I+p.M184V+p.G196E+p.L210W+p.T215Y+p.L228R	Missense mutation	14.1	Potential low-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D123E, I135T, I142T, D177E, V179I, M184V, G196E, L210W, T215Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K103KN+p.M184V+p.L210W+p.T215Y+p.K219Q+p.P236PL	Missense mutation	37.1	Intermediate resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K103KN, M184V, L210W, T215Y, K219Q, P236PL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K122E+p.I135V+p.E138EK+p.I142X+p.S162SC+p.V179ED+p.R211S+p.T215Y+p.S251H+p.T286A	Missense mutation	64	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K122E, I135V, E138EK, I142X, S162SC, V179ED, R211S, T215Y, S251H, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.K103N+p.K122E+p.M184V+p.F214L+p.T215Y+p.K219E+p.A272P+p.K277R	Missense mutation	33.8	Intermediate resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, K103N, K122E, M184V, F214L, T215Y, K219E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.M184V+p.L210W+p.T215Y	Missense mutation	39.8	Intermediate resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, M184V, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.V118I+p.K122E+p.I135T+p.S162D+p.D177E+p.M184V+p.T200E+p.E203D+p.F214L+p.T215F+p.K219Q+p.V245K+p.E297A	Missense mutation	23	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, V118I, K122E, I135T, S162D, D177E, M184V, T200E, E203D, F214L, T215F, K219Q, V245K, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.M184V+p.L210W+p.T215Y	Missense mutation	21.4	Low-level resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, M184V, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.R83K+p.K103N+p.M184V+p.Q207H+p.L210W+p.R211K+p.T215Y+p.A272P+p.K311R	Missense mutation	30	Intermediate resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, R83K, K103N, M184V, Q207H, L210W, R211K, T215Y, A272P, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.T69D+p.K103N+p.L210W+p.T215Y	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, T69D, K103N, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.V118I+p.K122E+p.L210W+p.F214L+p.T215Y+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, V118I, K122E, L210W, F214L, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.V75M+p.K122E+p.L210W+p.F214L+p.T215Y+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[8]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, V75M, K122E, L210W, F214L, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122E+p.I135IT+p.M184V+p.T200A+p.E204K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	21	Low-level resistance	[18]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122E, I135IT, M184V, T200A, E204K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.V60I+p.D67N+p.V75M+p.R83RK+p.I94IL+p.A98G+p.V118I+p.K122E+p.I135V+p.I178L+p.M184V+p.G190Q+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E+p.A272P+p.K277R+p.I293V+p.E297R	Missense mutation	12	Potential low-level resistance	[19]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, V60I, D67N, V75M, R83RK, I94IL, A98G, V118I, K122E, I135V, I178L, M184V, G190Q, T200A, L210W, R211K, T215Y, V245E, A272P, K277R, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KE+p.A62V+p.K64R+p.T69G+p.L74V+p.L100I+p.K103N+p.V111VI+p.K122E+p.I135T+p.S162Y+p.M164L+p.M184V+p.H208L+p.L210W+p.R211K+p.T215Y+p.H221HY+p.L228H+p.V245E+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KE, A62V, K64R, T69G, L74V, L100I, K103N, V111VI, K122E, I135T, S162Y, M164L, M184V, H208L, L210W, R211K, T215Y, H221HY, L228H, V245E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.E44D+p.D67N+p.T69D+p.A98S+p.K103N+p.V118I+p.K122E+p.I135V+p.E138Q+p.S162C+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.V245A+p.Q278H+p.T286A+p.P294S	Missense mutation	100	High-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, E44D, D67N, T69D, A98S, K103N, V118I, K122E, I135V, E138Q, S162C, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, V245A, Q278H, T286A, P294S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.K49KR+p.K103N+p.V118I+p.I135V+p.S162C+p.E203K+p.R206R*+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K223Q+p.L228R+p.Q269QH+p.I293V+p.I326V	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, K49KR, K103N, V118I, I135V, S162C, E203K, R206R*, Q207E, L210W, R211K, T215Y, K223Q, L228R, Q269QH, I293V, I326V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K49R+p.T69S_SS+p.L74V+p.R83K+p.A98G+p.M184V+p.G190C+p.Q197E+p.L210W+p.R211K+p.T215Y+p.L228H+p.K281R+p.I293V+p.E297A	Missense mutation	100	High-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K49R, T69S_SS, L74V, R83K, A98G, M184V, G190C, Q197E, L210W, R211K, T215Y, L228H, K281R, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L210W+p.T215F	Missense mutation	22.1	Low-level resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L210W, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S48T+p.D67N+p.T69N+p.K70R+p.K122E+p.M184V+p.G196E+p.H208Y+p.T215F+p.D218E+p.K219Q+p.V245M+p.A272P+p.A288T+p.E297K	Missense mutation	33	Intermediate resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S48T, D67N, T69N, K70R, K122E, M184V, G196E, H208Y, T215F, D218E, K219Q, V245M, A272P, A288T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.K70R+p.V75VIM+p.L100LI+p.K101KE+p.K103KN+p.D123E+p.G190GRS+p.G196E+p.Q207H+p.T215F+p.K219E+p.A272P+p.Q278H+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, K70R, V75VIM, L100LI, K101KE, K103KN, D123E, G190GRS, G196E, Q207H, T215F, K219E, A272P, Q278H, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.T69N+p.K70R+p.K101H+p.D123E+p.I142V+p.K166R+p.D177E+p.M184V+p.G196E+p.T200I+p.I202V+p.R211K+p.T215F+p.K219Q+p.A272P+p.K277R+p.T286A+p.I293V+p.E297Q+p.I329V	Missense mutation	16	Low-level resistance	[11]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, T69N, K70R, K101H, D123E, I142V, K166R, D177E, M184V, G196E, T200I, I202V, R211K, T215F, K219Q, A272P, K277R, T286A, I293V, E297Q, I329V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.I135V+p.S162C+p.G196E+p.T215Y+p.T286A+p.I293V+p.E297K+p.S322A+p.D324E+p.F346Y+p.M357T+p.R358K+p.G359S+p.K366R+p.A400T	Missense mutation	12.3	Potential low-level resistance	[20]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, I135V, S162C, G196E, T215Y, T286A, I293V, E297K, S322A, D324E, F346Y, M357T, R358K, G359S, K366R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.V118I+p.D121Y+p.K122E+p.I135M+p.M184V+p.L210W+p.T215Y+p.V245R+p.A272P	Missense mutation	29	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, V118I, D121Y, K122E, I135M, M184V, L210W, T215Y, V245R, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60VI+p.D67N+p.A98S+p.K103N+p.K122E+p.T139TI+p.I142IV+p.L210W+p.R211RK+p.T215Y+p.K219R+p.K223X+p.E224EV+p.P226PS+p.K238KI	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60VI, D67N, A98S, K103N, K122E, T139TI, I142IV, L210W, R211RK, T215Y, K219R, K223X, E224EV, P226PS, K238KI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60VI+p.R83K+p.V90VI+p.K122E+p.I135IT+p.S162Y+p.E169ED+p.T200TI+p.E203ED+p.Q207X+p.R211RK+p.T215Y+p.K219KQ+p.A272P	Missense mutation	29	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60VI, R83K, V90VI, K122E, I135IT, S162Y, E169ED, T200TI, E203ED, Q207X, R211RK, T215Y, K219KQ, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K20R+p.T58N+p.A62V+p.D67N+p.S68N+p.K70G+p.V75I+p.F77L+p.K101KQ+p.K103N+p.Y115F+p.F116Y+p.K122E+p.I135T+p.Q151M+p.I167IV+p.V179I+p.M184I+p.Y188H+p.I202V+p.L210LS+p.R211K+p.T215Y+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K20R, T58N, A62V, D67N, S68N, K70G, V75I, F77L, K101KQ, K103N, Y115F, F116Y, K122E, I135T, Q151M, I167IV, V179I, M184I, Y188H, I202V, L210LS, R211K, T215Y, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K20R+p.T58TN+p.A62V+p.D67N+p.S68N+p.K70G+p.V75I+p.F77L+p.K101Q+p.K103N+p.Y115F+p.F116Y+p.K122E+p.I135T+p.Q151M+p.S163ST+p.V179I+p.M184I+p.Y188YH+p.I202V+p.R211K+p.T215Y+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K20R, T58TN, A62V, D67N, S68N, K70G, V75I, F77L, K101Q, K103N, Y115F, F116Y, K122E, I135T, Q151M, S163ST, V179I, M184I, Y188YH, I202V, R211K, T215Y, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.M41L+p.A62V+p.A98G+p.K122E+p.D123N+p.K166R+p.M184V+p.G196E+p.I202V+p.L210W+p.R211K+p.T215Y+p.L228R+p.D237N+p.V245E+p.A272P+p.E297K	Missense mutation	27	Low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, M41L, A62V, A98G, K122E, D123N, K166R, M184V, G196E, I202V, L210W, R211K, T215Y, L228R, D237N, V245E, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4T+p.M41L+p.K43KQ+p.T69S+p.V75M+p.K103N+p.P119I+p.K122E+p.I135T+p.I142T+p.S162H+p.M184V+p.L210W+p.R211K+p.T215Y+p.L228H+p.Q242L+p.V245A+p.A272P	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4T, M41L, K43KQ, T69S, V75M, K103N, P119I, K122E, I135T, I142T, S162H, M184V, L210W, R211K, T215Y, L228H, Q242L, V245A, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K103N+p.V108I+p.I135V+p.E169D+p.Y181C+p.T200A+p.Q207E+p.T215Y+p.H221Y+p.V245T	Missense mutation	28	Low-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K103N, V108I, I135V, E169D, Y181C, T200A, Q207E, T215Y, H221Y, V245T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.K20R+p.M41L+p.K43N+p.D67N+p.K103KN+p.V111VI+p.D123E+p.I135IM+p.I142IV+p.S163T+p.T165I+p.M184V+p.D192DN+p.G196GE+p.E203D+p.Q207E+p.L210LS+p.R211K+p.T215Y+p.H221HY+p.K238KT+p.A272P+p.E297K	Missense mutation	11	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, K20R, M41L, K43N, D67N, K103KN, V111VI, D123E, I135IM, I142IV, S163T, T165I, M184V, D192DN, G196GE, E203D, Q207E, L210LS, R211K, T215Y, H221HY, K238KT, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27A+p.V35Q+p.T39A+p.D67N+p.S68G+p.K70R+p.D123E+p.I135T+p.I142V+p.K166R+p.D177E+p.V179I+p.Y181C+p.G196E+p.E203D+p.T215F+p.K219E+p.A272P+p.K277R+p.L283I+p.A288T+p.E297K	Missense mutation	100	High-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27A, V35Q, T39A, D67N, S68G, K70R, D123E, I135T, I142V, K166R, D177E, V179I, Y181C, G196E, E203D, T215F, K219E, A272P, K277R, L283I, A288T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.V60I+p.D67N+p.T69N+p.K70R+p.K103S+p.I135T+p.Y188H+p.G190A+p.T215F+p.D218E+p.K219E+p.E248Q+p.K249Q+p.A288T+p.P294L	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, V60I, D67N, T69N, K70R, K103S, I135T, Y188H, G190A, T215F, D218E, K219E, E248Q, K249Q, A288T, P294L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27TS+p.K49R+p.D67N+p.K70R+p.A98AG+p.K103KN+p.V108VI+p.K122E+p.I135T+p.M184MV+p.V189I+p.G190GRS+p.I195IV+p.G196GR+p.R211Q+p.F214L+p.T215TI+p.K219Q+p.L228LR+p.Q242QL+p.D250E+p.A272P+p.K311R+p.E312Q+p.S322T+p.I329L	Missense mutation	13	Potential low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27TS, K49R, D67N, K70R, A98AG, K103KN, V108VI, K122E, I135T, M184MV, V189I, G190GRS, I195IV, G196GR, R211Q, F214L, T215TI, K219Q, L228LR, Q242QL, D250E, A272P, K311R, E312Q, S322T, I329L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44D+p.D67N+p.K70R+p.K122E+p.I142V+p.M184V+p.I202V+p.L210W+p.R211K+p.T215Y+p.K219Q+p.A288T	Missense mutation	100	High-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44D, D67N, K70R, K122E, I142V, M184V, I202V, L210W, R211K, T215Y, K219Q, A288T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.E44ED+p.S68G+p.A98G+p.D123E+p.I142IV+p.Q174H+p.M184V+p.G196E+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.A272P+p.K281R+p.T286P+p.E297RK	Missense mutation	33	Intermediate resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, E44ED, S68G, A98G, D123E, I142IV, Q174H, M184V, G196E, E203K, Q207E, L210W, R211K, T215Y, A272P, K281R, T286P, E297RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43A+p.E44D+p.D67N+p.T69D+p.V118I+p.K122E+p.I142V+p.M184V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.I293V+p.E297A	Missense mutation	35.4	Intermediate resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43A, E44D, D67N, T69D, V118I, K122E, I142V, M184V, Q207E, L210W, R211K, T215Y, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.T69N+p.K70R+p.V75M+p.K102KR+p.K103N+p.V106A+p.V118I+p.K122E+p.I135V+p.I142V+p.D177N+p.I178M+p.G196E+p.E203EA+p.H208Y+p.L210W+p.T215Y+p.D218E+p.K219HQ+p.F227L+p.V245K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43E, E44A, V60I, D67N, T69N, K70R, V75M, K102KR, K103N, V106A, V118I, K122E, I135V, I142V, D177N, I178M, G196E, E203EA, H208Y, L210W, T215Y, D218E, K219HQ, F227L, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43E+p.E44A+p.V60I+p.D67N+p.T69N+p.K70R+p.V75M+p.K102KR+p.K103N+p.V106A+p.V118I+p.K122E+p.I135V+p.I142V+p.D177N+p.I178M+p.G196E+p.E203X+p.H208Y+p.L210W+p.T215FY+p.D218E+p.K219HQ+p.F227L+p.V245K	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43E, E44A, V60I, D67N, T69N, K70R, V75M, K102KR, K103N, V106A, V118I, K122E, I135V, I142V, D177N, I178M, G196E, E203X, H208Y, L210W, T215FY, D218E, K219HQ, F227L, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43KN+p.E44ED+p.D67N+p.K70R+p.D123E+p.E169D+p.D177E+p.V179VI+p.M184V+p.T200A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219E+p.L228H+p.V245K+p.A272P+p.A288S+p.E297A	Missense mutation	11	Potential low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43KN, E44ED, D67N, K70R, D123E, E169D, D177E, V179VI, M184V, T200A, H208Y, L210W, R211K, T215Y, K219E, L228H, V245K, A272P, A288S, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43KQ+p.E44D+p.D67N+p.T69D+p.R83RK+p.V118VI+p.K122E+p.I135T+p.E169D+p.D177DG+p.M184V+p.T200E+p.E203K+p.Q207QE+p.H208Y+p.L210W+p.R211K+p.T215Y+p.A272P+p.V276T+p.K277KR	Missense mutation	43	Intermediate resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43KQ, E44D, D67N, T69D, R83RK, V118VI, K122E, I135T, E169D, D177DG, M184V, T200E, E203K, Q207QE, H208Y, L210W, R211K, T215Y, A272P, V276T, K277KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43X+p.D67N+p.L74V+p.K103N+p.V118I+p.D123E+p.I135T+p.D177E+p.I178L+p.M184V+p.G196E+p.Q207K+p.L210W+p.R211D+p.T215Y+p.K238T+p.V245VE+p.A272P+p.T286TA+p.I293IV+p.P294T+p.E297Q	Missense mutation	33	Intermediate resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43X, D67N, L74V, K103N, V118I, D123E, I135T, D177E, I178L, M184V, G196E, Q207K, L210W, R211D, T215Y, K238T, V245VE, A272P, T286TA, I293IV, P294T, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K64R+p.D67N+p.L74I+p.V118I+p.K122E+p.D123DN+p.S162D+p.V179I+p.M184V+p.H208HY+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219Q+p.L228H+p.E291ED+p.T296TS	Missense mutation	15	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K64R, D67N, L74I, V118I, K122E, D123DN, S162D, V179I, M184V, H208HY, L210W, R211K, T215Y, D218E, K219Q, L228H, E291ED, T296TS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TP+p.D67N+p.K70R+p.V90I+p.K101P+p.K102R+p.K103N+p.K122E+p.I135V+p.Q151M+p.G196E+p.Q207K+p.R211A+p.K219Q+p.L228LH+p.E248D+p.I293V+p.E297A	Missense mutation	18	Low-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TP, D67N, K70R, V90I, K101P, K102R, K103N, K122E, I135V, Q151M, G196E, Q207K, R211A, K219Q, L228LH, E248D, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T7TP+p.K20R+p.M41L+p.E44D+p.V60I+p.D67N+p.V75M+p.D123E+p.I142V+p.S162Y+p.M184V+p.G196E+p.Q197K+p.E203D+p.H208F+p.L210W+p.R211K+p.T215Y+p.K219N+p.S251I+p.A272P+p.I293V	Missense mutation	84.2	High-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T7TP, K20R, M41L, E44D, V60I, D67N, V75M, D123E, I142V, S162Y, M184V, G196E, Q197K, E203D, H208F, L210W, R211K, T215Y, K219N, S251I, A272P, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.V35M+p.K43Q+p.D67G+p.S68G+p.K70R+p.V90I+p.K104N+p.I135T+p.E138Q+p.S162A+p.M184V+p.T215F+p.K219Q+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	65	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, V35M, K43Q, D67G, S68G, K70R, V90I, K104N, I135T, E138Q, S162A, M184V, T215F, K219Q, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D67N+p.T69D+p.K70R+p.L109LIM+p.K122E+p.M184V+p.V189VL+p.T200A+p.I202T+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.H221Y+p.L228H+p.L283I+p.R284K+p.T286TA+p.E297K	Missense mutation	41.8	Intermediate resistance	[21]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D67N, T69D, K70R, L109LIM, K122E, M184V, V189VL, T200A, I202T, H208Y, R211K, T215F, D218E, K219Q, H221Y, L228H, L283I, R284K, T286TA, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.E44D+p.D67N+p.K70R+p.L74I+p.L109LI+p.V118I+p.K122E+p.G190S+p.I202V+p.H208Y+p.L210W+p.R211K+p.T215X+p.T216X+p.K219E+p.L228H+p.M230MV	Missense mutation	21.7	Low-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, E44D, D67N, K70R, L74I, L109LI, V118I, K122E, G190S, I202V, H208Y, L210W, R211K, T215X, T216X, K219E, L228H, M230MV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.K43KE+p.D67N+p.R83K+p.D123E+p.T139K+p.M184V+p.I202V+p.T215Y+p.D250E+p.A272P+p.E297A+p.E300Q	Missense mutation	17	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, K43KE, D67N, R83K, D123E, T139K, M184V, I202V, T215Y, D250E, A272P, E297A, E300Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.K43Q+p.V75A+p.A98S+p.K101S+p.K102R+p.D121Y+p.K122E+p.I135T+p.V179E+p.Y181C+p.M184V+p.T200A+p.L210W+p.T215Y+p.K223Q+p.L228R+p.V245M+p.A272P+p.K275R+p.K277R+p.P294T	Missense mutation	11	Potential low-level resistance	[7]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, K43Q, V75A, A98S, K101S, K102R, D121Y, K122E, I135T, V179E, Y181C, M184V, T200A, L210W, T215Y, K223Q, L228R, V245M, A272P, K275R, K277R, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41ML+p.K49R+p.V60I+p.D67N+p.K70R+p.R83K+p.V90I+p.A98S+p.I135V+p.R211TA+p.T215Y+p.D218DE+p.V245S	Missense mutation	76	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41ML, K49R, V60I, D67N, K70R, R83K, V90I, A98S, I135V, R211TA, T215Y, D218DE, V245S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35ILM+p.T39A+p.M41L+p.D67N+p.K70R+p.L74V+p.K82KN+p.L100I+p.K102Q+p.K103N+p.V118I+p.D121DE+p.K122E+p.A158S+p.E169D+p.M184V+p.T200A+p.H208HY+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219E+p.L228H+p.V245E+p.E297R+p.E298ED+p.L301M+p.Y318YF	Missense mutation	11	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35ILM, T39A, M41L, D67N, K70R, L74V, K82KN, L100I, K102Q, K103N, V118I, D121DE, K122E, A158S, E169D, M184V, T200A, H208HY, L210W, R211K, T215Y, D218E, K219E, L228H, V245E, E297R, E298ED, L301M, Y318YF.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.D67N+p.T69N+p.K70R+p.V106A+p.V118I+p.K122E+p.I135T+p.V179I+p.M184V+p.G190A+p.I202V+p.F214L+p.T215F+p.K219Q+p.E248EK+p.S251SN+p.V254VG	Missense mutation	29.4	Low-level resistance	[22]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, D67N, T69N, K70R, V106A, V118I, K122E, I135T, V179I, M184V, G190A, I202V, F214L, T215F, K219Q, E248EK, S251SN, V254VG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K49R+p.D67G+p.S68G+p.K70R+p.D121H+p.K122E+p.D123S+p.I135T+p.Q174R+p.P176T+p.I178M+p.Y181C+p.T200A+p.R211K+p.T215F+p.D218E+p.K219E+p.V245Q+p.L260I+p.K277R	Missense mutation	97	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K49R, D67G, S68G, K70R, D121H, K122E, D123S, I135T, Q174R, P176T, I178M, Y181C, T200A, R211K, T215F, D218E, K219E, V245Q, L260I, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K65R+p.S68G+p.K70R+p.V75I+p.F77L+p.K103N+p.Y115F+p.F116Y+p.K122E+p.I135T+p.Q151M+p.T200I+p.I202V+p.V245M+p.A272P+p.E297N+p.H315X	Missense mutation	100	High-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K65R, S68G, K70R, V75I, F77L, K103N, Y115F, F116Y, K122E, I135T, Q151M, T200I, I202V, V245M, A272P, E297N, H315X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39A+p.E40F+p.M41L+p.K43E+p.D67N+p.K103S+p.V118I+p.K122E+p.R172K+p.D177E+p.I178F+p.G190A+p.Q207N+p.L210W+p.R211K+p.T215Y+p.K219R+p.K238T+p.A272P+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39A, E40F, M41L, K43E, D67N, K103S, V118I, K122E, R172K, D177E, I178F, G190A, Q207N, L210W, R211K, T215Y, K219R, K238T, A272P, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39A+p.M41L+p.K43E+p.D67N+p.V118I+p.D123E+p.D177E+p.M184V+p.G196E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K223Q+p.A272P+p.T286A+p.E297R+p.K311R	Missense mutation	24	Low-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39A, M41L, K43E, D67N, V118I, D123E, D177E, M184V, G196E, E203K, Q207E, H208Y, L210W, R211K, T215Y, K223Q, A272P, T286A, E297R, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35Q+p.T39A+p.M41L+p.V60I+p.D67N+p.T69N+p.K70R+p.D123E+p.I135T+p.I142V+p.K166R+p.D177E+p.G196E+p.E203D+p.T215F+p.K219E	Missense mutation	100	High-level resistance	[15]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35Q, T39A, M41L, V60I, D67N, T69N, K70R, D123E, I135T, I142V, K166R, D177E, G196E, E203D, T215F, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.D67N+p.T69D+p.K70R+p.L100LI+p.K102R+p.K103N+p.I135L+p.E169D+p.K173A+p.Q174K+p.I178L+p.Q207A+p.R211K+p.T215F+p.K219Q+p.L228H+p.V245Q+p.E248D+p.A272P+p.T286A+p.E291D+p.V292I+p.E297T	Missense mutation	100	High-level resistance	[5]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, D67N, T69D, K70R, L100LI, K102R, K103N, I135L, E169D, K173A, Q174K, I178L, Q207A, R211K, T215F, K219Q, L228H, V245Q, E248D, A272P, T286A, E291D, V292I, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.V60I+p.D67N+p.V118I+p.K122E+p.I135V+p.S162SN+p.M184V+p.T200A+p.E203ED+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219KE+p.V245Q+p.A272P+p.L283I+p.T286A+p.E291D+p.V292I+p.A304E	Missense mutation	20	Low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, V60I, D67N, V118I, K122E, I135V, S162SN, M184V, T200A, E203ED, Q207E, L210W, R211K, T215Y, K219KE, V245Q, A272P, L283I, T286A, E291D, V292I, A304E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39K+p.M41L+p.K43Q+p.V60I+p.D67N+p.K70R+p.V118I+p.D121H+p.K122E+p.I135V+p.K173T+p.Q174K+p.D177E+p.M184V+p.T200A+p.Q207E+p.R211RK+p.F214L+p.T215F+p.K219E+p.V245Q+p.E248D+p.D250E+p.A272P+p.K275R+p.Q278N+p.T286A+p.A288V+p.V292I+p.E297A	Missense mutation	83	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39K, M41L, K43Q, V60I, D67N, K70R, V118I, D121H, K122E, I135V, K173T, Q174K, D177E, M184V, T200A, Q207E, R211RK, F214L, T215F, K219E, V245Q, E248D, D250E, A272P, K275R, Q278N, T286A, A288V, V292I, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39L+p.I50V+p.V60I+p.D67N+p.K70R+p.K122E+p.D123S+p.I135T+p.K173S+p.Q174K+p.D177E+p.V179I+p.T200K+p.Q207A+p.R211S+p.F214L+p.K219Q+p.V245Q+p.T286A+p.E291D	Missense mutation	10	Potential low-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39L, I50V, V60I, D67N, K70R, K122E, D123S, I135T, K173S, Q174K, D177E, V179I, T200K, Q207A, R211S, F214L, K219Q, V245Q, T286A, E291D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VA+p.V60VI+p.D67N+p.K70R+p.A98AG+p.V106A+p.K122D+p.I135T+p.M184V+p.T200TA+p.I202V+p.E203X+p.F214FL+p.K219Q+p.K223Q+p.E224ED+p.F227L+p.A272P+p.K275Q+p.V276T+p.K281R+p.E297K	Missense mutation	13	Potential low-level resistance	[3]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VA, V60VI, D67N, K70R, A98AG, V106A, K122D, I135T, M184V, T200TA, I202V, E203X, F214FL, K219Q, K223Q, E224ED, F227L, A272P, K275Q, V276T, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.M41L+p.D67N+p.A98S+p.K122KE+p.I135T+p.M184V+p.E203K+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.V245T+p.A272P+p.K277R+p.I293V+p.E297K	Missense mutation	29.2	Low-level resistance	[12]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, M41L, D67N, A98S, K122KE, I135T, M184V, E203K, Q207E, L210W, R211RK, T215Y, V245T, A272P, K277R, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.M41L+p.D67N+p.S105A+p.K122E+p.I135T+p.M184V+p.L210W+p.R211K+p.T215Y+p.K238T+p.T253TP+p.I293V+p.E297R	Missense mutation	12	Potential low-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, M41L, D67N, S105A, K122E, I135T, M184V, L210W, R211K, T215Y, K238T, T253TP, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.M41L+p.K49KR+p.R83K+p.A98AG+p.K103KN+p.K122E+p.I135T+p.K166KR+p.D177E+p.M184V+p.I202V+p.Q207E+p.L210LW+p.R211RK+p.T215Y+p.A272P+p.E297A	Missense mutation	91	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, M41L, K49KR, R83K, A98AG, K103KN, K122E, I135T, K166KR, D177E, M184V, I202V, Q207E, L210LW, R211RK, T215Y, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K20R+p.V35I+p.K43KQ+p.D67N+p.R83K+p.V106A+p.K122KE+p.I178L+p.H208HY+p.L210W+p.R211RK+p.T215Y+p.F227L+p.V245E+p.K277R+p.T286A+p.D324DE+p.M357T+p.S379G+p.T386I+p.K390R+p.A400T	Missense mutation	100	High-level resistance	[20]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K20R, V35I, K43KQ, D67N, R83K, V106A, K122KE, I178L, H208HY, L210W, R211RK, T215Y, F227L, V245E, K277R, T286A, D324DE, M357T, S379G, T386I, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VIM+p.K13KN+p.V35VIM+p.T39TA+p.M41L+p.K43KQ+p.E44ED+p.D67N+p.L74LI+p.D86NS+p.K101X+p.K103KN+p.V118I+p.I135IV+p.E138EQ+p.S162X+p.K173KE+p.M184MV+p.G190GA+p.G196GE+p.L210W+p.R211K+p.T215Y+p.K219KN+p.H221X+p.Q278H+p.T286A+p.I293V+p.E297KR	Missense mutation	85	High-level resistance	[4]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VIM, K13KN, V35VIM, T39TA, M41L, K43KQ, E44ED, D67N, L74LI, D86NS, K101X, K103KN, V118I, I135IV, E138EQ, S162X, K173KE, M184MV, G190GA, G196GE, L210W, R211K, T215Y, K219KN, H221X, Q278H, T286A, I293V, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.S68G+p.T69K+p.K70G+p.V75I+p.F77L+p.Y115F+p.F116Y+p.K122E+p.Q151M+p.K166R+p.D177E+p.M184V+p.R211K+p.K277R+p.I293V+p.E297K	Missense mutation	100	High-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, S68G, T69K, K70G, V75I, F77L, Y115F, F116Y, K122E, Q151M, K166R, D177E, M184V, R211K, K277R, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.V75I+p.F77L+p.K102Q+p.F116Y+p.Q151M+p.S162C+p.M184V+p.K277R	Missense mutation	100	High-level resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, V75I, F77L, K102Q, F116Y, Q151M, S162C, M184V, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69N+p.K70R+p.V90I+p.K103N+p.V118I+p.I135M	Missense mutation	50	Intermediate resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69N, K70R, V90I, K103N, V118I, I135M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40F+p.M41L+p.K43E+p.D67N+p.V108I+p.V118I+p.K122E+p.D123E+p.Y181C+p.L210W+p.R211K+p.T215Y+p.K219N+p.A272P+p.T286A	Missense mutation	100	High-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40F, M41L, K43E, D67N, V108I, V118I, K122E, D123E, Y181C, L210W, R211K, T215Y, K219N, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.R83K+p.A98S+p.G196E+p.T200I+p.Q207E+p.L210W+p.R211RK+p.T215Y+p.V245M+p.K277R+p.T286A+p.L289X+p.I293IK+p.P294PT+p.E297K	Missense mutation	22	Low-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, R83K, A98S, G196E, T200I, Q207E, L210W, R211RK, T215Y, V245M, K277R, T286A, L289X, I293IK, P294PT, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.V21I+p.V35VI+p.M41L+p.K43E+p.E44A+p.T69D+p.R83K+p.K101Q+p.K103R+p.V108I+p.L109I+p.V118I+p.K122E+p.R172K+p.D177E+p.V179F+p.Y181C+p.M184MV+p.G190A+p.G196E+p.L210W+p.R211K+p.T215Y+p.D218E+p.K219R+p.L228R+p.T286P+p.A288S+p.I293V+p.E297R	Missense mutation	100	High-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, V21I, V35VI, M41L, K43E, E44A, T69D, R83K, K101Q, K103R, V108I, L109I, V118I, K122E, R172K, D177E, V179F, Y181C, M184MV, G190A, G196E, L210W, R211K, T215Y, D218E, K219R, L228R, T286P, A288S, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35L+p.T39A+p.E40F+p.M41L+p.K43E+p.D67N+p.T69D+p.V118I+p.K122P+p.D177E+p.I178IM+p.M184V+p.G196E+p.H208YF+p.L210W+p.R211K+p.T215Y+p.M230MV+p.V245T+p.Q278E+p.T296S+p.E297K	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35L, T39A, E40F, M41L, K43E, D67N, T69D, V118I, K122P, D177E, I178IM, M184V, G196E, H208YF, L210W, R211K, T215Y, M230MV, V245T, Q278E, T296S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35VI+p.K70R+p.K103KN+p.I135T+p.K166R+p.Y181YC+p.V189VI+p.G190GA+p.T215TA+p.L228LH+p.A272P+p.T286A+p.E297EQ	Missense mutation	27	Low-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35VI, K70R, K103KN, I135T, K166R, Y181YC, V189VI, G190GA, T215TA, L228LH, A272P, T286A, E297EQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.D67N+p.T69N+p.K70R+p.K104N+p.D123E+p.Q207QP+p.R211K+p.T215F+p.K219Q+p.T286A	Missense mutation	100	High-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, D67N, T69N, K70R, K104N, D123E, Q207QP, R211K, T215F, K219Q, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43N+p.E44D+p.D67N+p.V118I+p.K122E+p.S162X+p.K166R+p.V179VI+p.T200I+p.L210W+p.R211K+p.T215Y	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43N, E44D, D67N, V118I, K122E, S162X, K166R, V179VI, T200I, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.E44D+p.D67N+p.V118I+p.K122E+p.I135T+p.D177E+p.M184V+p.H208Y+p.L210W+p.R211N+p.T215Y+p.K219N+p.L283I+p.R284K+p.I293V+p.E297K+p.A304G	Missense mutation	100	High-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, E44D, D67N, V118I, K122E, I135T, D177E, M184V, H208Y, L210W, R211N, T215Y, K219N, L283I, R284K, I293V, E297K, A304G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35IL+p.T39AS+p.M41L+p.E44D+p.D67N+p.V90I+p.V106A+p.V118I+p.K122E+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219KR+p.K223E+p.F227L+p.K277R+p.Q278H+p.R284K+p.A288S+p.I293V	Missense mutation	100	High-level resistance	[22]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35IL, T39AS, M41L, E44D, D67N, V90I, V106A, V118I, K122E, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219KR, K223E, F227L, K277R, Q278H, R284K, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64H+p.D67N+p.T69N+p.K70R+p.V90I+p.K103N+p.V118I+p.I135M+p.T215E+p.K219Q+p.A272P	Missense mutation	100	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64H, D67N, T69N, K70R, V90I, K103N, V118I, I135M, T215E, K219Q, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.T69S_SA+p.K102Q+p.S162C+p.L210W+p.T215Y+p.K277R	Missense mutation	100	High-level resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, T69S_SA, K102Q, S162C, L210W, T215Y, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67E+p.T69S_SG+p.K70R+p.V75M+p.K122KE+p.I135T+p.E138A+p.P157PA+p.S162CY+p.P170PL+p.G190Q+p.H208Y+p.R211K+p.T215Y+p.A272P+p.K277R+p.V317A	Missense mutation	100	High-level resistance	[12]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67E, T69S_SG, K70R, V75M, K122KE, I135T, E138A, P157PA, S162CY, P170PL, G190Q, H208Y, R211K, T215Y, A272P, K277R, V317A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.K102Q+p.S162C+p.T215Y+p.K219Q+p.K277R	Missense mutation	54.6	Intermediate resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, K102Q, S162C, T215Y, K219Q, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.T69D+p.V75T+p.V118I+p.K122E+p.D123E+p.Q207E+p.L210W+p.T215Y+p.V245A+p.Q278H+p.I293V	Missense mutation	26	Low-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, T69D, V75T, V118I, K122E, D123E, Q207E, L210W, T215Y, V245A, Q278H, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.V106M+p.K122E+p.I135V+p.D177N+p.I178M+p.V179E+p.T200A+p.Q207E+p.T215Y+p.V245T+p.E248D+p.V261VG+p.N265ND+p.Q269QE+p.I270IL+p.A272P+p.I274IV+p.K277R	Missense mutation	100	High-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, V106M, K122E, I135V, D177N, I178M, V179E, T200A, Q207E, T215Y, V245T, E248D, V261VG, N265ND, Q269QE, I270IL, A272P, I274IV, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K102Q+p.S162C+p.T215Y+p.K277R	Missense mutation	11.2	Potential low-level resistance	[17]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K102Q, S162C, T215Y, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.D67N+p.T69D+p.W88C+p.V118I+p.K122KE+p.I135V+p.I142V+p.D177E+p.I178M+p.M184V+p.G196E+p.T200A+p.E203D+p.H208Y+p.L210W+p.R211N+p.T215Y	Missense mutation	100	High-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, D67N, T69D, W88C, V118I, K122KE, I135V, I142V, D177E, I178M, M184V, G196E, T200A, E203D, H208Y, L210W, R211N, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K64H+p.D67N+p.T69D+p.V75T+p.V118I+p.K122E+p.D123E+p.Q207E+p.L210W+p.T215Y+p.V245A+p.Q278H+p.I293V	Missense mutation	100	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K64H, D67N, T69D, V75T, V118I, K122E, D123E, Q207E, L210W, T215Y, V245A, Q278H, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.T69S_SS+p.A98G+p.K103N+p.I135L+p.E138A+p.Q197E+p.H208HY+p.L210W+p.R211X+p.T215Y+p.A272P	Missense mutation	100	High-level resistance	[14]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, T69S_SS, A98G, K103N, I135L, E138A, Q197E, H208HY, L210W, R211X, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.S68G+p.V90I+p.K122E+p.I135T+p.K166KR+p.R211K+p.T215SY+p.V245T+p.A272P+p.K277R+p.G285X+p.A288AS+p.E297KR	Missense mutation	72	High-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, S68G, V90I, K122E, I135T, K166KR, R211K, T215SY, V245T, A272P, K277R, G285X, A288AS, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60VI+p.D67DN+p.R143RT+p.G196GE+p.E203D+p.H208HY+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277KR+p.I293V	Missense mutation	100	High-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60VI, D67DN, R143RT, G196GE, E203D, H208HY, L210W, R211K, T215Y, A272P, K277KR, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60VI+p.D67N+p.K70R+p.V75X+p.D123E+p.S162SN+p.G196E+p.Q207H+p.T215F+p.K219E+p.A272P+p.Q278H+p.E297K	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60VI, D67N, K70R, V75X, D123E, S162SN, G196E, Q207H, T215F, K219E, A272P, Q278H, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.D67N+p.K70R+p.K103N+p.D121H+p.K122E+p.D177E+p.M184V+p.G196D+p.Q197K+p.L210LW+p.R211RK+p.T215F+p.D218E+p.K219EG+p.L228LH+p.A272P+p.L283I+p.E297K	Missense mutation	27	Low-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, D67N, K70R, K103N, D121H, K122E, D177E, M184V, G196D, Q197K, L210LW, R211RK, T215F, D218E, K219EG, L228LH, A272P, L283I, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.T69S_SG+p.V75M+p.K122E+p.R172K+p.I178M+p.Y188L+p.Q207E+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R+p.K281*L+p.T286A+p.A288AG+p.V292I	Missense mutation	100	High-level resistance	[19]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, T69S_SG, V75M, K122E, R172K, I178M, Y188L, Q207E, L210W, R211K, T215Y, A272P, K277R, K281*L, T286A, A288AG, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4PT+p.K20KR+p.V35I+p.T39S+p.M41L+p.E44D+p.S68SG+p.V118I+p.I135T+p.E194K+p.G196E+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H+p.V245K	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4PT, K20KR, V35I, T39S, M41L, E44D, S68SG, V118I, I135T, E194K, G196E, Q207E, L210W, R211K, T215Y, L228H, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K11KT+p.T39TA+p.M41L+p.V60I+p.D67N+p.K70R+p.V75VIM+p.V90I+p.K103X+p.V108VI+p.V118I+p.D123E+p.S162D+p.V179I+p.Y181C+p.I195L+p.L210W+p.R211T+p.T215Y+p.D218E+p.K219E+p.L228H+p.Q242X+p.A272P+p.K281R+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K11KT, T39TA, M41L, V60I, D67N, K70R, V75VIM, V90I, K103X, V108VI, V118I, D123E, S162D, V179I, Y181C, I195L, L210W, R211T, T215Y, D218E, K219E, L228H, Q242X, A272P, K281R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.Q207E+p.R211K+p.T215Y+p.T286A	Missense mutation	15	Low-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, Q207E, R211K, T215Y, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3A+p.K20KR+p.V35I+p.T39A+p.M41L+p.K43Q+p.E44D+p.D67N+p.T69AD+p.A98AG+p.V118I+p.K122E+p.I135T+p.V179VI+p.M184MV+p.Y188L+p.G196E+p.T200A+p.E203K+p.Q207E+p.H208FY+p.L210W+p.R211K+p.T215Y+p.K219KR+p.H221HL+p.K223Q+p.V245E	Missense mutation	100	High-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3A, K20KR, V35I, T39A, M41L, K43Q, E44D, D67N, T69AD, A98AG, V118I, K122E, I135T, V179VI, M184MV, Y188L, G196E, T200A, E203K, Q207E, H208FY, L210W, R211K, T215Y, K219KR, H221HL, K223Q, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.D67N+p.T69N+p.K70R+p.K103R+p.I135L+p.F214L+p.K219Q+p.A272P	Missense mutation	80	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, D67N, T69N, K70R, K103R, I135L, F214L, K219Q, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40F+p.M41L+p.K43E+p.N54I+p.D67N+p.S68G+p.V75M+p.W88C+p.G190A+p.R211K+p.T215Y+p.K219Q+p.K223Q	Missense mutation	100	High-level resistance	[26]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40F, M41L, K43E, N54I, D67N, S68G, V75M, W88C, G190A, R211K, T215Y, K219Q, K223Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K64N+p.D67N+p.T69N+p.K70R+p.K103R+p.I135L+p.F214L+p.K219Q+p.A272P	Missense mutation	100	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K64N, D67N, T69N, K70R, K103R, I135L, F214L, K219Q, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39E+p.M41L+p.K43KE+p.E44D+p.D67N+p.V75M+p.E79D+p.A98G+p.K103R+p.V118I+p.K122E+p.I142V+p.K173E+p.G196GE+p.H208Y+p.L210W+p.R211K+p.T215Y+p.Q278H+p.T286A	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39E, M41L, K43KE, E44D, D67N, V75M, E79D, A98G, K103R, V118I, K122E, I142V, K173E, G196GE, H208Y, L210W, R211K, T215Y, Q278H, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.R83K+p.D121H+p.K122E+p.I135T+p.D177E+p.I178M+p.G196E+p.R211T+p.T215Y+p.A272P+p.K277R	Missense mutation	100	High-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, R83K, D121H, K122E, I135T, D177E, I178M, G196E, R211T, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.E28K+p.K32E+p.T39K+p.M41L+p.K43E+p.E44D+p.V60I+p.D67N+p.V75M+p.K103N+p.V111I+p.V118I+p.I142V+p.S162C+p.I178L+p.V179I+p.Y181C+p.G196E+p.E203D+p.E204Q+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221Y+p.V245T+p.A288S+p.I293V+p.E297K+p.E312EQ	Missense mutation	100	High-level resistance	[12]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, E28K, K32E, T39K, M41L, K43E, E44D, V60I, D67N, V75M, K103N, V111I, V118I, I142V, S162C, I178L, V179I, Y181C, G196E, E203D, E204Q, H208Y, L210W, R211K, T215Y, H221Y, V245T, A288S, I293V, E297K, E312EQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.T39A+p.M41L+p.K43E+p.E44A+p.T69D+p.L74V+p.A98S+p.K103N+p.V111I+p.V118I+p.K122E+p.I135T+p.K166KR+p.I178IL+p.V179IT+p.Y181C+p.M184V+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.L228R+p.Q242L+p.I257L+p.A272P+p.K277R+p.T286A+p.I293V	Missense mutation	100	High-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, T39A, M41L, K43E, E44A, T69D, L74V, A98S, K103N, V111I, V118I, K122E, I135T, K166KR, I178IL, V179IT, Y181C, M184V, E203K, Q207E, H208Y, L210W, R211K, T215Y, K219R, K223Q, L228R, Q242L, I257L, A272P, K277R, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.T39A+p.M41L+p.S162C+p.Q207X+p.L210W+p.R211K+p.T215Y+p.V245K+p.L283I+p.I293V	Missense mutation	23	Low-level resistance	[24]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, T39A, M41L, S162C, Q207X, L210W, R211K, T215Y, V245K, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D67N+p.T69D+p.K70R+p.V106A+p.K122E+p.R125RK+p.V189L+p.T200A+p.I202T+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.H221Y+p.F227L+p.L228H+p.L283I+p.R284K+p.T286A+p.E297K	Missense mutation	100	High-level resistance	[21]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D67N, T69D, K70R, V106A, K122E, R125RK, V189L, T200A, I202T, H208Y, R211K, T215F, D218E, K219Q, H221Y, F227L, L228H, L283I, R284K, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.K49KR+p.D67N+p.K70R+p.D123DE+p.I135T+p.V179I+p.T215Y+p.D218E+p.K219Q+p.E248D+p.I293V+p.E297A	Missense mutation	100	High-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, K49KR, D67N, K70R, D123DE, I135T, V179I, T215Y, D218E, K219Q, E248D, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.M41L+p.K43Q+p.D67DA+p.V118VI+p.D121DH+p.K122KE+p.D123DE+p.I135V+p.D177E+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H+p.A272AP+p.T286A+p.E297EK	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, M41L, K43Q, D67DA, V118VI, D121DH, K122KE, D123DE, I135V, D177E, Q207E, L210W, R211K, T215Y, L228H, A272AP, T286A, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39A+p.M41L+p.K43E+p.E44D+p.D67N+p.V75M+p.A98G+p.K103N+p.V118I+p.K122E+p.I178L+p.H208HY+p.L210W+p.R211K+p.T215Y+p.A272P+p.T286A+p.V292I+p.E297EA	Missense mutation	100	High-level resistance	[9]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39A, M41L, K43E, E44D, D67N, V75M, A98G, K103N, V118I, K122E, I178L, H208HY, L210W, R211K, T215Y, A272P, T286A, V292I, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.M41L+p.K49R+p.V60I+p.D67N+p.T69N+p.K70R+p.V108I+p.K122Q+p.I135T+p.S162D+p.D177E+p.Y181C+p.M184V+p.V189I+p.T200A+p.T215F+p.D218E+p.K219Q+p.L228R+p.V245E+p.I293V	Missense mutation	100	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, M41L, K49R, V60I, D67N, T69N, K70R, V108I, K122Q, I135T, S162D, D177E, Y181C, M184V, V189I, T200A, T215F, D218E, K219Q, L228R, V245E, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.M41L+p.K49R+p.V60I+p.K64H+p.D67N+p.T69N+p.K70R+p.V108I+p.K122Q+p.I135T+p.S162D+p.D177E+p.Y181C+p.M184V+p.V189I+p.T200A+p.T215F+p.D218E+p.K219Q+p.L228R+p.V245E	Missense mutation	100	High-level resistance	[23]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, M41L, K49R, V60I, K64H, D67N, T69N, K70R, V108I, K122Q, I135T, S162D, D177E, Y181C, M184V, V189I, T200A, T215F, D218E, K219Q, L228R, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.V75M+p.I94L+p.K103KN+p.K122E+p.I135T+p.V179I+p.Y181C+p.M184V+p.H208Y+p.T215F+p.D218E+p.K219W+p.L228H+p.V245E+p.E248Q+p.A272P+p.A288S+p.V292I+p.I293V	Missense mutation	100	High-level resistance	[13]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, V60I, D67N, T69D, K70R, V75M, I94L, K103KN, K122E, I135T, V179I, Y181C, M184V, H208Y, T215F, D218E, K219W, L228H, V245E, E248Q, A272P, A288S, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39A+p.M41L+p.E44D+p.N54I+p.K64H+p.D67N+p.K70R+p.L74V+p.K101Q+p.K103R+p.V118I+p.K122E+p.D123E+p.I135V+p.S162D+p.R172K+p.Y181C+p.G196E+p.E203D+p.L210W+p.T215Y+p.D218E+p.K219Q+p.L228H+p.V245T+p.I293V	Missense mutation	100	High-level resistance	[25]

Resistant Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39A, M41L, E44D, N54I, K64H, D67N, K70R, L74V, K101Q, K103R, V118I, K122E, D123E, I135V, S162D, R172K, Y181C, G196E, E203D, L210W, T215Y, D218E, K219Q, L228H, V245T, I293V.

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

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Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.K101KE+p.V106VI+p.D123E+p.I135T+p.K173T+p.Q174K+p.D177E+p.Y181I+p.M184V+p.G196E+p.T200K+p.Q207A+p.P243T+p.V245K+p.D256E+p.A272P+p.E291D+p.V292I+p.I293V+p.E297A	Missense mutation	0.2	Susceptible	[27]
p.V8VI+p.K20KR+p.V35I+p.D123E+p.I135T+p.K173T+p.Q174K+p.D177E+p.V179VD+p.G196E+p.T200K+p.Q207A+p.P243T+p.V245K+p.D256E+p.A272AP+p.T286TA+p.E291D+p.V292I+p.I293V+p.E297A	Missense mutation	0.3	Susceptible	[27]
p.T39S+p.S48T+p.V60I+p.S68G+p.K102Q+p.K122KE+p.D123X+p.A158S+p.I178L+p.V179DE+p.I195IL+p.T200TA+p.R211K+p.I244V+p.V245E+p.A272P+p.K277KR+p.R284K+p.A288S+p.V292I+p.I293V+p.E297A	Missense mutation	0.5	Susceptible	[27]
p.S48T+p.V90VI+p.K101E+p.Y115YF+p.Y181YC+p.M184I+p.R211N+p.K219KE+p.V245N+p.L283I+p.V292I+p.I293V	Missense mutation	0.6	Susceptible	[27]
p.S48T+p.R211N+p.V245N+p.L283I+p.V292I+p.I293V	Missense mutation	0.8	Susceptible	[27]
p.V35E+p.T39A+p.E138K+p.D177E+p.V179VI+p.Y181I+p.M184V+p.E194EK+p.G196EK+p.Q207E+p.R211K+p.V245K+p.I257L+p.K277R+p.T286A+p.I293V	Missense mutation	0.2	Susceptible	[27]
p.V35E+p.T39A+p.D177E+p.G196E+p.Q207E+p.R211K+p.V245K+p.I257L+p.K277R+p.T286A+p.I293V	Missense mutation	0.6	Susceptible	[27]
p.V35T+p.T39TS+p.E40ED+p.V60I+p.V90VI+p.I135V+p.E138K+p.S162A+p.K173T+p.Q174E+p.D177E+p.I178M+p.M184I+p.T200A+p.E204EK+p.Q207E+p.R211K+p.H221HY+p.V245Q+p.A272P+p.K277R+p.K281R+p.T286A+p.I293V+p.P294T	Missense mutation	0.4	Susceptible	[27]
p.V35T+p.E40ED+p.V60I+p.K122KE+p.I135V+p.S162A+p.K173T+p.Q174EK+p.P176PS+p.D177E+p.I178M+p.T200A+p.E204EK+p.Q207E+p.R211K+p.V245Q+p.A272P+p.K277R+p.K281R+p.T286A+p.I293V+p.P294T	Missense mutation	0.6	Susceptible	[27]
p.V35M+p.V60I+p.R83K+p.K122E+p.I135T+p.E138K+p.S162H+p.M184I+p.V189VI+p.H221HY+p.E248D+p.A272P+p.E297K	Missense mutation	0.3	Susceptible	[27]
p.V35M+p.K49KR+p.V60I+p.R83K+p.K122E+p.I135T+p.S162H+p.E248D+p.A272P+p.E297K	Missense mutation	0.8	Susceptible	[27]
p.V35I+p.L74V+p.L100I+p.K103N+p.M184V+p.Q207A+p.R211K+p.P225H+p.I293V	Missense mutation	0.2	Susceptible	[27]
p.K11KR+p.V35I+p.R83K+p.T200TA+p.Q207A+p.R211K+p.I293V	Missense mutation	0.7	Susceptible	[27]
p.K70KE+p.V90I+p.A98S+p.K122E+p.E138K+p.A158S+p.S162A+p.D177E+p.M184I+p.G196E+p.R211S+p.A272S+p.T286A+p.P294T	Missense mutation	0.4	Susceptible	[27]
p.A98S+p.K122E+p.A158AS+p.I159IV+p.S162SA+p.D177E+p.G196E+p.R211S+p.A272S+p.T286A+p.P294T	Missense mutation	0.6	Susceptible	[27]
p.K20KR+p.A62AV+p.K65KN+p.V108VI+p.F171Y+p.D177E+p.I178IMV+p.M184MIV+p.T200A+p.Q207T+p.R211K+p.F227FC+p.L228LR+p.M230MI+p.L234LI+p.V245E+p.A272P	Missense mutation	0.1	Susceptible	[27]
p.F171Y+p.D177E+p.I178IMV+p.T200A+p.Q207T+p.R211K+p.V245E+p.A272P+p.K277KR	Missense mutation	0.8	Susceptible	[27]
p.V35I+p.V90I+p.K122E+p.D123N+p.I135L+p.S162C+p.Q174K+p.V179I+p.Y181I+p.M184V+p.G196E+p.A272P+p.A288T	Missense mutation	0.2	Susceptible	[27]
p.V35I+p.K122E+p.D123N+p.I135L+p.S162C+p.K173N+p.Q174K+p.V179I+p.G196E+p.A272P+p.A288T	Missense mutation	0.5	Susceptible	[27]
p.V35I+p.G45GE+p.V90VI+p.K122P+p.D123EK+p.I135L+p.T139I+p.D177AE+p.M184I+p.I195IK+p.K238N+p.A272P+p.A288G+p.I293V+p.P294T	Missense mutation	0.2	Susceptible	[27]
p.E6ED+p.V8VI+p.V35I+p.D86DE+p.K122P+p.D123E+p.I135L+p.T139I+p.D177E+p.Q207QK+p.R211RK+p.A272P+p.T286A+p.A288AG+p.I293IV	Missense mutation	0.6	Susceptible	[27]
p.K11T+p.V35I+p.E40K+p.K65R+p.T69T_X+p.I135L+p.D177E+p.I178M+p.V179VI+p.Y181C+p.Y188H+p.V189VI+p.G196E+p.K219KE+p.E291D+p.E297K	Missense mutation	0.4	Susceptible	[27]
p.V35I+p.R83K+p.D177E+p.G196E+p.H208HY+p.L210LF+p.R211K+p.E291D+p.E297K	Missense mutation	0.4	Susceptible	[27]
p.V35VL+p.V60I+p.K65KR+p.S68SG+p.I178L+p.M184MIV+p.G196E+p.E204EG+p.E248D+p.A272P+p.G273R+p.K275R+p.V292I+p.I293V	Missense mutation	0.4	Susceptible	[27]
p.K20KR+p.V60I+p.S68G+p.I178ILM+p.G196E+p.T200TA+p.E248D+p.A272P+p.G273R+p.K275R+p.K281KR+p.V292I+p.I293V	Missense mutation	0.8	Susceptible	[27]
p.V8I+p.K20R+p.A98AG+p.K101KE+p.K122KE+p.D123X+p.E138EK+p.I178IM+p.Y181YC+p.M184I+p.R211K+p.H221HY+p.V245I+p.E248ED+p.A272P+p.K277KR+p.I293V+p.P294S+p.E297R	Missense mutation	0.2	Susceptible	[27]
p.V8I+p.K20R+p.T69TA+p.K122KE+p.D123X+p.I178IM+p.R211K+p.V245VI+p.E248ED+p.A272P+p.K277R+p.I293V+p.P294S+p.E297R	Missense mutation	0.4	Susceptible	[27]
p.K82R+p.R83K+p.E138KQ+p.K173Q+p.I178M+p.M184I+p.T200A+p.Q207E+p.R211K+p.L228I+p.A272P+p.K277R+p.I293V+p.T296S+p.E297A	Missense mutation	0.2	Susceptible	[27]
p.T39TA+p.T69TA+p.K82KR+p.R83RK+p.S162SN+p.P170PL+p.K173Q+p.I178M+p.T200A+p.Q207E+p.R211K+p.P225PL+p.L228LF+p.A272P+p.K277KR+p.L282LF+p.I293V+p.T296S+p.E297A	Missense mutation	0.6	Susceptible	[27]
p.V35I+p.D67G+p.A98S+p.K101E+p.K122E+p.D123G+p.Y181C+p.M184I+p.T200A+p.K219E+p.M230L+p.V245E+p.K277R+p.I293V+p.P294A+p.E297K	Missense mutation	0.5	Susceptible	[27]
p.V35I+p.L80LF+p.A98S+p.K122E+p.T128TA+p.K166KR+p.I178IL+p.R211K+p.V245E+p.K277R+p.I293V+p.P294A+p.E297K	Missense mutation	0.5	Susceptible	[27]
p.K20R+p.V35L+p.T39A+p.K122E+p.D123DN+p.I135R+p.K173E+p.Q174QR+p.D177E+p.V179I+p.Y181C+p.M184V+p.G196E+p.T200I+p.I202IV+p.R211K+p.K249KR+p.A288S+p.I293V	Missense mutation	0.2	Susceptible	[27]
p.K20R+p.V35L+p.T39A+p.R83RK+p.K122E+p.I135R+p.K173E+p.D177E+p.V179I+p.G196E+p.T200I+p.I202V+p.R211K+p.V245VE+p.A288S+p.I293V	Missense mutation	0.4	Susceptible	[27]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

GeneSeq assay; PhenoSense GT assay

Experiment for
Drug Resistance

Viral load assay

Mechanism Description

Amino acid changes in IN may contribute to drug resistance or sensitivity.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V106I+p.V118I+p.I135V+p.T165I+p.T200A+p.F214L+p.V245R+p.D250E+p.A272P	Missense mutation	0.8	Susceptible	[1]
p.P4S+p.D67DN+p.T69N+p.K70KR+p.V90I+p.L100LI+p.K122E+p.D123N+p.I135IL+p.S162C+p.D177E+p.V179I+p.M184I+p.Y188L+p.T200A+p.R211K+p.K219E+p.M230MIL+p.L234LI+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	0.6	Susceptible	[1]
p.V35I+p.R83K+p.S162A+p.I178IM+p.Q207E+p.I293V+p.E297A+p.L301M	Missense mutation	0.5	Susceptible	[1]
p.K64KR+p.K102KR+p.D123E+p.K173X+p.P176PAT+p.D177DE+p.I178L+p.M184MV+p.Q197QEK+p.Q207E+p.R211K+p.V245VI+p.E248D+p.A272P+p.E297K	Missense mutation	0.7	Susceptible	[1]
p.V35I+p.S48T+p.K101KR+p.K103N+p.V106VI+p.T139TA+p.P157PS+p.T200I+p.E203D+p.Q207E+p.R211RK+p.D250E+p.A272P+p.K275R+p.K277R+p.T286TA+p.I293IV	Missense mutation	0.5	Susceptible	[1]
p.K20R+p.V35M+p.T69D+p.K102Q+p.K103N+p.K122E+p.E138G+p.S162SC+p.I178L+p.Q207E+p.A272P+p.K277R+p.L283I+p.I293V+p.E297A	Missense mutation	0.4	Susceptible	[1]
p.K20R+p.K122E+p.R211K+p.V245M+p.G262GE+p.A272P+p.V292VI+p.I293IV+p.E297A	Missense mutation	0.8	Susceptible	[1]
p.V35T+p.M41L+p.K43KN+p.V60I+p.K122E+p.K173KQ+p.D177EG+p.V179VI+p.Y188L+p.G196E+p.R211RK+p.T215D+p.V245E+p.I257L+p.K277R+p.I293V+p.E297R	Missense mutation	0.7	Susceptible	[1]
p.P4S+p.E6D+p.K122E+p.D123N+p.I142V+p.S162C+p.D177E+p.I178L+p.R211K+p.A272P+p.I293V+p.E297R	Missense mutation	0.7	Susceptible	[1]
p.K20R+p.V60I+p.K103N+p.D123E+p.I142V+p.Y181C+p.G196E+p.T200A+p.R211K+p.K219E+p.I293IM	Missense mutation	0.4	Susceptible	[1]
p.E6K+p.V35I+p.T39TI+p.K102KQ+p.V106VI+p.K122E+p.I135IMV+p.I142M+p.S162C+p.R211K+p.K277R+p.A288S+p.I293V	Missense mutation	0.4	Susceptible	[1]
p.K20R+p.I50IV+p.R83K+p.K166KR+p.K173X+p.Q174K+p.D177DE+p.I178L+p.V189VI+p.Q197QK+p.H198GE+p.Q207E+p.R211K+p.V245EQ+p.D250E+p.A272P+p.K277R	Missense mutation	1	Susceptible	[1]
p.V35T+p.T39TR+p.K122KE+p.D177E+p.K281R+p.T286A+p.I293IV+p.E297K	Missense mutation	1	Susceptible	[1]
p.R83K+p.K101KE+p.K122E+p.D123DE+p.S162A+p.Q174G+p.G196E+p.T200A+p.Q207E+p.V245E+p.A272P+p.K277R+p.I293V	Missense mutation	1.4	Susceptible	[1]
p.E6ED+p.K32KR+p.D121DY+p.K122KE+p.I178M+p.Q207K+p.R211S+p.V245EK+p.K277KR+p.I293V+p.L301LM	Missense mutation	1.6	Susceptible	[1]
p.V35IL+p.I50IV+p.V60I+p.R83K+p.I135T+p.K173E+p.I202V+p.E204ED+p.Q207QH+p.R211RK+p.A272P+p.K281R+p.T286A+p.E291D+p.E297A	Missense mutation	0.8	Susceptible	[1]
p.K101KR+p.V118I+p.K122KE+p.D123E+p.I135T+p.R172K+p.K173KR+p.F214L+p.A272P+p.K277R+p.K281R+p.T286TA+p.E297K	Missense mutation	0.8	Susceptible	[1]
p.E6D+p.K43KR+p.I50IF+p.D67DN+p.K70KR+p.A98S+p.D123E+p.I142V+p.K166R+p.K173KR+p.M184V+p.T200I+p.R211K+p.K219KE+p.V245VM+p.K249KR+p.A272P+p.K277KR+p.I293V	Missense mutation	2.3	Susceptible	[1]
p.V90I+p.D123E+p.I202V+p.E204Q+p.Q207D+p.R211K+p.V245T+p.I293V+p.E297A	Missense mutation	1.9	Susceptible	[1]
p.K20R+p.A33G+p.V35I+p.V60I+p.D67N+p.T69N+p.K70R+p.K103N+p.T139M+p.M184V+p.K219Q+p.L228H+p.D237DN+p.K238KT+p.S251I+p.A272S+p.K277R+p.E297KR	Missense mutation	3	Susceptible	[1]
p.V35T+p.T39E+p.K46T+p.E53D+p.V60I+p.K64KR+p.K101Q+p.K104KR+p.K122E+p.D123S+p.I135K+p.K166R+p.K173A+p.D177E+p.T200A+p.Q207E+p.R211K+p.V245E+p.D250E+p.V254I+p.A272P+p.K277R+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	1	Susceptible	[1]
p.S48SL+p.W88S+p.D123E+p.K166R+p.I178L+p.M184V+p.I195L+p.Q207K+p.R211K+p.V245QR+p.K277GR+p.A288S+p.I293V+p.E297V	Missense mutation	0.2	Susceptible	[1]
p.K20R+p.V60I+p.K122E+p.D123NS+p.K166R+p.I178M+p.R199RK+p.V245VE+p.E248N+p.A272P+p.K277R+p.L283I+p.P294AV+p.E297K	Missense mutation	0.6	Susceptible	[1]
p.I135V+p.P176S+p.D177E+p.A272P+p.G285A+p.E297K	Missense mutation	0.9	Susceptible	[1]
p.K13KR+p.K122E+p.I135T+p.S162C+p.M184V+p.G196E+p.K277R+p.I293V+p.E297A	Missense mutation	0.3	Susceptible	[1]
p.G45GE+p.T84TA+p.W88WS+p.D123E+p.I142IV+p.K166KR+p.I178L+p.M184V+p.I195L+p.Q207K+p.R211K+p.V245Q+p.K277R+p.A288S+p.I293V+p.E297V	Missense mutation	0.3	Susceptible	[1]
p.I135RT+p.M184MV+p.R211K+p.V245K+p.A272AP+p.T286A+p.E297EK	Missense mutation	0.6	Susceptible	[1]
p.K49R+p.R83K+p.S162C+p.I202V+p.Q207E+p.R211K+p.T215D+p.A272P+p.I293V	Missense mutation	0.8	Susceptible	[1]
p.K49R+p.T69N+p.D121Y+p.K122E+p.D177E+p.Y181C+p.R211K+p.F214L+p.A272P+p.K277R+p.T286AV+p.I293L	Missense mutation	0.6	Susceptible	[1]
p.V35T+p.M41L+p.L80I+p.K122E+p.D177E+p.Y188L+p.H198E+p.T215D+p.V245VE+p.I257IL+p.K277R+p.A288AT+p.I293V+p.E297R	Missense mutation	0.8	Susceptible	[1]
p.T69TN+p.K101P+p.K103N+p.I135V+p.I142V+p.Q145V+p.M184V+p.T200A+p.V245N+p.E248D+p.A272P+p.K275R+p.I293IV	Missense mutation	0.3	Susceptible	[1]
p.K20R+p.V35M+p.T69D+p.K102Q+p.K122KE+p.E138EAG+p.S162C+p.I178L+p.M184MV+p.Q207E+p.A272P+p.K277R+p.L283I+p.I293V+p.E297A	Missense mutation	0.3	Susceptible	[1]
p.K22R+p.V35M+p.S162C+p.I178L+p.V179I+p.Y181C+p.M184V+p.Y188L+p.T200A+p.Q207E+p.V245E+p.A272P+p.K277R	Missense mutation	0.1	Susceptible	[1]
p.K20R+p.V60I+p.V90I+p.K101Q+p.K103N+p.V108I+p.I135V+p.I142V+p.D177E+p.M184I+p.T200I+p.R211K+p.T215D+p.A272P+p.V276I+p.L283I+p.I293V+p.E302EG	Missense mutation	0.4	Susceptible	[1]
p.P4S+p.K122KE+p.D123E+p.E169D+p.I178L+p.T200A+p.I202IV+p.R211K+p.L246LP+p.E248D+p.A272P+p.T286TA+p.V292VI+p.I293V	Missense mutation	0.6	Susceptible	[1]
p.V35VIL+p.K103N+p.K122E+p.K173Q+p.I178M+p.M184MV+p.T200TA+p.V245M+p.E248D+p.D250DE+p.T286A+p.A288S	Missense mutation	0.7	Susceptible	[1]
p.P4PH+p.K20R+p.T39A+p.L74LI+p.R83K+p.V106VI+p.V179VI+p.M184V+p.E224D+p.V245E+p.T286A+p.P294PS+p.E297A	Missense mutation	0.3	Susceptible	[1]
p.V60I+p.I135V+p.D177E+p.R211K+p.I244V+p.V245I+p.D250E+p.A272P+p.K277KR+p.E297V	Missense mutation	0.5	Susceptible	[1]
p.V35I+p.R83RK+p.T165TI+p.F171Y+p.T200A+p.I202V+p.V245M+p.A272P+p.K277R+p.T286A+p.P294Q+p.E297A	Missense mutation	0.6	Susceptible	[1]
p.I135V+p.Y181C+p.M184V+p.G196E+p.T200A+p.Q207E+p.R211K+p.T215Y+p.V245K+p.K277R+p.E291D+p.E297K	Missense mutation	0.4	Susceptible	[1]
p.I5IV+p.E6K+p.K20KR+p.E44ED+p.D67N+p.T69D+p.V90VI+p.K102Q+p.K103N+p.K104KR+p.V118I+p.K122E+p.I142IV+p.V179I+p.Y181C+p.Y188L+p.T200A+p.Q207N+p.R211K+p.F214L+p.H221Y+p.I293V	Missense mutation	0.6	Susceptible	[1]
p.V35VI+p.T39TA+p.V90VI+p.L100I+p.Y115F+p.K122E+p.A158S+p.E169D+p.V179VI+p.M184V+p.Y188L+p.G196D+p.T200TA+p.Q207K+p.R211RK+p.T215F+p.K219E+p.L228H+p.V245E	Missense mutation	0.5	Susceptible	[1]
p.K20KR+p.T27TS+p.V60I+p.R83K+p.K122KE+p.Q174L+p.I178IL+p.T200X+p.R211RK+p.V245E+p.L283LI+p.T286TA+p.I293V	Missense mutation	0.8	Susceptible	[1]
p.V8VI+p.V60I+p.I135V+p.I142IT+p.S162C+p.E204Q+p.R211K+p.V245E+p.E248D+p.A272P+p.K277R+p.E297V	Missense mutation	0.4	Susceptible	[1]
p.M41L+p.K43Q+p.V60I+p.D67N+p.T69D+p.L74LV+p.V75VIM+p.L100I+p.K103N+p.K122KE+p.I135L+p.E138G+p.I142V+p.I178L+p.M184V+p.G196E+p.Q207E+p.T215Y+p.V245E+p.A272P+p.V276VI+p.K277KR+p.T286A+p.E291A+p.I293V	Missense mutation	0.9	Susceptible	[1]
p.P4S+p.V35VA+p.S48E+p.K101KE+p.K103KN+p.E169A+p.K173E+p.I178IM+p.I180IL+p.T200TA+p.V245VI+p.A272P	Missense mutation	1	Susceptible	[1]
p.K122E+p.D123N+p.L210F+p.R211K+p.E248D+p.A272P+p.Q278H+p.I293V	Missense mutation	0.9	Susceptible	[1]
p.K20R+p.T69N+p.K70R+p.S162C+p.F214L+p.V292I	Missense mutation	2.4	Susceptible	[1]
p.V35I+p.K65KR+p.S68SG+p.K70X+p.D123E+p.I135V+p.D177E+p.M184V+p.G196K+p.T200TA+p.F214FL+p.K219KR+p.I244IV+p.V245VIM+p.E248ED+p.T286A+p.E297K	Missense mutation	0.2	Susceptible	[1]
p.I135T+p.T165TI+p.E169D+p.D177E+p.V179L+p.I202V+p.A272P+p.T296S+p.E297K	Missense mutation	0.3	Susceptible	[1]
p.K20R+p.V35I+p.K122KE+p.I135T+p.E138K+p.R172K+p.T200V+p.R211RK+p.F214L+p.T286A+p.V292I+p.E297K	Missense mutation	0.7	Susceptible	[1]
p.V60A+p.D67G+p.K103R+p.V118I+p.D123N+p.E138K+p.T165I+p.M184V+p.I202V+p.F214L+p.D250V+p.A272P+p.V276T+p.K277R+p.T286A+p.P294A	Missense mutation	0.5	Susceptible	[1]
p.K20R+p.V35L+p.A62AV+p.S68SG+p.K70KN+p.V75T+p.R83K+p.S162N+p.T165I+p.F214L+p.H221Y+p.V245Q+p.E248N+p.D250E+p.G262GR+p.A272P+p.Q278H+p.K281R+p.T286A	Missense mutation	0.5	Susceptible	[1]
p.V35VI+p.W88WC+p.I142IV+p.E169K+p.K173E+p.I178L+p.I195L+p.T200A+p.V245I+p.T286A+p.V292I+p.E297A	Missense mutation	0.4	Susceptible	[1]
p.S68G+p.K102Q+p.D123E+p.I135T+p.K166R+p.G196E+p.T200F+p.V245E+p.P294T+p.L295F	Missense mutation	0.8	Susceptible	[1]
p.K20R+p.K122E+p.A158S+p.F214L+p.V245E+p.R284K+p.T286A	Missense mutation	2.4	Susceptible	[1]
p.V35R+p.T39A+p.K102Q+p.K103N+p.D123E+p.I135T+p.I142V+p.S162C+p.M184V+p.T200A+p.P225H+p.V245T+p.A272P+p.I293V+p.E297K	Missense mutation	0.9	Susceptible	[1]
p.R83K+p.I159V+p.T200I+p.I202IV+p.Q207E+p.R211KQ+p.A272P+p.K277R	Missense mutation	0.9	Susceptible	[1]
p.K11R+p.K122E+p.R211K+p.A272P+p.K277R+p.E291D+p.I293V	Missense mutation	0.7	Susceptible	[1]
p.V35VI+p.S68G+p.A98S+p.K122E+p.I135V+p.Y181C+p.G190A+p.R211K+p.F214L+p.H221Y+p.V245E+p.D250E+p.A272P	Missense mutation	0.4	Susceptible	[1]
p.V35T+p.K49R+p.K104R+p.K122E+p.I135V+p.Y144F+p.D177E+p.I178M	Missense mutation	1	Susceptible	[1]
p.T69N+p.V90VI+p.L100I+p.K103N+p.D121H+p.K122E+p.S162C+p.M184I+p.T200A+p.E224D+p.L228R+p.A272P+p.K277R+p.I293V+p.E297EK+p.I329V+p.Q334E+p.G359S+p.K390R+p.A400T+p.V435I+p.S468P+p.T470A+p.L491S+p.Q547K+p.I556V+p.K558R	Missense mutation	0.2	Susceptible	[1]
p.V35I+p.K65R+p.R83K+p.K103N+p.K173N+p.Q174R+p.G190GA+p.R211K+p.K249KQ+p.A272P+p.K277Q+p.Q278K+p.K281KR+p.T286TA+p.E297Q	Missense mutation	0.7	Susceptible	[1]
p.V35VA+p.K49R+p.V60VI+p.D67N+p.T69TN+p.K70R+p.S105L+p.V106A+p.E138EG+p.M184V+p.Q207K+p.F214FL+p.K219E+p.F227L+p.V245T+p.D250S+p.A272P+p.I293V	Missense mutation	9.2	Susceptible	[1]
p.P1PS+p.K20R+p.V35L+p.K103N+p.S162C+p.I178L+p.Q207E+p.R211K+p.V245E+p.A272P+p.K277R	Missense mutation	0.9	Susceptible	[1]
p.K20R+p.V35I+p.V90VI+p.K101P+p.K103N+p.I135R+p.V189VI+p.T200TA+p.T215Y+p.V245E+p.R284K+p.T286A+p.P294T	Missense mutation	8.1	Susceptible	[1]
p.K20R+p.V60I+p.D67N+p.D123E+p.I135AV+p.I159V+p.D177E+p.M184V+p.I202V+p.R211K+p.K219Q+p.A272P	Missense mutation	0.4	Susceptible	[1]
p.K65R+p.L100I+p.K103N+p.I244V+p.V245M+p.D250E+p.A272P+p.K281R+p.T286A+p.E297K	Missense mutation	0.4	Susceptible	[1]
p.V106VI+p.K122E+p.K166KR+p.V179D+p.Y188C+p.Q207E+p.R211K+p.K277R+p.I293V	Missense mutation	0.4	Susceptible	[1]
p.T39TA+p.V60I+p.D67N+p.T69D+p.K70R+p.A98S+p.K101P+p.K103N+p.V118I+p.K122E+p.I135T+p.K166R+p.M184V+p.D192DN+p.I202V+p.F214L+p.T215V+p.K219Q+p.L228H+p.K277R+p.I293V+p.E297A	Missense mutation	1.7	Susceptible	[1]
p.M41L+p.D67N+p.V106M+p.K122E+p.I135V+p.D177N+p.I178M+p.V179E+p.T200A+p.Q207E+p.T215CY+p.V245T+p.E248D+p.A272P+p.K277R+p.E291D+p.E297K	Missense mutation	7.2	Susceptible	[1]
p.K20R+p.T39A+p.M41L+p.K43E+p.E44EA+p.D67N+p.L74V+p.K103NS+p.V118I+p.K122P+p.Y181C+p.M184V+p.G190GA+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223T+p.L228H+p.T253TA	Missense mutation	9.4	Susceptible	[1]
p.D67N+p.K70R+p.R83K+p.M184V+p.R211T	Missense mutation	1	Susceptible	[1]
p.I31L+p.V35T+p.M41L+p.A62AV+p.V75I+p.A98S+p.K103N+p.D123E+p.Q174QR+p.I178L+p.V179I+p.Y181YC+p.M184V+p.Q207E+p.R211K+p.T215Y+p.L228H	Missense mutation	7.6	Susceptible	[1]
p.D67N+p.K70R+p.I135T+p.S162C+p.Q174QE+p.Y181C+p.M184V+p.Q207KR+p.R211Q+p.K219Q+p.H221Y+p.I293V	Missense mutation	1.6	Susceptible	[1]
p.Y181C+p.T200TA+p.F214L+p.H221Y+p.E291D+p.E297K	Missense mutation	0.3	Susceptible	[1]
p.S48T+p.K104R+p.K122P+p.I135T+p.S191T+p.T200E+p.F214L+p.V245Q+p.A272P+p.K277KT+p.E297A+p.E302D	Missense mutation	1.1	Susceptible	[1]
p.D67G+p.S68G+p.K70R+p.K101Q+p.K122E+p.V179I+p.Y181I+p.I195T+p.Q207E+p.R211K+p.F214L+p.K219Q+p.H221Y+p.L228H+p.V245E+p.A272P+p.K277R+p.T286A+p.I293V	Missense mutation	3.6	Susceptible	[1]
p.K20KR+p.T27S+p.E28K+p.K32E+p.M41ML+p.V60I+p.T69DN+p.K70KR+p.R83K+p.K101E+p.V179I+p.Y181V+p.M184V+p.T200A+p.Q207D+p.T215F+p.L228R+p.V245E+p.T286A+p.E297K	Missense mutation	4.3	Susceptible	[1]
p.K30KR+p.T69S+p.I135V+p.K173E+p.M184V+p.P243S+p.V245K+p.K277R+p.I293V+p.S322T+p.A360T+p.K390R+p.T403I+p.K431T+p.V435A+p.T470S+p.I506L+p.A508G+p.Q509L+p.S519N+p.I556V	Missense mutation	0.2	Susceptible	[1]
p.R83K+p.K122E+p.D123G+p.E138A+p.K166R+p.T200E+p.R211K+p.V245E+p.A272P+p.V276I+p.K277R+p.L283I+p.I293V+p.E297K	Missense mutation	0.7	Susceptible	[1]
p.E28K+p.V35M+p.M41L+p.T69N+p.K70R+p.R83K+p.K101E+p.K122P+p.S162C+p.D177E+p.M184V+p.I195L+p.V276T+p.K277R+p.E297EK	Missense mutation	1.7	Susceptible	[1]
p.V35M+p.T39S+p.R83K+p.K122KE+p.D123E+p.E138A+p.I178IL+p.I202V+p.R211K+p.T286P+p.I293V+p.I329L+p.Q334E+p.M357T+p.A376T+p.E449D+p.D460N+p.S468P+p.H483N+p.L491P+p.Q524E+p.V559VI	Missense mutation	0.9	Susceptible	[1]
p.K20R+p.K101E+p.K122E+p.M184V+p.G190A+p.D250E+p.A272P+p.P294Q+p.E302D	Missense mutation	0.9	Susceptible	[1]
p.E6D+p.K103N+p.L109LS+p.D123E+p.I135T+p.S162A+p.M184IV+p.T200I+p.E204Q+p.Q207E+p.F214L+p.M230L+p.I244V+p.I293V	Missense mutation	0.8	Susceptible	[1]
p.K11KRT+p.V60VI+p.W88C+p.D121H+p.K122E+p.I135T+p.D177E+p.Y181C+p.R211K+p.H221Y+p.V245E+p.V254VI+p.A272P+p.A288G+p.E291D+p.I293IV+p.E297K	Missense mutation	0.4	Susceptible	[1]
p.E6D+p.K122E+p.E138A+p.I142T+p.D177E+p.I178M+p.I195L+p.I202V+p.R211K+p.V245I+p.A272P+p.L283I+p.I293V+p.E297A	Missense mutation	0.7	Susceptible	[1]
p.S162C+p.I178IM+p.R211K+p.V245VA+p.A272P+p.K277R	Missense mutation	0.5	Susceptible	[1]
p.A98S+p.D123E+p.I135IL+p.I142IV+p.D177E+p.I178M+p.M184V+p.G196E+p.T200R+p.R211G+p.V245E+p.A272P+p.A288AS+p.I293V	Missense mutation	0.6	Susceptible	[1]
p.K122E+p.I142V+p.T200I+p.Q207A+p.R211K+p.V245E+p.A272P	Missense mutation	1.2	Susceptible	[1]
p.A98G+p.K102R+p.K103N+p.I135L+p.M184V+p.T286A+p.A288P+p.E297K	Missense mutation	0.8	Susceptible	[1]
p.V35T+p.D123E+p.M184V+p.A272P+p.T286A+p.E297K	Missense mutation	0.5	Susceptible	[1]
p.P4S+p.E6D+p.K103N+p.I135T+p.M184V+p.R211K+p.V292I	Missense mutation	0.5	Susceptible	[1]
p.M16MV+p.T69TN+p.V90I+p.L100I+p.K103N+p.D123E+p.I135V+p.G196E+p.P243S+p.V245E+p.E248DV+p.I293V+p.E297R	Missense mutation	0.5	Susceptible	[1]
p.V35I+p.M41L+p.D67N+p.L74V+p.R83K+p.K103N+p.K122KE+p.E169D+p.Y181C+p.L210W+p.R211K+p.T215Y	Missense mutation	5.1	Susceptible	[1]
p.K20R+p.L74LV+p.Y115F+p.K122E+p.Y181C+p.M184V+p.R211K+p.F214L+p.H221Y+p.V241VM+p.I293V+p.E297A	Missense mutation	0.5	Susceptible	[1]
p.K11K*+p.K20R+p.K64R+p.D67G+p.K70R+p.K101P+p.K103N+p.D123E+p.R172K+p.M184V+p.Q207E+p.R211K+p.F214L+p.K219E+p.T253TS	Missense mutation	0.5	Susceptible	[1]
p.K11KR+p.K20R+p.K32KN+p.V35I+p.R83K+p.A98S+p.K102KQ+p.K122E+p.I135T+p.E169ED+p.T200A+p.I202V+p.V245E+p.E248D+p.D250E	Missense mutation	1.4	Susceptible	[1]
p.I31V+p.D67N+p.T69N+p.D123E+p.E138K+p.D177E+p.M184V+p.G196E+p.K219Q+p.D237E+p.K277R+p.T286TA+p.I293V+p.E297A	Missense mutation	1.4	Susceptible	[1]
p.R83K+p.K102R+p.S163T+p.K173N+p.Q174K+p.V179E+p.M184V+p.Q207E+p.R211K+p.V245T+p.A272P	Missense mutation	0.7	Susceptible	[1]
p.M41L+p.E44D+p.K122E+p.D123E+p.I135T+p.S162Y+p.N175Y+p.M184V+p.T200E+p.L210W+p.R211K+p.T215Y+p.L228LH	Missense mutation	9.1	Susceptible	[1]
p.K70KR+p.K122E+p.A158S+p.E169D+p.M184V+p.Y188L+p.G196D+p.Q207K+p.T215F+p.V245E	Missense mutation	4.1	Susceptible	[1]
p.E6D+p.L74V+p.V106I+p.I135IM+p.V179I+p.Y181I+p.Q197K+p.T200I+p.F214L+p.H221Y+p.E248D	Missense mutation	0.2	Susceptible	[1]
p.K20R+p.V60I+p.T69N+p.K70R+p.I142V+p.V179D+p.Y188L+p.R211K+p.V245I+p.K277R+p.A288S+p.I293V+p.E297A	Missense mutation	3.1	Susceptible	[1]
p.M16MV+p.M41L+p.L74V+p.L100I+p.K103N+p.K122E+p.I135T+p.T139TA+p.K173KR+p.I178L+p.R211K+p.T215Y+p.A272P+p.I293V+p.E297A	Missense mutation	4	Susceptible	[1]
p.K49KR+p.K102R+p.Y115F+p.K122E+p.I135V+p.I178V+p.M184V+p.T200A+p.R211K+p.H221Y+p.V245T+p.A272AS+p.V276I+p.L283I+p.I293V+p.E297A	Missense mutation	0.8	Susceptible	[1]
p.K101R+p.I135R+p.Q174H+p.M184V+p.G196GR+p.R211RK+p.T286A+p.T296S+p.E297K+p.A299AG	Missense mutation	0.2	Susceptible	[1]
p.T7A+p.M41L+p.K43E+p.D67N+p.K101Q+p.V106I+p.D123E+p.I135L+p.Y181I+p.M184V+p.Q197L+p.L210W+p.R211K+p.T215Y+p.A272P+p.I293V+p.L303P	Missense mutation	3.5	Susceptible	[1]
p.M16V+p.T39TA+p.M41L+p.L74V+p.R83K+p.L100I+p.K103N+p.D177E+p.M184V+p.T200A+p.R211Q+p.T215Y+p.H221Y+p.R284K+p.I293V+p.E297A	Missense mutation	0.6	Susceptible	[1]
p.V60I+p.D67G+p.S68G+p.K70KR+p.L74V+p.I135V+p.E138A+p.K173E+p.I178M+p.G190E+p.R211A+p.K219N+p.V245E+p.E248D+p.D250E+p.A272P+p.T286A	Missense mutation	1.4	Susceptible	[1]
p.I37L+p.M41L+p.A62V+p.S68G+p.V75T+p.K122E+p.D123N+p.I178M+p.M184V+p.T200A+p.E204D+p.Q207D+p.R211K+p.T215Y+p.A288S+p.I293V	Missense mutation	2.6	Susceptible	[1]
p.V35I+p.V60I+p.K64KR+p.D67N+p.K101R+p.V118I+p.K122E+p.I135T+p.Q174N+p.Q207E+p.R211K+p.F214L+p.T215V+p.K219Q+p.I293V	Missense mutation	1.8	Susceptible	[1]
p.K11KT+p.D123E+p.Q174K+p.D177E+p.M184V+p.Q207E+p.R211K+p.V245M+p.A272P+p.K277R+p.L283LR	Missense mutation	0.5	Susceptible	[1]
p.V35T+p.T69S+p.K70R+p.K104R+p.R211Q+p.K277R+p.Q278H+p.P294T	Missense mutation	3.5	Susceptible	[1]
p.K20R+p.V35IM+p.D67N+p.K70R+p.K122E+p.I135T+p.D177E+p.M184V+p.H208Y+p.R211K+p.T215Y+p.D218E+p.K219Q+p.I257L+p.A272P+p.A288S+p.I293V	Missense mutation	4.7	Susceptible	[1]
p.K64R+p.D67N+p.K70R+p.K122E+p.I135T+p.I178M+p.A272T+p.A288S+p.I293V+p.E297A	Missense mutation	6.6	Susceptible	[1]
p.K20R+p.S68G+p.L74V+p.V75T+p.V108I+p.V111I+p.Y115F+p.I135L+p.Q151M+p.S162SN+p.I178L+p.Y181C+p.G190A+p.F214L+p.H221Y+p.F227L+p.V245E+p.A272P+p.K277R+p.E297A	Missense mutation	5.2	Susceptible	[1]
p.K20KR+p.V60I+p.V106I+p.K122A+p.I135T+p.S162C+p.V179I+p.T200A+p.R211K+p.A272P+p.K277KR	Missense mutation	1	Susceptible	[1]
p.K166R+p.T200A+p.R211RK+p.F214L+p.V245M+p.V276T+p.K277R+p.I293V	Missense mutation	1	Susceptible	[1]
p.K122KE+p.T165I+p.Q207E	Missense mutation	0.5	Susceptible	[1]
p.M41L+p.R83RK+p.M184V+p.G196E+p.T200A+p.Q207K+p.R211A+p.T215Y+p.A272P+p.K277R+p.T286A+p.K287KN+p.E297KR	Missense mutation	4.4	Susceptible	[1]
p.M41L+p.L74V+p.I142V+p.T165L+p.E169D+p.Y181I+p.M184V+p.R211K+p.T215Y	Missense mutation	2.6	Susceptible	[1]
p.K20R+p.M41L+p.K43N+p.E44ED+p.D67N+p.L74I+p.K103N+p.V118I+p.K122E+p.V179I+p.Y181C+p.M184V+p.G196E+p.E204K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.N265NS+p.Q269QE+p.A272P+p.I293V+p.E297A+p.I329L+p.N348I+p.M357T+p.G359S+p.A376S+p.T386I+p.A400T+p.V435A+p.G436GE+p.D460N+p.V467I+p.S468P+p.H483HY+p.L491S+p.E492K	Missense mutation	2.7	Susceptible	[1]
p.K65R+p.D67N+p.S68N+p.K70G+p.R83K+p.K103N+p.D121H+p.K122E+p.D177E+p.G196D+p.Q197K+p.K219R+p.L228LP+p.A272P+p.L283I+p.E297K	Missense mutation	0.5	Susceptible	[1]
p.K70R+p.K103N+p.K122P+p.I135T+p.E169D+p.M184V+p.T200V+p.F214L+p.K238KT+p.A272P+p.K277R+p.I293V+p.E297K	Missense mutation	1.1	Susceptible	[1]
p.E6K+p.R72RG+p.K122E+p.D123S+p.I135T+p.D177E+p.M184V+p.G196E+p.F214L+p.N265ND+p.A272P+p.A288AG	Missense mutation	0.5	Susceptible	[1]
p.P4S+p.T7A+p.K20R+p.V35M+p.M41L+p.D67N+p.L74V+p.L100I+p.K102R+p.K103N+p.M184V+p.E203K+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K238KN	Missense mutation	1.7	Susceptible	[1]
p.V35I+p.D123E+p.D177E+p.M184V+p.T200I+p.I202V+p.R211K+p.K219KR+p.A272P+p.I293V	Missense mutation	0.2	Susceptible	[1]
p.D121H+p.K122E+p.I135IT+p.I142IV+p.D177E+p.I178M+p.M184V+p.A272P	Missense mutation	0.6	Susceptible	[1]
p.E6D+p.I135T+p.S162C+p.M184V+p.Q207E+p.F214FL+p.K277R	Missense mutation	0.4	Susceptible	[1]
p.M41L+p.S162C+p.E169D+p.M184V+p.T200A+p.R211K+p.T215Y	Missense mutation	1.6	Susceptible	[1]
p.V60I+p.R83K+p.K102Q+p.K277T+p.I293V+p.E297A+p.A304G	Missense mutation	0.9	Susceptible	[1]
p.K103N+p.K122E+p.I142V+p.M184V+p.R211K+p.Q269QH+p.I293V	Missense mutation	0.6	Susceptible	[1]
p.D67N+p.T69D+p.K70R+p.V118I+p.K122E+p.I135T+p.I180V+p.M184V+p.F214L+p.T215F+p.K219Q+p.A272AP	Missense mutation	2.6	Susceptible	[1]
p.A98S+p.K101E+p.D123E+p.E138A+p.I142V+p.V179I+p.G190A+p.T200I+p.V245MT+p.A272P	Missense mutation	1.4	Susceptible	[1]
p.K122E+p.I178M+p.M230MLV	Missense mutation	0.9	Susceptible	[1]
p.D67N+p.K122KE+p.I178L+p.Y188L+p.R211K+p.F214L+p.T215F+p.K219Q+p.K277R+p.T286A+p.A288G+p.E297K	Missense mutation	7.2	Susceptible	[1]
p.T39A+p.V111I+p.K122E+p.I135V+p.I142T+p.Q207E+p.F214L+p.L228R	Missense mutation	0.5	Susceptible	[1]
p.T69N+p.D123E+p.I142V+p.I180V+p.Y181C+p.G196E+p.T200A+p.E203K+p.L228LH+p.K277R+p.T286A+p.E297K	Missense mutation	0.3	Susceptible	[1]
p.K103N+p.I135V+p.S162C+p.I178L+p.M184V+p.R211K+p.L234LH+p.P243PH	Missense mutation	0.4	Susceptible	[1]
p.K102Q+p.V108I+p.D121Y+p.K122E+p.D177E+p.I178L+p.Y181C+p.T215D+p.V245K	Missense mutation	0.2	Susceptible	[1]
p.K11KR+p.V75T+p.S162C+p.M184V+p.T200A+p.E248V+p.N265NT+p.A272P+p.T286A	Missense mutation	0.3	Susceptible	[1]
p.V35K+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.L74V+p.K101E+p.K104N+p.V118I+p.K122E+p.I135T+p.S162D+p.Y181C+p.M184V+p.G190S+p.F214L+p.T215F+p.K219Q+p.A272P	Missense mutation	5.2	Susceptible	[1]
p.V35I+p.M41L+p.E44D+p.V75M+p.R83RK+p.A98AG+p.K103KN+p.K122E+p.I135IT+p.I142T+p.T165TA+p.I178L+p.Y181C+p.M184V+p.G190GR+p.G196DE+p.R199RIS+p.T200A+p.K201KQ+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219KI	Missense mutation	4.5	Susceptible	[1]
p.K11R+p.E53D+p.D67N+p.K70R+p.V108I+p.K122E+p.Y181C+p.T215I+p.K219E+p.H221HY+p.V245E	Missense mutation	7.1	Susceptible	[1]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Site-directed mutagenesis; Sanger sequencing assay

Experiment for
Drug Resistance

PhenoSense assay

Mechanism Description

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62AV+p.K122P+p.I178IM+p.M184V+p.A272P+p.K277R+p.T286A+p.V292IT+p.E297A	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62AV, K122P, I178IM, M184V, A272P, K277R, T286A, V292IT, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62AV+p.K65R+p.K102R+p.K103N+p.A158T+p.M184V+p.T200E+p.R211K+p.L234I+p.A272S+p.I293V+p.P294S	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62AV, K65R, K102R, K103N, A158T, M184V, T200E, R211K, L234I, A272S, I293V, P294S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.K65R+p.K122P+p.I135T+p.D177E+p.Y181C+p.M184V+p.G190X+p.R284RK+p.I293IV+p.E297EK	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, K65R, K122P, I135T, D177E, Y181C, M184V, G190X, R284RK, I293IV, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.V75I+p.D86E+p.W88WS+p.V90VI+p.K101P+p.K103N+p.K122P+p.I135T+p.D177E+p.D192N+p.T200A+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, V75I, D86E, W88WS, V90VI, K101P, K103N, K122P, I135T, D177E, D192N, T200A, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I135IT+p.I202V+p.R211K	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I135IT, I202V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.K70E+p.K102Q+p.K103KR+p.V106M+p.Y115F+p.D123E+p.I142V+p.S162C+p.E169D+p.K173Q+p.I178L+p.V179D+p.M184V+p.T200A+p.K277R+p.T286A+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, K70E, K102Q, K103KR, V106M, Y115F, D123E, I142V, S162C, E169D, K173Q, I178L, V179D, M184V, T200A, K277R, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.K70E+p.V90I+p.K102Q+p.I135T+p.S162F+p.I178IM+p.Y181C+p.T200E+p.R211QH+p.V245E+p.K277R+p.T286A+p.I293V+p.E297K	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, K70E, V90I, K102Q, I135T, S162F, I178IM, Y181C, T200E, R211QH, V245E, K277R, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.K70E+p.V90I+p.K122E+p.I135IT+p.S162F+p.I178IM+p.Y181C+p.T200E+p.R211HQ+p.V245E+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, K70E, V90I, K122E, I135IT, S162F, I178IM, Y181C, T200E, R211HQ, V245E, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67G+p.K70E+p.V90I+p.L100I+p.K103N+p.D121H+p.K122E+p.D123DE+p.I135R+p.K154KR+p.Q174N+p.D177E+p.M184V+p.T200E+p.R211K+p.L228R+p.A272S+p.K277R+p.T286A+p.A288T+p.I293IV+p.E297K	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67G, K70E, V90I, L100I, K103N, D121H, K122E, D123DE, I135R, K154KR, Q174N, D177E, M184V, T200E, R211K, L228R, A272S, K277R, T286A, A288T, I293IV, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EA+p.K49KR+p.K70E+p.K101H+p.Y115YF+p.S163ST+p.Y181C+p.M184V+p.R211G+p.L228R	Missense mutation	0.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EA, K49KR, K70E, K101H, Y115YF, S163ST, Y181C, M184V, R211G, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EK+p.D123E+p.I135IM+p.K166R+p.V179D+p.M184V+p.T200I+p.I202V+p.R211K+p.A272P+p.Q278E+p.L283I+p.E298A	Missense mutation	0.4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EK, D123E, I135IM, K166R, V179D, M184V, T200I, I202V, R211K, A272P, Q278E, L283I, E298A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28G+p.A98S+p.K102Q+p.P140PS+p.E169D+p.M184MV+p.E194K+p.I195L+p.G196E+p.T200A+p.R211K+p.V245M+p.A272P+p.K277R+p.L283I+p.I293V+p.E297KR	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28G, A98S, K102Q, P140PS, E169D, M184MV, E194K, I195L, G196E, T200A, R211K, V245M, A272P, K277R, L283I, I293V, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E29EK+p.A98S+p.K102KQ+p.K103KN+p.I178L+p.M184MIV+p.L193LF+p.G196E+p.Q197KN+p.T215TI+p.G231GS	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E29EK, A98S, K102KQ, K103KN, I178L, M184MIV, L193LF, G196E, Q197KN, T215TI, G231GS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E36EK+p.T39TA+p.E53D+p.T69X+p.K70KE+p.A98AS+p.K122KE+p.M184V+p.T200A+p.Q207E+p.R211RK	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E36EK, T39TA, E53D, T69X, K70KE, A98AS, K122KE, M184V, T200A, Q207E, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K11T+p.K32KR+p.V35T+p.T39K+p.K43E+p.V60I+p.S68G+p.K122E+p.D123S+p.K173I+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207A+p.R211S+p.T215TA+p.K238R	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K11T, K32KR, V35T, T39K, K43E, V60I, S68G, K122E, D123S, K173I, Q174K, D177E, I178M, T200A, Q207A, R211S, T215TA, K238R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.V8VI+p.K101KQ+p.K103N+p.K122KE+p.I135T+p.E138G+p.T200X+p.I202IV+p.Q207QK+p.F214L+p.K238T	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, V8VI, K101KQ, K103N, K122KE, I135T, E138G, T200X, I202IV, Q207QK, F214L, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EG+p.K30KR+p.V35T+p.T39E+p.S48E+p.D113DN+p.S162C+p.K173A+p.Q174K+p.D177E+p.I178L+p.I180IL+p.M184I+p.T200A+p.Q207E+p.R211K+p.V245Q+p.A272P+p.K277R+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EG, K30KR, V35T, T39E, S48E, D113DN, S162C, K173A, Q174K, D177E, I178L, I180IL, M184I, T200A, Q207E, R211K, V245Q, A272P, K277R, T286A, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.E28EA+p.V35L+p.R83K+p.K103N+p.D121DH+p.K122E+p.D123E+p.I178IM+p.M184MV+p.G196E+p.T200A	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, E28EA, V35L, R83K, K103N, D121DH, K122E, D123E, I178IM, M184MV, G196E, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.I63IV+p.V90I+p.V118VI+p.K122E+p.I135IT+p.M184V+p.Q197E+p.T200TI+p.I202V+p.E204Q+p.Q207E+p.R211K+p.T215D	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, I63IV, V90I, V118VI, K122E, I135IT, M184V, Q197E, T200TI, I202V, E204Q, Q207E, R211K, T215D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.V35VI+p.L74I+p.L100I+p.K103N+p.K122E+p.I135IT+p.K173Q+p.D177E+p.I178IV+p.M184V+p.G196E+p.T200A+p.Q207NS+p.F214L+p.K219X+p.P225PH	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, V35VI, L74I, L100I, K103N, K122E, I135IT, K173Q, D177E, I178IV, M184V, G196E, T200A, Q207NS, F214L, K219X, P225PH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.I31L+p.K65R+p.K122E+p.Y181C+p.M184I+p.R211K+p.A288S+p.P294Q	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, I31L, K65R, K122E, Y181C, M184I, R211K, A288S, P294Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K103N+p.K122E+p.D123E+p.I135L+p.I142V+p.M184V+p.T200E+p.R211K+p.P225PH	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K103N, K122E, D123E, I135L, I142V, M184V, T200E, R211K, P225PH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K20R+p.V35T+p.T39A+p.D67N+p.T69D+p.K103N+p.K122E+p.D123DE+p.Q174QR+p.D177E+p.V179I+p.Y181C+p.M184V+p.T200TA+p.H208HY+p.R211T+p.F214L+p.T215L+p.D218E+p.K219Q+p.L228H	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K20R, V35T, T39A, D67N, T69D, K103N, K122E, D123DE, Q174QR, D177E, V179I, Y181C, M184V, T200TA, H208HY, R211T, F214L, T215L, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I2IT+p.K20KR+p.I31R+p.V35IL+p.A62V+p.V75I+p.K101Q+p.G141GE+p.S162X+p.T165TI+p.K173KR+p.Q174QR+p.M184V+p.G190S+p.T200TA+p.F214L+p.H221HY	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I2IT, K20KR, I31R, V35IL, A62V, V75I, K101Q, G141GE, S162X, T165TI, K173KR, Q174QR, M184V, G190S, T200TA, F214L, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31L+p.V35M+p.E40K+p.T58G+p.L74V+p.K122E+p.I178L+p.V179I+p.Y181C+p.M184V+p.Q207E+p.R211K+p.K219N+p.H221Y+p.L228R	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31L, V35M, E40K, T58G, L74V, K122E, I178L, V179I, Y181C, M184V, Q207E, R211K, K219N, H221Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.Y115YC+p.S162A+p.D177E+p.M184MV+p.Y188L+p.Q207E+p.R211K+p.P225PH	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, Y115YC, S162A, D177E, M184MV, Y188L, Q207E, R211K, P225PH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K104R+p.K122E+p.E138KR+p.I142T+p.D177E+p.I178M+p.V179I+p.M184V+p.G196E+p.T200K+p.Q207A+p.R211K+p.K219KE+p.I257L+p.A272P+p.K277R+p.A288S+p.I293V+p.E297R+p.L301M	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K104R, K122E, E138KR, I142T, D177E, I178M, V179I, M184V, G196E, T200K, Q207A, R211K, K219KE, I257L, A272P, K277R, A288S, I293V, E297R, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.K20KR+p.V35I+p.V60VI+p.R83K+p.D177DN+p.I178IM+p.G196E+p.Q207E	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, K20KR, V35I, V60VI, R83K, D177DN, I178IM, G196E, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11T+p.K20R+p.K22R+p.V60I+p.D123E+p.I135L+p.K166R+p.D177E+p.M184V+p.T200I+p.A272P+p.V276VI+p.I293V+p.E297A	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11T, K20R, K22R, V60I, D123E, I135L, K166R, D177E, M184V, T200I, A272P, V276VI, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.M184I+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	0.4	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, M184I, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.R125RG+p.L149LH+p.E169D+p.M184V+p.G196E+p.T200A+p.R211RK	Missense mutation	0.2	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, R125RG, L149LH, E169D, M184V, G196E, T200A, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K103N+p.D123E+p.I135IL+p.I180V+p.M184V+p.T200A+p.Q207E+p.D237DN+p.K238T+p.I244IV+p.V245M+p.A272P+p.K277R+p.T286A+p.E297R	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K103N, D123E, I135IL, I180V, M184V, T200A, Q207E, D237DN, K238T, I244IV, V245M, A272P, K277R, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K22R+p.V106I+p.I178L+p.R211K+p.F214FL+p.D250E+p.S251SN+p.K277R	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K22R, V106I, I178L, R211K, F214FL, D250E, S251SN, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K32KR+p.K103N+p.K122P+p.D123E+p.I142DV+p.K166KR+p.K173KE+p.D177DEG+p.M184V+p.I195IL+p.G196DE+p.F214FL+p.T215TI+p.K281KR	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K32KR, K103N, K122P, D123E, I142DV, K166KR, K173KE, D177DEG, M184V, I195IL, G196DE, F214FL, T215TI, K281KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35MT+p.V60I+p.K103N+p.K122E+p.A158S+p.S162A+p.M184MV+p.T200TA+p.I202IT+p.Q207E+p.R211RK+p.V245VE+p.T286A+p.I293IV	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35MT, V60I, K103N, K122E, A158S, S162A, M184MV, T200TA, I202IT, Q207E, R211RK, V245VE, T286A, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35VI+p.T69N+p.K70N+p.V90I+p.A98S+p.K101KE+p.K102KE+p.D121H+p.K122E+p.D177DE+p.I178L+p.Y181C+p.M184MIV+p.G190GA+p.G196GR+p.T200TA+p.H221HY+p.L228LR	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35VI, T69N, K70N, V90I, A98S, K101KE, K102KE, D121H, K122E, D177DE, I178L, Y181C, M184MIV, G190GA, G196GR, T200TA, H221HY, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V60I+p.S68G+p.K82R+p.N175NH+p.Q207QE+p.R211RK+p.K277KR+p.T286TA	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V60I, S68G, K82R, N175NH, Q207QE, R211RK, K277KR, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E169D+p.V179D+p.R211K+p.F214L+p.V245K+p.A272P+p.Q278Y+p.I293V	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E169D, V179D, R211K, F214L, V245K, A272P, Q278Y, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E28D+p.Y181YC+p.G196E+p.T200A+p.E204D+p.E297A	Missense mutation	0.4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E28D, Y181YC, G196E, T200A, E204D, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K64KR+p.L74V+p.K103N+p.V108I+p.Y181C+p.M184V+p.T200A+p.R211K+p.H221Y+p.L228H+p.V245L+p.A272P+p.K277R+p.T286A	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K64KR, L74V, K103N, V108I, Y181C, M184V, T200A, R211K, H221Y, L228H, V245L, A272P, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K65R+p.S68X+p.T69TI+p.K122E+p.I135V+p.M184V+p.T200TA	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K65R, S68X, T69TI, K122E, I135V, M184V, T200TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.L74LV+p.K103N+p.T165M+p.M184V+p.G196E+p.F214L+p.T286A+p.E297K	Missense mutation	0.4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, L74LV, K103N, T165M, M184V, G196E, F214L, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.V60I+p.S68SG+p.K102KQ+p.V111VIM+p.V118I+p.K122KE+p.D123DN+p.I135IT+p.A158S+p.T165I+p.Q174QR+p.Y181C+p.T200TA+p.E203K+p.Q207E+p.L210W+p.R211K+p.F214L+p.T215D+p.H221Y+p.L228H	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, V60I, S68SG, K102KQ, V111VIM, V118I, K122KE, D123DN, I135IT, A158S, T165I, Q174QR, Y181C, T200TA, E203K, Q207E, L210W, R211K, F214L, T215D, H221Y, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.R83K+p.V106VA+p.D123E+p.S162A	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, R83K, V106VA, D123E, S162A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.K65KR+p.L74LV+p.K103N+p.K122E+p.Y181C+p.T200A+p.R211K+p.F227C+p.M230L+p.V245Q+p.I293V+p.E297A	Missense mutation	0.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, K65KR, L74LV, K103N, K122E, Y181C, T200A, R211K, F227C, M230L, V245Q, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.T39K+p.T69A+p.K122E+p.E138G+p.I178L+p.Q207E	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, T39K, T69A, K122E, E138G, I178L, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.M41L+p.L74V+p.K103R+p.V179D+p.M184V+p.F214L	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39A, M41L, L74V, K103R, V179D, M184V, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35T+p.I50V+p.V60I+p.T69S+p.W88C+p.K122E+p.D123S+p.I135V+p.S162C+p.K173A+p.Q174K+p.D177E+p.T200A+p.Q207E+p.R211K+p.L228LF+p.V245Q+p.E248D+p.D250E+p.A272Q+p.K277R+p.T286A+p.E291D+p.I293V	Missense mutation	0.4	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35T, I50V, V60I, T69S, W88C, K122E, D123S, I135V, S162C, K173A, Q174K, D177E, T200A, Q207E, R211K, L228LF, V245Q, E248D, D250E, A272Q, K277R, T286A, E291D, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.L74LV+p.A98S+p.K103N+p.V108VI+p.K122P+p.D177E+p.M184V+p.K219X+p.H221HY+p.Y232H	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, L74LV, A98S, K103N, V108VI, K122P, D177E, M184V, K219X, H221HY, Y232H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22R+p.V35M+p.K70KE+p.K122E+p.I132IV+p.I178L+p.M184V+p.Y188L+p.T200A+p.Q207E	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22R, V35M, K70KE, K122E, I132IV, I178L, M184V, Y188L, T200A, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.L74LV+p.A98AS+p.K103KN+p.Y115YF+p.K122E+p.D123DQ+p.R172RK+p.K173N+p.I178IM+p.V179VI+p.M184V+p.T200I+p.P225PH	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, L74LV, A98AS, K103KN, Y115YF, K122E, D123DQ, R172RK, K173N, I178IM, V179VI, M184V, T200I, P225PH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KN+p.K70G+p.E138A+p.I142T+p.S162C+p.K173KE+p.I178IM+p.M184V+p.L210LF+p.R211K+p.F214L+p.V245T+p.V261VG+p.A272P+p.V292I	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KN, K70G, E138A, I142T, S162C, K173KE, I178IM, M184V, L210LF, R211K, F214L, V245T, V261VG, A272P, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K46KIM+p.K65R+p.V75L+p.V90VI+p.K102KR+p.K103KR+p.Y115F+p.D123E+p.E169D+p.D177E+p.V179VI+p.G190E+p.T200A+p.R211K	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K46KIM, K65R, V75L, V90VI, K102KR, K103KR, Y115F, D123E, E169D, D177E, V179VI, G190E, T200A, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.K65R+p.L100I+p.V108VI+p.D123E+p.I132L+p.I135T+p.T165I+p.I178L+p.Y181C+p.M184I+p.T200A+p.F214L+p.H221Y+p.P243T+p.A272P+p.K277R+p.E297K+p.V317A	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, K65R, L100I, V108VI, D123E, I132L, I135T, T165I, I178L, Y181C, M184I, T200A, F214L, H221Y, P243T, A272P, K277R, E297K, V317A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.K65R+p.V90VI+p.L100I+p.V108VI+p.D123E+p.I132L+p.I135T+p.T165I+p.I178L+p.Y181C+p.M184I+p.T200A+p.F214L+p.H221Y+p.T286TA	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, K65R, V90VI, L100I, V108VI, D123E, I132L, I135T, T165I, I178L, Y181C, M184I, T200A, F214L, H221Y, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.D67G+p.K70E+p.K103N+p.T128A+p.I135M+p.M184V+p.T200A+p.E204D+p.R211K+p.K219R+p.A272P+p.V276I+p.K281R+p.T286A+p.E297K	Missense mutation	0.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, D67G, K70E, K103N, T128A, I135M, M184V, T200A, E204D, R211K, K219R, A272P, V276I, K281R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.I50V+p.K103N+p.D110DG+p.K122E+p.I135T+p.T165I+p.D177DN+p.G196E+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, I50V, K103N, D110DG, K122E, I135T, T165I, D177DN, G196E, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.L74LI+p.K103N+p.V108I+p.K122E+p.K166KR+p.M184V+p.H221Y+p.P225H+p.K277R+p.T286A+p.E297R	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, L74LI, K103N, V108I, K122E, K166KR, M184V, H221Y, P225H, K277R, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.D67N+p.T69D+p.K122E+p.Q174K+p.I178IV+p.Y181C+p.G190A+p.L228LH	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, D67N, T69D, K122E, Q174K, I178IV, Y181C, G190A, L228LH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.S68G+p.K122E+p.I142V+p.D177E+p.M184V+p.T200I+p.R211K+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, S68G, K122E, I142V, D177E, M184V, T200I, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.S68G+p.L100I+p.K103N+p.D123E+p.T139KR+p.V179VI+p.M184V+p.K277R+p.T286A+p.E297K	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, S68G, L100I, K103N, D123E, T139KR, V179VI, M184V, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.V90I+p.L100I+p.K122E+p.D177E+p.I178M+p.Y188L+p.L210S+p.R211Q+p.K277R+p.V292I+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, V90I, L100I, K122E, D177E, I178M, Y188L, L210S, R211Q, K277R, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K66KR+p.L74V+p.K82KR+p.K102Q+p.Y115F+p.D123E+p.M184V+p.V245M+p.E248D+p.I257IL+p.A272P+p.K277R+p.I293V+p.K366R+p.T369A+p.A376T+p.K390R+p.A400T+p.T403A+p.E404D+p.K431KR+p.V435A+p.A437V+p.D460N+p.R461K+p.H483Y+p.L491S+p.S519N+p.A554NS+p.I556IV+p.K558R	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K66KR, L74V, K82KR, K102Q, Y115F, D123E, M184V, V245M, E248D, I257IL, A272P, K277R, I293V, K366R, T369A, A376T, K390R, A400T, T403A, E404D, K431KR, V435A, A437V, D460N, R461K, H483Y, L491S, S519N, A554NS, I556IV, K558R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70~+p.K102Q+p.S162C+p.K277R+p.I293V	Missense mutation	0.2	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70~, K102Q, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70E+p.M184V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70E, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74LI+p.K103N+p.D123E+p.T139TK+p.D177E+p.Y181C+p.M184V+p.T200A+p.Q207V+p.R211K+p.P294AS	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74LI, K103N, D123E, T139TK, D177E, Y181C, M184V, T200A, Q207V, R211K, P294AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16V+p.V35I+p.R83K+p.K122E+p.I135V+p.T139TA+p.T200A+p.Q207E+p.R211K+p.I244V+p.V245M+p.A272P+p.K281KR+p.L283I+p.E297EA	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16V, V35I, R83K, K122E, I135V, T139TA, T200A, Q207E, R211K, I244V, V245M, A272P, K281KR, L283I, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.S68G+p.K103N+p.T139A+p.M184V+p.T200I+p.I202V+p.R211K+p.T215S+p.K219E+p.L228H+p.W239I+p.V245E+p.A272P+p.P294T+p.E297Q	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, S68G, K103N, T139A, M184V, T200I, I202V, R211K, T215S, K219E, L228H, W239I, V245E, A272P, P294T, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.S68SG+p.D110DG+p.K122E+p.T200A+p.L210W+p.R211K+p.T215D	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, S68SG, D110DG, K122E, T200A, L210W, R211K, T215D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P14Q+p.I50V+p.T69D+p.K122E+p.M184V+p.Y188L+p.G196E+p.R211G+p.K238T+p.W239I	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P14Q, I50V, T69D, K122E, M184V, Y188L, G196E, R211G, K238T, W239I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P19S+p.K101P+p.K103N+p.E138G+p.E169K+p.K173E+p.D177E+p.I178M+p.V179I+p.G196E+p.V245E+p.A272P+p.E297K	Missense mutation	0.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P19S, K101P, K103N, E138G, E169K, K173E, D177E, I178M, V179I, G196E, V245E, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.V35VI+p.M41L+p.S68SG+p.T69T_SS+p.L74I+p.R83RK+p.V108I+p.T139M+p.S162D+p.S163T+p.K166R+p.D177E+p.V179I+p.Y181C+p.M184V+p.V189VI+p.I202V+p.L210W+p.R211K+p.T215H+p.A272P	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, V35VI, M41L, S68SG, T69T_SS, L74I, R83RK, V108I, T139M, S162D, S163T, K166R, D177E, V179I, Y181C, M184V, V189VI, I202V, L210W, R211K, T215H, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.E6D+p.V35VI+p.K103N+p.D123E+p.I142V+p.D177E+p.I178M+p.M184V+p.T200A+p.I244V+p.V245K+p.A272P+p.T286TA	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, E6D, V35VI, K103N, D123E, I142V, D177E, I178M, M184V, T200A, I244V, V245K, A272P, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K20R+p.V35L+p.S68G+p.K70G+p.K103NS+p.I135V+p.I142X+p.M184V+p.T200TA+p.E203EG+p.T215TA	Missense mutation	0.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K20R, V35L, S68G, K70G, K103NS, I135V, I142X, M184V, T200TA, E203EG, T215TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.T69TI+p.V106VI+p.I178IM+p.R211RK+p.V245X+p.T286TA	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, T69TI, V106VI, I178IM, R211RK, V245X, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.V35I+p.K43Q+p.E44D+p.D67N+p.T69D+p.A98G+p.V111I+p.D123I+p.E138G+p.V179F+p.Y181C+p.T200A+p.E203K+p.Q207D+p.H208Y+p.L210W+p.R211K+p.T215D+p.K223Q+p.V245E+p.A272T+p.Q278H+p.T286A+p.E297K	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, V35I, K43Q, E44D, D67N, T69D, A98G, V111I, D123I, E138G, V179F, Y181C, T200A, E203K, Q207D, H208Y, L210W, R211K, T215D, K223Q, V245E, A272T, Q278H, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.V35T+p.V60I+p.D67N+p.K70G+p.K102Q+p.V118I+p.D123E+p.E138A+p.S162C+p.K173KR+p.Q174K+p.M184V+p.G196E+p.Q207E+p.K219Q+p.D237E+p.V245M+p.S251N+p.K277R+p.I293IV+p.E297A+p.R358KT+p.D364DE	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, V35T, V60I, D67N, K70G, K102Q, V118I, D123E, E138A, S162C, K173KR, Q174K, M184V, G196E, Q207E, K219Q, D237E, V245M, S251N, K277R, I293IV, E297A, R358KT, D364DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9PS+p.K11KR+p.K20KR+p.A33AG+p.K65R+p.W88C+p.L100I+p.K103N+p.M184MV+p.E248D+p.K277R+p.T286A+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9PS, K11KR, K20KR, A33AG, K65R, W88C, L100I, K103N, M184MV, E248D, K277R, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9PS+p.V35L+p.V60I+p.K103KN+p.K122E+p.I132V+p.G196E+p.R211G	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9PS, V35L, V60I, K103KN, K122E, I132V, G196E, R211G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9Q+p.K20R+p.V35T+p.E36D+p.E40D+p.K49R+p.V90I+p.K122E+p.I135V+p.S162A+p.K173T+p.Q174K+p.D177E+p.I178M+p.M184V+p.V189I+p.T200A+p.Q207E+p.R211K+p.K219R	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9Q, K20R, V35T, E36D, E40D, K49R, V90I, K122E, I135V, S162A, K173T, Q174K, D177E, I178M, M184V, V189I, T200A, Q207E, R211K, K219R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K122Q+p.K166R+p.Q174X+p.D177E+p.Y181C+p.A272P+p.Q278QH+p.T286A	Missense mutation	0.4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K122Q, K166R, Q174X, D177E, Y181C, A272P, Q278QH, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.S162A+p.K166R+p.Y188L+p.T200E+p.Q207H+p.R211K	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, S162A, K166R, Y188L, T200E, Q207H, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48E+p.I135T+p.S162A+p.M184V+p.Q197H+p.T200A+p.R211G+p.E224ED	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48E, I135T, S162A, M184V, Q197H, T200A, R211G, E224ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48T+p.L74V+p.L100I+p.K103N+p.K122E+p.I135LV+p.T200A+p.T215C+p.L228LH+p.E248N+p.D250X+p.A272P	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48T, L74V, L100I, K103N, K122E, I135LV, T200A, T215C, L228LH, E248N, D250X, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.I159IV+p.T165TI+p.K166R+p.Q207E+p.R211K+p.E248D+p.A272P+p.I293V+p.E297Q	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, I159IV, T165TI, K166R, Q207E, R211K, E248D, A272P, I293V, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.A62V+p.K65R+p.I135T+p.Q174R+p.M184V+p.F214L	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, A62V, K65R, I135T, Q174R, M184V, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40EK+p.S48T+p.L74V+p.R83K+p.K101E+p.I135T+p.E138X+p.T139TS+p.S162C+p.T165TI+p.Y181C+p.M184V+p.G190A+p.I202V+p.R211K+p.V245EK	Missense mutation	0.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40EK, S48T, L74V, R83K, K101E, I135T, E138X, T139TS, S162C, T165TI, Y181C, M184V, G190A, I202V, R211K, V245EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39S+p.E40EV+p.M41L+p.S68G+p.V106VI+p.D123E+p.I142T+p.I178L+p.M184V+p.Y188L+p.G196E+p.T215D+p.L228H	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39S, E40EV, M41L, S68G, V106VI, D123E, I142T, I178L, M184V, Y188L, G196E, T215D, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.D123E+p.I142V+p.S162C+p.M184V+p.T200A+p.Q207E+p.R211RK+p.V245TI+p.A272S+p.K277R+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, D123E, I142V, S162C, M184V, T200A, Q207E, R211RK, V245TI, A272S, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.K70E+p.K102Q+p.D123E+p.I142IM+p.S162C+p.M184V+p.R211K+p.K277R+p.T286A+p.V292I+p.I293V+p.E297K+p.A304E+p.S322SA	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, K70E, K102Q, D123E, I142IM, S162C, M184V, R211K, K277R, T286A, V292I, I293V, E297K, A304E, S322SA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TI+p.K104H+p.V118I+p.D121DY+p.K122E+p.D123DN+p.I135IT+p.I142IV+p.S162X+p.K166KR+p.M184V+p.T200TA+p.F214L+p.V245E+p.K277R+p.I293V	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TI, K104H, V118I, D121DY, K122E, D123DN, I135IT, I142IV, S162X, K166KR, M184V, T200TA, F214L, V245E, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69D+p.K122E+p.D123E+p.I135M+p.S162CW+p.Y181YC+p.G196E+p.L210LS+p.R211K+p.K277KR	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69D, K122E, D123E, I135M, S162CW, Y181YC, G196E, L210LS, R211K, K277KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V10VI+p.V35M+p.T39A+p.E40D+p.K49E+p.E53D+p.S68N+p.K70G+p.K103N+p.D123E+p.I135T+p.I142K+p.V179I+p.M184V+p.G196E+p.A272P+p.L282LF+p.L283I+p.A288S+p.E291D+p.I293V+p.E297K	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V10VI, V35M, T39A, E40D, K49E, E53D, S68N, K70G, K103N, D123E, I135T, I142K, V179I, M184V, G196E, A272P, L282LF, L283I, A288S, E291D, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V118I+p.K122E+p.D123E+p.S163T+p.Q174E+p.Y181C+p.F214L+p.L228H+p.T240E	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V118I, K122E, D123E, S163T, Q174E, Y181C, F214L, L228H, T240E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.K65R+p.S68N+p.L80LF+p.K122E+p.S162AT+p.M184V+p.I202V+p.Q207E	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, K65R, S68N, L80LF, K122E, S162AT, M184V, I202V, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.M41L+p.K70T+p.V75I+p.Q85QL+p.W88S+p.V108I+p.D123E+p.D177E+p.I178L+p.M184V+p.G196E+p.V245E+p.D250E+p.A272P+p.K281R+p.A288S+p.P294S+p.E297K	Missense mutation	0.2	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, M41L, K70T, V75I, Q85QL, W88S, V108I, D123E, D177E, I178L, M184V, G196E, V245E, D250E, A272P, K281R, A288S, P294S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.V35T+p.E40ED+p.V60I+p.K73R+p.K122E+p.D123N+p.I135V+p.S162A+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207E+p.V245Q+p.E248N+p.K249Q+p.D250E+p.A272P+p.K281R+p.E291D+p.V292I+p.I293V	Missense mutation	0.3	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, V35T, E40ED, V60I, K73R, K122E, D123N, I135V, S162A, K173T, Q174K, D177E, I178M, T200A, Q207E, V245Q, E248N, K249Q, D250E, A272P, K281R, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.E28EG+p.V35VI+p.I47V+p.K49KR+p.V60I+p.T69D+p.K73M+p.V75A+p.V90VI+p.L100I+p.K103N+p.K122E+p.D123E+p.I135IT+p.I142L+p.P157A+p.S162SN+p.K173KR+p.D177EG+p.M184V+p.T200TA+p.Q207E+p.R211K+p.P225PH+p.L228HR+p.I244IV+p.V245VE	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, E28EG, V35VI, I47V, K49KR, V60I, T69D, K73M, V75A, V90VI, L100I, K103N, K122E, D123E, I135IT, I142L, P157A, S162SN, K173KR, D177EG, M184V, T200TA, Q207E, R211K, P225PH, L228HR, I244IV, V245VE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35A+p.A98S+p.K122E+p.M184V+p.G196E+p.R211K+p.E248N+p.K281R	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35A, A98S, K122E, M184V, G196E, R211K, E248N, K281R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35A+p.T39A+p.V60I+p.K65KR+p.K103KN+p.K122KE+p.Y181YC+p.M184MV+p.T200A+p.F214L+p.H221HY	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35A, T39A, V60I, K65KR, K103KN, K122KE, Y181YC, M184MV, T200A, F214L, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D123E+p.K166R+p.Q174E+p.D177E+p.I178M+p.R211K	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D123E, K166R, Q174E, D177E, I178M, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D67DN+p.R83RK+p.K102R+p.K103S+p.K104R+p.V106VI+p.K122E+p.E169X+p.K173E+p.Q174K+p.V179I+p.M184MV+p.G190A+p.T200TA+p.Q207QH+p.F214L+p.V245VA+p.K281KR+p.V292VI	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D67DN, R83RK, K102R, K103S, K104R, V106VI, K122E, E169X, K173E, Q174K, V179I, M184MV, G190A, T200TA, Q207QH, F214L, V245VA, K281KR, V292VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.E40D+p.V60VI+p.R83K+p.D123E+p.K173KEQ+p.D177E+p.I178IM+p.G196E+p.R211K+p.V245X+p.E248ED	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, E40D, V60VI, R83K, D123E, K173KEQ, D177E, I178IM, G196E, R211K, V245X, E248ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.I37IF+p.R83K+p.S162A+p.E169ED+p.T200TA+p.R211K+p.V245X+p.E248ED+p.Q278QH	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, I37IF, R83K, S162A, E169ED, T200TA, R211K, V245X, E248ED, Q278QH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K122E+p.Q145M+p.I202V+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K122E, Q145M, I202V, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K126KR+p.I135IL+p.I178M+p.G196E	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K126KR, I135IL, I178M, G196E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K173N+p.Y181C+p.R211S+p.L228R+p.A272P+p.K277R+p.I293V	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K173N, Y181C, R211S, L228R, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K65R+p.L100I+p.K103N+p.D121Y+p.K122E+p.I135T+p.S162C+p.Q174K+p.D177E+p.R211K+p.A272P+p.T286A+p.E297R	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K65R, L100I, K103N, D121Y, K122E, I135T, S162C, Q174K, D177E, R211K, A272P, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K70G+p.K122E+p.I135T+p.Q174K+p.M184V+p.R211K+p.V276VI+p.I293V	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K70G, K122E, I135T, Q174K, M184V, R211K, V276VI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.K103N+p.I135T+p.E138G+p.K166KR+p.T200TA+p.H208HY+p.R211K+p.I244V+p.D250E+p.A272P+p.I293V	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, K103N, I135T, E138G, K166KR, T200TA, H208HY, R211K, I244V, D250E, A272P, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S162C+p.P170T+p.I178L+p.I202V+p.R211K+p.K238R+p.V245K+p.A272S+p.K277R	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S162C, P170T, I178L, I202V, R211K, K238R, V245K, A272S, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S48T+p.K65R+p.L100I+p.K103N+p.K104KR+p.K122E+p.D177E+p.I178M+p.R211K+p.D250E+p.A272P+p.K277R+p.Q278QH+p.E297Q	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S48T, K65R, L100I, K103N, K104KR, K122E, D177E, I178M, R211K, D250E, A272P, K277R, Q278QH, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S68G+p.D123E+p.S134G+p.I135V+p.T139R+p.V179I+p.Y181C+p.T200A+p.I202V+p.E204EQ+p.L210LW+p.R211RK+p.T215D+p.K238T+p.A288AG	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S68G, D123E, S134G, I135V, T139R, V179I, Y181C, T200A, I202V, E204EQ, L210LW, R211RK, T215D, K238T, A288AG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S68SN+p.R83K+p.K101KR+p.D123E+p.S162SN+p.D177E+p.I178L+p.Q197E+p.T200TA+p.K201KR	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S68SN, R83K, K101KR, D123E, S162SN, D177E, I178L, Q197E, T200TA, K201KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.S48T+p.K70KT+p.V106I+p.T107S+p.K122P+p.V179D+p.M184V+p.Y188L+p.T200A+p.R211RK+p.D237DE+p.V292I+p.I293V	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, S48T, K70KT, V106I, T107S, K122P, V179D, M184V, Y188L, T200A, R211RK, D237DE, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39TA+p.L80LF+p.R83K+p.D121DY+p.K122KE+p.D123DE+p.M184MV+p.R211K+p.P243PS+p.I257IT+p.V292VI+p.E297Q	Missense mutation	0.4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39TA, L80LF, R83K, D121DY, K122KE, D123DE, M184MV, R211K, P243PS, I257IT, V292VI, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39TA+p.S68G+p.I135T+p.T165TI+p.K166KR+p.I178IM+p.T200TA+p.Q207QE+p.R211X+p.A272P+p.K277R+p.E297KR	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39TA, S68G, I135T, T165TI, K166KR, I178IM, T200TA, Q207QE, R211X, A272P, K277R, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39TA+p.V60I+p.V118I+p.K122E+p.I135R+p.T200A+p.R211K+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39TA, V60I, V118I, K122E, I135R, T200A, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T69I+p.R83K+p.K122E+p.D123S+p.I135V+p.S163T+p.E169D+p.D177E+p.I178M+p.V179I+p.M184V+p.R211K+p.V245K+p.E248D+p.A272P+p.K275R+p.R284K	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T69I, R83K, K122E, D123S, I135V, S163T, E169D, D177E, I178M, V179I, M184V, R211K, V245K, E248D, A272P, K275R, R284K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T69N+p.K70Q+p.R83K+p.K122KE+p.I135T+p.M184V+p.R211K	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T69N, K70Q, R83K, K122KE, I135T, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.S48E+p.K65R+p.E138EQ+p.A158S+p.Q174K+p.V179VI+p.Y181C+p.M184V+p.G190A+p.L210S+p.R211K+p.T215S+p.K219KN+p.M230L	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, S48E, K65R, E138EQ, A158S, Q174K, V179VI, Y181C, M184V, G190A, L210S, R211K, T215S, K219KN, M230L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K122E+p.K166KR+p.M184V+p.I202V+p.R211K	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K122E, K166KR, M184V, I202V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K70E+p.K102Q+p.K103N+p.D123E+p.S162C+p.K173E+p.Q174R+p.I178L+p.M184V+p.T200A+p.R211K+p.L228HR+p.M230L+p.L234I+p.V245KQ+p.K277R+p.L283I+p.V292I+p.I293V+p.Q334QR+p.G335X+p.T369TA+p.V381VI	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K70E, K102Q, K103N, D123E, S162C, K173E, Q174R, I178L, M184V, T200A, R211K, L228HR, M230L, L234I, V245KQ, K277R, L283I, V292I, I293V, Q334QR, G335X, T369TA, V381VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.S48E+p.K103N+p.E138EG+p.T200I+p.R211K+p.F214L	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, S48E, K103N, E138EG, T200I, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39A+p.M41L+p.K65R+p.V106A+p.K122E+p.T200I+p.L210W+p.R211K+p.T215S+p.F227L+p.V245K+p.D250E+p.A272P+p.K277R+p.V292I+p.P294ST+p.E297R	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39A, M41L, K65R, V106A, K122E, T200I, L210W, R211K, T215S, F227L, V245K, D250E, A272P, K277R, V292I, P294ST, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.D67G+p.T69D+p.K70GR+p.L100LI+p.K102KR+p.K103KN+p.V108VI+p.D121DY+p.K122KE+p.D123DE+p.I142IV+p.I178L+p.V179VIM+p.I180IV+p.M184MV	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, D67G, T69D, K70GR, L100LI, K102KR, K103KN, V108VI, D121DY, K122KE, D123DE, I142IV, I178L, V179VIM, I180IV, M184MV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.E36D+p.D123E+p.K126KR+p.I135V+p.S162A+p.K173T+p.Q174K+p.D177E+p.G196E+p.Q207E+p.R211K+p.V245Q+p.S251N+p.K281R+p.T286A+p.E291D+p.V292I+p.I293V+p.P294T	Missense mutation	0.4	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, E36D, D123E, K126KR, I135V, S162A, K173T, Q174K, D177E, G196E, Q207E, R211K, V245Q, S251N, K281R, T286A, E291D, V292I, I293V, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.I178L+p.M184V+p.S251I+p.K281R+p.L283I	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, I178L, M184V, S251I, K281R, L283I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.I37IM+p.K65R+p.S68K+p.K70KT+p.L74V+p.K103N+p.V108VI+p.K122KE+p.D123E+p.I135M+p.V179VI+p.Y181C+p.M184V+p.K219KR+p.H221HY	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, I37IM, K65R, S68K, K70KT, L74V, K103N, V108VI, K122KE, D123E, I135M, V179VI, Y181C, M184V, K219KR, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.K122Q+p.E169D+p.D177E+p.I178IM+p.Y181YC+p.R211RK+p.V245VE	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, K122Q, E169D, D177E, I178IM, Y181YC, R211RK, V245VE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39EK+p.S48T+p.V60VI+p.L74V+p.K103KN+p.D121H+p.K122E+p.I135T+p.K173T+p.Q174K+p.D177E+p.I178L+p.Y181C+p.M184V+p.T200A+p.Q207E+p.V245Q+p.A272P+p.E291D+p.V292I	Missense mutation	0.2	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39EK, S48T, V60VI, L74V, K103KN, D121H, K122E, I135T, K173T, Q174K, D177E, I178L, Y181C, M184V, T200A, Q207E, V245Q, A272P, E291D, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39TA+p.R83K+p.K103N+p.K122E+p.T165I+p.M184MV+p.H221HY+p.V245VE	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39TA, R83K, K103N, K122E, T165I, M184MV, H221HY, V245VE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K103N+p.K122E+p.D123S+p.I135V+p.E138G+p.K173I+p.Q174K+p.P176A+p.V179E+p.T200A+p.I202V+p.Q207D+p.R211K	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K103N, K122E, D123S, I135V, E138G, K173I, Q174K, P176A, V179E, T200A, I202V, Q207D, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K122E+p.D123N+p.M184V+p.I202V+p.L210F+p.R211K+p.V245I+p.E248D+p.D250E+p.A272P+p.I293V	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K122E, D123N, M184V, I202V, L210F, R211K, V245I, E248D, D250E, A272P, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.T69TN+p.K122E+p.D123DG+p.I135T+p.K173S+p.Q174K+p.D177E+p.K201KT+p.Q207A+p.R211S+p.T286TA+p.P294AT	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, T69TN, K122E, D123DG, I135T, K173S, Q174K, D177E, K201KT, Q207A, R211S, T286TA, P294AT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.M41L+p.L74LV+p.K122E+p.I142AT+p.S162SN+p.K166KR+p.M184V+p.G196E+p.L210W+p.R211K+p.T215DHY	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, M41L, L74LV, K122E, I142AT, S162SN, K166KR, M184V, G196E, L210W, R211K, T215DHY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.R83K+p.K102Q+p.V106M+p.K122E+p.I135V+p.S162C+p.T165TI+p.Q174R+p.D177E+p.V179D+p.G196E+p.I202V+p.R211Q+p.F214L+p.A272P+p.K277R+p.L283LI+p.I293V+p.M357ML+p.I375IV	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, R83K, K102Q, V106M, K122E, I135V, S162C, T165TI, Q174R, D177E, V179D, G196E, I202V, R211Q, F214L, A272P, K277R, L283LI, I293V, M357ML, I375IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.R83K+p.K122KE+p.D123E+p.I135T+p.P176PS+p.M184V+p.T200A+p.V245E+p.L283LF+p.A288S+p.V292I+p.I293V	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, R83K, K122KE, D123E, I135T, P176PS, M184V, T200A, V245E, L283LF, A288S, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.R83K+p.V106M+p.K122E+p.I135V+p.T165TI+p.Q174R+p.D177E+p.V179D+p.G196E+p.I202V+p.R211Q+p.F214L+p.A272P+p.K277R+p.L283LI+p.I293V	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, R83K, V106M, K122E, I135V, T165TI, Q174R, D177E, V179D, G196E, I202V, R211Q, F214L, A272P, K277R, L283LI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.V106M+p.D123E+p.I135T+p.V179D+p.M184V+p.F214L+p.Y232H+p.I244V+p.T286A+p.I293V	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, V106M, D123E, I135T, V179D, M184V, F214L, Y232H, I244V, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.V60I+p.K102R+p.K103N+p.K122E+p.D123DN+p.I132V+p.I135IT+p.M184V+p.G196E+p.R211G+p.P225H	Missense mutation	0.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, V60I, K102R, K103N, K122E, D123DN, I132V, I135IT, M184V, G196E, R211G, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.V60VI+p.K103N+p.D121C+p.K122E+p.I142T+p.D177E+p.M184V+p.G196E+p.R211RK+p.F214L+p.K219KQ+p.P225H+p.V245VM	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, V60VI, K103N, D121C, K122E, I142T, D177E, M184V, G196E, R211RK, F214L, K219KQ, P225H, V245VM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.F171FY+p.T200I+p.I202V+p.R211K	Missense mutation	0.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, F171FY, T200I, I202V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V75X+p.K122Q+p.S162SC+p.K173KN+p.Q174QK+p.I178LM+p.I180V+p.M184MV+p.R211K+p.F214L+p.L228H	Missense mutation	0.4	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V75X, K122Q, S162SC, K173KN, Q174QK, I178LM, I180V, M184MV, R211K, F214L, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K20KR+p.R83K+p.K122E+p.S162C+p.I195IT+p.R211G+p.N255NH+p.A272P+p.V292VI+p.I293V	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K20KR, R83K, K122E, S162C, I195IT, R211G, N255NH, A272P, V292VI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K32KR+p.K65R+p.V90I+p.L100I+p.K103N+p.K122E+p.D123N+p.E169D+p.T200A+p.Q207E+p.R211K+p.F214L+p.K277R+p.Q278H	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K32KR, K65R, V90I, L100I, K103N, K122E, D123N, E169D, T200A, Q207E, R211K, F214L, K277R, Q278H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.V35I+p.K122KE+p.I202IV+p.R211RK+p.A288AS	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, V35I, K122KE, I202IV, R211RK, A288AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V90I+p.K122E+p.M184V+p.T200A	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V90I, K122E, M184V, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101E+p.K102Q+p.S162C+p.K277R+p.I293V	Missense mutation	0.4	Susceptible	[31]
p.K102Q+p.E138K+p.S162C+p.K277R+p.I293V	Missense mutation	1.8	Susceptible	[31]
p.K101E+p.K102Q+p.E138K+p.S162C+p.M184I+p.K277R+p.I293V	Missense mutation	0.5	Susceptible	[31]
p.K102Q+p.E138K+p.S162C+p.M184I+p.K277R+p.I293V	Missense mutation	0.8	Susceptible	[31]
p.K101E+p.K102Q+p.E138K+p.S162C+p.K277R+p.I293V	Missense mutation	1.2	Susceptible	[31]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

In Vitro Model

HEK293T cells

Kidney

Homo sapiens (Human)

CVCL_0063

Experiment for
Molecule Alteration

Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay

Mechanism Description

The K101E substitution conferred large reductions in susceptibility to NVP and DLV, while more modest reductions in susceptibility to EFV, ETR, or RPV were observed. In contrast, the E138k substitution did not confer large reductions in susceptibility to any of the NNRTIs and only small reductions in susceptibility to ETR and RPV. That is because that the K101E substitution compensates for the impaired viral replication capacity of HIV-1 containing M184I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A33AV+p.K46KQ+p.V60I+p.A62AV+p.D67DG+p.S68G+p.K70KR+p.K122E+p.D123DN+p.I135T+p.E138EA+p.I142IM+p.T165I+p.I178IL+p.M184MV+p.G190GA+p.I195L+p.G196E+p.Q207A+p.R211K+p.T215X+p.K219KQ	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A33AV, K46KQ, V60I, A62AV, D67DG, S68G, K70KR, K122E, D123DN, I135T, E138EA, I142IM, T165I, I178IL, M184MV, G190GA, I195L, G196E, Q207A, R211K, T215X, K219KQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A33AV+p.V35I+p.V60I+p.D67N+p.K70R+p.L74I+p.K101KR+p.K102KR+p.Y115F+p.D121H+p.K122E+p.D177E+p.I180V+p.M184V+p.G190A+p.T200A+p.D218E+p.K219Q+p.L228H+p.I244V+p.A272P+p.E291D	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A33AV, V35I, V60I, D67N, K70R, L74I, K101KR, K102KR, Y115F, D121H, K122E, D177E, I180V, M184V, G190A, T200A, D218E, K219Q, L228H, I244V, A272P, E291D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.D67DG+p.T69DN+p.K70KN+p.V75T+p.K82KR+p.K101R+p.K103N+p.V118VI+p.K122E+p.R125RG+p.I135T+p.M184V+p.T200A+p.Q207E+p.V245Q+p.L246I	Missense mutation	0.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, D67DG, T69DN, K70KN, V75T, K82KR, K101R, K103N, V118VI, K122E, R125RG, I135T, M184V, T200A, Q207E, V245Q, L246I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.V75T+p.D86E+p.V90VI+p.K101P+p.K103N+p.K122P+p.I135T+p.D177E+p.D192N+p.T200TA+p.F214L	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, V75T, D86E, V90VI, K101P, K103N, K122P, I135T, D177E, D192N, T200TA, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98AS+p.V106VA+p.I135V+p.Y181YC+p.V189VI+p.T200TA	Missense mutation	1.1	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98AS, V106VA, I135V, Y181YC, V189VI, T200TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.D121Y+p.K122E+p.I135L+p.E138A+p.V179I+p.M184MV+p.Q207E+p.R211K+p.P243S+p.V245E+p.E297A	Missense mutation	1	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, D121Y, K122E, I135L, E138A, V179I, M184MV, Q207E, R211K, P243S, V245E, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K102E+p.S162H+p.Q207E+p.R211K+p.A272P+p.E297K	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K102E, S162H, Q207E, R211K, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K103KN+p.K122E+p.D123N+p.I135T+p.I142V+p.M184V+p.T200A+p.T215F	Missense mutation	2.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K103KN, K122E, D123N, I135T, I142V, M184V, T200A, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K103N+p.D121H+p.K122E+p.N136NH+p.N137NI+p.L149LH+p.S156S*+p.Q174R+p.D177E+p.V179I+p.V245M	Missense mutation	0.8	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K103N, D121H, K122E, N136NH, N137NI, L149LH, S156S*, Q174R, D177E, V179I, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.V106A+p.I135T+p.Q197P+p.R211G+p.F214L+p.A272P+p.T286A+p.E297R	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, V106A, I135T, Q197P, R211G, F214L, A272P, T286A, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CF+p.D67N+p.K70R+p.L74V+p.Y115F+p.E138E*+p.S162C+p.I178L+p.V179I+p.Y181C+p.M184V+p.E194D+p.I195IMT+p.T200A+p.E203K+p.Q207E+p.K219E+p.V245E	Missense mutation	0.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CF, D67N, K70R, L74V, Y115F, E138E*, S162C, I178L, V179I, Y181C, M184V, E194D, I195IMT, T200A, E203K, Q207E, K219E, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.C38CF+p.T39TS+p.K122E+p.I135IT+p.W153WR+p.K154KE+p.G196R	Missense mutation	1	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included C38CF, T39TS, K122E, I135IT, W153WR, K154KE, G196R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D121H+p.K122E+p.I135L+p.E138G+p.I178IL+p.V179VI+p.G190GA	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D121H, K122E, I135L, E138G, I178IL, V179VI, G190GA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D121Y+p.K122E+p.D177E+p.A272P+p.K281R+p.L283I+p.A288T+p.T296S	Missense mutation	1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D121Y, K122E, D177E, A272P, K281R, L283I, A288T, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123DE+p.D177DE+p.R211K+p.K277KR+p.K281KR+p.I293IVM+p.P294PS+p.E297EG	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123DE, D177DE, R211K, K277KR, K281KR, I293IVM, P294PS, E297EG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123DE+p.I135T+p.G196E+p.T200A+p.T286TA+p.E297X	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123DE, I135T, G196E, T200A, T286TA, E297X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.E138EK+p.S162C+p.I178L+p.Q207E+p.R211K+p.A272P+p.K277R+p.T286A+p.A288T+p.E297K	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, E138EK, S162C, I178L, Q207E, R211K, A272P, K277R, T286A, A288T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.E138G+p.S162C+p.P176S+p.D177E+p.I178L+p.T200A	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, E138G, S162C, P176S, D177E, I178L, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I135V+p.D177E+p.T200S+p.R211K+p.V245VM+p.D250DE	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I135V, D177E, T200S, R211K, V245VM, D250DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I178IM+p.V179VI+p.I202V+p.F214FL	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I178IM, V179VI, I202V, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I178M+p.R211K	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I178M, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.Q174K+p.I178M+p.V179VE+p.G196E+p.T200A+p.Q207X+p.R211A	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, Q174K, I178M, V179VE, G196E, T200A, Q207X, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.Q207E	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123N+p.I142V+p.T165TI+p.D177E+p.R211K+p.V245K+p.A272P+p.K277R+p.L283I+p.I293V+p.E297A	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123N, I142V, T165TI, D177E, R211K, V245K, A272P, K277R, L283I, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D177E+p.Q207E	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D177E, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70GR+p.L74LV+p.K103R+p.K122E+p.I159IL+p.T165I+p.Y181C+p.G190A+p.T200I+p.F214L+p.T215I+p.K219E+p.V245E	Missense mutation	1	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70GR, L74LV, K103R, K122E, I159IL, T165I, Y181C, G190A, T200I, F214L, T215I, K219E, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70KR+p.K101KEQ+p.E169D+p.Y181C+p.M184V+p.G190A+p.Q207E+p.R211K+p.T215F+p.K219Q	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70KR, K101KEQ, E169D, Y181C, M184V, G190A, Q207E, R211K, T215F, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K122E+p.M184V+p.T200TI+p.R211K+p.T215I+p.K219E	Missense mutation	1	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K122E, M184V, T200TI, R211K, T215I, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K173X+p.D177DN+p.M184V+p.G196E+p.Q197H+p.T200A+p.Q207E+p.T215X+p.K219Q	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K173X, D177DN, M184V, G196E, Q197H, T200A, Q207E, T215X, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.S162X+p.D177DE+p.G196E+p.Q207H+p.R211K+p.Q269QH+p.K277R+p.I293V	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, S162X, D177DE, G196E, Q207H, R211K, Q269QH, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.S68N+p.L74V+p.R83RK+p.K101H+p.I135V+p.Y181C+p.G190A+p.R211K+p.T215Y+p.K219E	Missense mutation	0.7	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, S68N, L74V, R83RK, K101H, I135V, Y181C, G190A, R211K, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69N+p.K101H+p.K103KR+p.I142V+p.E169D+p.M184V+p.K219Q+p.L228X	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69N, K101H, K103KR, I142V, E169D, M184V, K219Q, L228X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28A+p.A62V+p.K65R+p.S68K+p.K103N+p.K122E+p.D123N+p.I135L+p.S162A+p.M184V+p.Y188L+p.G196E+p.Q207E+p.R211K+p.F214L+p.K219T	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28A, A62V, K65R, S68K, K103N, K122E, D123N, I135L, S162A, M184V, Y188L, G196E, Q207E, R211K, F214L, K219T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28A+p.K64H+p.D67N+p.T69N+p.K70R+p.L74V+p.K103KN+p.D121Y+p.K122E+p.Y181C+p.M184V+p.G190GA+p.T200TA+p.R211K+p.T215F+p.D218E+p.K219Q+p.V245EK+p.A272P+p.L283I	Missense mutation	3	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28A, K64H, D67N, T69N, K70R, L74V, K103KN, D121Y, K122E, Y181C, M184V, G190GA, T200TA, R211K, T215F, D218E, K219Q, V245EK, A272P, L283I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EG+p.A62AV+p.K101KE+p.K102KE+p.K103KN+p.M184V+p.G190A+p.I195K+p.G196E+p.H221HY+p.E248N+p.T286A	Missense mutation	0.7	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EG, A62AV, K101KE, K102KE, K103KN, M184V, G190A, I195K, G196E, H221HY, E248N, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28EG+p.R83RK+p.V90VI+p.A98AS+p.K122E+p.Q197P+p.R211RK	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28EG, R83RK, V90VI, A98AS, K122E, Q197P, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28G+p.V35L+p.D67N+p.K70R+p.M184V+p.G196E+p.K219E	Missense mutation	2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28G, V35L, D67N, K70R, M184V, G196E, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28R+p.K32E+p.K122KE+p.I135V+p.S162C+p.M184V+p.T200A	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28R, K32E, K122KE, I135V, S162C, M184V, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28X+p.T39A+p.M41L+p.E44D+p.D67N+p.T69D+p.L74V+p.K101E+p.V108VI+p.V118I+p.D123E+p.I135T+p.I178IM+p.Y181C+p.M184V+p.G190A+p.T200A+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228N+p.P294Q	Missense mutation	2.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28X, T39A, M41L, E44D, D67N, T69D, L74V, K101E, V108VI, V118I, D123E, I135T, I178IM, Y181C, M184V, G190A, T200A, Q207E, L210W, R211K, T215Y, L228N, P294Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E36D+p.K49R+p.V60I+p.I135V+p.Q145M+p.Q174H+p.G196E+p.Q207E+p.R211K	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E36D, K49R, V60I, I135V, Q145M, Q174H, G196E, Q207E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E36ED+p.A98AG+p.K101KE+p.V179VA+p.V189VI+p.T200K+p.R211K+p.F214FL	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E36ED, A98AG, K101KE, V179VA, V189VI, T200K, R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E36ED+p.K49R+p.K103NS+p.E169EK+p.I178M+p.Q207E	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E36ED, K49R, K103NS, E169EK, I178M, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40D+p.K49R+p.V60I+p.D67N+p.T69D+p.K70R+p.L74V+p.L100I+p.K103N+p.D121HL+p.K122E+p.D123E+p.F130FI+p.N137NI+p.S162N+p.T165I+p.E169D+p.D177E+p.I178IM+p.M184V+p.T200X+p.I202V+p.E204K+p.Q207E+p.R211K+p.F214L+p.K219Q+p.L228H+p.V245Q	Missense mutation	0.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40D, K49R, V60I, D67N, T69D, K70R, L74V, L100I, K103N, D121HL, K122E, D123E, F130FI, N137NI, S162N, T165I, E169D, D177E, I178IM, M184V, T200X, I202V, E204K, Q207E, R211K, F214L, K219Q, L228H, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40EK+p.M41L+p.A62V+p.V75I+p.V90VA+p.K103N+p.K122E+p.I135T+p.I142V+p.W153X+p.P157PS+p.S162A+p.D177DE+p.Y181C+p.T200A+p.L210F+p.T215Y+p.P226PS+p.L228R+p.V245VE	Missense mutation	2.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40EK, M41L, A62V, V75I, V90VA, K103N, K122E, I135T, I142V, W153X, P157PS, S162A, D177DE, Y181C, T200A, L210F, T215Y, P226PS, L228R, V245VE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40F+p.M41L+p.K43E+p.K64H+p.D67N+p.T69N+p.L74V+p.V90I+p.K101H+p.V106I+p.V118I+p.K122E+p.I135T+p.I142V+p.V179F+p.Y181C+p.M184V+p.G190A+p.Q197P+p.T200E+p.H208Y+p.L210W+p.R211K+p.T215F+p.D218E+p.K219E+p.E224D+p.L228R+p.A288AT	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40F, M41L, K43E, K64H, D67N, T69N, L74V, V90I, K101H, V106I, V118I, K122E, I135T, I142V, V179F, Y181C, M184V, G190A, Q197P, T200E, H208Y, L210W, R211K, T215F, D218E, K219E, E224D, L228R, A288AT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E42EK+p.K103KN+p.K104KR+p.D123E+p.K173KR+p.D177E+p.E194ED+p.R211RK+p.K219KE	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E42EK, K103KN, K104KR, D123E, K173KR, D177E, E194ED, R211RK, K219KE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E53EK+p.K122A+p.I135V+p.Q174E+p.T200K+p.R211Q+p.F214L+p.K219KQ	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E53EK, K122A, I135V, Q174E, T200K, R211Q, F214L, K219KQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6AD+p.T7TA+p.V60I+p.D67N+p.T69TN+p.K70R+p.I94IV+p.K103N+p.K122E+p.I135T+p.M184V+p.G196E+p.T200TA+p.L210F+p.R211RK+p.F214L+p.K219Q+p.K275KR	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6AD, T7TA, V60I, D67N, T69TN, K70R, I94IV, K103N, K122E, I135T, M184V, G196E, T200TA, L210F, R211RK, F214L, K219Q, K275KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.D123E+p.I142V+p.S162C+p.D177E+p.T200I+p.V245VM+p.A272P+p.V276I+p.K277R+p.T286A+p.A288T+p.I293V	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, D123E, I142V, S162C, D177E, T200I, V245VM, A272P, V276I, K277R, T286A, A288T, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K103N+p.T200I+p.R211K+p.V245Q+p.T286TP+p.I293V	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K103N, T200I, R211K, V245Q, T286TP, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K11KR+p.V60VI+p.K101R+p.E169D+p.T200TI+p.Q207X+p.R211RK+p.A272P+p.V292VI+p.E297AT	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K11KR, V60VI, K101R, E169D, T200TI, Q207X, R211RK, A272P, V292VI, E297AT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K82R+p.R83K+p.K103N+p.I135IT+p.S162Y+p.E169D+p.Q207QE	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K82R, R83K, K103N, I135IT, S162Y, E169D, Q207QE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.D67DN+p.K103KN+p.I135IV+p.Y144YH+p.V179VI+p.M184MV+p.T200TA+p.E203EK+p.Q207E+p.L210LW+p.T215DY+p.I293VA+p.E297EA	Missense mutation	3.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, D67DN, K103KN, I135IV, Y144YH, V179VI, M184MV, T200TA, E203EK, Q207E, L210LW, T215DY, I293VA, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V21I+p.E29EG+p.V60I+p.I142T+p.G196R+p.T200I+p.R211K+p.T215F+p.V245K+p.E248D+p.D250E+p.A272P+p.V276VA+p.E291EG+p.E297K	Missense mutation	2.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V21I, E29EG, V60I, I142T, G196R, T200I, R211K, T215F, V245K, E248D, D250E, A272P, V276VA, E291EG, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V35I+p.D67N+p.K70R+p.K102E+p.D123E+p.D177E+p.I178IM+p.M184V+p.Q207E+p.T215Y+p.K219E	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V35I, D67N, K70R, K102E, D123E, D177E, I178IM, M184V, Q207E, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V35I+p.M41L+p.K43E+p.L74V+p.R83K+p.V118I+p.D123E+p.K166KQ+p.D177E+p.I178L+p.M184V+p.I202V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K249KR	Missense mutation	2.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V35I, M41L, K43E, L74V, R83K, V118I, D123E, K166KQ, D177E, I178L, M184V, I202V, Q207E, L210W, R211K, T215Y, K249KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V8VI+p.E40ED+p.R83K+p.D123E+p.A158S+p.T165I+p.I195IV+p.R211K	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V8VI, E40ED, R83K, D123E, A158S, T165I, I195IV, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.I135L+p.I178L+p.R211K+p.A288S+p.E297K	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, I135L, I178L, R211K, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.K11KR+p.T27TS+p.E40ED+p.R83K+p.K173KR	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, K11KR, T27TS, E40ED, R83K, K173KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.K122E+p.I135T+p.E138A+p.I142IV+p.T200I+p.R211RK+p.F214L	Missense mutation	1.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, K122E, I135T, E138A, I142IV, T200I, R211RK, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.V35T+p.K49R+p.V60VI+p.R83RK+p.D123E+p.T200TA+p.Q207QE+p.R211RK+p.D250E+p.A272AS+p.K277KR+p.K281KR+p.T286TA+p.E297K	Missense mutation	0.8	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, V35T, K49R, V60VI, R83RK, D123E, T200TA, Q207QE, R211RK, D250E, A272AS, K277KR, K281KR, T286TA, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.Y56YH+p.K101KR+p.I135T+p.D177E+p.Q207E+p.R211K+p.V245M+p.E248ED+p.D250X+p.A272P+p.K277KR+p.I293V+p.T296S+p.E297K	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, Y56YH, K101KR, I135T, D177E, Q207E, R211K, V245M, E248ED, D250X, A272P, K277KR, I293V, T296S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EG+p.K20R+p.K103R+p.E169ED+p.R211Q+p.F214L	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EG, K20R, K103R, E169ED, R211Q, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.K20KR+p.V35VA+p.K49KR+p.D67N+p.T69TN+p.K70R+p.W88C+p.K101KE+p.K103KN+p.V118VI+p.T139TI+p.A158S+p.S162D+p.K166R+p.E169D+p.M184V+p.G190GA+p.R211K+p.F214L+p.T215F+p.K219E+p.V245M+p.E297K	Missense mutation	3.2	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, K20KR, V35VA, K49KR, D67N, T69TN, K70R, W88C, K101KE, K103KN, V118VI, T139TI, A158S, S162D, K166R, E169D, M184V, G190GA, R211K, F214L, T215F, K219E, V245M, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.V35E+p.T39A+p.S68SN+p.V90I+p.K104N+p.K122E+p.I135R+p.D177E+p.Y181C+p.I202V+p.F214FL+p.H221Y	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, V35E, T39A, S68SN, V90I, K104N, K122E, I135R, D177E, Y181C, I202V, F214FL, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.D67N+p.S68G+p.T69N+p.K101Q+p.K122E+p.D123DN+p.I135T+p.V179F+p.Y181C+p.G190A+p.E204EK+p.Q207QH+p.R211RK+p.T215L+p.K219Q+p.H221Y+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, D67N, S68G, T69N, K101Q, K122E, D123DN, I135T, V179F, Y181C, G190A, E204EK, Q207QH, R211RK, T215L, K219Q, H221Y, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.G18GD+p.E28EG+p.K122E+p.I142T+p.G196E+p.F214FL+p.V245E+p.A272P+p.K277R+p.A288S	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, G18GD, E28EG, K122E, I142T, G196E, F214FL, V245E, A272P, K277R, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K101E+p.K122E+p.I142T+p.G196E+p.F214L+p.V245E+p.L260LF+p.A272P+p.K277R+p.A288S+p.V292I	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K101E, K122E, I142T, G196E, F214L, V245E, L260LF, A272P, K277R, A288S, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.K20R+p.V35M+p.T39A+p.M41L+p.G45E+p.D67N+p.T69D+p.V118I+p.K122E+p.D123E+p.M184V+p.L210W+p.R211K+p.T215Y+p.L228F+p.A272P+p.L282K+p.E297K	Missense mutation	2.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, K20R, V35M, T39A, M41L, G45E, D67N, T69D, V118I, K122E, D123E, M184V, L210W, R211K, T215Y, L228F, A272P, L282K, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.V10VA+p.K11R+p.K122E+p.G196R+p.T216TI+p.V245VM+p.E248N+p.K249Q+p.V276VI+p.T286A	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, V10VA, K11R, K122E, G196R, T216TI, V245VM, E248N, K249Q, V276VI, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6K+p.V35M+p.T39AV+p.M41ML+p.V60VI+p.D67N+p.S68SG+p.K122E+p.R172K+p.Q174R+p.Y188L+p.G196E+p.R199RG+p.Q207E+p.L210W+p.T215C+p.K219E+p.H235HR+p.T286A+p.E297K	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6K, V35M, T39AV, M41ML, V60VI, D67N, S68SG, K122E, R172K, Q174R, Y188L, G196E, R199RG, Q207E, L210W, T215C, K219E, H235HR, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G45GE+p.D123EG+p.I135V+p.E169EG+p.I178L+p.G196E+p.R211RG+p.F214L	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included G45GE, D123EG, I135V, E169EG, I178L, G196E, R211RG, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135T+p.I202V+p.Q207QK+p.R211RK+p.V245M+p.K277KR+p.T286A+p.A288S+p.I293V+p.E297K	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135T, I202V, Q207QK, R211RK, V245M, K277KR, T286A, A288S, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135V+p.K154KE+p.K173E+p.R211S+p.V245E+p.D250E+p.S251C+p.P294A	Missense mutation	2.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135V, K154KE, K173E, R211S, V245E, D250E, S251C, P294A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135V+p.R211NS+p.V292VI	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135V, R211NS, V292VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I47IF+p.E53EK+p.S105SL+p.D123X+p.P176PS+p.V189I+p.S191ST+p.Q207E+p.R211K+p.V241I+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I47IF, E53EK, S105SL, D123X, P176PS, V189I, S191ST, Q207E, R211K, V241I, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I50IV+p.I63IL+p.K65R+p.S68X+p.A98S+p.K103N+p.I142IS+p.D177N+p.Y181C+p.Q197P+p.T200TA+p.H221Y+p.V245L+p.Q278N+p.T286A+p.I293V+p.E297K	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I50IV, I63IL, K65R, S68X, A98S, K103N, I142IS, D177N, Y181C, Q197P, T200TA, H221Y, V245L, Q278N, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I50IV+p.K102KQ+p.K166KR+p.K173X+p.Q174K+p.D177DE+p.I178L+p.G196GE+p.Q197K+p.Q207E+p.R211K+p.V245EQ+p.D250E+p.A272P+p.K277R	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I50IV, K102KQ, K166KR, K173X, Q174K, D177DE, I178L, G196GE, Q197K, Q207E, R211K, V245EQ, D250E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5V+p.E6K+p.K20R+p.M41L+p.E44D+p.V60I+p.D67N+p.T69D+p.E89K+p.K102Q+p.K103N+p.V118I+p.K122E+p.E138K+p.I142V+p.V179I+p.Y188L+p.T200A+p.Q207N+p.R211K+p.F214L	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5V, E6K, K20R, M41L, E44D, V60I, D67N, T69D, E89K, K102Q, K103N, V118I, K122E, E138K, I142V, V179I, Y188L, T200A, Q207N, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I5V+p.R83K+p.S163T+p.T165TI+p.E169ED+p.T200A+p.Q207E	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I5V, R83K, S163T, T165TI, E169ED, T200A, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101KQ+p.K103KR+p.V179VI+p.I202IV+p.R211RK+p.F214L+p.A272S+p.L282LF+p.T286TA	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K101KQ, K103KR, V179VI, I202IV, R211RK, F214L, A272S, L282LF, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101KQ+p.K103N+p.I132IT+p.I135X+p.D177DG+p.M184V+p.Q197E+p.I202IV+p.Q207H+p.R211K+p.P225H	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K101KQ, K103N, I132IT, I135X, D177DG, M184V, Q197E, I202IV, Q207H, R211K, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101KR+p.I135L+p.S162C+p.Q207E+p.T215L+p.V245M	Missense mutation	0.9	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K101KR, I135L, S162C, Q207E, T215L, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102KQ+p.K103N+p.D177E+p.T200A+p.R211T+p.A272S+p.K281KR+p.T286A	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102KQ, K103N, D177E, T200A, R211T, A272S, K281KR, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102KR+p.K103N+p.D123E+p.I135T+p.E138A+p.I142T+p.K173Q+p.R211K+p.P225PH	Missense mutation	1.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102KR, K103N, D123E, I135T, E138A, I142T, K173Q, R211K, P225PH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.Q145M+p.S162C	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, Q145M, S162C.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.Q151M+p.S162C+p.K277R+p.I293V	Missense mutation	2.9	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, Q151M, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.S162C+p.K277R+p.I293V	Missense mutation	1	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103KN+p.K122KE+p.I142IV+p.D177DE+p.G213GR	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103KN, K122KE, I142IV, D177DE, G213GR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103KN+p.V106I+p.I135V+p.Q174N+p.F214L	Missense mutation	1.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103KN, V106I, I135V, Q174N, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.D121DY+p.K122KE+p.D123DE+p.I135T+p.K166KR+p.D177DE+p.I178ILM+p.T200A+p.R211K+p.F214L+p.A288AT	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, D121DY, K122KE, D123DE, I135T, K166KR, D177DE, I178ILM, T200A, R211K, F214L, A288AT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.D123E+p.D177E+p.Y181YC+p.T200I+p.I202V+p.R211K+p.A272P+p.Q278E+p.L283I+p.E297EG+p.E298A	Missense mutation	0.9	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, D123E, D177E, Y181YC, T200I, I202V, R211K, A272P, Q278E, L283I, E297EG, E298A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.I135ILM+p.M184V+p.Q207X+p.R211K+p.K223KT+p.V245M+p.A272P+p.L283LI	Missense mutation	0.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, I135ILM, M184V, Q207X, R211K, K223KT, V245M, A272P, L283LI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.K122E+p.I135T+p.Y188F+p.I202V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, K122E, I135T, Y188F, I202V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.R211K+p.V292I+p.I293V	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, R211K, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.K20R+p.V60VIM+p.S68SG+p.V90L+p.I135L+p.S162DN+p.K166KR+p.N175NH+p.I178L+p.V189I+p.G190GS+p.T200TA+p.R211RK+p.P225PH+p.K281KR+p.T286TA	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, K20R, V60VIM, S68SG, V90L, I135L, S162DN, K166KR, N175NH, I178L, V189I, G190GS, T200TA, R211RK, P225PH, K281KR, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.K20R+p.S68G+p.R83K+p.V118I+p.D123E+p.T165A+p.D177E+p.M184V+p.T200I+p.R211K+p.F214L+p.T215Y+p.Q278E+p.L283I+p.V292I+p.E297R	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, K20R, S68G, R83K, V118I, D123E, T165A, D177E, M184V, T200I, R211K, F214L, T215Y, Q278E, L283I, V292I, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.V35I+p.V60I+p.T69S+p.K70R+p.R83K+p.I135T+p.P170S+p.K173N+p.T200TI+p.E204EA+p.Q207QE+p.V276T	Missense mutation	2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, V35I, V60I, T69S, K70R, R83K, I135T, P170S, K173N, T200TI, E204EA, Q207QE, V276T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.E138K+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	1.5	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, E138K, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.E138K+p.M184I+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	0.6	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, E138K, M184I, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.E138K+p.M184V+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	0.9	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, E138K, M184V, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	1	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.I135T+p.G196E	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, I135T, G196E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.I135T+p.V179VD+p.I202V+p.R211EK	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, I135T, V179VD, I202V, R211EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.I135V+p.M184V+p.L210W+p.T215Y+p.T286TA	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, I135V, M184V, L210W, T215Y, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.A376T+p.A400T	Missense mutation	0.5	Susceptible	[28]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, M184V, F214L, A272P, K277R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.T139TS+p.Q197E+p.F214L	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, T139TS, Q197E, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.T200TA	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, T200TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122KE+p.K173E+p.I178IL+p.M184MV+p.G196E+p.Q207E+p.R211K	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122KE, K173E, I178IL, M184MV, G196E, Q207E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122P+p.D123E+p.I135T+p.A158S+p.S162A+p.E169D+p.D177E+p.T200A+p.F214L	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122P, D123E, I135T, A158S, S162A, E169D, D177E, T200A, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K13R+p.V35T+p.K49R+p.V60I+p.K104R+p.D121Y+p.K122E+p.E138Q+p.S162C+p.D177E+p.I180V+p.T200R+p.Q207G+p.R211K+p.V245K+p.E248D+p.D250E+p.I257IL+p.A272S+p.K281R+p.L282C+p.T286A+p.I293V	Missense mutation	0.7	Susceptible	[32]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K13R, V35T, K49R, V60I, K104R, D121Y, K122E, E138Q, S162C, D177E, I180V, T200R, Q207G, R211K, V245K, E248D, D250E, I257IL, A272S, K281R, L282C, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.A33AG+p.V35M+p.M41ML+p.D67N+p.T69D+p.K70R+p.V118I+p.K122E+p.I135T+p.I159IL+p.S162AD+p.V179I+p.M184V+p.I202V+p.F214L+p.T215F+p.K219Q+p.K223KQ+p.L228H+p.E248EK+p.A272P+p.K277R+p.A288AT	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, A33AG, V35M, M41ML, D67N, T69D, K70R, V118I, K122E, I135T, I159IL, S162AD, V179I, M184V, I202V, F214L, T215F, K219Q, K223KQ, L228H, E248EK, A272P, K277R, A288AT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.D121DY+p.K122KE+p.I135IT+p.D177DG+p.I178IM+p.V179VI+p.T200TA+p.I202IV+p.Q207QH+p.R211K	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, D121DY, K122KE, I135IT, D177DG, I178IM, V179VI, T200TA, I202IV, Q207QH, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.D121H+p.R172RK+p.D177E+p.Y188X+p.R211RK	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, D121H, R172RK, D177E, Y188X, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.I31IL+p.M41L+p.E44D+p.V60VI+p.D67N+p.L74LV+p.I94IL+p.K102KR+p.K103KN+p.V108VI+p.V118I+p.K122P+p.D123DE+p.T139X+p.E169EK+p.K173KE+p.I178L+p.M184V+p.V189VI+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219X+p.H221HY+p.L228H	Missense mutation	3.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, I31IL, M41L, E44D, V60VI, D67N, L74LV, I94IL, K102KR, K103KN, V108VI, V118I, K122P, D123DE, T139X, E169EK, K173KE, I178L, M184V, V189VI, H208HY, L210W, R211K, T215Y, K219X, H221HY, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K103N+p.V106VIM+p.K122P+p.K166R+p.E169ED+p.D177E+p.T200A+p.Q207G+p.R211K	Missense mutation	2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K103N, V106VIM, K122P, K166R, E169ED, D177E, T200A, Q207G, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K64KR+p.T69TN+p.K70KR+p.K122KE+p.D123X+p.I142IV+p.S162SN+p.M184V+p.Y188L+p.I195IT+p.G196DE+p.T200TA+p.F214FL+p.H221HY+p.K238KR	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K64KR, T69TN, K70KR, K122KE, D123X, I142IV, S162SN, M184V, Y188L, I195IT, G196DE, T200TA, F214FL, H221HY, K238KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.M41L+p.K49KR+p.V60I+p.I142V+p.M184V+p.T200TI+p.Q207E+p.R211K+p.T215F+p.V245E+p.A272P+p.L283I+p.V292I+p.E297A	Missense mutation	3.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, M41L, K49KR, V60I, I142V, M184V, T200TI, Q207E, R211K, T215F, V245E, A272P, L283I, V292I, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.M41ML+p.V60I+p.A62AV+p.K64KR+p.S68SR+p.F116FY+p.V118VI+p.I142V+p.Q151M+p.M184V+p.F214FL+p.E248X+p.D250DN+p.T286A+p.I293V	Missense mutation	2.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, M41ML, V60I, A62AV, K64KR, S68SR, F116FY, V118VI, I142V, Q151M, M184V, F214FL, E248X, D250DN, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.R83RK+p.K122E+p.I178IM+p.T200A+p.R211RK+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, R83RK, K122E, I178IM, T200A, R211RK, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.T165TI+p.T200AV+p.E204EG+p.F214FL+p.K277R+p.T286A+p.A288S	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, T165TI, T200AV, E204EG, F214FL, K277R, T286A, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35I+p.K101H+p.I135V+p.S162C+p.D177E+p.I178IV+p.G190A+p.T200E+p.E203ED+p.R211K+p.I244IV+p.V245M+p.A272P+p.T286A+p.I293V+p.E297A	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35I, K101H, I135V, S162C, D177E, I178IV, G190A, T200E, E203ED, R211K, I244IV, V245M, A272P, T286A, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35L+p.M41L+p.V60VI+p.D67N+p.T69N+p.K70R+p.A98G+p.K101Q+p.K103R+p.V106VI+p.V108VI+p.K122E+p.D123N+p.I135IM+p.E138EK+p.Q174A+p.V179D+p.Y181C+p.M184MV+p.G190A+p.L210W+p.T215F+p.K219E+p.L228HN	Missense mutation	1.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35L, M41L, V60VI, D67N, T69N, K70R, A98G, K101Q, K103R, V106VI, V108VI, K122E, D123N, I135IM, E138EK, Q174A, V179D, Y181C, M184MV, G190A, L210W, T215F, K219E, L228HN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35M+p.E53D+p.K64KR+p.V90VI+p.K103N+p.K122P+p.I135V+p.I142IV+p.R172RK+p.D177E+p.G196E+p.L228LR	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35M, E53D, K64KR, V90VI, K103N, K122P, I135V, I142IV, R172RK, D177E, G196E, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V60I+p.K64R+p.I178IM+p.T200TA	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V60I, K64R, I178IM, T200TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.A98G+p.K103N+p.I135M+p.E138A+p.S162C+p.T200A+p.R211K+p.D237N+p.K238T	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, A98G, K103N, I135M, E138A, S162C, T200A, R211K, D237N, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E28EA+p.K122E+p.D123E+p.I135T+p.S162SN+p.Q174N+p.D177E+p.M184V+p.E194T+p.G196E+p.I202IV+p.R211RK+p.R284RK+p.P294PQ	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E28EA, K122E, D123E, I135T, S162SN, Q174N, D177E, M184V, E194T, G196E, I202IV, R211RK, R284RK, P294PQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E44D+p.R83K+p.K103R+p.K166R	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E44D, R83K, K103R, K166R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E53D+p.D67N+p.T69N+p.K103N+p.F116FY+p.Q151M+p.S162SN+p.Y181C+p.M184V+p.T200TA+p.R211K+p.F214L+p.D218E+p.K219Q	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E53D, D67N, T69N, K103N, F116FY, Q151M, S162SN, Y181C, M184V, T200TA, R211K, F214L, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.I50IV+p.D67N+p.K70R+p.K122E+p.D123N+p.I135V+p.M184V+p.G196E+p.T200I+p.T215Y+p.K219E	Missense mutation	3.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, I50IV, D67N, K70R, K122E, D123N, I135V, M184V, G196E, T200I, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K103N+p.I135T+p.K173R+p.M184V+p.R211S+p.T215Y	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K103N, I135T, K173R, M184V, R211S, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K122E+p.I135V+p.E169D+p.T200E+p.R211K+p.G285GE	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K122E, I135V, E169D, T200E, R211K, G285GE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K122KE+p.I135T+p.E169ED+p.K173KT+p.Q174QK+p.N175NY+p.G196E+p.T200TA+p.F214FL+p.K277KR+p.T286TA	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K122KE, I135T, E169ED, K173KT, Q174QK, N175NY, G196E, T200TA, F214FL, K277KR, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K49R+p.L74I+p.L100I+p.K103N+p.I135IT+p.E138EG+p.S162C+p.E169D+p.D177E+p.M184V+p.T200M+p.E203Q+p.Q207E+p.R211K+p.T215Y+p.K219X+p.H221Y+p.L228LR+p.I293V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K49R, L74I, L100I, K103N, I135IT, E138EG, S162C, E169D, D177E, M184V, T200M, E203Q, Q207E, R211K, T215Y, K219X, H221Y, L228LR, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K65KR+p.D67DN+p.T69X+p.K70KR+p.K103N+p.D123E+p.I178M+p.Y181C+p.M184V+p.K219KE+p.H221Y+p.K275KR+p.R284RK	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K65KR, D67DN, T69X, K70KR, K103N, D123E, I178M, Y181C, M184V, K219KE, H221Y, K275KR, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K65R+p.S68SN+p.A98S+p.K103N+p.K122E+p.I135M+p.K166R+p.M184V+p.T200V+p.R211K	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K65R, S68SN, A98S, K103N, K122E, I135M, K166R, M184V, T200V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.A62V+p.V75T+p.K122E+p.I135IV+p.M184V+p.T200TI+p.R211RK+p.K238KR	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, A62V, V75T, K122E, I135IV, M184V, T200TI, R211RK, K238KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K103N+p.K122E+p.D177N+p.G196E+p.R211K+p.T215E	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K103N, K122E, D177N, G196E, R211K, T215E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41ML+p.V60I+p.D67DG+p.S68G+p.L74LV+p.K101KN+p.K103KN+p.V118I+p.K122E+p.D123E+p.I135T+p.K166R+p.D177E+p.Y181YC+p.G190GA+p.T200TA+p.L210LW+p.R211RK+p.T215X+p.K219KN	Missense mutation	1.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41ML, V60I, D67DG, S68G, L74LV, K101KN, K103KN, V118I, K122E, D123E, I135T, K166R, D177E, Y181YC, G190GA, T200TA, L210LW, R211RK, T215X, K219KN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.P25PS+p.V60I+p.D67N+p.T69N+p.L74V+p.V90I+p.K101P+p.K102KQ+p.K103S+p.V106I+p.K122E+p.D123X+p.I142V+p.S162Y+p.D177N+p.I178M+p.H208Y+p.T215F+p.K219Q+p.K223EQ+p.E224EK+p.A272P+p.E297D	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, P25PS, V60I, D67N, T69N, L74V, V90I, K101P, K102KQ, K103S, V106I, K122E, D123X, I142V, S162Y, D177N, I178M, H208Y, T215F, K219Q, K223EQ, E224EK, A272P, E297D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T165I+p.Q174QR+p.M184V+p.G196E+p.F214FL+p.L228LH+p.T286A+p.E297K	Missense mutation	0.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T165I, Q174QR, M184V, G196E, F214FL, L228LH, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39AE+p.M41ML+p.V60VI+p.D67N+p.K101KR+p.K103N+p.V108VI+p.I135IT+p.E169D+p.V179I+p.Y181C+p.M184MIV+p.G196E+p.Q207E+p.H208Y+p.R211K+p.T215F+p.K219Q+p.H221Y+p.L228LR	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39AE, M41ML, V60VI, D67N, K101KR, K103N, V108VI, I135IT, E169D, V179I, Y181C, M184MIV, G196E, Q207E, H208Y, R211K, T215F, K219Q, H221Y, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39N+p.V60I+p.D67N+p.T69N+p.K70R+p.K122E+p.I135T+p.Q174H+p.D177E+p.V179I+p.M184V+p.I195T+p.T200A+p.R211T+p.F214L+p.K219Q+p.A272P+p.L282F+p.A288T+p.E297K	Missense mutation	1.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39N, V60I, D67N, T69N, K70R, K122E, I135T, Q174H, D177E, V179I, M184V, I195T, T200A, R211T, F214L, K219Q, A272P, L282F, A288T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39X+p.E40EK+p.M41ML+p.K43KN+p.V60I+p.D67N+p.K103N+p.V118I+p.I142IV+p.Q174N+p.D177DN+p.V179VI+p.M184V+p.D192DN+p.G196E+p.E203A+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219KE+p.E224D+p.L228H+p.A272P+p.K277R+p.I293IV+p.P294T	Missense mutation	3.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39X, E40EK, M41ML, K43KN, V60I, D67N, K103N, V118I, I142IV, Q174N, D177DN, V179VI, M184V, D192DN, G196E, E203A, Q207E, L210W, R211K, T215Y, K219KE, E224D, L228H, A272P, K277R, I293IV, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T69TN+p.K70KR+p.V118VI+p.K122E+p.I135IL+p.T139TA+p.I178M+p.Y181X+p.R211RK+p.F214FL+p.K219KQ+p.L228LH	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T69TN, K70KR, V118VI, K122E, I135IL, T139TA, I178M, Y181X, R211RK, F214FL, K219KQ, L228LH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V179VA+p.R211K+p.P243PT+p.E248ED	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V179VA, R211K, P243PT, E248ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.D123E+p.R211RK+p.F214L+p.I293IV	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, D123E, R211RK, F214L, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.S68G+p.K122E+p.I135T+p.G190S+p.T200A+p.P225H	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, S68G, K122E, I135T, G190S, T200A, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.T69TN+p.R83K+p.K122E+p.I135V+p.N136EA+p.K166R+p.Q197L+p.I202V+p.R211RK+p.V245E+p.E248D+p.D250DN+p.A272P+p.V276I+p.T286TA+p.A288S	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, T69TN, R83K, K122E, I135V, N136EA, K166R, Q197L, I202V, R211RK, V245E, E248D, D250DN, A272P, V276I, T286TA, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.V60I+p.S68G+p.R83K+p.D123E+p.E138A+p.V179D+p.M184V+p.Y188L+p.R211K+p.T215F	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, V60I, S68G, R83K, D123E, E138A, V179D, M184V, Y188L, R211K, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35IM+p.V60I+p.R83K+p.K103N+p.I178L+p.V179I+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35IM, V60I, R83K, K103N, I178L, V179I, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35L+p.T39A+p.K101P+p.K103KR+p.K122E+p.I135R+p.D177E+p.I178M+p.T200TA+p.R211K+p.A272P+p.I293V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35L, T39A, K101P, K103KR, K122E, I135R, D177E, I178M, T200TA, R211K, A272P, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35L+p.T39A+p.M41L+p.K43E+p.E44D+p.S48T+p.D67N+p.L74V+p.K101H+p.V108I+p.V118I+p.S162Y+p.V179I+p.Y181C+p.M184V+p.G190A+p.I195K+p.G196E+p.E203K+p.H208Y+p.L210W+p.R211K+p.W212M+p.T215Y+p.K223E+p.F227Y+p.A272P+p.V276T+p.L283I+p.V292I	Missense mutation	3.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35L, T39A, M41L, K43E, E44D, S48T, D67N, L74V, K101H, V108I, V118I, S162Y, V179I, Y181C, M184V, G190A, I195K, G196E, E203K, H208Y, L210W, R211K, W212M, T215Y, K223E, F227Y, A272P, V276T, L283I, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.D86E+p.D177E+p.G196E+p.T200V+p.R211S+p.F214L	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, D86E, D177E, G196E, T200V, R211S, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.E40D+p.M41L+p.V60I+p.D67N+p.T69D+p.K70R+p.A98S+p.K104N+p.I135T+p.E138K+p.S162Y+p.R172RK+p.M184V+p.R211Q+p.T215CF+p.K219Q+p.L228H+p.V292VI	Missense mutation	2.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35T+p.T39K+p.E40D+p.V60I+p.D123DE+p.K173T+p.Q174KR+p.D177E+p.T200A+p.Q207E+p.R211K+p.F214L+p.V245Q+p.D250E+p.S251Q+p.A272P+p.V276I+p.Q278H+p.T286A+p.E291D+p.V292I+p.I293V+p.E297AV+p.L301M	Missense mutation	0.7	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35T, T39K, E40D, V60I, D123DE, K173T, Q174KR, D177E, T200A, Q207E, R211K, F214L, V245Q, D250E, S251Q, A272P, V276I, Q278H, T286A, E291D, V292I, I293V, E297AV, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35T+p.T39K+p.E40D+p.V60I+p.K102KQ+p.D121H+p.I135V+p.S162A+p.E169EA+p.K173E+p.Q174R+p.D177E+p.T200A+p.Q207E+p.R211K+p.F214L+p.V245Q+p.E248ED+p.D250V+p.S251D+p.A272P+p.Q278H+p.K281R+p.T286A+p.E291D+p.V292I+p.I293V+p.L301M	Missense mutation	1	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35T, T39K, E40D, V60I, K102KQ, D121H, I135V, S162A, E169EA, K173E, Q174R, D177E, T200A, Q207E, R211K, F214L, V245Q, E248ED, D250V, S251D, A272P, Q278H, K281R, T286A, E291D, V292I, I293V, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35T+p.T39KR+p.E40ED+p.V60I+p.K104KR+p.K122E+p.D123DE+p.I135V+p.F171Y+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207E+p.R211RK+p.F214L+p.V245HQ+p.E248ED+p.D250E+p.S251D+p.A272P+p.Q278H+p.K281KR+p.T286A+p.E291D+p.V292I+p.I293V+p.E297A+p.L301M	Missense mutation	0.8	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35T, T39KR, E40ED, V60I, K104KR, K122E, D123DE, I135V, F171Y, K173T, Q174K, D177E, I178M, T200A, Q207E, R211RK, F214L, V245HQ, E248ED, D250E, S251D, A272P, Q278H, K281KR, T286A, E291D, V292I, I293V, E297A, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VI+p.A98AEG+p.K103N+p.S134SG+p.I135M+p.E138A+p.S162SC+p.E194EK+p.T200TA+p.R211K+p.D237DN+p.K238T+p.A272PT+p.A288AS+p.I293IV+p.E297EG	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VI, A98AEG, K103N, S134SG, I135M, E138A, S162SC, E194EK, T200TA, R211K, D237DN, K238T, A272PT, A288AS, I293IV, E297EG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VI+p.T39A+p.M41ML+p.L74LI+p.V118VI+p.K122KE+p.D123E+p.I135IT+p.I142X+p.S162AD+p.K238T	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VI, T39A, M41ML, L74LI, V118VI, K122KE, D123E, I135IT, I142X, S162AD, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.D123E+p.I178M+p.T200A+p.R211K+p.T286TA+p.V292VI	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, D123E, I178M, T200A, R211K, T286TA, V292VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.K101P+p.K103N+p.K122E+p.I142V+p.V179I+p.G196E+p.L210W+p.R211K+p.T215C+p.V245Q+p.A272P+p.I293V+p.E297K	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, K101P, K103N, K122E, I142V, V179I, G196E, L210W, R211K, T215C, V245Q, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60VI+p.K103KN+p.D123E+p.I142V+p.T165L+p.M184V+p.T200A+p.R211K+p.V245MT+p.E297EA	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60VI, K103KN, D123E, I142V, T165L, M184V, T200A, R211K, V245MT, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V90I+p.A98AS+p.K122KE+p.Y181C+p.D192DN+p.T200TI+p.R211KT+p.F214L+p.L228R+p.P236PS+p.A272AS+p.A288S+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V90I, A98AS, K122KE, Y181C, D192DN, T200TI, R211KT, F214L, L228R, P236PS, A272AS, A288S, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20T+p.K49R+p.R83K+p.K122E+p.E138A+p.T200I+p.I202V+p.V245E+p.A272P+p.T296S	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20T, K49R, R83K, K122E, E138A, T200I, I202V, V245E, A272P, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22KR+p.K102KQ+p.V106I+p.I135IT+p.R211K+p.F214L+p.V292VI+p.E297EA	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22KR, K102KQ, V106I, I135IT, R211K, F214L, V292VI, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22KR+p.V35VL+p.K122KE+p.T200TA+p.F214L+p.V245EQ+p.T286TA	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22KR, V35VL, K122KE, T200TA, F214L, V245EQ, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KE+p.V35VI+p.M41ML+p.A62AV+p.D67DG+p.S68SG+p.L74LV+p.V75VL+p.K101KE+p.S162SC+p.Y181C+p.M184V+p.G190S+p.L210W+p.T215Y+p.K219KQ+p.H221HY	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KE, V35VI, M41ML, A62AV, D67DG, S68SG, L74LV, V75VL, K101KE, S162SC, Y181C, M184V, G190S, L210W, T215Y, K219KQ, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.M41L+p.L74LV+p.K103KN+p.D123E+p.I142V+p.P150PS+p.Q174QP+p.P176PS+p.I178M+p.Y181YC+p.M184V+p.I195IT+p.T200A+p.Q207E+p.T215Y+p.V245X	Missense mutation	2.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, M41L, L74LV, K103KN, D123E, I142V, P150PS, Q174QP, P176PS, I178M, Y181YC, M184V, I195IT, T200A, Q207E, T215Y, V245X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.V35I+p.K122E+p.Q174QR+p.E204EK+p.Q207QE+p.H208HY+p.R211KT	Missense mutation	1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, V35I, K122E, Q174QR, E204EK, Q207QE, H208HY, R211KT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.V35M+p.T39TA+p.R83K+p.D123E+p.S162Y+p.R211KT+p.V245VE+p.I293IV	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, V35M, T39TA, R83K, D123E, S162Y, R211KT, V245VE, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KT+p.V35I+p.R83RK+p.K101R+p.K122KE+p.D123E+p.I135T+p.K166R+p.I178M+p.V179VA+p.T200A	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KT, V35I, R83RK, K101R, K122KE, D123E, I135T, K166R, I178M, V179VA, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32Q+p.K122E+p.I135K+p.S162A+p.T200TA+p.I202V+p.V292IM	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32Q, K122E, I135K, S162A, T200TA, I202V, V292IM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32R+p.V35E+p.M41L+p.T69A+p.R83K+p.K101H+p.D123E+p.I142V+p.M164I+p.K166R+p.E169D+p.I178M+p.Y181C+p.M184V+p.H221Y+p.L228H+p.V241I+p.A272S+p.K277R+p.E297K	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32R, V35E, M41L, T69A, R83K, K101H, D123E, I142V, M164I, K166R, E169D, I178M, Y181C, M184V, H221Y, L228H, V241I, A272S, K277R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43E+p.A98S+p.K122E+p.I142V+p.M184V+p.G190A+p.E203K+p.R211RS+p.V245T+p.E297K	Missense mutation	1.2	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43E, A98S, K122E, I142V, M184V, G190A, E203K, R211RS, V245T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KR+p.K122E+p.M184V+p.T200TA+p.R211RK+p.F214FL+p.T215Y	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KR, K122E, M184V, T200TA, R211RK, F214FL, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43Q+p.K49R+p.K70R+p.K103N+p.D123E+p.I135M+p.M184V+p.T200A+p.R211K+p.K219Q+p.K223R+p.A272P+p.K275I+p.K281R+p.T286A	Missense mutation	2.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43Q, K49R, K70R, K103N, D123E, I135M, M184V, T200A, R211K, K219Q, K223R, A272P, K275I, K281R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.I50V+p.K122E+p.V179VI+p.G190A+p.F214L+p.E248D+p.A272P+p.E297K	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, I50V, K122E, V179VI, G190A, F214L, E248D, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.V111VIM+p.D121H+p.K122E+p.D123X+p.K173KE+p.D177E+p.M184MV+p.T200A+p.K201KR+p.E203ED+p.R211K+p.V245IM+p.T286TA+p.A304AE	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, V111VIM, D121H, K122E, D123X, K173KE, D177E, M184MV, T200A, K201KR, E203ED, R211K, V245IM, T286TA, A304AE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.A98S+p.K166T+p.D177E+p.Q207K+p.R211K+p.A272S+p.K281R+p.L283I+p.I293V	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, A98S, K166T, D177E, Q207K, R211K, A272S, K281R, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64KR+p.L80LF+p.D123E+p.I135T+p.S162C+p.D177DE+p.R211S+p.A272P+p.K277R+p.Q278QH+p.T286A+p.V292VI+p.I293IV	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64KR, L80LF, D123E, I135T, S162C, D177DE, R211S, A272P, K277R, Q278QH, T286A, V292VI, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64KR+p.T69KN+p.K70KN+p.L74V+p.K101KQ+p.V108VI+p.Y115F+p.K122E+p.D123E+p.S134SC+p.E138EDV+p.T139TI+p.P140PS+p.Q151M+p.I178M+p.V179I+p.Y181I+p.M184V+p.F214L+p.K219Q+p.L228H	Missense mutation	3.4	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64KR, T69KN, K70KN, L74V, K101KQ, V108VI, Y115F, K122E, D123E, S134SC, E138EDV, T139TI, P140PS, Q151M, I178M, V179I, Y181I, M184V, F214L, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64KR+p.W88C+p.K122X+p.I135IT+p.Q174R+p.I178IL+p.M184V+p.G196E+p.R211K+p.T215FS+p.V245VM+p.D250DN+p.T286TA+p.V292VI	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64KR, W88C, K122X, I135IT, Q174R, I178IL, M184V, G196E, R211K, T215FS, V245VM, D250DN, T286TA, V292VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.K103N+p.K122P+p.I135T+p.D177E+p.P225H+p.E248D+p.T286A+p.I326V+p.Y342F	Missense mutation	2.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, K103N, K122P, I135T, D177E, P225H, E248D, T286A, I326V, Y342F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.V90L+p.K122E+p.D123DN+p.V179I+p.Q207E+p.R211K+p.V245VI+p.A272P+p.L283I+p.I293V	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, V90L, K122E, D123DN, V179I, Q207E, R211K, V245VI, A272P, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.S68G+p.R83K+p.F130FL+p.T131TP+p.I135IM+p.N136NI+p.N137NT+p.Q151M+p.S162Y+p.Y181C+p.G196E+p.T200I+p.F214L	Missense mutation	2.9	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, S68G, R83K, F130FL, T131TP, I135IM, N136NI, N137NT, Q151M, S162Y, Y181C, G196E, T200I, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70~+p.K102Q+p.Q151M+p.S162C+p.K277R+p.I293V	Missense mutation	2.3	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70~, K102Q, Q151M, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70~+p.L74V+p.K102Q+p.S162C+p.K277R+p.I293V	Missense mutation	2	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70~, L74V, K102Q, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70N+p.V90I+p.A98S+p.K101KQ+p.D121H+p.K122E+p.D177DE+p.I178L+p.Y181YC+p.M184MV+p.G190GA+p.T200TA+p.W212WS	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70N, V90I, A98S, K101KQ, D121H, K122E, D177DE, I178L, Y181YC, M184MV, G190GA, T200TA, W212WS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.V90I+p.A98S+p.D123E+p.M184V+p.R211K+p.A272P+p.K281R+p.E297K	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, V90I, A98S, D123E, M184V, R211K, A272P, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70S+p.K103N+p.K122E+p.S162C+p.E169D+p.V179I+p.G196E+p.T200A+p.E204EK+p.Q207E+p.R211K+p.T215Y+p.E248D+p.A272P+p.K281R+p.A288S+p.I293V+p.E297K	Missense mutation	1.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70S, K103N, K122E, S162C, E169D, V179I, G196E, T200A, E204EK, Q207E, R211K, T215Y, E248D, A272P, K281R, A288S, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K82KR+p.D123E+p.I202V+p.R211K+p.I257IV+p.V276VI	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K82KR, D123E, I202V, R211K, I257IV, V276VI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K82KR+p.D123E+p.I202V+p.R211K	Missense mutation	1.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K82KR, D123E, I202V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L34LI+p.V60VI+p.K65R+p.S68G+p.K73M+p.V75A+p.V106VI+p.K122E+p.D123DN+p.I135T+p.S162D+p.T165TI+p.Q174H+p.Y181C+p.G190GA+p.T200A+p.Q207E+p.R211K+p.H221HY+p.V245VE+p.A272AT+p.L283I+p.T296S	Missense mutation	0.8	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L34LI, V60VI, K65R, S68G, K73M, V75A, V106VI, K122E, D123DN, I135T, S162D, T165TI, Q174H, Y181C, G190GA, T200A, Q207E, R211K, H221HY, V245VE, A272AT, L283I, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74LI+p.I132IV+p.E169D+p.Y181C+p.Q207E+p.R211K+p.T215Y+p.L228LR	Missense mutation	1.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74LI, I132IV, E169D, Y181C, Q207E, R211K, T215Y, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74V+p.K102Q+p.S162C+p.K277R+p.I293V	Missense mutation	0.5	Susceptible	[30]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74V, K102Q, S162C, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16MN+p.K20R+p.M41L+p.K43E+p.K66R+p.D67N+p.L74V+p.W88S+p.V90I+p.L100I+p.K102KR+p.K103N+p.V118VI+p.K122E+p.D123GS+p.I135L+p.S162Y+p.T200KM+p.L210W+p.T215Y+p.L228LHQ+p.V245VE+p.K277R+p.T286A	Missense mutation	2.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16MN, K20R, M41L, K43E, K66R, D67N, L74V, W88S, V90I, L100I, K102KR, K103N, V118VI, K122E, D123GS, I135L, S162Y, T200KM, L210W, T215Y, L228LHQ, V245VE, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16V+p.V21I+p.V35I+p.M41ML+p.K65R+p.K70T+p.R83K+p.Y115F+p.I142IT+p.I178MV+p.V179VI+p.Y181C+p.G190A+p.G196E+p.Q207E+p.F214FL+p.H221Y	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16V, V21I, V35I, M41ML, K65R, K70T, R83K, Y115F, I142IT, I178MV, V179VI, Y181C, G190A, G196E, Q207E, F214FL, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16V+p.V35I+p.T39A+p.D67N+p.K70R+p.R83K+p.K101A+p.V106I+p.K122P+p.I178M+p.V179I+p.M184V+p.G190A+p.R211K+p.K219Q+p.I244V+p.A272P	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16V, V35I, T39A, D67N, K70R, R83K, K101A, V106I, K122P, I178M, V179I, M184V, G190A, R211K, K219Q, I244V, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16V+p.V35T+p.I135T+p.Y181C+p.Q207E+p.F214L+p.K219H+p.V245K+p.A272P+p.K275R+p.K277R+p.Q278H+p.I293V	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16V, V35T, I135T, Y181C, Q207E, F214L, K219H, V245K, A272P, K275R, K277R, Q278H, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.K103N+p.V118I+p.K122E+p.I135T+p.M184V+p.G196E+p.I202IV+p.T215Y+p.T216L+p.P225H+p.L228R	Missense mutation	3.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, K103N, V118I, K122E, I135T, M184V, G196E, I202IV, T215Y, T216L, P225H, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A98G+p.K101E+p.K122E+p.I135T+p.E138K+p.S162Y+p.Y181C+p.T200A+p.L210F+p.T215D+p.P243T+p.V245E+p.D250E+p.A272P+p.I274V+p.Q278H+p.K281R+p.T286A+p.A288S+p.K311R	Missense mutation	0.7	Susceptible	[6]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A98G, K101E, K122E, I135T, E138K, S162Y, Y181C, T200A, L210F, T215D, P243T, V245E, D250E, A272P, I274V, Q278H, K281R, T286A, A288S, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A98S+p.K101P+p.K102Q+p.K103N+p.D123E+p.K166R+p.D177E+p.D192N+p.R211K+p.T215Y+p.V245K+p.K277R+p.R284K+p.T286A+p.E297K	Missense mutation	1	Susceptible	[6]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A98S, K101P, K102Q, K103N, D123E, K166R, D177E, D192N, R211K, T215Y, V245K, K277R, R284K, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.A98S+p.K122E+p.M184V+p.G196E+p.L210W+p.T215Y	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, A98S, K122E, M184V, G196E, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.A62V+p.V118I+p.M184V+p.L210W+p.T215Y	Missense mutation	4.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, A62V, V118I, M184V, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.V60I+p.D67N+p.L74LV+p.W88C+p.A98S+p.K102R+p.V118I+p.T139M+p.V179I+p.M184V+p.I202IV+p.Q207QK+p.H208HY+p.L210W+p.R211RKT+p.T215Y+p.K219KR+p.H221HY	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, V60I, D67N, L74LV, W88C, A98S, K102R, V118I, T139M, V179I, M184V, I202IV, Q207QK, H208HY, L210W, R211RKT, T215Y, K219KR, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K102KQ+p.K122E+p.S134SG+p.I178L+p.M184MV+p.T200E+p.R206RI+p.R211K+p.T215Y	Missense mutation	4.2	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K102KQ, K122E, S134SG, I178L, M184MV, T200E, R206RI, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K173N+p.M184V+p.I202V+p.H208Y+p.R211K+p.T215F+p.K219Q+p.L228R+p.A272S+p.T286A	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K173N, M184V, I202V, H208Y, R211K, T215F, K219Q, L228R, A272S, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.E44A+p.E53EG+p.D67N+p.L74I+p.V75M+p.H96HN+p.L100I+p.K103N+p.V118I+p.K122E+p.A158S+p.S162D+p.M184V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219KN	Missense mutation	4	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, E44A, E53EG, D67N, L74I, V75M, H96HN, L100I, K103N, V118I, K122E, A158S, S162D, M184V, Q207E, L210W, R211K, T215Y, K219KN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.T58TI+p.D67N+p.L74I+p.K103KN+p.K122KE+p.N137NKT+p.N147NK+p.P150PR+p.F171FL+p.M184V+p.T200R+p.R211T+p.T215Y+p.D218E	Missense mutation	4.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, T58TI, D67N, L74I, K103KN, K122KE, N137NKT, N147NK, P150PR, F171FL, M184V, T200R, R211T, T215Y, D218E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.V60I+p.D67N+p.T69D+p.K70KR+p.L74V+p.L100I+p.K103N+p.I135L+p.E138G+p.I178L+p.M184V+p.G196E+p.Q207E+p.T215Y+p.V245E+p.A272P+p.K277KR+p.Q278QH+p.T286A+p.E291A+p.I293V	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, V60I, D67N, T69D, K70KR, L74V, L100I, K103N, I135L, E138G, I178L, M184V, G196E, Q207E, T215Y, V245E, A272P, K277KR, Q278QH, T286A, E291A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K49KR+p.V60I+p.V90VA+p.A98S+p.S117SP+p.K122E+p.D123E+p.S134SC+p.V179VI+p.Y181C+p.M184V+p.T200TA+p.L210W+p.T215Y+p.K219E+p.L228R+p.H235HL+p.V245E	Missense mutation	1.2	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K49KR, V60I, V90VA, A98S, S117SP, K122E, D123E, S134SC, V179VI, Y181C, M184V, T200TA, L210W, T215Y, K219E, L228R, H235HL, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K49KR+p.V60VI+p.L74V+p.V118I+p.D123E+p.I135T+p.N136NT+p.T165TI+p.D177E+p.V179I+p.M184V+p.L210W+p.T215Y+p.V245E+p.A272S+p.K277R	Missense mutation	2.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K49KR, V60VI, L74V, V118I, D123E, I135T, N136NT, T165TI, D177E, V179I, M184V, L210W, T215Y, V245E, A272S, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K70R+p.V118I+p.M184V+p.H208Y+p.T215Y+p.K219E	Missense mutation	4.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K70R, V118I, M184V, H208Y, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74V+p.K103N+p.K122E+p.I135T+p.Y181C+p.M184V+p.T200A+p.T215FY+p.K238N+p.A272P	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74V, K103N, K122E, I135T, Y181C, M184V, T200A, T215FY, K238N, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74V+p.R83K+p.D123DE+p.V179VI+p.Y181C+p.M184V+p.G196E+p.T200TA+p.R211RK+p.T215Y+p.A272P+p.E297K	Missense mutation	0.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74V, R83K, D123DE, V179VI, Y181C, M184V, G196E, T200TA, R211RK, T215Y, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.R83K+p.D123E+p.M184V+p.G196E+p.R211K+p.T215Y+p.A272P+p.E297K	Missense mutation	2.2	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, R83K, D123E, M184V, G196E, R211K, T215Y, A272P, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68R+p.L74V+p.A98S+p.K101Q+p.K103N+p.K122E+p.D123DN+p.I135T+p.Q174R+p.D177E+p.I178M+p.M184V+p.T200E+p.Q207R+p.L210W+p.R211Q+p.T215Y+p.K219KN	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68R, L74V, A98S, K101Q, K103N, K122E, D123DN, I135T, Q174R, D177E, I178M, M184V, T200E, Q207R, L210W, R211Q, T215Y, K219KN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.L74V+p.K103N+p.V118I+p.K122E+p.I135T+p.M184V+p.L210W+p.T215Y+p.D218E+p.K219Q+p.A272P+p.K277R+p.E297Q	Missense mutation	3.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, L74V, K103N, V118I, K122E, I135T, M184V, L210W, T215Y, D218E, K219Q, A272P, K277R, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.D67N+p.S68G+p.E79EK+p.T139A+p.T200I+p.I202V+p.R211RK+p.T215S+p.K219E+p.L228H+p.V245E+p.A272P+p.T286TA+p.P294T+p.E297Q	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, D67N, S68G, E79EK, T139A, T200I, I202V, R211RK, T215S, K219E, L228H, V245E, A272P, T286TA, P294T, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.Y56YC+p.K102Q+p.K103KN+p.K122E+p.I142IT+p.N175H+p.D177E+p.I178L+p.M184V+p.T200E+p.K201I+p.T215Y+p.A272G+p.A288S+p.I293V	Missense mutation	3.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, Y56YC, K102Q, K103KN, K122E, I142IT, N175H, D177E, I178L, M184V, T200E, K201I, T215Y, A272G, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.D67N+p.T69DY+p.K70R+p.D123E+p.I178M+p.M184V+p.I202V+p.Q207A+p.R211K+p.T215F+p.K219Q+p.L228H+p.V245H	Missense mutation	2.9	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, D67N, T69DY, K70R, D123E, I178M, M184V, I202V, Q207A, R211K, T215F, K219Q, L228H, V245H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.K46KQ+p.V60I+p.A62AV+p.D67DG+p.S68G+p.K70KR+p.K122E+p.I135T+p.E138A+p.T165I+p.M184MV+p.G190GA+p.I195L+p.G196E+p.Q197QR+p.Q207A+p.R211K+p.T215X+p.K219KQ+p.A288AS	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, K46KQ, V60I, A62AV, D67DG, S68G, K70KR, K122E, I135T, E138A, T165I, M184MV, G190GA, I195L, G196E, Q197QR, Q207A, R211K, T215X, K219KQ, A288AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N57ND+p.V60I+p.D67DN+p.K70GR+p.V118VI+p.K166KR+p.M184V+p.E203EK+p.R211RK+p.F214L+p.K219Q+p.I293V	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N57ND, V60I, D67DN, K70GR, V118VI, K166KR, M184V, E203EK, R211RK, F214L, K219Q, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P19PL+p.K20R+p.V60L+p.K122E+p.D123DN+p.I135T+p.F171FC+p.Q197E+p.T200X+p.F214L+p.E248EK+p.A272P+p.T286TA+p.V292I+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P19PL, K20R, V60L, K122E, D123DN, I135T, F171FC, Q197E, T200X, F214L, E248EK, A272P, T286TA, V292I, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K20R+p.D67N+p.T69D+p.K70R+p.L74V+p.L100I+p.K103N+p.K122E+p.G196E+p.T215F+p.D218E+p.K219Q+p.E297K	Missense mutation	3.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K20R, D67N, T69D, K70R, L74V, L100I, K103N, K122E, G196E, T215F, D218E, K219Q, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K64H+p.D67G+p.T69DN+p.K70R+p.L74LI+p.V118I+p.K122E+p.I135T+p.M184V+p.T200AS+p.F214L+p.T215F+p.D218E+p.K219Q	Missense mutation	3.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K64H, D67G, T69DN, K70R, L74LI, V118I, K122E, I135T, M184V, T200AS, F214L, T215F, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4ED+p.K20R+p.V35I+p.M41ML+p.V60VI+p.D67N+p.T69D+p.V108VI+p.E203D+p.Q207QE+p.H208Y+p.T215D	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4ED, K20R, V35I, M41ML, V60VI, D67N, T69D, V108VI, E203D, Q207QE, H208Y, T215D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4PHQ+p.V8VI+p.P9PS+p.V35VI+p.F87L+p.K102E+p.K103N+p.V118I+p.K122E+p.I135IT+p.S162HY+p.R172RK+p.Q174E+p.Y188L+p.D192DN+p.R211S+p.F214L+p.P225PS+p.E297EK	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4PHQ, V8VI, P9PS, V35VI, F87L, K102E, K103N, V118I, K122E, I135IT, S162HY, R172RK, Q174E, Y188L, D192DN, R211S, F214L, P225PS, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4PS+p.M41L+p.K43N+p.E44D+p.D67N+p.V75VL+p.K102KR+p.K103N+p.V118I+p.K122EG+p.Q174N+p.I178M+p.M184V+p.E203EK+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219E	Missense mutation	3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4PS, M41L, K43N, E44D, D67N, V75VL, K102KR, K103N, V118I, K122EG, Q174N, I178M, M184V, E203EK, Q207E, L210W, R211K, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4PST+p.K20KR+p.M41L+p.D67N+p.L74LV+p.K82KR+p.K122E+p.I135V+p.S163SC+p.V179E+p.G190S+p.R211S+p.T215Y+p.S251H+p.K277KR+p.T286A	Missense mutation	4	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4PST, K20KR, M41L, D67N, L74LV, K82KR, K122E, I135V, S163SC, V179E, G190S, R211S, T215Y, S251H, K277KR, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.V35T+p.E36ED+p.T39TA+p.I135V+p.S162A+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207E+p.V245EK+p.K281KR+p.T286A+p.E291D+p.V292I+p.I293V+p.P294T	Missense mutation	1.1	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, V35T, E36ED, T39TA, I135V, S162A, K173T, Q174K, D177E, I178M, T200A, Q207E, V245EK, K281KR, T286A, E291D, V292I, I293V, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4T+p.V35T+p.V60I+p.K122E+p.D123S+p.F171Y+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207E+p.V245Q+p.E248N+p.D250E+p.S251N+p.A272P+p.K277R+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	0.6	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4T, V35T, V60I, K122E, D123S, F171Y, K173T, Q174K, D177E, I178M, T200A, Q207E, V245Q, E248N, D250E, S251N, A272P, K277R, T286A, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P95PS+p.D123E+p.R211K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P95PS, D123E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9PS+p.M41L+p.L74V+p.K103N+p.V108I+p.I142V+p.M184V+p.R211T+p.T215Y+p.A272P	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9PS, M41L, L74V, K103N, V108I, I142V, M184V, R211T, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P9PS+p.V35I+p.D67N+p.K70R+p.A98S+p.K101D+p.K122E+p.D123DN+p.I135KR+p.M184V+p.G190A+p.T215V+p.K219E+p.D237E+p.V245E+p.T286TA+p.V292I+p.I293V+p.E297R	Missense mutation	2.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P9PS, V35I, D67N, K70R, A98S, K101D, K122E, D123DN, I135KR, M184V, G190A, T215V, K219E, D237E, V245E, T286TA, V292I, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q174D+p.G196E+p.T200TA+p.W212WR+p.P243S+p.T286A+p.I293V	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q174D, G196E, T200TA, W212WR, P243S, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R211K+p.F214FL	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.G196GE+p.T200R+p.K201KR+p.V245KR	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, G196GE, T200R, K201KR, V245KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.I135T+p.S162Y+p.I178L+p.D192DN+p.T200TA+p.R211RG+p.F214L+p.I244V+p.V245E+p.A272P+p.K277R+p.I293V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, I135T, S162Y, I178L, D192DN, T200TA, R211RG, F214L, I244V, V245E, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K101E+p.D123E+p.I202V+p.A272P+p.K281R+p.P294T+p.E297R	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K101E, D123E, I202V, A272P, K281R, P294T, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K102Q+p.S162A+p.T200A+p.A272P+p.K277R+p.E297A	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K102Q, S162A, T200A, A272P, K277R, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K103N+p.D123E+p.E138A+p.S162C+p.I178L+p.V179I+p.G196R+p.R211K+p.P217PS+p.A272P	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K103N, D123E, E138A, S162C, I178L, V179I, G196R, R211K, P217PS, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K104KN+p.K122E+p.D123E+p.I178IM+p.T200A	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K104KN, K122E, D123E, I178IM, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K122E+p.F130FL+p.S163T+p.T165TI+p.I178M	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K122E, F130FL, S163T, T165TI, I178M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.P170PL+p.D177E+p.I178IM+p.M184MIV+p.I195IV+p.T200A+p.Q207E+p.R211RK+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, P170PL, D177E, I178IM, M184MIV, I195IV, T200A, Q207E, R211RK, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.P176PS+p.D177DE+p.I178M+p.T200TI+p.R211RK	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, P176PS, D177DE, I178M, T200TI, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.T165I+p.R211K+p.K277R+p.E297V	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, T165I, R211K, K277R, E297V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.T200A+p.R211G+p.V245E+p.D250E+p.I293V	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, T200A, R211G, V245E, D250E, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.I178M+p.V189I+p.Q207G+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, I178M, V189I, Q207G, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.K122KE+p.M184MV+p.T200A+p.R211K+p.V245E+p.T286A+p.I293IVM+p.E297A	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, K122KE, M184MV, T200A, R211K, V245E, T286A, I293IVM, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.S134SN+p.I135V+p.S162A+p.Q197QH+p.T200TA+p.K201KR+p.A288S+p.V292I	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, S134SN, I135V, S162A, Q197QH, T200TA, K201KR, A288S, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S162C+p.E169D+p.Q182QR+p.T200A+p.I202V+p.R211K+p.K277R+p.Q278H+p.T286A+p.I293V+p.E297K+p.P313T+p.D324E+p.I329L+p.Q334E	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S162C, E169D, Q182QR, T200A, I202V, R211K, K277R, Q278H, T286A, I293V, E297K, P313T, D324E, I329L, Q334E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.K20KR+p.T39TA+p.M41ML+p.D67N+p.K70R+p.T84V+p.A98S+p.V179VI+p.G190A+p.L210F+p.R211K+p.T215Y+p.K219E+p.A272P	Missense mutation	4.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, K20KR, T39TA, M41ML, D67N, K70R, T84V, A98S, V179VI, G190A, L210F, R211K, T215Y, K219E, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3N+p.V21A+p.E28A+p.T39I+p.L74V+p.Y115F+p.K122E+p.D123E+p.I135T+p.M184V+p.G190S+p.G196E+p.F214L+p.A272P+p.L283I+p.E297V	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3N, V21A, E28A, T39I, L74V, Y115F, K122E, D123E, I135T, M184V, G190S, G196E, F214L, A272P, L283I, E297V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.T27S+p.V35I+p.M41L+p.R83RK+p.V90VI+p.K122KE+p.D123DE+p.S162HY+p.I178M+p.M184MV+p.T200A+p.Q207E+p.L210LW+p.T215SY+p.K281KR	Missense mutation	3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, T27S, V35I, M41L, R83RK, V90VI, K122KE, D123DE, S162HY, I178M, M184MV, T200A, Q207E, L210LW, T215SY, K281KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48T+p.D67N+p.T69DN+p.K70GR+p.L74V+p.K101KR+p.K104KR+p.D121Y+p.K122E+p.D123E+p.E169D+p.D177E+p.M184V+p.G190E+p.T200A+p.Q207K+p.F214L+p.K219EQ+p.K275KR+p.V276VI+p.P294T+p.E297KR	Missense mutation	1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48T, D67N, T69DN, K70GR, L74V, K101KR, K104KR, D121Y, K122E, D123E, E169D, D177E, M184V, G190E, T200A, Q207K, F214L, K219EQ, K275KR, V276VI, P294T, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48T+p.L74V+p.L100I+p.K103N+p.K122E+p.I135LV+p.T200A+p.T215C+p.L228LH+p.D250X	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48T, L74V, L100I, K103N, K122E, I135LV, T200A, T215C, L228LH, D250X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.T69A+p.K122E+p.I135T+p.E204Q+p.R211K+p.V245E+p.P294T	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, T69A, K122E, I135T, E204Q, R211K, V245E, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27P+p.V35IT+p.V60VI+p.K122E+p.I135V+p.G196GE+p.T200A+p.Q207E+p.E248DN+p.K281KR	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27P, V35IT, V60VI, K122E, I135V, G196GE, T200A, Q207E, E248DN, K281KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.V35I+p.M41L+p.E44D+p.T69D+p.V108VI+p.D121H+p.K122E+p.D177E+p.V179VI+p.Y181C+p.T200A+p.Q207E+p.H208HY+p.L210LW+p.R211RK+p.T215Y+p.L228HR	Missense mutation	3.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, V35I, M41L, E44D, T69D, V108VI, D121H, K122E, D177E, V179VI, Y181C, T200A, Q207E, H208HY, L210LW, R211RK, T215Y, L228HR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40D+p.R83K+p.K103KN+p.V179I+p.Y181C+p.T215Y+p.K223KQ+p.L228R	Missense mutation	2.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40D, R83K, K103KN, V179I, Y181C, T215Y, K223KQ, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.E40EK+p.D67N+p.K70G+p.K103N+p.V118I+p.K122E+p.S134SG+p.S162A+p.E169D+p.G196E+p.R211A+p.F214L+p.T215F+p.K219Q+p.L228H+p.V245E	Missense mutation	0.8	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, E40EK, D67N, K70G, K103N, V118I, K122E, S134SG, S162A, E169D, G196E, R211A, F214L, T215F, K219Q, L228H, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K49KR+p.K65R+p.S68G+p.K70T+p.V108I+p.D123E+p.I135IT+p.I142V+p.D177E+p.Y181V+p.M184V+p.T200A+p.H221HY+p.M230ML	Missense mutation	2.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K49KR, K65R, S68G, K70T, V108I, D123E, I135IT, I142V, D177E, Y181V, M184V, T200A, H221HY, M230ML.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K49R+p.V118I+p.K122E+p.I135T+p.G196E+p.R211K+p.F214L+p.L228H	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K49R, V118I, K122E, I135T, G196E, R211K, F214L, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.K70G+p.L74LV+p.K122P+p.D123E+p.N137S+p.I142V+p.K166R+p.D177E+p.G190S+p.E203EG+p.T215F+p.D218E+p.K219Q+p.L228LR+p.A272P+p.V292I+p.E297A	Missense mutation	2.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, K70G, L74LV, K122P, D123E, N137S, I142V, K166R, D177E, G190S, E203EG, T215F, D218E, K219Q, L228LR, A272P, V292I, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43KE+p.V60VI+p.S68SG+p.L74V+p.V118I+p.K122E+p.I142V+p.S162Y+p.I178IM+p.M184V+p.T200A+p.T215Y	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43KE, V60VI, S68SG, L74V, V118I, K122E, I142V, S162Y, I178IM, M184V, T200A, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43KQ+p.V60VI+p.L74V+p.K103N+p.V118VI+p.D121Y+p.K122E+p.I142V+p.S162Y+p.M184V+p.T200A+p.Q207N+p.L210W+p.R211K+p.T215Y	Missense mutation	3.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43KQ, V60VI, L74V, K103N, V118VI, D121Y, K122E, I142V, S162Y, M184V, T200A, Q207N, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.L74V+p.A98G+p.K103N+p.V108I+p.I142V+p.Y181C+p.R211K+p.T215Y+p.H221Y+p.F227FL	Missense mutation	3.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, L74V, A98G, K103N, V108I, I142V, Y181C, R211K, T215Y, H221Y, F227FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.L74V+p.V75S+p.R83K+p.V90I+p.K101P+p.K102H+p.K104KR+p.K122E+p.D123DN+p.I135T+p.E138ED+p.T139TS+p.S162Y+p.V179I+p.M184V+p.R206X+p.L210LF+p.T215Y+p.K223KQ+p.H235HL	Missense mutation	3.7	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, L74V, V75S, R83K, V90I, K101P, K102H, K104KR, K122E, D123DN, I135T, E138ED, T139TS, S162Y, V179I, M184V, R206X, L210LF, T215Y, K223KQ, H235HL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.V60I+p.S68SG+p.L74V+p.D121H+p.K122E+p.I142V+p.D177E+p.I178M+p.M184V+p.Y188I+p.G196E+p.T200K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H	Missense mutation	3.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, V60I, S68SG, L74V, D121H, K122E, I142V, D177E, I178M, M184V, Y188I, G196E, T200K, Q207E, L210W, R211K, T215Y, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39N+p.M41L+p.K43E+p.A98G+p.V118I+p.K122P+p.D177E+p.V179I+p.M184MV+p.T200TI+p.Q207E+p.H208HY+p.L210W+p.R211K+p.T215Y+p.L228LHQ+p.T286TA	Missense mutation	2.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39S+p.M41L+p.L74V+p.K101Q+p.Y115F+p.K122E+p.I132IL+p.E138EG+p.S163C+p.T165TI+p.Q174QK+p.Y181C+p.M184V+p.L187LS+p.V189I+p.G190S+p.D192DH+p.T200A+p.E203V+p.Q207E+p.T215Y+p.K219E	Missense mutation	0.8	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39S, M41L, L74V, K101Q, Y115F, K122E, I132IL, E138EG, S163C, T165TI, Q174QK, Y181C, M184V, L187LS, V189I, G190S, D192DH, T200A, E203V, Q207E, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.M41L+p.K64H+p.L74V+p.V90I+p.K103N+p.V108I+p.V118I+p.I135V+p.I142V+p.D177E+p.I178M+p.Q207H+p.L210LW+p.R211K+p.T215Y+p.L228LF	Missense mutation	2.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, M41L, K64H, L74V, V90I, K103N, V108I, V118I, I135V, I142V, D177E, I178M, Q207H, L210LW, R211K, T215Y, L228LF.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TI+p.A98S+p.D123E+p.R211K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TI, A98S, D123E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TK+p.M41L+p.E44ED+p.L74V+p.V118VI+p.P140PT+p.V179I+p.Y181V+p.M184V+p.L210LW+p.R211RK+p.T215Y+p.K219K*+p.V245K	Missense mutation	1.6	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TK, M41L, E44ED, L74V, V118VI, P140PT, V179I, Y181V, M184V, L210LW, R211RK, T215Y, K219K*, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39X+p.M41L+p.K43KQ+p.E44D+p.D67G+p.T69D+p.V75M+p.F77L+p.A98S+p.K101Q+p.V111I+p.V118I+p.K122E+p.A158S+p.S162X+p.M184V+p.I202IV+p.L210W+p.T215H+p.T286A+p.I293V+p.E297K	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39X, M41L, K43KQ, E44D, D67G, T69D, V75M, F77L, A98S, K101Q, V111I, V118I, K122E, A158S, S162X, M184V, I202IV, L210W, T215H, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69A+p.Y115F+p.V118I+p.K122KE+p.I135IV+p.Q174H+p.M184V+p.G196E+p.R211G+p.F214L+p.L228H+p.V245T+p.A272P+p.K277R+p.D324E+p.A360T+p.A371V+p.I375V+p.A376S+p.A400T+p.V435E+p.R448G+p.L452V+p.D460N+p.T470A+p.H483Y+p.Q547R+p.A554S	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69A, Y115F, V118I, K122KE, I135IV, Q174H, M184V, G196E, R211G, F214L, L228H, V245T, A272P, K277R, D324E, A360T, A371V, I375V, A376S, A400T, V435E, R448G, L452V, D460N, T470A, H483Y, Q547R, A554S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69N+p.K70R+p.D121H+p.K122E+p.I135V+p.I142V+p.M184V+p.K219Q+p.E233EK	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69N, K70R, D121H, K122E, I135V, I142V, M184V, K219Q, E233EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69N+p.K70R+p.K103N+p.M184V+p.R211K	Missense mutation	1.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69N, K70R, K103N, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69TN+p.P95PS+p.K122E+p.G196E+p.T200A+p.Q207E	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69TN, P95PS, K122E, G196E, T200A, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T7TA+p.K20KR+p.V60I+p.R83K+p.V90I+p.K122P+p.S162Y+p.D177DE+p.I180IV+p.T200AE+p.R211RK+p.V245VE+p.E297EA	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T7TA, K20KR, V60I, R83K, V90I, K122P, S162Y, D177DE, I180IV, T200AE, R211RK, V245VE, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T7TA+p.V35VIM+p.M41MIL+p.K102KR+p.K104N+p.D121H+p.K122E+p.D177E+p.T200A+p.R211K+p.P243PT+p.V245KM	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T7TA, V35VIM, M41MIL, K102KR, K104N, D121H, K122E, D177E, T200A, R211K, P243PT, V245KM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T7TIM+p.V35I+p.T39TA+p.M41L+p.K43N+p.S68G+p.L74V+p.V90VI+p.K103N+p.V118I+p.K122E+p.I142T+p.V179VI+p.Y181YC+p.M184V+p.T200TI+p.E203ED+p.L210W+p.R211K+p.T215Y+p.K219KN	Missense mutation	1.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T7TIM, V35I, T39TA, M41L, K43N, S68G, L74V, V90VI, K103N, V118I, K122E, I142T, V179VI, Y181YC, M184V, T200TI, E203ED, L210W, R211K, T215Y, K219KN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V106I+p.S162HY+p.R211G+p.V245MR+p.E248X	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V106I, S162HY, R211G, V245MR, E248X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V111VL+p.K122KE+p.D123E+p.T131TP+p.S134SG+p.E138ED+p.T139TI+p.I142V+p.I178IM+p.G196E+p.T200A+p.Q207EG+p.V245M	Missense mutation	0.8	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V111VL, K122KE, D123E, T131TP, S134SG, E138ED, T139TI, I142V, I178IM, G196E, T200A, Q207EG, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V118I+p.K122R+p.D123E+p.G196E+p.Q207E+p.R211K+p.F214L	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V118I, K122R, D123E, G196E, Q207E, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.D67DN+p.D123E+p.E169D+p.Q174K+p.D177E+p.M184V+p.Q207E+p.R211K+p.D218E+p.A272P+p.E297A	Missense mutation	1.1	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, D67DN, D123E, E169D, Q174K, D177E, M184V, Q207E, R211K, D218E, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.D67N+p.K70KR+p.K103KN+p.D123E+p.E169D+p.Q174K+p.D177E+p.Y181YC+p.M184MV+p.Q207E+p.R211K+p.D218E+p.A272P+p.E297A	Missense mutation	3.3	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, D67N, K70KR, K103KN, D123E, E169D, Q174K, D177E, Y181YC, M184MV, Q207E, R211K, D218E, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.I31L+p.V75I+p.D123E+p.T139A+p.I178IM+p.V179VI+p.Y181C+p.G196E+p.R211K+p.F214FL+p.V245E+p.E248D+p.I293V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, I31L, V75I, D123E, T139A, I178IM, V179VI, Y181C, G196E, R211K, F214FL, V245E, E248D, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.V35L+p.M41ML+p.D123E+p.V179VI+p.M184V+p.L210W+p.R211K+p.T215Y+p.D218R	Missense mutation	1.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, V35L, M41ML, D123E, V179VI, M184V, L210W, R211K, T215Y, D218R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.V35T+p.T39M+p.M41L+p.V60I+p.V75M+p.K103N+p.K122E+p.D123S+p.I135IT+p.Q174KR+p.D177E+p.I178M+p.M184V+p.T200A+p.Q207E+p.R211K+p.T215Y	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, V35T, T39M, M41L, V60I, V75M, K103N, K122E, D123S, I135IT, Q174KR, D177E, I178M, M184V, T200A, Q207E, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.R83RK+p.V90I+p.K103KN+p.K122KE+p.D123DE+p.S162A+p.Q174H+p.G196E+p.T200TA+p.I202V	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, R83RK, V90I, K103KN, K122KE, D123DE, S162A, Q174H, G196E, T200TA, I202V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V35I+p.E36K+p.V60I+p.D67N+p.K70R+p.L74I+p.K102K.+p.Y115F+p.D121H+p.K122E+p.I135T+p.D177E+p.I180V+p.M184V+p.T200A+p.D218E+p.K219Q+p.L228H	Missense mutation	2.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V35I, E36K, V60I, D67N, K70R, L74I, K102K., Y115F, D121H, K122E, I135T, D177E, I180V, M184V, T200A, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V35T+p.E40ED+p.V60I+p.K122E+p.D123S+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207A+p.R211X+p.V245Q+p.E248D+p.D250E+p.A272P+p.T286A+p.E291D+p.V292I+p.I293IV	Missense mutation	0.8	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V35T, E40ED, V60I, K122E, D123S, K173T, Q174K, D177E, I178M, T200A, Q207A, R211X, V245Q, E248D, D250E, A272P, T286A, E291D, V292I, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V60I+p.D121H+p.K122E+p.I135T+p.I142V+p.M184MV+p.G196E+p.V245K	Missense mutation	0.7	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V60I, D121H, K122E, I135T, I142V, M184MV, G196E, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.A62V+p.V75I+p.K101S+p.K122KE+p.I135T+p.I142IL+p.K166R+p.G190S+p.R211K	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, A62V, V75I, K101S, K122KE, I135T, I142IL, K166R, G190S, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D123E+p.I135T+p.Q174N+p.D177E+p.I202IV+p.F214FL	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D123E, I135T, Q174N, D177E, I202IV, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.G196GE+p.Q197QE+p.R211RK+p.A272P+p.V292I+p.E297K	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, G196GE, Q197QE, R211RK, A272P, V292I, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.I135L+p.I142V+p.Q145E+p.M184V+p.T200A+p.R211K+p.D250E+p.T286A+p.E297R+p.L301I	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, I135L, I142V, Q145E, M184V, T200A, R211K, D250E, T286A, E297R, L301I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K101Q+p.K103N+p.K122E+p.I135K+p.G196E+p.V245E+p.E248D+p.A272P+p.E297R	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K101Q, K103N, K122E, I135K, G196E, V245E, E248D, A272P, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K102Q+p.K104N+p.D123E+p.I142V+p.K166R+p.G196E+p.T200A+p.R211K	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K102Q, K104N, D123E, I142V, K166R, G196E, T200A, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K122KE+p.S162X+p.I202V+p.R211K+p.F214L+p.V245T+p.A272P	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K122KE, S162X, I202V, R211K, F214L, V245T, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K43Q+p.A62V+p.W88S+p.S105SL+p.D123DE+p.I135T+p.S162Y+p.I178L+p.M184V+p.E203D+p.Q207E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K223T+p.L228R	Missense mutation	2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K43Q, A62V, W88S, S105SL, D123DE, I135T, S162Y, I178L, M184V, E203D, Q207E, H208Y, L210W, R211K, T215Y, K223T, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K49KR+p.R83K+p.W88WV+p.I135V+p.T215TA+p.H221HY	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K49KR, R83K, W88WV, I135V, T215TA, H221HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K49KR+p.V75M+p.K101KR+p.K103N+p.K104KR+p.K122E+p.I135T+p.T200I	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K49KR, V75M, K101KR, K103N, K104KR, K122E, I135T, T200I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K64KR+p.R83K+p.K103N+p.D123E+p.I135T+p.I178M+p.K277R+p.I293V+p.P321S+p.I329V+p.Q334E	Missense mutation	2.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K64KR, R83K, K103N, D123E, I135T, I178M, K277R, I293V, P321S, I329V, Q334E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K70KT+p.K103N+p.K122P+p.D177N+p.V179E+p.I180V+p.M184MV+p.G196GR+p.Q207E+p.R211K+p.T215Y+p.M230L+p.K238I	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K70KT, K103N, K122P, D177N, V179E, I180V, M184MV, G196GR, Q207E, R211K, T215Y, M230L, K238I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K70R+p.R83K+p.I135T+p.S162C+p.L210LW+p.T240TK+p.K277R+p.T286A	Missense mutation	4.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K70R, R83K, I135T, S162C, L210LW, T240TK, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.K103KN+p.K122E+p.I142V+p.Q197L+p.T200A+p.Q207E+p.L228R+p.I244IV+p.P294TS	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, K103KN, K122E, I142V, Q197L, T200A, Q207E, L228R, I244IV, P294TS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.L74V+p.L100I+p.K103NS+p.V111VI+p.V118VI+p.K122E+p.K173KR+p.D177DG+p.M184V+p.T200TA+p.E203EK+p.Q207X+p.L210W+p.R211RK+p.T215Y+p.K219N	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, L74V, L100I, K103NS, V111VI, V118VI, K122E, K173KR, D177DG, M184V, T200TA, E203EK, Q207X, L210W, R211RK, T215Y, K219N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41ML+p.D67G+p.S68G+p.K70R+p.K122E+p.I135T+p.M184V+p.T215F+p.K219E+p.P225H	Missense mutation	3.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41ML, D67G, S68G, K70R, K122E, I135T, M184V, T215F, K219E, P225H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.D123E+p.I135T+p.D177E+p.V245K+p.A272P+p.Q278Y	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, D123E, I135T, D177E, V245K, A272P, Q278Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.D123E+p.I135T+p.R211K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, D123E, I135T, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S68G+p.V90I+p.K103N+p.F116Y+p.D121H+p.T139TA+p.Q151M+p.K173Q+p.Q174R+p.D177E+p.I178L+p.Y181C+p.G196E+p.T200A+p.R211RT+p.F214L	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S68G, V90I, K103N, F116Y, D121H, T139TA, Q151M, K173Q, Q174R, D177E, I178L, Y181C, G196E, T200A, R211RT, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39L+p.K103KN+p.D123E+p.G196E+p.Q207K+p.L210M+p.R211A	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39L, K103KN, D123E, G196E, Q207K, L210M, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39S+p.M41L+p.K43Q+p.V60I+p.D67N+p.T69D+p.A98S+p.V118I+p.M184V+p.T200TA+p.E203D+p.L210W+p.R211K+p.T215Y+p.K219KR+p.V245Q+p.A272P+p.T286TA+p.A288G+p.I293V+p.T296S	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39S, M41L, K43Q, V60I, D67N, T69D, A98S, V118I, M184V, T200TA, E203D, L210W, R211K, T215Y, K219KR, V245Q, A272P, T286TA, A288G, I293V, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.V108I+p.I135T+p.S162A+p.K173T+p.Q174K+p.D177E+p.I178M+p.V189I+p.T200A+p.Q207E+p.V245Q+p.E248D+p.K281R+p.T286A+p.E291D+p.V292I+p.I293V+p.P294T	Missense mutation	1	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, V108I, I135T, S162A, K173T, Q174K, D177E, I178M, V189I, T200A, Q207E, V245Q, E248D, K281R, T286A, E291D, V292I, I293V, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.V118I+p.D123E+p.D177E+p.R211K+p.F214L+p.V245M+p.E248D+p.D250E+p.A288S	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, V118I, D123E, D177E, R211K, F214L, V245M, E248D, D250E, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.W88C+p.K122E+p.I178M+p.S251T+p.T286A+p.T296S	Missense mutation	0.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, W88C, K122E, I178M, S251T, T286A, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.D123E+p.I135T+p.K166KR+p.D177DE+p.R211RK+p.V245M+p.I293V	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, D123E, I135T, K166KR, D177DE, R211RK, V245M, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.E53ED+p.K103N+p.D123E+p.M184V+p.Q207E+p.R211K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, E53ED, K103N, D123E, M184V, Q207E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.T39TA+p.K49R+p.R83K+p.V90VI+p.I135T+p.Y144F+p.I178IV+p.G196GE+p.T200TA+p.R211K+p.Q278QH+p.E297EK	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, T39TA, K49R, R83K, V90VI, I135T, Y144F, I178IV, G196GE, T200TA, R211K, Q278QH, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.T39TA+p.R83K+p.K102KQ+p.K103N+p.D123E+p.S162C+p.D177E+p.I178R+p.V179F+p.R211RK+p.V245EQ+p.D250E+p.K277R+p.T286A+p.I293V+p.M357AS+p.E370ED+p.T377TMI	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, T39TA, R83K, K102KQ, K103N, D123E, S162C, D177E, I178R, V179F, R211RK, V245EQ, D250E, K277R, T286A, I293V, M357AS, E370ED, T377TMI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IMT+p.I135T+p.I178IM+p.Y181YC+p.T200I	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IMT, I135T, I178IM, Y181YC, T200I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35K+p.T39A+p.M41L+p.K43E+p.V90VI+p.I142V+p.S162Y+p.E169D+p.V179I+p.M184V+p.Q197E+p.T215Y+p.V245VM	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35K, T39A, M41L, K43E, V90VI, I142V, S162Y, E169D, V179I, M184V, Q197E, T215Y, V245VM.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.E44D+p.K64R+p.V106I+p.T107S+p.V108I+p.L109I+p.K122E+p.D123NS+p.V179VI+p.M184V+p.G190A+p.T200A+p.I202V+p.E203K+p.H208Y+p.L210DE+p.R211K+p.T215Y+p.K223Q+p.A272P+p.L283I+p.R284K	Missense mutation	2.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, E44D, K64R, V106I, T107S, V108I, L109I, K122E, D123NS, V179VI, M184V, G190A, T200A, I202V, E203K, H208Y, L210DE, R211K, T215Y, K223Q, A272P, L283I, R284K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.T39A+p.R83K+p.A98APS+p.D123E+p.Q174HN+p.G196E+p.T200I+p.R211RK+p.V245VM+p.D250E+p.A272P+p.K277R+p.T286A+p.P294PS+p.E297R	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, T39A, R83K, A98APS, D123E, Q174HN, G196E, T200I, R211RK, V245VM, D250E, A272P, K277R, T286A, P294PS, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.D67N+p.T69D+p.K70R+p.V108VI+p.V118I+p.K122E+p.I135M+p.Y181C+p.M184V+p.G190GA+p.H208Y+p.R211K+p.F214L+p.T215F+p.D218E+p.K219Q+p.L228H+p.L260LV	Missense mutation	4.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, D67N, T69D, K70R, V108VI, V118I, K122E, I135M, Y181C, M184V, G190GA, H208Y, R211K, F214L, T215F, D218E, K219Q, L228H, L260LV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K101E+p.E138G+p.G190S+p.R211K+p.V245E+p.S251I	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K101E, E138G, G190S, R211K, V245E, S251I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K65R+p.K102Q+p.K103N+p.V106I+p.K122E+p.D123EK+p.D177E+p.Y188YC+p.G190GA+p.R211K+p.V245K+p.A272P+p.T286A+p.I293V	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K65R, K102Q, K103N, V106I, K122E, D123EK, D177E, Y188YC, G190GA, R211K, V245K, A272P, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.M41L+p.L74V+p.K103N+p.V118VI+p.K122E+p.I142V+p.A158S+p.E169D+p.F171Y+p.M184V+p.L210W+p.R211K+p.T215Y	Missense mutation	2.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, M41L, L74V, K103N, V118VI, K122E, I142V, A158S, E169D, F171Y, M184V, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39TA+p.I50IV+p.S68G+p.K122E+p.I135T+p.V179VD+p.G196E+p.R211RG+p.V245Q+p.A272P+p.K277KR+p.V292I	Missense mutation	1	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39TA, I50IV, S68G, K122E, I135T, V179VD, G196E, R211RG, V245Q, A272P, K277KR, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.T39TA+p.K43KT+p.A158S+p.G196GE+p.T200I+p.Q207E+p.K223KR	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, T39TA, K43KT, A158S, G196GE, T200I, Q207E, K223KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.V60I+p.K103N+p.K122E+p.D123DN+p.A158S+p.S162A+p.N175NY+p.T200A+p.Q207E+p.V245VE+p.T286A+p.I293IV	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, V60I, K103N, K122E, D123DN, A158S, S162A, N175NY, T200A, Q207E, V245VE, T286A, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.K103N+p.K166R+p.Q174H+p.T200I+p.I202V+p.Q207E+p.R211RK+p.I293V	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, K103N, K166R, Q174H, T200I, I202V, Q207E, R211RK, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.M41L+p.D67N+p.T69N+p.K70R+p.V75I+p.K101Q+p.I135T+p.Q174R+p.Y181C+p.M184V+p.G190S+p.T200A+p.L210F+p.R211K+p.T215Y+p.K219E+p.L228H	Missense mutation	2.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, M41L, D67N, T69N, K70R, V75I, K101Q, I135T, Q174R, Y181C, M184V, G190S, T200A, L210F, R211K, T215Y, K219E, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.T69S+p.K70R+p.K82R+p.R83K+p.K103N+p.I135L+p.I142T+p.S162A+p.I178IM+p.M184MV+p.Q207E+p.R211K+p.Q242QR+p.K277R+p.T286A	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, T69S, K70R, K82R, R83K, K103N, I135L, I142T, S162A, I178IM, M184MV, Q207E, R211K, Q242QR, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.D121Y+p.K122E+p.I135V+p.S162A+p.R172K+p.V179D+p.Y188L+p.Q207E+p.R211K+p.F214L+p.K277R+p.V292VI+p.I293V	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, D121Y, K122E, I135V, S162A, R172K, V179D, Y188L, Q207E, R211K, F214L, K277R, V292VI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.E36A+p.T39E+p.K43R+p.K122E+p.D123GS+p.K173X+p.D177E+p.I178M+p.T200V+p.Q207E+p.R211K+p.P243PT+p.V245EKQ+p.E248ED+p.D250DE+p.S251SC	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, E36A, T39E, K43R, K122E, D123GS, K173X, D177E, I178M, T200V, Q207E, R211K, P243PT, V245EKQ, E248ED, D250DE, S251SC.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.E36A+p.T39E+p.S48T+p.R83RG+p.K122E+p.D123S+p.E138A+p.A158S+p.T165TI+p.K173AE+p.D177E+p.T200A+p.I202V+p.Q207E	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, E36A, T39E, S48T, R83RG, K122E, D123S, E138A, A158S, T165TI, K173AE, D177E, T200A, I202V, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.I178IM+p.E204EA+p.Q207QE+p.R211RK+p.E248D+p.T286A	Missense mutation	1.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, I178IM, E204EA, Q207QE, R211RK, E248D, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.K49KR+p.V60I+p.K122KE+p.I135T+p.H198HY+p.I202IV+p.E204D+p.R211K	Missense mutation	1.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, K49KR, V60I, K122KE, I135T, H198HY, I202IV, E204D, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.K43KN+p.V60VI+p.K64KR+p.D67DN+p.W88C+p.A98AG+p.K103KN+p.K122E+p.D123E+p.I135V+p.S162A+p.I167M+p.K173T+p.Q174K+p.D177E+p.I178M+p.V179VM+p.Y181YC+p.M184V+p.T200TA+p.R206RK+p.Q207E+p.R211K+p.F214L+p.T215Y+p.K219KE+p.H221HY+p.L228LR	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, K43KN, V60VI, K64KR, D67DN, W88C, A98AG, K103KN, K122E, D123E, I135V, S162A, I167M, K173T, Q174K, D177E, I178M, V179VM, Y181YC, M184V, T200TA, R206RK, Q207E, R211K, F214L, T215Y, K219KE, H221HY, L228LR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41ML+p.K49KR+p.L74LI+p.D86E+p.W88C+p.K102KQ+p.K103R+p.K122KE+p.R172RK+p.K173N+p.Q174K+p.M184MV+p.G196E+p.T200TI+p.Q207E+p.L210LW+p.R211K+p.T215SY	Missense mutation	1.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41ML, K49KR, L74LI, D86E, W88C, K102KQ, K103R, K122KE, R172RK, K173N, Q174K, M184MV, G196E, T200TI, Q207E, L210LW, R211K, T215SY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.S48SA+p.V60VI+p.K122E+p.D123S+p.K173T+p.Q174K+p.D177E+p.I178M+p.T200E+p.Q207EG+p.R211K+p.F214FL+p.V245Q+p.E248N+p.D250DG+p.A272P+p.T286A+p.E291D+p.V292I+p.I293V+p.P294T	Missense mutation	0.5	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, S48SA, V60VI, K122E, D123S, K173T, Q174K, D177E, I178M, T200E, Q207EG, R211K, F214FL, V245Q, E248N, D250DG, A272P, T286A, E291D, V292I, I293V, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39EK+p.S48T+p.V60VI+p.D121H+p.K122E+p.I135T+p.S162SC+p.K173T+p.Q174K+p.D177E+p.I178L+p.M184V+p.T200A+p.Q207E+p.R211RK+p.V245Q+p.A272P+p.T286TA+p.E291D+p.V292I	Missense mutation	0.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39EK, S48T, V60VI, D121H, K122E, I135T, S162SC, K173T, Q174K, D177E, I178L, M184V, T200A, Q207E, R211RK, V245Q, A272P, T286TA, E291D, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39L+p.K103KN+p.D123E+p.G196E+p.Q207K+p.L210LM+p.R211A	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39L, K103KN, D123E, G196E, Q207K, L210LM, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39N+p.E40D+p.V60I+p.K104R+p.D123E+p.I135V+p.K173A+p.Q174K+p.T200A+p.Q207E+p.R211K+p.V245Q+p.A272P+p.K281R+p.E291D+p.V292I+p.I293V+p.E297A	Missense mutation	0.6	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39N, E40D, V60I, K104R, D123E, I135V, K173A, Q174K, T200A, Q207E, R211K, V245Q, A272P, K281R, E291D, V292I, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39TA+p.V60I+p.V90VI+p.K104KR+p.V106I+p.K122E+p.D123NS+p.K166R+p.K173T+p.Q174R+p.D177E+p.I178ILM+p.I195IV+p.T200A+p.Q207D+p.R211K+p.V245Q+p.E248D+p.D250E+p.A272P+p.K277R+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	0.6	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39TA, V60I, V90VI, K104KR, V106I, K122E, D123NS, K166R, K173T, Q174R, D177E, I178ILM, I195IV, T200A, Q207D, R211K, V245Q, E248D, D250E, A272P, K277R, T286A, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.A98AS+p.L109I+p.D121H+p.K122E+p.D123S+p.Q174E+p.D177E+p.I195T+p.T200A+p.V245Q+p.E248D+p.D250E+p.A272P+p.T286TA+p.E291D+p.V292I+p.I293V	Missense mutation	1.1	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, A98AS, L109I, D121H, K122E, D123S, Q174E, D177E, I195T, T200A, V245Q, E248D, D250E, A272P, T286TA, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.A98S+p.K122E+p.D123S+p.K173A+p.Q174K+p.D177E+p.I178M+p.T200A+p.Q207N+p.R211K+p.V245Q+p.E248D+p.D250E+p.A272P+p.T286TA+p.E291D+p.V292I+p.I293V	Missense mutation	0.5	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, A98S, K122E, D123S, K173A, Q174K, D177E, I178M, T200A, Q207N, R211K, V245Q, E248D, D250E, A272P, T286TA, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.D123E+p.K173T+p.Q174QK+p.D177E+p.T200A+p.I202V+p.Q207D+p.V245Q+p.E248D+p.D250E+p.A272P+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	0.6	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, D123E, K173T, Q174QK, D177E, T200A, I202V, Q207D, V245Q, E248D, D250E, A272P, T286A, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.D67DN+p.R83RK+p.K122I+p.D123E+p.M184V+p.T200A+p.Q207E+p.V245Q+p.E248N+p.T286A	Missense mutation	0.6	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, D67DN, R83RK, K122I, D123E, M184V, T200A, Q207E, V245Q, E248N, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K101R+p.K122E+p.I135V+p.I142V+p.T200TA+p.Q207QE+p.R211K	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K101R, K122E, I135V, I142V, T200TA, Q207QE, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K103N+p.K122KE+p.I135V+p.Q207E+p.R211K+p.V245E+p.L283I+p.I293V	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K103N, K122KE, I135V, Q207E, R211K, V245E, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K122Q+p.D123S+p.S162C+p.K166R+p.K173T+p.Q174R+p.D177E+p.I178L+p.T200A+p.Q207D+p.R211K+p.P243PS+p.V245K+p.E248D+p.A272P+p.K277R+p.T286A+p.E291D+p.V292I+p.I293V	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K122Q, D123S, S162C, K166R, K173T, Q174R, D177E, I178L, T200A, Q207D, R211K, P243PS, V245K, E248D, A272P, K277R, T286A, E291D, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.S68G+p.I135R+p.S162A+p.K173T+p.Q174R+p.D177E+p.I178L+p.T200A+p.Q207D+p.R211K+p.V245Q+p.E248D+p.D250E+p.A272P+p.T286A+p.E291D+p.V292I+p.I293V+p.P294T+p.E297A	Missense mutation	0.7	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, S68G, I135R, S162A, K173T, Q174R, D177E, I178L, T200A, Q207D, R211K, V245Q, E248D, D250E, A272P, T286A, E291D, V292I, I293V, P294T, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V90VI+p.K103N+p.D121H+p.K122E+p.I135IV+p.P170PT+p.Q174E+p.Y181YC+p.Q197QR+p.T200A+p.Q207E+p.L228LR+p.V245MT+p.E248N+p.A272P+p.T286TA	Missense mutation	1.9	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V90VI, K103N, D121H, K122E, I135IV, P170PT, Q174E, Y181YC, Q197QR, T200A, Q207E, L228LR, V245MT, E248N, A272P, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VA+p.D67N+p.K70R+p.K103N+p.K122E+p.K173Q+p.M184V+p.E203EA+p.K219Q+p.E248D+p.A272P+p.K281R+p.T286A+p.I293IV+p.P294T+p.E297R	Missense mutation	3.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VA, D67N, K70R, K103N, K122E, K173Q, M184V, E203EA, K219Q, E248D, A272P, K281R, T286A, I293IV, P294T, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VA+p.K101KQ+p.K122E+p.R211RK	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VA, K101KQ, K122E, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VA+p.S48T+p.V60I+p.K122E+p.I135V+p.T139TS+p.I142IT+p.S162X+p.K166R+p.Q174HN+p.T200TA+p.I202IV+p.R211RK	Missense mutation	1.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VA, S48T, V60I, K122E, I135V, T139TS, I142IT, S162X, K166R, Q174HN, T200TA, I202IV, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VA+p.V60VI+p.V90VI+p.K103N+p.V108VI+p.K122E+p.D123DN+p.I135T+p.S162X+p.M184V+p.T200A+p.A272P	Missense mutation	1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VA, V60VI, V90VI, K103N, V108VI, K122E, D123DN, I135T, S162X, M184V, T200A, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.D67N+p.K70G+p.A98AG+p.D123E+p.D177E+p.M184MV+p.T200TA+p.E203K+p.R211K+p.K219Q	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, D67N, K70G, A98AG, D123E, D177E, M184MV, T200TA, E203K, R211K, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.K43KE+p.W88WC+p.K122E+p.I178M+p.T200TA+p.E204ED+p.L205LP+p.R211RK+p.L228LP+p.S251T+p.T286A+p.A288G+p.T296S	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, K43KE, W88WC, K122E, I178M, T200TA, E204ED, L205LP, R211RK, L228LP, S251T, T286A, A288G, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.K65R+p.S68N+p.T69S+p.V90I+p.K101KR+p.K103N+p.V106I+p.D177E+p.V179VL+p.Y181C+p.Q207KR+p.R211Q+p.H221Y+p.M230L+p.A272P+p.V292I+p.I293V+p.P294S+p.E297K	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, K65R, S68N, T69S, V90I, K101KR, K103N, V106I, D177E, V179VL, Y181C, Q207KR, R211Q, H221Y, M230L, A272P, V292I, I293V, P294S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.M41L+p.V60I+p.D67N+p.K70R+p.L74I+p.V75T+p.L100I+p.K103N+p.K122E+p.I135V+p.V179IT+p.M184MV+p.T200A+p.I202V+p.E203K+p.R206RK+p.Q207E+p.T215F+p.K219E+p.L228H+p.D237DN	Missense mutation	3.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, M41L, V60I, D67N, K70R, L74I, V75T, L100I, K103N, K122E, I135V, V179IT, M184MV, T200A, I202V, E203K, R206RK, Q207E, T215F, K219E, L228H, D237DN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.P119PL+p.D123E+p.Q174K+p.I178M+p.V179VE+p.G196E+p.T200A+p.Q207X+p.R211A	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, P119PL, D123E, Q174K, I178M, V179VE, G196E, T200A, Q207X, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.T39TA+p.V60I+p.K103N+p.V106VI+p.K122E+p.D123S+p.T139TA+p.G196E+p.R211RK	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, T39TA, V60I, K103N, V106VI, K122E, D123S, T139TA, G196E, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.T39TS+p.R83RK+p.K103N+p.D123E+p.S162A+p.K173X+p.T200A+p.F214L+p.V245VI+p.D250DE	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, T39TS, R83RK, K103N, D123E, S162A, K173X, T200A, F214L, V245VI, D250DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.V179VI+p.Q197E+p.R211K+p.F214L+p.A272P+p.I293IV	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, V179VI, Q197E, R211K, F214L, A272P, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.Y56YC+p.G196E	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, Y56YC, G196E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.E36D+p.T39TI+p.E40ED+p.V60I+p.K102KR+p.K104KR+p.I135V+p.S162A+p.K173T+p.Q174K+p.D177E+p.Q197L+p.T200A+p.Q207E+p.R211RK+p.V245K+p.T286A+p.E291D+p.V292I+p.I293V+p.P294IV	Missense mutation	0.7	Susceptible	[29]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, E36D, T39TI, E40ED, V60I, K102KR, K104KR, I135V, S162A, K173T, Q174K, D177E, Q197L, T200A, Q207E, R211RK, V245K, T286A, E291D, V292I, I293V, P294IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.E53ED+p.A158AS+p.R211K+p.K238KR	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, E53ED, A158AS, R211K, K238KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.T39TK+p.K49KR+p.V60VI+p.R83K+p.D123DE+p.D177DE+p.V245M+p.D250E+p.S251DN+p.A272P+p.V292I+p.I293V	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, T39TK, K49KR, V60VI, R83K, D123DE, D177DE, V245M, D250E, S251DN, A272P, V292I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D67N+p.T69N+p.K103N+p.D121Y+p.K122E+p.I142V+p.S162N+p.D177E+p.V179T+p.Y188L+p.F214L+p.T215L+p.P217YF+p.K219Q+p.L228H+p.K238Q+p.A272AP	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D67N, T69N, K103N, D121Y, K122E, I142V, S162N, D177E, V179T, Y188L, F214L, T215L, P217YF, K219Q, L228H, K238Q, A272AP.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D67N+p.V90I+p.T139I+p.I142T+p.I195M+p.G196E+p.T200A+p.K219Q	Missense mutation	1.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D67N, V90I, T139I, I142T, I195M, G196E, T200A, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.K103KN+p.I142V+p.P243PS+p.V245X+p.E248X+p.D250DN+p.T286A	Missense mutation	0.9	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, K103KN, I142V, P243PS, V245X, E248X, D250DN, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.K103N+p.K122E+p.D123E+p.I142V+p.G196E+p.A272P+p.K281R+p.T286A+p.P294A+p.E297K	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, K103N, K122E, D123E, I142V, G196E, A272P, K281R, T286A, P294A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.K104KR+p.E138EA+p.I142IMT+p.R211K+p.F214FL	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, K104KR, E138EA, I142IMT, R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.K122E+p.D123E+p.I135T+p.I142V+p.M184V+p.G196E+p.K201KR+p.Q207E+p.R211K+p.D237DN	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, K122E, D123E, I135T, I142V, M184V, G196E, K201KR, Q207E, R211K, D237DN.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.R83K+p.K122E+p.D123E+p.I135V+p.D177DE+p.I178IM+p.I202IV+p.R211K	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, R83K, K122E, D123E, I135V, D177DE, I178IM, I202IV, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.S68G+p.K70Q+p.K103KN+p.K122E+p.I142V+p.Q174K+p.I178L+p.M184V+p.T200K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	1.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, S68G, K70Q, K103KN, K122E, I142V, Q174K, I178L, M184V, T200K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.T69TN+p.K101R+p.K122KE+p.I135T+p.Y181YC+p.M184MV+p.G190GA+p.T200TI+p.V245M+p.A272P+p.A288AS	Missense mutation	0.6	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, T69TN, K101R, K122KE, I135T, Y181YC, M184MV, G190GA, T200TI, V245M, A272P, A288AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60L+p.D123E+p.I142V+p.I178L+p.G196E+p.R211K+p.F214FL	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60L, D123E, I142V, I178L, G196E, R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8I+p.V35T+p.K43KR+p.E44D+p.K49R+p.V60I+p.D121Y+p.K122E+p.K166KQ+p.D177E+p.I178M+p.T200L+p.Q207E+p.R211K+p.V245H+p.D250E+p.A272P+p.K277R+p.L282C+p.L283I+p.I293V+p.G335D+p.P345Q+p.F346Y	Missense mutation	0.6	Susceptible	[32]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8I, V35T, K43KR, E44D, K49R, V60I, D121Y, K122E, K166KQ, D177E, I178M, T200L, Q207E, R211K, V245H, D250E, A272P, K277R, L282C, L283I, I293V, G335D, P345Q, F346Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8I+p.V35T+p.K49R+p.K122E+p.I135T+p.E169D+p.T200TI+p.I202V+p.R211K	Missense mutation	1.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8I, V35T, K49R, K122E, I135T, E169D, T200TI, I202V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K101KQ+p.K103N+p.K122KE+p.I135T+p.E138EG+p.Q207R+p.R211RK+p.F214L+p.K238T	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K101KQ, K103N, K122KE, I135T, E138EG, Q207R, R211RK, F214L, K238T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K103KN+p.K104KR+p.T139TIM+p.I142IT+p.I178IV+p.R211RK	Missense mutation	2.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K103KN, K104KR, T139TIM, I142IT, I178IV, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.K20R+p.I31IV+p.T39A+p.M41L+p.E44ED+p.D67G+p.S68G+p.L74V+p.L100I+p.K103N+p.K122E+p.D123DN+p.K166KR+p.M184V+p.T200A+p.H208Y+p.R211K+p.T215Y+p.K219N+p.H221HY+p.L228H	Missense mutation	0.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, K20R, I31IV, T39A, M41L, E44ED, D67G, S68G, L74V, L100I, K103N, K122E, D123DN, K166KR, M184V, T200A, H208Y, R211K, T215Y, K219N, H221HY, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.V35VI+p.K122KE+p.I202IV+p.R211RK	Missense mutation	0.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, V35VI, K122KE, I202IV, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.W88C+p.H96HN+p.A98S+p.K122E+p.T131TS+p.E138A+p.I202IV+p.R211K	Missense mutation	0.8	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included W88C, H96HN, A98S, K122E, T131TS, E138A, I202IV, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K101R+p.K103N+p.S162A+p.Y181C+p.Q207E+p.D218E+p.K219Q+p.L228R+p.P243T+p.E248D+p.A272P+p.T286A+p.I293V+p.E297K	Missense mutation	7.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K101R, K103N, S162A, Y181C, Q207E, D218E, K219Q, L228R, P243T, E248D, A272P, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.K101R+p.K103N+p.S162A+p.Y181C+p.Q207E+p.R211RK+p.T215X+p.D218E+p.K219Q+p.L228LR+p.P243T+p.E248D+p.A272P+p.T286A+p.I293V+p.E297K	Missense mutation	5.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, K101R, K103N, S162A, Y181C, Q207E, R211RK, T215X, D218E, K219Q, L228LR, P243T, E248D, A272P, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.K32E+p.V35T+p.V60I+p.D67N+p.T69D+p.K70R+p.K101E+p.F116Y+p.V118I+p.K122E+p.D123E+p.Q151M+p.V179F+p.Y181C+p.M184V+p.F214L+p.T215V+p.K219Q+p.L228H+p.T286A+p.I293V	Missense mutation	9.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, K32E, V35T, V60I, D67N, T69D, K70R, K101E, F116Y, V118I, K122E, D123E, Q151M, V179F, Y181C, M184V, F214L, T215V, K219Q, L228H, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.V35M+p.K43Q+p.E44D+p.A62V+p.K101P+p.K122E+p.V179L+p.M184V+p.T200A+p.E203D+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H	Missense mutation	6.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, V35M, K43Q, E44D, A62V, K101P, K122E, V179L, M184V, T200A, E203D, Q207E, L210W, R211K, T215Y, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40EK+p.D67DN+p.V106I+p.K122KE+p.F130FL+p.T131TP+p.E138EV+p.T139TRS+p.Y188L+p.I202V+p.H208Y+p.R211RK+p.T215Y	Missense mutation	7.2	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40EK, D67DN, V106I, K122KE, F130FL, T131TP, E138EV, T139TRS, Y188L, I202V, H208Y, R211RK, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40EK+p.M41L+p.K43EQ+p.E44D+p.D67N+p.T69D+p.R83K+p.A98G+p.K103KN+p.V118I+p.K122E+p.D123DN+p.S162HY+p.V179I+p.M184V+p.T200I+p.L210W+p.T215Y+p.D218E+p.K219KR	Missense mutation	7	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40EK, M41L, K43EQ, E44D, D67N, T69D, R83K, A98G, K103KN, V118I, K122E, D123DN, S162HY, V179I, M184V, T200I, L210W, T215Y, D218E, K219KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40X+p.A62V+p.D67N+p.V75I+p.W88WS+p.K103KN+p.F116FY+p.K122E+p.D123E+p.P150PA+p.Q151X+p.D177E+p.L187LF+p.G190GA+p.Q207E+p.H208Y+p.R211K+p.T215NY+p.D218E+p.K219Q+p.H235HY	Missense mutation	6.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40X, A62V, D67N, V75I, W88WS, K103KN, F116FY, K122E, D123E, P150PA, Q151X, D177E, L187LF, G190GA, Q207E, H208Y, R211K, T215NY, D218E, K219Q, H235HY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.D67DN+p.R83K+p.K103N+p.A158AT+p.D177E+p.V179VI+p.Y181C+p.T200A+p.E203K+p.Q207E+p.R211K+p.T215Y+p.L228LR+p.A272P+p.T286TA+p.E297K	Missense mutation	6.9	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, D67DN, R83K, K103N, A158AT, D177E, V179VI, Y181C, T200A, E203K, Q207E, R211K, T215Y, L228LR, A272P, T286TA, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T39TA+p.M41L+p.V60I+p.D67N+p.K70R+p.L74LI+p.K101KQ+p.K102KQ+p.K103N+p.V108I+p.I135IV+p.D177DN+p.M184V+p.L210LW+p.R211T+p.T215Y+p.K219E+p.L228H	Missense mutation	4.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T39TA, M41L, V60I, D67N, K70R, L74LI, K101KQ, K102KQ, K103N, V108I, I135IV, D177DN, M184V, L210LW, R211T, T215Y, K219E, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.V35VI+p.M41L+p.D67N+p.R83K+p.D123E+p.I178L+p.M184V+p.I202V+p.Q207E+p.L210W+p.R211RK+p.T215Y	Missense mutation	8.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, V35VI, M41L, D67N, R83K, D123E, I178L, M184V, I202V, Q207E, L210W, R211RK, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.V35VIM+p.E36EQK+p.T39TA+p.E40EK+p.M41L+p.K43E+p.K64R+p.D67N+p.L74I+p.V75T+p.K103N+p.V118I+p.K122E+p.M184MV+p.G196E+p.T200A+p.E203K+p.Q207E+p.L210W+p.T215Y+p.D218E+p.K219D+p.F227Y+p.L228H+p.L234I+p.A272AP+p.T286A	Missense mutation	9.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, V35VIM, E36EQK, T39TA, E40EK, M41L, K43E, K64R, D67N, L74I, V75T, K103N, V118I, K122E, M184MV, G196E, T200A, E203K, Q207E, L210W, T215Y, D218E, K219D, F227Y, L228H, L234I, A272AP, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6G+p.M41L+p.D67N+p.R83K+p.W88G+p.D113E+p.I135T+p.K173E+p.Y181C+p.G196E+p.T200I+p.T215N+p.K219E+p.H221Y	Missense mutation	9.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6G, M41L, D67N, R83K, W88G, D113E, I135T, K173E, Y181C, G196E, T200I, T215N, K219E, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31IV+p.M41L+p.T69D+p.V90VI+p.K122KE+p.S162AD+p.Y181C+p.Q207E+p.L210W+p.R211K+p.T215CY+p.L228R	Missense mutation	5.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31IV, M41L, T69D, V90VI, K122KE, S162AD, Y181C, Q207E, L210W, R211K, T215CY, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.D67HN+p.T69TN+p.K70KR+p.W88C+p.V118VI+p.A158S+p.S162D+p.K166R+p.E169D+p.M184V+p.R211K+p.F214L+p.T215FV+p.K219E+p.V245M+p.E297K	Missense mutation	4.3	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, D67HN, T69TN, K70KR, W88C, V118VI, A158S, S162D, K166R, E169D, M184V, R211K, F214L, T215FV, K219E, V245M, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V21I+p.V35I+p.E40F+p.M41L+p.K43E+p.E53EK+p.V60VI+p.D67N+p.L74LI+p.K101E+p.V118I+p.K122E+p.D123N+p.R172RK+p.Q174QR+p.Y181C+p.G190A+p.L210W+p.R211RK+p.F214FL+p.T215Y+p.K219N+p.V245E+p.D250DE+p.K277KR+p.R284RK	Missense mutation	5.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V21I, V35I, E40F, M41L, K43E, E53EK, V60VI, D67N, L74LI, K101E, V118I, K122E, D123N, R172RK, Q174QR, Y181C, G190A, L210W, R211RK, F214FL, T215Y, K219N, V245E, D250DE, K277KR, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35I+p.T39A+p.M41L+p.E44D+p.V60I+p.D67N+p.K70R+p.L74LI+p.R83RK+p.K103N+p.V108I+p.I135T+p.D177N+p.M184V+p.G196E+p.T200TI+p.E203K+p.H208Y+p.R211K+p.T215Y+p.K219E+p.K223T+p.L228H+p.L234I	Missense mutation	7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35I, T39A, M41L, E44D, V60I, D67N, K70R, L74LI, R83RK, K103N, V108I, I135T, D177N, M184V, G196E, T200TI, E203K, H208Y, R211K, T215Y, K219E, K223T, L228H, L234I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35VI+p.D67N+p.T69D+p.K70R+p.D121DY+p.K122KE+p.D123DE+p.I135T+p.D177E+p.M184V+p.T200TA+p.T215Y+p.D218E+p.K219Q	Missense mutation	9.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35VI, D67N, T69D, K70R, D121DY, K122KE, D123DE, I135T, D177E, M184V, T200TA, T215Y, D218E, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.D67N+p.T69N+p.K70R+p.L74V+p.K103N+p.K122EG+p.I135T+p.Y181C+p.M184V+p.Q207R+p.F214L+p.T215F+p.D218E+p.K219Q+p.Q278H+p.T286A	Missense mutation	6.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, D67N, T69N, K70R, L74V, K103N, K122EG, I135T, Y181C, M184V, Q207R, F214L, T215F, D218E, K219Q, Q278H, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K22KE+p.M41L+p.K43Q+p.E44D+p.D67DN+p.L74LV+p.K103KN+p.V118I+p.K122E+p.M184V+p.H208HY+p.L210LW+p.R211RK+p.F214FL+p.T215Y+p.K219KN+p.K223KQ+p.D237DE+p.V245E+p.A272P+p.K281R+p.R284K+p.T286A+p.E291D+p.E297K	Missense mutation	4.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K22KE, M41L, K43Q, E44D, D67DN, L74LV, K103KN, V118I, K122E, M184V, H208HY, L210LW, R211RK, F214FL, T215Y, K219KN, K223KQ, D237DE, V245E, A272P, K281R, R284K, T286A, E291D, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K43E+p.P59PL+p.D67N+p.L74V+p.K101E+p.K102KR+p.K104KR+p.V118I+p.K122KE+p.I135L+p.Q161QR+p.K173N+p.Y181V+p.T200A+p.I202V+p.E204EK+p.Q207QK+p.L210W+p.R211K+p.T215FY+p.V245X+p.S268SG+p.A272P+p.T296S+p.E297K	Missense mutation	8.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K43E, P59PL, D67N, L74V, K101E, K102KR, K104KR, V118I, K122KE, I135L, Q161QR, K173N, Y181V, T200A, I202V, E204EK, Q207QK, L210W, R211K, T215FY, V245X, S268SG, A272P, T296S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.V60I+p.D67N+p.K70GRS+p.K103KN+p.K122P+p.D177E+p.M184V+p.G196E+p.T215F+p.D218E+p.K219Q+p.V245T+p.K277R+p.L283LI+p.T286A+p.A288S+p.I293V+p.P294T+p.E297K	Missense mutation	6.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, V60I, D67N, K70GRS, K103KN, K122P, D177E, M184V, G196E, T215F, D218E, K219Q, V245T, K277R, L283LI, T286A, A288S, I293V, P294T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.T69D+p.A98S+p.V118I+p.K122E+p.D123E+p.I135V+p.S162C+p.M184V+p.T200A+p.E203K+p.L210W+p.R211RK+p.T215Y+p.K223E+p.E224D+p.L228R+p.V245E+p.A272P+p.T286A+p.I293V+p.E297K	Missense mutation	5.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, T69D, A98S, V118I, K122E, D123E, I135V, S162C, M184V, T200A, E203K, L210W, R211RK, T215Y, K223E, E224D, L228R, V245E, A272P, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35IM+p.M41L+p.K43N+p.E44D+p.D67N+p.T69D+p.L100LR+p.V118I+p.D123E+p.V179I+p.M184V+p.T200X+p.E203D+p.Q207E+p.H208FY+p.L210W+p.R211K+p.T215Y	Missense mutation	8.1	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35IM, M41L, K43N, E44D, D67N, T69D, L100LR, V118I, D123E, V179I, M184V, T200X, E203D, Q207E, H208FY, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.M41L+p.E44ED+p.K64KR+p.D67N+p.L74I+p.K103N+p.V106M+p.V111I+p.E169D+p.Q174QR+p.V179I+p.M184V+p.T200A+p.I202IV+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219R+p.K223Q+p.L228Q	Missense mutation	9.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, M41L, E44ED, K64KR, D67N, L74I, K103N, V106M, V111I, E169D, Q174QR, V179I, M184V, T200A, I202IV, H208Y, L210W, R211K, T215Y, K219R, K223Q, L228Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VL+p.T39A+p.M41L+p.E44D+p.V60I+p.D67N+p.T69D+p.V75M+p.F77FL+p.K101P+p.K102Q+p.V111I+p.V118I+p.K122E+p.I135V+p.K173E+p.V179I+p.M184V+p.G190A+p.H208HY+p.L210W+p.T215Y+p.D218E+p.K219Q+p.L228K+p.A272S+p.K277R+p.Q278E+p.L283I+p.A288S+p.E297K	Missense mutation	7.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VL, T39A, M41L, E44D, V60I, D67N, T69D, V75M, F77FL, K101P, K102Q, V111I, V118I, K122E, I135V, K173E, V179I, M184V, G190A, H208HY, L210W, T215Y, D218E, K219Q, L228K, A272S, K277R, Q278E, L283I, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.D67N+p.T69D+p.K70R+p.A98G+p.K103KN+p.K122E+p.D123E+p.I135T+p.M184V+p.T200V+p.E203EQ+p.Q207D+p.R211RK+p.F214LM+p.T215TI+p.K219Q+p.P225H+p.L228H+p.Q242L+p.I244IV+p.A272P+p.L282I+p.E291D	Missense mutation	8.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, D67N, T69D, K70R, A98G, K103KN, K122E, D123E, I135T, M184V, T200V, E203EQ, Q207D, R211RK, F214LM, T215TI, K219Q, P225H, L228H, Q242L, I244IV, A272P, L282I, E291D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.T69N+p.K70R+p.K103N+p.D121Y+p.K122E+p.K166R+p.D177E+p.I178IM+p.V179VI+p.Y181C+p.R211K+p.F214L+p.K219Q+p.L228R	Missense mutation	7.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, T69N, K70R, K103N, D121Y, K122E, K166R, D177E, I178IM, V179VI, Y181C, R211K, F214L, K219Q, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22KR+p.D67N+p.T69N+p.K70R+p.L74I+p.D86E+p.W88C+p.K103N+p.V118I+p.K122E+p.I135V+p.T165L+p.M184V+p.G196E+p.T200TA+p.F214L+p.T215F+p.K219Q+p.V245E+p.K275R+p.I293IV+p.E297K	Missense mutation	9.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22KR, D67N, T69N, K70R, L74I, D86E, W88C, K103N, V118I, K122E, I135V, T165L, M184V, G196E, T200TA, F214L, T215F, K219Q, V245E, K275R, I293IV, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32KR+p.V35T+p.K49R+p.V60I+p.S68SG+p.K70KN+p.V90I+p.I94IM+p.K103N+p.D121DG+p.K122E+p.E138Q+p.R172RK+p.D177E+p.Q207D+p.H208Y+p.R211K+p.T215F+p.K219Q+p.L228H+p.M230L	Missense mutation	7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32KR, V35T, K49R, V60I, S68SG, K70KN, V90I, I94IM, K103N, D121DG, K122E, E138Q, R172RK, D177E, Q207D, H208Y, R211K, T215F, K219Q, L228H, M230L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KE+p.K49R+p.I50V+p.V60I+p.D67DN+p.K70KR+p.A98AS+p.I135IV+p.S162SN+p.M184MV+p.Q207EK+p.K219KQ	Missense mutation	4.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KE, K49R, I50V, V60I, D67DN, K70KR, A98AS, I135IV, S162SN, M184MV, Q207EK, K219KQ.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KR+p.K49KR+p.V60I+p.D67DN+p.T69TN+p.K70KR+p.R83RK+p.K102KQ+p.K122KE+p.D123DE+p.I135T+p.I178IM+p.V179VI+p.G190GA+p.T200TA+p.T215X+p.K219KE+p.K223KQ+p.L228LH+p.V245E+p.A272P+p.V276I+p.L283I+p.I293V	Missense mutation	9.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KR, K49KR, V60I, D67DN, T69TN, K70KR, R83RK, K102KQ, K122KE, D123DE, I135T, I178IM, V179VI, G190GA, T200TA, T215X, K219KE, K223KQ, L228LH, V245E, A272P, V276I, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.I50V+p.K103KN+p.K122E+p.D123DG+p.I135LV+p.Q207QK+p.R211K+p.T215Y+p.D237DY	Missense mutation	6.5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, I50V, K103KN, K122E, D123DG, I135LV, Q207QK, R211K, T215Y, D237DY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.L74IV+p.K102KR+p.K122E+p.D123DN+p.I135V+p.V179T+p.Y181C+p.M184V+p.I202V+p.T215F+p.D218E+p.K219Q+p.L228H+p.A272AP+p.A288S	Missense mutation	4.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, L74IV, K102KR, K122E, D123DN, I135V, V179T, Y181C, M184V, I202V, T215F, D218E, K219Q, L228H, A272AP, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.T69D+p.K101Q+p.V118I+p.K122E+p.D123E+p.V179I+p.M184V+p.H208Y+p.L210W+p.T215F+p.K277R+p.V292VI+p.I293V	Missense mutation	4.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, T69D, K101Q, V118I, K122E, D123E, V179I, M184V, H208Y, L210W, T215F, K277R, V292VI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122E+p.D123E+p.I142V+p.K166R+p.M184V+p.Q207E+p.R211K+p.T215Y+p.V245E+p.A272P+p.K281R+p.E297K	Missense mutation	4.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122E, D123E, I142V, K166R, M184V, Q207E, R211K, T215Y, V245E, A272P, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122KE+p.I142V+p.K166IT+p.I178IM+p.R211KT	Missense mutation	8.4	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122KE, I142V, K166IT, I178IM, R211KT.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43E+p.D67N+p.L74V+p.K101KE+p.V118I+p.K122P+p.F130FL+p.T131TP+p.S162C+p.I178IM+p.Y181C+p.G190A+p.G196E+p.E203V+p.L210W+p.T215Y+p.D218DN+p.K219N	Missense mutation	9.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43E, D67N, L74V, K101KE, V118I, K122P, F130FL, T131TP, S162C, I178IM, Y181C, G190A, G196E, E203V, L210W, T215Y, D218DN, K219N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43KQ+p.D67DN+p.R83K+p.H96HN+p.V118I+p.L120L*+p.N137NI+p.G152GE+p.D177E+p.M184V+p.T200A+p.E203EK+p.Q207EG+p.H208HY+p.L210W+p.R211K+p.T215Y	Missense mutation	5.7	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43KQ, D67DN, R83K, H96HN, V118I, L120L*, N137NI, G152GE, D177E, M184V, T200A, E203EK, Q207EG, H208HY, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.H96HN+p.K101Q+p.V111VL+p.D113DN+p.V118I+p.D121C+p.K122E+p.T131TS+p.D177E+p.V179I+p.Y181C+p.M184V+p.G196R+p.T200I+p.L210W+p.R211K+p.T215Y+p.K219S+p.L228R+p.V245K	Missense mutation	5.4	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, H96HN, K101Q, V111VL, D113DN, V118I, D121C, K122E, T131TS, D177E, V179I, Y181C, M184V, G196R, T200I, L210W, R211K, T215Y, K219S, L228R, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74I+p.K103N+p.V118I+p.K122E+p.D177N+p.I178M+p.M184V+p.T200A+p.L210W+p.T215Y+p.K277R	Missense mutation	7.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74I, K103N, V118I, K122E, D177N, I178M, M184V, T200A, L210W, T215Y, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.M184V+p.T215Y	Missense mutation	6.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, M184V, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.M184V+p.Y188L+p.T215Y	Missense mutation	6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, M184V, Y188L, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68G+p.K103N+p.D123E+p.I135T+p.D177E+p.I178L+p.M184V+p.E194D+p.G196E+p.T200TI+p.T215Y	Missense mutation	7.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68G, K103N, D123E, I135T, D177E, I178L, M184V, E194D, G196E, T200TI, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68N+p.L74V+p.K103N+p.K122E+p.Q174R+p.M184V+p.V189VI+p.G196E+p.L210W+p.R211K+p.T215Y+p.A272P+p.E297R	Missense mutation	6.3	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68N, L74V, K103N, K122E, Q174R, M184V, V189VI, G196E, L210W, R211K, T215Y, A272P, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68SG+p.L74LI+p.K122E+p.D123DN+p.I135IV+p.I142IV+p.I178L+p.M184V+p.G196GE+p.T200A+p.E204D+p.L210LW+p.R211RT+p.T215Y+p.L228LH+p.R284RK	Missense mutation	4.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68SG, L74LI, K122E, D123DN, I135IV, I142IV, I178L, M184V, G196GE, T200A, E204D, L210LW, R211RT, T215Y, L228LH, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41ML+p.D67DN+p.K70X+p.I135T+p.S162SC+p.M184MV+p.E194EA+p.G196GE+p.T200S+p.I202V+p.T215X+p.K219KE+p.A272P+p.K275Q+p.V276X+p.K277KR+p.E291ED+p.V292I	Missense mutation	7.8	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41ML, D67DN, K70X, I135T, S162SC, M184MV, E194EA, G196GE, T200S, I202V, T215X, K219KE, A272P, K275Q, V276X, K277KR, E291ED, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1H+p.P4S+p.V21F+p.D67N+p.T69D+p.K70R+p.V90I+p.D123Q+p.I135T+p.T139TK+p.S162Y+p.D177E+p.I178IM+p.V179I+p.Y181C+p.M184I+p.Y188L+p.G190A+p.T200A+p.R211K+p.K219Q	Missense mutation	5.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1H, P4S, V21F, D67N, T69D, K70R, V90I, D123Q, I135T, T139TK, S162Y, D177E, I178IM, V179I, Y181C, M184I, Y188L, G190A, T200A, R211K, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1H+p.P4S+p.W24WG+p.D67N+p.K70R+p.V90I+p.K122E+p.D123Q+p.I135T+p.S162H+p.D177E+p.I178IM+p.V179I+p.Y181C+p.M184I+p.Y188L+p.G190A+p.T200A+p.R211K+p.F214FL+p.T215F+p.K219Q+p.E224D+p.V245K+p.K277KR+p.Q278E+p.V292VI+p.E297R	Missense mutation	5.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1H, P4S, W24WG, D67N, K70R, V90I, K122E, D123Q, I135T, S162H, D177E, I178IM, V179I, Y181C, M184I, Y188L, G190A, T200A, R211K, F214FL, T215F, K219Q, E224D, V245K, K277KR, Q278E, V292VI, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.E6D+p.K20R+p.M41L+p.E44ED+p.L74V+p.V75VA+p.K103N+p.K122E+p.D123DN+p.I135M+p.Y181C+p.V189I+p.Q197K+p.T200TA+p.Q207E+p.L210W+p.T215Y+p.L228R	Missense mutation	8.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, E6D, K20R, M41L, E44ED, L74V, V75VA, K103N, K122E, D123DN, I135M, Y181C, V189I, Q197K, T200TA, Q207E, L210W, T215Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.K20R+p.A62V+p.D67N+p.S68N+p.K70G+p.K101Q+p.K103N+p.F116Y+p.K122E+p.I135T+p.Q151M+p.V179I+p.M184I+p.I202V+p.L210LW+p.R211K+p.T215Y+p.T286A+p.E297K	Missense mutation	4.5	Susceptible	[5]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, K20R, A62V, D67N, S68N, K70G, K101Q, K103N, F116Y, K122E, I135T, Q151M, V179I, M184I, I202V, L210LW, R211K, T215Y, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1PL+p.V35M+p.M41L+p.K43N+p.E44D+p.E53D+p.D67N+p.K70KE+p.K103N+p.V118VI+p.D121Y+p.K122E+p.S162T+p.M184V+p.T200A+p.E203D+p.R211K+p.T215Y+p.K219KR+p.H221HY+p.L234LP	Missense mutation	9.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1PL, V35M, M41L, K43N, E44D, E53D, D67N, K70KE, K103N, V118VI, D121Y, K122E, S162T, M184V, T200A, E203D, R211K, T215Y, K219KR, H221HY, L234LP.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.K20R+p.M41L+p.V60I+p.D67N+p.K70R+p.V118VI+p.I135T+p.M184V+p.T200IL+p.T215Y+p.K219E+p.P225H+p.L228H	Missense mutation	7.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, K20R, M41L, V60I, D67N, K70R, V118VI, I135T, M184V, T200IL, T215Y, K219E, P225H, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.M41L+p.D67DN+p.A98S+p.V118I+p.K122E+p.D123E+p.I135V+p.K166R+p.M184V+p.L210W+p.R211K+p.T215Y+p.K219KN+p.I244IV+p.A272P+p.K277R+p.T286A+p.V292I+p.I293V+p.E297Q	Missense mutation	9.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, M41L, D67DN, A98S, V118I, K122E, D123E, I135V, K166R, M184V, L210W, R211K, T215Y, K219KN, I244IV, A272P, K277R, T286A, V292I, I293V, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.M41L+p.L74LV+p.A98S+p.V118I+p.K122E+p.D123E+p.I135V+p.K166R+p.M184V+p.L210W+p.R211K+p.T215Y+p.A272P+p.K277R+p.T286A+p.V292I+p.I293V+p.E297Q	Missense mutation	5.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, M41L, L74LV, A98S, V118I, K122E, D123E, I135V, K166R, M184V, L210W, R211K, T215Y, A272P, K277R, T286A, V292I, I293V, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.T39TA+p.M41L+p.K43KQ+p.V60I+p.D67N+p.T69X+p.K70KR+p.K101Q+p.K122E+p.I135V+p.S162X+p.K166R+p.D177E+p.I180IV+p.Y181V+p.M184V+p.G196K+p.Q207QH+p.H208HY+p.R211K+p.T215F+p.D218E+p.K219Q+p.L228H	Missense mutation	9.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, T39TA, M41L, K43KQ, V60I, D67N, T69X, K70KR, K101Q, K122E, I135V, S162X, K166R, D177E, I180IV, Y181V, M184V, G196K, Q207QH, H208HY, R211K, T215F, D218E, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.E6EK+p.M41L+p.K43Q+p.K103N+p.K122E+p.I178M+p.V179I+p.M184V+p.T200K+p.Q207E+p.L210W+p.R211K+p.T215Y	Missense mutation	5.4	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, E6EK, M41L, K43Q, K103N, K122E, I178M, V179I, M184V, T200K, Q207E, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3SC+p.V35IT+p.T39TA+p.M41L+p.E44ED+p.S48T+p.D67X+p.S68SG+p.K122KE+p.D123E+p.D177E+p.M184V+p.L210W+p.R211KQ+p.T215Y+p.A272P+p.K277R+p.I293V	Missense mutation	5.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3SC, V35IT, T39TA, M41L, E44ED, S48T, D67X, S68SG, K122KE, D123E, D177E, M184V, L210W, R211KQ, T215Y, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.V35I+p.T39A+p.M41L+p.D67N+p.L74V+p.R83K+p.K101H+p.V118I+p.K122E+p.Y181C+p.M184V+p.G190A+p.T200A+p.E203K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223Q+p.R284K+p.T286N+p.A288S+p.E297K	Missense mutation	5.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, V35I, T39A, M41L, D67N, L74V, R83K, K101H, V118I, K122E, Y181C, M184V, G190A, T200A, E203K, H208Y, L210W, R211K, T215Y, K219N, K223Q, R284K, T286N, A288S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.L74V+p.V118I+p.K122E+p.I135T+p.I142V+p.M184V+p.R211K+p.T215Y+p.D218E+p.K219Q+p.R284K	Missense mutation	5.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, L74V, V118I, K122E, I135T, I142V, M184V, R211K, T215Y, D218E, K219Q, R284K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.D67N+p.T69D+p.K70R+p.K103N+p.K122E+p.D123N+p.M184V+p.T200I+p.T215F+p.K219Q+p.L228H	Missense mutation	6.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, D67N, T69D, K70R, K103N, K122E, D123N, M184V, T200I, T215F, K219Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.K43NS+p.V60I+p.D67N+p.K70KE+p.V75M+p.K102N+p.K103N+p.V106I+p.V108I+p.V118I+p.D123E+p.I135T+p.I142V+p.S162Y+p.M184V+p.V189I+p.G190A+p.G196E+p.E203EA+p.H208HY+p.L210W+p.R211K+p.T215Y+p.K219X+p.H221Y	Missense mutation	5.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, K43NS, V60I, D67N, K70KE, V75M, K102N, K103N, V106I, V108I, V118I, D123E, I135T, I142V, S162Y, M184V, V189I, G190A, G196E, E203EA, H208HY, L210W, R211K, T215Y, K219X, H221Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.V106I+p.I135T+p.K173N+p.Q174K+p.V179E+p.M184V+p.Y188L+p.T200A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.H221Y+p.D237E+p.V245Q	Missense mutation	9.7	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, V106I, I135T, K173N, Q174K, V179E, M184V, Y188L, T200A, H208Y, L210W, R211K, T215Y, H221Y, D237E, V245Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TS+p.M41L+p.K43N+p.V118VI+p.K122E+p.I135LV+p.Y144Y*+p.S162C+p.Y181YC+p.M184V+p.G196GE+p.T200A+p.E203K+p.Q207E+p.L210W+p.R211KNT+p.T215Y+p.K223Q+p.L228H	Missense mutation	5	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TS, M41L, K43N, V118VI, K122E, I135LV, Y144Y*, S162C, Y181YC, M184V, G196GE, T200A, E203K, Q207E, L210W, R211KNT, T215Y, K223Q, L228H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TS+p.V60I+p.S68G+p.T69N+p.A98S+p.D113DN+p.F116Y+p.D123E+p.S134SC+p.I135T+p.N137NI+p.G141GR+p.Q151M+p.M184V+p.I202V+p.F214L+p.V245M	Missense mutation	7.3	Susceptible	[2]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TS, V60I, S68G, T69N, A98S, D113DN, F116Y, D123E, S134SC, I135T, N137NI, G141GR, Q151M, M184V, I202V, F214L, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39X+p.M41L+p.D67N+p.L74V+p.K101H+p.V118I+p.I142V+p.K166Q+p.D177E+p.Y181C+p.M184V+p.V189I+p.G190A+p.Q197E+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223Q+p.V245K+p.A272P+p.P294T+p.E297R+p.L301M	Missense mutation	6.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39X, M41L, D67N, L74V, K101H, V118I, I142V, K166Q, D177E, Y181C, M184V, V189I, G190A, Q197E, H208Y, L210W, R211K, T215Y, K219N, K223Q, V245K, A272P, P294T, E297R, L301M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.A62V+p.K70N+p.A98S+p.K122E+p.D123DN+p.E138Q+p.S162C+p.E169D+p.I178L+p.T200A+p.I202IV+p.R211K+p.T215Y+p.L228R+p.I257L+p.A272P+p.K277R+p.T286A+p.A288S+p.I293V+p.T296S+p.E297R	Missense mutation	4.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, A62V, K70N, A98S, K122E, D123DN, E138Q, S162C, E169D, I178L, T200A, I202IV, R211K, T215Y, L228R, I257L, A272P, K277R, T286A, A288S, I293V, T296S, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.E53EK+p.D67N+p.K70R+p.V118I+p.K122KE+p.A158S+p.K166R+p.V179I+p.M184V+p.L210W+p.R211K+p.T215F+p.K219E+p.V245Q+p.A272P+p.I293V+p.E297A	Missense mutation	9.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, E53EK, D67N, K70R, V118I, K122KE, A158S, K166R, V179I, M184V, L210W, R211K, T215F, K219E, V245Q, A272P, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.K43E+p.K64R+p.D67N+p.L74I+p.V75T+p.K103N+p.V118I+p.K122E+p.M184V+p.G196E+p.T200A+p.E203K+p.Q207E+p.L210W+p.T215Y+p.D218E+p.K219D+p.F227Y+p.L228H+p.L234I+p.T286A+p.I293V+p.Y318F+p.S322A+p.G335D	Missense mutation	7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, K43E, K64R, D67N, L74I, V75T, K103N, V118I, K122E, M184V, G196E, T200A, E203K, Q207E, L210W, T215Y, D218E, K219D, F227Y, L228H, L234I, T286A, I293V, Y318F, S322A, G335D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39E+p.M41L+p.E44D+p.V60I+p.S68G+p.A98G+p.V118I+p.K122E+p.I135T+p.M184V+p.G196E+p.T200A+p.E203K+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K219E	Missense mutation	5.8	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39E, M41L, E44D, V60I, S68G, A98G, V118I, K122E, I135T, M184V, G196E, T200A, E203K, Q207E, L210W, R211K, T215Y, K219E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.T39TA+p.D67DH+p.K70GR+p.R83K+p.A98G+p.A158AS+p.M184MV+p.Q197QH+p.T215X+p.K219KE	Missense mutation	4.9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, T39TA, D67DH, K70GR, R83K, A98G, A158AS, M184MV, Q197QH, T215X, K219KE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IT+p.M41L+p.K43Q+p.D67G+p.S68G+p.T69A+p.K70R+p.L74I+p.K103KN+p.S117SA+p.V118I+p.D121Y+p.K122E+p.I142V+p.R172RK+p.D177G+p.V179I+p.M184V+p.T200A+p.E203EA+p.H208Y+p.L210F+p.R211K+p.T215F+p.D218E+p.K219E+p.L228H+p.A272P+p.K277R+p.Q334L	Missense mutation	4.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IT, M41L, K43Q, D67G, S68G, T69A, K70R, L74I, K103KN, S117SA, V118I, D121Y, K122E, I142V, R172RK, D177G, V179I, M184V, T200A, E203EA, H208Y, L210F, R211K, T215F, D218E, K219E, L228H, A272P, K277R, Q334L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.M41L+p.K43AT+p.S68G+p.L74LI+p.K103S+p.V118I+p.K122E+p.D177G+p.V179I+p.M184V+p.G190A+p.L210W+p.T215Y+p.K219KN+p.K223KQ+p.V245E+p.A272P+p.R284RK	Missense mutation	5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, M41L, K43AT, S68G, L74LI, K103S, V118I, K122E, D177G, V179I, M184V, G190A, L210W, T215Y, K219KN, K223KQ, V245E, A272P, R284RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.T39A+p.M41L+p.S68G+p.L74V+p.K101E+p.K122E+p.I135T+p.I142V+p.D177N+p.Y181C+p.M184V+p.G190A+p.Q207H+p.L210W+p.R211K+p.T215Y+p.H221CY	Missense mutation	5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, T39A, M41L, S68G, L74V, K101E, K122E, I135T, I142V, D177N, Y181C, M184V, G190A, Q207H, L210W, R211K, T215Y, H221CY.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.K49R+p.D67N+p.K70N+p.K122E+p.I135T+p.M184V+p.T200A+p.I202IV+p.T215Y+p.D237DE+p.A272P+p.T286A+p.E297K	Missense mutation	8.5	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, K49R, D67N, K70N, K122E, I135T, M184V, T200A, I202IV, T215Y, D237DE, A272P, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39A+p.M41L+p.R83K+p.V90VI+p.K103KN+p.K122E+p.M184V+p.E203EK+p.Q207E+p.T215Y	Missense mutation	5.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39A, M41L, R83K, V90VI, K103KN, K122E, M184V, E203EK, Q207E, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.D67N+p.A98S+p.K103N+p.V108I+p.K122E+p.I135T+p.A158S+p.M184V+p.I202V+p.L210W+p.T215Y+p.K219R	Missense mutation	7.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, D67N, A98S, K103N, V108I, K122E, I135T, A158S, M184V, I202V, L210W, T215Y, K219R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35X+p.T39TK+p.A62AV+p.K65KR+p.K66X+p.K70KT+p.A98AGS+p.K101KQ+p.I135T+p.Y181C+p.M184MV+p.E203EK+p.Q207E+p.F214L+p.K219H+p.V245EK+p.A272P+p.K275R+p.K277R+p.Q278H+p.I293V	Missense mutation	5.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35X, T39TK, A62AV, K65KR, K66X, K70KT, A98AGS, K101KQ, I135T, Y181C, M184MV, E203EK, Q207E, F214L, K219H, V245EK, A272P, K275R, K277R, Q278H, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Y188L+p.T215Y	Missense mutation	5.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Y188L, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.T69S_SS+p.K70R+p.Q85QH+p.A98S+p.D123E+p.D177E+p.M184V+p.G196R+p.Q207E+p.R211K+p.V245K	Missense mutation	1.5	Susceptible	[22]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, T69S_SS, K70R, Q85QH, A98S, D123E, D177E, M184V, G196R, Q207E, R211K, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.V75T+p.K103N+p.V108VI+p.D123E+p.I135M+p.K166R+p.D177E+p.M184V+p.G190GA+p.G196E+p.R211RG+p.H221HY+p.V245K+p.A272P+p.V292I+p.I293IV+p.E297R	Missense mutation	0.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, V75T, K103N, V108VI, D123E, I135M, K166R, D177E, M184V, G190GA, G196E, R211RG, H221HY, V245K, A272P, V292I, I293IV, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.D123DE+p.E138A+p.N147NT+p.I202V+p.R211K+p.A272P+p.K281R+p.T286A+p.E297K	Missense mutation	0.9	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, D123DE, E138A, N147NT, I202V, R211K, A272P, K281R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.I135T+p.T200A+p.R211K+p.V245M+p.E248D+p.K277R+p.I293V+p.E297A+p.K311R+p.D324G+p.I329L+p.A376S+p.K390R	Missense mutation	1.7	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, I135T, T200A, R211K, V245M, E248D, K277R, I293V, E297A, K311R, D324G, I329L, A376S, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K102KR+p.K122E+p.D123N+p.S162X+p.K173Q+p.M184V+p.T200A+p.I202V+p.R211S+p.L228LH+p.K287R+p.I293V+p.I375V+p.K390R+p.E399D+p.T403M+p.D460N+p.L491S+p.K512Q+p.S519N+p.V548I+p.A554N+p.V559I	Missense mutation	0.3	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K102KR, K122E, D123N, S162X, K173Q, M184V, T200A, I202V, R211S, L228LH, K287R, I293V, I375V, K390R, E399D, T403M, D460N, L491S, K512Q, S519N, V548I, A554N, V559I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K102Q+p.G196E+p.V245K+p.I293V	Missense mutation	1.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K102Q, G196E, V245K, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K103N+p.K122E+p.D123G+p.I132IK+p.S134SR+p.M184V+p.G196E+p.F214FL+p.A288S+p.I293V+p.E297A	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K103N, K122E, D123G, I132IK, S134SR, M184V, G196E, F214FL, A288S, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K103N+p.K122E+p.M184V+p.Q207E+p.T215Y+p.E297K	Missense mutation	1.1	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K103N, K122E, M184V, Q207E, T215Y, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A98S+p.K122E+p.Q207E	Missense mutation	1.1	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A98S, K122E, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D121DY+p.K122E+p.N136ND+p.M184V+p.E203D+p.R211K+p.L283I+p.E297A	Missense mutation	0.7	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D121DY, K122E, N136ND, M184V, E203D, R211K, L283I, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D121H+p.K122E+p.D123E+p.I135V+p.S162C+p.D177E+p.V179D+p.A272P+p.A288S+p.I293V+p.P294PS+p.Q334Y+p.K366R	Missense mutation	0.8	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D121H, K122E, D123E, I135V, S162C, D177E, V179D, A272P, A288S, I293V, P294PS, Q334Y, K366R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D121H+p.K122E+p.S162C+p.D177E+p.M184V+p.R211K	Missense mutation	0.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D121H, K122E, S162C, D177E, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123DE+p.I135T+p.E138A+p.G196GE+p.I202V+p.V245T+p.T286A+p.P294T+p.E297K+p.P313T+p.V317A+p.G333GE+p.Q334Y+p.R356RK+p.K366KR+p.E370D+p.T377M+p.K385R+p.K390R	Missense mutation	2.8	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123DE, I135T, E138A, G196GE, I202V, V245T, T286A, P294T, E297K, P313T, V317A, G333GE, Q334Y, R356RK, K366KR, E370D, T377M, K385R, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I135IT+p.S162C+p.M184V+p.T200TA+p.R211K+p.T215Y	Missense mutation	1.3	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I135IT, S162C, M184V, T200TA, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I135T+p.S162C+p.Y188L+p.G196E+p.T200A+p.T215C+p.A272S+p.V292VI+p.I293V+p.P313AT+p.V317A+p.Q334N	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I135T, S162C, Y188L, G196E, T200A, T215C, A272S, V292VI, I293V, P313AT, V317A, Q334N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I135V+p.M184V+p.R211RK	Missense mutation	0.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I135V, M184V, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I142IF+p.S162C+p.S163I+p.E169E*+p.Y181C+p.M184I+p.T200I+p.R211K+p.E248D+p.A272P+p.K277R+p.T286A+p.P294A+p.E297K+p.E328D+p.Q330QH	Missense mutation	0.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I142IF, S162C, S163I, E169E*, Y181C, M184I, T200I, R211K, E248D, A272P, K277R, T286A, P294A, E297K, E328D, Q330QH.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.I142T+p.D177E+p.V245I+p.D250E+p.I293V+p.A376T+p.A400S	Missense mutation	0.8	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, I142T, D177E, V245I, D250E, I293V, A376T, A400S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.T131TS+p.Q145QL+p.T165L+p.D177E+p.M184V+p.Q207E+p.R211K+p.F214FL	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, T131TS, Q145QL, T165L, D177E, M184V, Q207E, R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.F77L+p.F116FY+p.I135V+p.Q151M+p.M184V+p.G196E+p.Q207K+p.R211A	Missense mutation	8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, F77L, F116FY, I135V, Q151M, M184V, G196E, Q207K, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K219Q	Missense mutation	3.3	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70G+p.K102Q+p.K103KN+p.K122E+p.I135V+p.D177E+p.M184V+p.G196E+p.I202V+p.T215F+p.K219Q+p.E248ED+p.A272G+p.A288S+p.I293V	Missense mutation	0.8	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70G, K102Q, K103KN, K122E, I135V, D177E, M184V, G196E, I202V, T215F, K219Q, E248ED, A272G, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.L74V+p.A98G+p.K103N+p.Y181C+p.M184V+p.K219Q	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, L74V, A98G, K103N, Y181C, M184V, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.L74V+p.A98S+p.L100I+p.K103N+p.I135T+p.K166R+p.M184V+p.R211K+p.F214L+p.K219Q+p.H221Y+p.L228R+p.V245M+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	1.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, L74V, A98S, L100I, K103N, I135T, K166R, M184V, R211K, F214L, K219Q, H221Y, L228R, V245M, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K70R+p.M184V+p.R211K+p.K219E+p.T286A+p.A288G+p.I293IV+p.E297Q	Missense mutation	1.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K70R, M184V, R211K, K219E, T286A, A288G, I293IV, E297Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K101E+p.K122E+p.Y181C+p.G190A+p.I202V+p.T215Y+p.K219N+p.A272P+p.K277R+p.Q278E+p.T286A+p.E297K+p.G335D+p.R356K+p.M357R+p.K366R+p.A376V+p.T386A+p.A400T	Missense mutation	7.3	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K101E, K122E, Y181C, G190A, I202V, T215Y, K219N, A272P, K277R, Q278E, T286A, E297K, G335D, R356K, M357R, K366R, A376V, T386A, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K103S+p.I178M+p.V179I+p.M184V+p.G190A+p.T200TI+p.H208Y+p.R211K+p.T215F+p.K219Q+p.A272P+p.K277R+p.L283LI+p.I293V	Missense mutation	0.9	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K103S, I178M, V179I, M184V, G190A, T200TI, H208Y, R211K, T215F, K219Q, A272P, K277R, L283LI, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.T69D+p.K70R+p.K103N+p.M184V+p.K219Q	Missense mutation	2.8	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, T69D, K70R, K103N, M184V, K219Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E28K+p.K32E+p.D67N+p.R83K+p.K122E+p.S162Y+p.E169D+p.D177E+p.I178L+p.M184V+p.L210W+p.R211K+p.T215Y+p.K219R+p.A272P+p.R284RS	Missense mutation	4.9	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E28K, K32E, D67N, R83K, K122E, S162Y, E169D, D177E, I178L, M184V, L210W, R211K, T215Y, K219R, A272P, R284RS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E40EDV+p.M41L+p.K49R+p.E53EQ+p.K104R+p.K173R+p.T200A+p.Q207X+p.R211K+p.T215E+p.A272P+p.K277R+p.E291D+p.V292VI+p.E297A	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E40EDV, M41L, K49R, E53EQ, K104R, K173R, T200A, Q207X, R211K, T215E, A272P, K277R, E291D, V292VI, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K49KR+p.K122E+p.I135T+p.M184V+p.T200I+p.V245MT+p.I293V+p.E297K	Missense mutation	0.5	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K49KR, K122E, I135T, M184V, T200I, V245MT, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.K122E+p.E169D+p.Q174KR+p.G196E+p.T200I+p.R211K+p.V245L+p.A272P+p.K277KR+p.Q278N+p.I293V+p.K311R+p.D324M	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, K122E, E169D, Q174KR, G196E, T200I, R211K, V245L, A272P, K277KR, Q278N, I293V, K311R, D324M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.M41L+p.T69TA+p.K101Q+p.D123E+p.D177E+p.V179I+p.M184V+p.Q207E+p.R211K+p.T215Y+p.E291D+p.E297K	Missense mutation	5.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, M41L, T69TA, K101Q, D123E, D177E, V179I, M184V, Q207E, R211K, T215Y, E291D, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.T69S+p.L74V+p.K102KR+p.K103N+p.K122E+p.I135L+p.Y181C+p.M184V+p.T200A+p.H221Y+p.V245M+p.A272P+p.R284K+p.T286A+p.I293IV+p.I329L	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, T69S, L74V, K102KR, K103N, K122E, I135L, Y181C, M184V, T200A, H221Y, V245M, A272P, R284K, T286A, I293IV, I329L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V35I+p.R83K+p.I135T+p.Q174R+p.V179VI+p.T200TA+p.Q207D+p.A272S+p.I293V+p.K311R+p.E312V+p.Q334L	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V35I, R83K, I135T, Q174R, V179VI, T200TA, Q207D, A272S, I293V, K311R, E312V, Q334L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V35M+p.D67N+p.T69N+p.K70R+p.A98S+p.T107A+p.D121H+p.K122E+p.I135IT+p.S162A+p.I167V+p.M184V+p.T200I+p.H208Y+p.T215V+p.K219Q+p.A272P+p.T286AG+p.E297K	Missense mutation	4.6	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V35M, D67N, T69N, K70R, A98S, T107A, D121H, K122E, I135IT, S162A, I167V, M184V, T200I, H208Y, T215V, K219Q, A272P, T286AG, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V60I+p.G196E+p.T200I+p.E203D	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V60I, G196E, T200I, E203D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.V60I+p.L74V+p.K103N+p.Y115F+p.K122E+p.Q174K+p.Y181C+p.M184V+p.T200A+p.K219E+p.L228LI+p.V245E+p.N255NH+p.I293V+p.I329L	Missense mutation	0.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, V60I, L74V, K103N, Y115F, K122E, Q174K, Y181C, M184V, T200A, K219E, L228LI, V245E, N255NH, I293V, I329L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6EK+p.K20R+p.V35IM+p.T39A+p.M41L+p.E44D+p.V60I+p.D67N+p.S68SG+p.K70KR+p.V118VI+p.K122KE+p.D123DN+p.S162D+p.I178M+p.V179I+p.M184V+p.G196E+p.L210W+p.R211K+p.T215Y+p.K219E+p.V245M+p.A272P+p.K275R+p.Q278E+p.T286TA+p.P294S+p.E297KR	Missense mutation	3.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6EK, K20R, V35IM, T39A, M41L, E44D, V60I, D67N, S68SG, K70KR, V118VI, K122KE, D123DN, S162D, I178M, V179I, M184V, G196E, L210W, R211K, T215Y, K219E, V245M, A272P, K275R, Q278E, T286TA, P294S, E297KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G15GE+p.K103N+p.I142V+p.R211G+p.F214L+p.P225H+p.A272P+p.T286A+p.K311R+p.V317A+p.Q334Y	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included G15GE, K103N, I142V, R211G, F214L, P225H, A272P, T286A, K311R, V317A, Q334Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135T+p.D177DG+p.R211Q+p.T286A+p.S322T+p.I329V+p.Q334L	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135T, D177DG, R211Q, T286A, S322T, I329V, Q334L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135T+p.G196E+p.V245E+p.A272P+p.K277R+p.I293V+p.P294Q+p.K311R+p.G335E+p.R356K+p.M357R+p.V365I+p.T369A+p.K374R+p.T386I+p.K390R+p.A400T	Missense mutation	1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135T, G196E, V245E, A272P, K277R, I293V, P294Q, K311R, G335E, R356K, M357R, V365I, T369A, K374R, T386I, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I135T+p.I142IV+p.M184V+p.I202V+p.Q207E+p.R211K+p.V241VL	Missense mutation	0.9	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I135T, I142IV, M184V, I202V, Q207E, R211K, V241VL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I142V+p.S162C+p.E169D+p.M184V+p.T200TA+p.Q207X+p.R211K+p.F214FL	Missense mutation	0.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I142V, S162C, E169D, M184V, T200TA, Q207X, R211K, F214FL.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I142V+p.S162C+p.M184V+p.R211K	Missense mutation	0.3	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I142V, S162C, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I178M+p.G196E+p.Q197K+p.V245E+p.T286TA+p.I293V+p.E297EK+p.M357MV+p.S379G+p.K390R	Missense mutation	1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I178M, G196E, Q197K, V245E, T286TA, I293V, E297EK, M357MV, S379G, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I178M+p.M184V	Missense mutation	0.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I178M, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31IR+p.K32R+p.V35I+p.S68N+p.T69A+p.K70T+p.L74V+p.V75T+p.K103N+p.V108I+p.Y115F+p.K122E+p.I135L+p.I142T+p.Q151M+p.Q182NS+p.M184V+p.T200A+p.F214L+p.A272P+p.E297A	Missense mutation	8.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31IR, K32R, V35I, S68N, T69A, K70T, L74V, V75T, K103N, V108I, Y115F, K122E, I135L, I142T, Q151M, Q182NS, M184V, T200A, F214L, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I31L+p.S68SG+p.K103R+p.V179D+p.M184V+p.T200TI+p.R211K+p.F214L+p.T215F+p.V245K+p.I293V	Missense mutation	5.6	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I31L, S68SG, K103R, V179D, M184V, T200TI, R211K, F214L, T215F, V245K, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I50IV+p.V60I+p.K70R+p.R83K+p.K102KR+p.I135T+p.I178L+p.M184V+p.G196E	Missense mutation	0.8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I50IV, V60I, K70R, R83K, K102KR, I135T, I178L, M184V, G196E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101E+p.G190S	Missense mutation	1	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K101E, G190S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K101KQ+p.K122KE+p.S162C+p.F214L+p.A272P+p.K277R+p.K281R+p.T286TA	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K101KQ, K122KE, S162C, F214L, A272P, K277R, K281R, T286TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.D123E+p.M184V+p.T200A+p.Q207E+p.T215Y+p.A272P+p.K277R+p.I293V	Missense mutation	1	Susceptible	[21]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, D123E, M184V, T200A, Q207E, T215Y, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.D123E+p.R211K+p.A272P+p.K277R+p.T286A+p.P294S+p.E297R	Missense mutation	0.7	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, D123E, R211K, A272P, K277R, T286A, P294S, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.D123E+p.Y181C+p.I202V+p.R211K+p.T215Y+p.A272P+p.K277R+p.T286A	Missense mutation	1.4	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, D123E, Y181C, I202V, R211K, T215Y, A272P, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.K122E+p.D177E+p.I178M+p.M184V+p.V245E+p.A272P+p.K277R	Missense mutation	0.6	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, K122E, D177E, I178M, M184V, V245E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.I135IM+p.M184V+p.R211K+p.K277R+p.T286A+p.E297K+p.N348I+p.M357I+p.R358K+p.S379G+p.K390R+p.A400T	Missense mutation	0.4	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, I135IM, M184V, R211K, K277R, T286A, E297K, N348I, M357I, R358K, S379G, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.I142V+p.M184V+p.R211G+p.F214L+p.A272P+p.T286A+p.K311R+p.V317A+p.Q334Y	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, I142V, M184V, R211G, F214L, A272P, T286A, K311R, V317A, Q334Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.K122E+p.M184V+p.F214L+p.A272P+p.K277R	Missense mutation	0.5	Susceptible	[8]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, K122E, M184V, F214L, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103N+p.V108I+p.I135T+p.I178L+p.M184V+p.T200A+p.T215Y+p.A272P	Missense mutation	2.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103N, V108I, I135T, I178L, M184V, T200A, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K103NS+p.K122E+p.I142V+p.E169D+p.M184V+p.T200A+p.Q207E+p.V245K+p.K277RST+p.I293V+p.E297V+p.A304AE+p.D324E+p.Q330P+p.Q334D	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K103NS, K122E, I142V, E169D, M184V, T200A, Q207E, V245K, K277RST, I293V, E297V, A304AE, D324E, Q330P, Q334D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K104KR+p.K122Q+p.D123N+p.I135L+p.S162C+p.V179I+p.R211RK+p.V245I+p.A272S+p.A288S+p.I293V	Missense mutation	0.8	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K104KR, K122Q, D123N, I135L, S162C, V179I, R211RK, V245I, A272S, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K104R+p.D123E+p.I135L+p.S162A+p.K166KN+p.V179I+p.M184V+p.T200E+p.I202V	Missense mutation	0.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K104R, D123E, I135L, S162A, K166KN, V179I, M184V, T200E, I202V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KE+p.D67N+p.K70G+p.V118I+p.D121H+p.K122E+p.I135T+p.V179VI+p.M184V+p.I202V+p.F214L+p.T215F+p.K219Q+p.V245K+p.A272P+p.A288S	Missense mutation	0.8	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KE, D67N, K70G, V118I, D121H, K122E, I135T, V179VI, M184V, I202V, F214L, T215F, K219Q, V245K, A272P, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11KR+p.Q23QR+p.T39A+p.A62P+p.K65R+p.K122E+p.I135T+p.I142V+p.S162A+p.M184V+p.Q207E+p.F214L+p.A272P+p.I274V+p.T286A+p.D324E+p.Q334E	Missense mutation	0.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11KR, Q23QR, T39A, A62P, K65R, K122E, I135T, I142V, S162A, M184V, Q207E, F214L, A272P, I274V, T286A, D324E, Q334E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.K20R+p.V35I+p.V60I+p.S68G+p.T69N+p.D86E+p.W88C+p.K103N+p.D123E+p.K173R+p.I178M+p.T200I+p.D324DE+p.I329V	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, K20R, V35I, V60I, S68G, T69N, D86E, W88C, K103N, D123E, K173R, I178M, T200I, D324DE, I329V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K11R+p.V21I+p.T107S+p.D123E+p.E169D+p.K173D+p.Q174K+p.D177E+p.I178V+p.V179I+p.G190A+p.T200A+p.E203ED+p.D218E+p.A272P+p.V276I+p.L283I+p.V292VI+p.I293V+p.E297A+p.L325I	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K11R, V21I, T107S, D123E, E169D, K173D, Q174K, D177E, I178V, V179I, G190A, T200A, E203ED, D218E, A272P, V276I, L283I, V292VI, I293V, E297A, L325I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.D123N+p.A158S+p.D177E+p.R211RG+p.F214FL+p.T286TN+p.P294T+p.V317A+p.I329V	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, D123N, A158S, D177E, R211RG, F214FL, T286TN, P294T, V317A, I329V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.I135T+p.S162C+p.A272P+p.K277R+p.I293V+p.A304E+p.D324DE	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, I135T, S162C, A272P, K277R, I293V, A304E, D324DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.I142V+p.E169D+p.M184V+p.T200A+p.Q207E+p.V245K+p.I293IV+p.E297V+p.D324E+p.Q334D	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, I142V, E169D, M184V, T200A, Q207E, V245K, I293IV, E297V, D324E, Q334D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.S162C+p.K173Q+p.I293IV	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, S162C, K173Q, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.S162C+p.K173Q	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, S162C, K173Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.S162C+p.M184V+p.I202V+p.R211KQ+p.V245VI+p.D250E+p.A272P+p.K281R+p.T286A+p.S322T+p.Q334E+p.R358RK+p.K390KR+p.E399D+p.A400T	Missense mutation	0.2	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, S162C, M184V, I202V, R211KQ, V245VI, D250E, A272P, K281R, T286A, S322T, Q334E, R358RK, K390KR, E399D, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122KE+p.M184V	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122KE, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K13N+p.V35T+p.T39A+p.K122E+p.M184V+p.T200A+p.L228LR+p.A272S+p.T286A	Missense mutation	0.3	Susceptible	[21]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K13N, V35T, T39A, K122E, M184V, T200A, L228LR, A272S, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K166R+p.K173E+p.I178IV+p.R211K+p.F214L+p.A272P+p.K277R+p.A288S+p.G333E+p.G335S+p.M357T+p.A376T+p.K390R+p.A400T	Missense mutation	0.6	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K166R, K173E, I178IV, R211K, F214L, A272P, K277R, A288S, G333E, G335S, M357T, A376T, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.K43KQ+p.D67N+p.T69ND+p.K70R+p.K122E+p.D123X+p.M184V+p.T200A+p.I202V+p.H208HY+p.T215F+p.K219Q+p.A272P+p.I293IV	Missense mutation	2	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, K43KQ, D67N, T69ND, K70R, K122E, D123X, M184V, T200A, I202V, H208HY, T215F, K219Q, A272P, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.R83K+p.K102KQ+p.K122E+p.R211K+p.V245E+p.A272P+p.K281R+p.T286TA+p.E297K+p.R358K+p.K366R+p.A371AV+p.A376V+p.K395R+p.E396D+p.A400T	Missense mutation	1.1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, R83K, K102KQ, K122E, R211K, V245E, A272P, K281R, T286TA, E297K, R358K, K366R, A371AV, A376V, K395R, E396D, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V21VI+p.M41L+p.K43KN+p.A62AV+p.K70KT+p.V75I+p.W88S+p.D123E+p.D177E+p.I178L+p.M184V+p.G196E+p.V245VE+p.D250E+p.A272P+p.K281R+p.A288S+p.I293IV+p.P294S+p.E297K	Missense mutation	0.3	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V21VI, M41L, K43KN, A62AV, K70KT, V75I, W88S, D123E, D177E, I178L, M184V, G196E, V245VE, D250E, A272P, K281R, A288S, I293IV, P294S, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35VI+p.M41L+p.K43E+p.E44EA+p.K122P+p.D123E+p.I142V+p.E169D+p.M184V+p.G196E+p.T200K+p.H208Y+p.L210W+p.R211K+p.T215Y+p.V245K+p.A272P+p.A288AS+p.E297K	Missense mutation	6.4	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35VI, M41L, K43E, E44EA, K122P, D123E, I142V, E169D, M184V, G196E, T200K, H208Y, L210W, R211K, T215Y, V245K, A272P, A288AS, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V35VRL+p.E36EK+p.S48T+p.V90I+p.A98S+p.D123E+p.E138ED+p.E203ED+p.R211K+p.A272P	Missense mutation	1.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V35VRL, E36EK, S48T, V90I, A98S, D123E, E138ED, E203ED, R211K, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.A33AE+p.V35I+p.T39AS+p.E40ED+p.M41L+p.R83K+p.K103KN+p.D123E+p.I135T+p.S162X+p.M184V+p.I202V+p.T215F+p.A272P+p.K277R+p.S322T	Missense mutation	4.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, A33AE, V35I, T39AS, E40ED, M41L, R83K, K103KN, D123E, I135T, S162X, M184V, I202V, T215F, A272P, K277R, S322T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.A33G+p.D67G+p.T69D+p.K70R+p.V118I+p.K122E+p.I135T+p.M184V+p.Q197P+p.T200A+p.F214L+p.K219Q+p.T286P+p.I293V+p.E297A	Missense mutation	2.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, A33G, D67G, T69D, K70R, V118I, K122E, I135T, M184V, Q197P, T200A, F214L, K219Q, T286P, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E29K+p.M41L+p.K43N+p.E44D+p.D67N+p.L74V+p.L100I+p.K103N+p.V118I+p.K122E+p.D123DE+p.K166R+p.V179VI+p.M184V+p.G196E+p.E203A+p.H208Y+p.L210W+p.R211K+p.T215Y+p.K219N+p.K223T+p.V245E+p.K277R+p.E291D+p.I293V+p.E297A+p.Y318F+p.D324E	Missense mutation	4.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E29K, M41L, K43N, E44D, D67N, L74V, L100I, K103N, V118I, K122E, D123DE, K166R, V179VI, M184V, G196E, E203A, H208Y, L210W, R211K, T215Y, K219N, K223T, V245E, K277R, E291D, I293V, E297A, Y318F, D324E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K101KQ+p.K103N+p.F116Y+p.K122E+p.I135T+p.E138G+p.Q151M+p.Y188H+p.T200E+p.E203D+p.R211K+p.H221Y+p.L228R+p.V245M+p.K277R+p.Q278E+p.T286A+p.E297K+p.Q334L+p.A376T+p.T386A+p.T403A+p.T470N+p.A554T	Missense mutation	7.6	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K101KQ, K103N, F116Y, K122E, I135T, E138G, Q151M, Y188H, T200E, E203D, R211K, H221Y, L228R, V245M, K277R, Q278E, T286A, E297K, Q334L, A376T, T386A, T403A, T470N, A554T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K32E+p.T39A+p.M41L+p.E44D+p.D67N+p.V118I+p.D123E+p.I135K+p.Q174R+p.D177E+p.M184V+p.G196E+p.T200A+p.L210W+p.R211K+p.T215Y+p.V245E+p.E297K	Missense mutation	9.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K32E, T39A, M41L, E44D, D67N, V118I, D123E, I135K, Q174R, D177E, M184V, G196E, T200A, L210W, R211K, T215Y, V245E, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K32KQ+p.M41L+p.E53ED+p.P59PS+p.L74V+p.I135T+p.Q145QR+p.Y181V+p.R211K+p.T215Y+p.K249R	Missense mutation	8.6	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K32KQ, M41L, E53ED, P59PS, L74V, I135T, Q145QR, Y181V, R211K, T215Y, K249R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.K32N+p.V35I+p.M41L+p.K43E+p.E44D+p.D67N+p.V75VA+p.V118I+p.K122E+p.D123DE+p.I135K+p.S162YC+p.K173KE+p.P176PS+p.I178IL+p.M184V+p.G196E+p.Q197KE+p.I202IV+p.L210W+p.R211K+p.T215Y+p.K238KR+p.A272P+p.E291D+p.I293IV+p.P294Q	Missense mutation	4.5	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, K32N, V35I, M41L, K43E, E44D, D67N, V75VA, V118I, K122E, D123DE, I135K, S162YC, K173KE, P176PS, I178IL, M184V, G196E, Q197KE, I202IV, L210W, R211K, T215Y, K238KR, A272P, E291D, I293IV, P294Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.I142V+p.M184V+p.Q207E+p.R211K+p.T215F+p.V245K+p.A272P+p.L301*W	Missense mutation	8.1	Susceptible	[22]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, I142V, M184V, Q207E, R211K, T215F, V245K, A272P, L301*W.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.K122E+p.I135T+p.M184V+p.T200A+p.R211K+p.T215Y+p.A272P+p.I293V+p.E297K	Missense mutation	6.5	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, K122E, I135T, M184V, T200A, R211K, T215Y, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.S48V+p.V60I+p.S68G+p.L74V+p.K101P+p.K103N+p.V118I+p.D121H+p.K122E+p.I135K+p.D177E+p.M184V+p.I202V+p.R211K+p.T215Y+p.V245E+p.K281KE+p.E291D+p.V292I+p.I293V+p.A304G	Missense mutation	2.7	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, S48V, V60I, S68G, L74V, K101P, K103N, V118I, D121H, K122E, I135K, D177E, M184V, I202V, R211K, T215Y, V245E, K281KE, E291D, V292I, I293V, A304G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.V60I+p.K102KR+p.I135AT+p.K166R+p.M184V+p.G196E+p.T200TA+p.T215Y+p.D250E+p.Q278E+p.T296S+p.E297R+p.E298ED+p.E308EV+p.Y319N	Missense mutation	7.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, V60I, K102KR, I135AT, K166R, M184V, G196E, T200TA, T215Y, D250E, Q278E, T296S, E297R, E298ED, E308EV, Y319N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.M41L+p.V60VI+p.D67N+p.K70R+p.I94IL+p.K122E+p.D123G+p.M184V+p.R211K+p.F214L+p.T215F+p.D218E+p.K219Q+p.A272P+p.K277R+p.I293V+p.E297R	Missense mutation	6.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, M41L, V60VI, D67N, K70R, I94IL, K122E, D123G, M184V, R211K, F214L, T215F, D218E, K219Q, A272P, K277R, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.S48T+p.K49R+p.A62V+p.L74V+p.V75T+p.V90I+p.D123E+p.S162DN+p.E169D+p.Y181C+p.R211RK+p.K223KE+p.L228LR+p.V245K+p.A272P+p.K277R+p.L283I+p.I293V+p.P294Q+p.E297K	Missense mutation	0.2	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, S48T, K49R, A62V, L74V, V75T, V90I, D123E, S162DN, E169D, Y181C, R211RK, K223KE, L228LR, V245K, A272P, K277R, L283I, I293V, P294Q, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T39A+p.M41L+p.K43KN+p.L74V+p.K122E+p.M184V+p.L210W+p.R211K+p.T215Y	Missense mutation	4.7	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T39A, M41L, K43KN, L74V, K122E, M184V, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.T69N+p.K70R+p.A98S+p.K103N+p.I142V+p.V245K+p.I293V	Missense mutation	7.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, T69N, K70R, A98S, K103N, I142V, V245K, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.D67G+p.T69D+p.K70R+p.R83K+p.K101P+p.K103N+p.I135K+p.M184V+p.D218E+p.K219Q+p.D250E+p.A272AS+p.T286A+p.P294T	Missense mutation	4.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, D67G, T69D, K70R, R83K, K101P, K103N, I135K, M184V, D218E, K219Q, D250E, A272AS, T286A, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.D67N+p.K70G+p.I142IV+p.S162N+p.K166KQR+p.M184V+p.D218DE+p.K219Q+p.L228LH+p.K277R+p.I293IV+p.K311KR+p.V317VA+p.Q334R+p.G335D+p.P345Q+p.M357T+p.G359S	Missense mutation	0.3	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, D67N, K70G, I142IV, S162N, K166KQR, M184V, D218DE, K219Q, L228LH, K277R, I293IV, K311KR, V317VA, Q334R, G335D, P345Q, M357T, G359S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.E36X+p.T39A+p.D67N+p.T69N+p.K70KR+p.R83K+p.V179I+p.Y181I+p.M184V+p.T215F+p.K219E+p.E248D+p.A272P	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, E36X, T39A, D67N, T69N, K70KR, R83K, V179I, Y181I, M184V, T215F, K219E, E248D, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.E40EQ+p.M41L+p.K43N+p.D67N+p.T69D+p.K103R+p.V118I+p.S162A+p.V179D+p.M184V+p.G196E+p.Q207E+p.F214L+p.T215V+p.K219Q+p.L228H+p.I244V	Missense mutation	0.6	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, E40EQ, M41L, K43N, D67N, T69D, K103R, V118I, S162A, V179D, M184V, G196E, Q207E, F214L, T215V, K219Q, L228H, I244V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35M+p.T39A+p.K49R+p.I63K+p.A98S+p.E138A+p.K166R+p.E169D+p.K173Q+p.V179I+p.Y181I+p.M184V+p.L210W+p.R211K+p.T215Y+p.A272P	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35M, T39A, K49R, I63K, A98S, E138A, K166R, E169D, K173Q, V179I, Y181I, M184V, L210W, R211K, T215Y, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VI+p.K43N+p.A98S+p.K122PQ+p.D123E+p.I135V+p.S162SN+p.T165TI+p.D177E+p.V245KQ+p.A272AP+p.M357V+p.A376T+p.T377M	Missense mutation	1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VI, K43N, A98S, K122PQ, D123E, I135V, S162SN, T165TI, D177E, V245KQ, A272AP, M357V, A376T, T377M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35VI+p.T39TA+p.M41L+p.K43KE+p.L74V+p.K103N+p.V118I+p.K122E+p.D123N+p.I142V+p.M184V+p.G196GE+p.T200A+p.L210W+p.T215Y+p.E248N+p.K277KR+p.K281KR+p.V292I+p.I293V+p.E297A	Missense mutation	9.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35VI, T39TA, M41L, K43KE, L74V, K103N, V118I, K122E, D123N, I142V, M184V, G196GE, T200A, L210W, T215Y, E248N, K277KR, K281KR, V292I, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V60I+p.K64R+p.D67N+p.K70R+p.A98G+p.I159L+p.Q174R+p.Y181C+p.M184V+p.E203K+p.Q207E+p.R211K+p.F214L+p.D218E+p.K219E+p.E224K+p.A272P+p.T286A	Missense mutation	9.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V60I, K64R, D67N, K70R, A98G, I159L, Q174R, Y181C, M184V, E203K, Q207E, R211K, F214L, D218E, K219E, E224K, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K22KN+p.M41L+p.A62V+p.V75T+p.R83K+p.D123E+p.I142V+p.M184V+p.T215Y	Missense mutation	4.6	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K22KN, M41L, A62V, V75T, R83K, D123E, I142V, M184V, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K30KR+p.V35T+p.M41L+p.V60VI+p.D67N+p.T69D+p.V111X+p.K122P+p.I135T+p.N147NT+p.I178M+p.M184V+p.T215Y+p.W252WC+p.A272P+p.I274IT+p.E297A	Missense mutation	6.3	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K30KR, V35T, M41L, V60VI, D67N, T69D, V111X, K122P, I135T, N147NT, I178M, M184V, T215Y, W252WC, A272P, I274IT, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K32E+p.V35I+p.T69A+p.K70KR+p.D121Y+p.K122E+p.I135K+p.I142V+p.A158AS+p.D177E+p.M184V+p.G196GR+p.T200TI+p.Q207E+p.L209LM+p.V245VI+p.A272P+p.T286A	Missense mutation	0.9	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K32E, V35I, T69A, K70KR, D121Y, K122E, I135K, I142V, A158AS, D177E, M184V, G196GR, T200TI, Q207E, L209LM, V245VI, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KE+p.D67N+p.K70R+p.K103R+p.K122E+p.F214FL+p.T215I+p.K219Q+p.T286A+p.A288S+p.E297A	Missense mutation	7.9	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KE, D67N, K70R, K103R, K122E, F214FL, T215I, K219Q, T286A, A288S, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KN+p.K70R+p.I135V+p.M184V+p.R211K	Missense mutation	0.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KN, K70R, I135V, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49KR+p.R83K+p.D123E+p.I135T+p.M184V	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49KR, R83K, D123E, I135T, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.L92F+p.A98S+p.K102Q+p.K103R+p.D121H+p.S162D+p.K166R+p.V179I+p.M184V+p.T200A+p.R211K+p.F214L	Missense mutation	1	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, L92F, A98S, K102Q, K103R, D121H, S162D, K166R, V179I, M184V, T200A, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.V60I+p.R83K+p.I135T+p.K166R+p.M184V+p.A272P+p.K277KR+p.T286A+p.E297EK	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, V60I, R83K, I135T, K166R, M184V, A272P, K277KR, T286A, E297EK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64KR+p.R83RK+p.M184V+p.Q207QK+p.R211DE	Missense mutation	0.5	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64KR, R83RK, M184V, Q207QK, R211DE.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.K122P+p.I135T+p.I159V+p.D177E+p.I202V+p.L210W+p.R211K+p.I293V+p.Q334E+p.R356K+p.M357S+p.S379G+p.K390R	Missense mutation	1.1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, K122P, I135T, I159V, D177E, I202V, L210W, R211K, I293V, Q334E, R356K, M357S, S379G, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K64R+p.T69N+p.K70R+p.R83K+p.D123E+p.I135IV+p.D177E+p.E204K+p.Q207E+p.K277R+p.T286A+p.E297K	Missense mutation	6.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K64R, T69N, K70R, R83K, D123E, I135IV, D177E, E204K, Q207E, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.A98S+p.K122E+p.I135T+p.M184V+p.G196E+p.F214L+p.V245Q+p.I293V+p.E297K	Missense mutation	0.3	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, A98S, K122E, I135T, M184V, G196E, F214L, V245Q, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.D121DY+p.D123DN+p.I135T+p.E138EK+p.S162SC+p.I178IL+p.V179VE+p.M184V+p.Q207E+p.R211K	Missense mutation	0.9	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, D121DY, D123DN, I135T, E138EK, S162SC, I178IL, V179VE, M184V, Q207E, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.I135T+p.M184V+p.R211K+p.F214L	Missense mutation	0.5	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, I135T, M184V, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.I135T+p.Q174L+p.I244V+p.A288S+p.P294T+p.A371V+p.A376T+p.T386TI+p.K390R	Missense mutation	1.4	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, I135T, Q174L, I244V, A288S, P294T, A371V, A376T, T386TI, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.K102R+p.D123E+p.E169D+p.M184V+p.G196E+p.L210F+p.R211RK	Missense mutation	0.8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, K102R, D123E, E169D, M184V, G196E, L210F, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70R+p.K103R+p.M184V+p.R211K+p.F214L	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70R, K103R, M184V, R211K, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M16MV+p.V35I+p.K70N+p.R83RK+p.D121H+p.K122E+p.K166R+p.K173KN+p.D177E+p.I178IM+p.G190A+p.T200TA+p.A272P+p.Q334H+p.G335S	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M16MV, V35I, K70N, R83RK, D121H, K122E, K166R, K173KN, D177E, I178IM, G190A, T200TA, A272P, Q334H, G335S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62AV+p.I135IV+p.M184V+p.L205LV+p.Q207E+p.R211K+p.T215Y	Missense mutation	2	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62AV, I135IV, M184V, L205LV, Q207E, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A62V+p.V75T+p.K122E+p.K173KR+p.M184V+p.L210W+p.T215Y+p.V245E+p.K281R+p.I293V	Missense mutation	1.6	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A62V, V75T, K122E, K173KR, M184V, L210W, T215Y, V245E, K281R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.A98S+p.M184V+p.R211K+p.T215F	Missense mutation	3.9	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, A98S, M184V, R211K, T215F.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.A62AV+p.D123E+p.K166R+p.D177E+p.M184V+p.Q207A+p.R211S+p.T215Y+p.A272P+p.K277R+p.A288S+p.I293V+p.E297K	Missense mutation	3.9	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, A62AV, D123E, K166R, D177E, M184V, Q207A, R211S, T215Y, A272P, K277R, A288S, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.D67N+p.K122E+p.M184V+p.L210W+p.F214L+p.T215Y+p.K219E+p.A272P+p.K277R	Missense mutation	1.7	Susceptible	[8]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, D67N, K122E, M184V, L210W, F214L, T215Y, K219E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.E44D+p.E53D+p.A98S+p.K122A+p.E169D+p.D177E+p.M184V+p.G196E+p.T200A+p.Q207E+p.L210W+p.T215Y	Missense mutation	4.4	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, E44D, E53D, A98S, K122A, E169D, D177E, M184V, G196E, T200A, Q207E, L210W, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.I135V+p.S162C+p.M184V+p.T200A+p.E203K+p.L210W+p.T215Y+p.K275K*+p.R284RS+p.L289LV+p.V292I	Missense mutation	6.9	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, I135V, S162C, M184V, T200A, E203K, L210W, T215Y, K275K*, R284RS, L289LV, V292I.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122E+p.M184V+p.F214L+p.T215Y+p.A272P+p.K277R	Missense mutation	5.5	Susceptible	[8]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122E, M184V, F214L, T215Y, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122KE+p.D123DN+p.A158S+p.E169D+p.M184V+p.T200A+p.T215F+p.A272P+p.L283LI	Missense mutation	2.3	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122KE, D123DN, A158S, E169D, M184V, T200A, T215F, A272P, L283LI.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K122KE+p.K173Q+p.M184V+p.L210LW+p.R211K+p.T215Y+p.K238R+p.V245T+p.A272P+p.K277R+p.T286A+p.E297K	Missense mutation	5.1	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K122KE, K173Q, M184V, L210LW, R211K, T215Y, K238R, V245T, A272P, K277R, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.V75L+p.K122E+p.E138A+p.I142V+p.S162C+p.L168LF+p.R172RG+p.V179VA+p.I180IV+p.M184V+p.T200TA+p.L210W+p.R211Q+p.T215Y+p.L228R	Missense mutation	3.9	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, V75L, K122E, E138A, I142V, S162C, L168LF, R172RG, V179VA, I180IV, M184V, T200TA, L210W, R211Q, T215Y, L228R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.A62AV+p.T69S_ST+p.K101P+p.K103N+p.V118I+p.K122E+p.S162D+p.K166R+p.D177N+p.V179I+p.M184V+p.T200A+p.Q207E+p.L210F+p.R211K+p.T215Y+p.V245E+p.A272P+p.K277R+p.E291D+p.I293V	Missense mutation	9	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, A62AV, T69S_ST, K101P, K103N, V118I, K122E, S162D, K166R, D177N, V179I, M184V, T200A, Q207E, L210F, R211K, T215Y, V245E, A272P, K277R, E291D, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.L74I+p.K103N+p.V179VI+p.M184V+p.T200A+p.L210W+p.R211K+p.T215Y+p.L228Q+p.V245VA+p.D250E+p.T286A+p.I293V+p.E297K	Missense mutation	8.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, L74I, K103N, V179VI, M184V, T200A, L210W, R211K, T215Y, L228Q, V245VA, D250E, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43Q+p.S68G+p.V118I+p.M184V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.L228H+p.K277R+p.T286A+p.I293V+p.E297K	Missense mutation	5.5	Susceptible	[21]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43Q, S68G, V118I, M184V, Q207E, L210W, R211K, T215Y, L228H, K277R, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74LV+p.A98S+p.D121H+p.K122E+p.M184V+p.Q207E+p.L210W+p.R211K+p.T215Y+p.K275KR	Missense mutation	3.2	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74LV, A98S, D121H, K122E, M184V, Q207E, L210W, R211K, T215Y, K275KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74V+p.K102Q+p.D123E+p.Y181C+p.I202V+p.R211K+p.T215Y+p.A272P+p.K277R+p.T286A	Missense mutation	1.6	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74V, K102Q, D123E, Y181C, I202V, R211K, T215Y, A272P, K277R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.L74V+p.K103N+p.D123E+p.Q174K+p.Y181C+p.M184V+p.T200A+p.R211T+p.T215Y+p.V245M+p.A272P+p.I293V+p.E297K	Missense mutation	0.7	Susceptible	[21]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, L74V, K103N, D123E, Q174K, Y181C, M184V, T200A, R211T, T215Y, V245M, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.M184V+p.G196E+p.T200K	Missense mutation	0.8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, M184V, G196E, T200K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.P176S+p.M184V+p.T200A+p.R211K+p.T215Y	Missense mutation	6.2	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, P176S, M184V, T200A, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.Q85R+p.V90I+p.K103N+p.I135V+p.T139R+p.Y181C+p.M184V+p.G196E+p.T200A+p.T215Y	Missense mutation	1.8	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, Q85R, V90I, K103N, I135V, T139R, Y181C, M184V, G196E, T200A, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68G+p.K122P+p.I135L+p.K166R+p.D177E+p.I178L+p.V179I+p.M184V+p.T200A+p.R211K+p.T215Y+p.K277R+p.T286A+p.A288S+p.E297EA	Missense mutation	7.6	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68G, K122P, I135L, K166R, D177E, I178L, V179I, M184V, T200A, R211K, T215Y, K277R, T286A, A288S, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.S68G+p.M184V+p.Q207E+p.T215Y	Missense mutation	2.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, S68G, M184V, Q207E, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V60I+p.S68G+p.D121H+p.K122E+p.I135T+p.K166R+p.D177E+p.M184V+p.G196E+p.L210LW+p.T215Y	Missense mutation	4.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V60I, S68G, D121H, K122E, I135T, K166R, D177E, M184V, G196E, L210LW, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.V90I+p.H96R+p.K103N+p.I135V+p.T139R+p.Q161P+p.Y181C+p.M184V+p.G196E+p.T200A	Missense mutation	0.5	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, V90I, H96R, K103N, I135V, T139R, Q161P, Y181C, M184V, G196E, T200A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.W88C+p.V111I+p.K122E+p.M184V+p.L210W+p.T215Y+p.K275R+p.K277R+p.Q278N+p.L283I+p.I293V	Missense mutation	3.6	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, W88C, V111I, K122E, M184V, L210W, T215Y, K275R, K277R, Q278N, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N137NS+p.M184V+p.V245E+p.T286A+p.E297K	Missense mutation	0.5	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N137NS, M184V, V245E, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P14PS+p.K20R+p.A98S+p.I135IV+p.G196E+p.V245E+p.T286A+p.V292I+p.E297T	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P14PS, K20R, A98S, I135IV, G196E, V245E, T286A, V292I, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1L+p.D67G+p.S68G+p.L74V+p.Y115F+p.D123E+p.I135T+p.I142V+p.D177E+p.M184V+p.T200A+p.T215F+p.K219E+p.V245E+p.E297R	Missense mutation	2.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1L, D67G, S68G, L74V, Y115F, D123E, I135T, I142V, D177E, M184V, T200A, T215F, K219E, V245E, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1PL+p.K11KT+p.K20R+p.V35VI+p.D67N+p.K70G+p.F116Y+p.K122E+p.D123X+p.I135T+p.Q151M+p.I167IV+p.P170PL+p.V179I+p.I202V+p.E204EK+p.H208HY+p.L210LW+p.R211K+p.T215Y+p.T286A+p.E297K	Missense mutation	6.6	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1PL, K11KT, K20R, V35VI, D67N, K70G, F116Y, K122E, D123X, I135T, Q151M, I167IV, P170PL, V179I, I202V, E204EK, H208HY, L210LW, R211K, T215Y, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P1PT+p.V21I+p.P52PL+p.K64KT+p.T107S+p.D123E+p.E169D+p.K173D+p.Q174K+p.D177E+p.I178V+p.V179I+p.G190A+p.T200A+p.E203ED+p.D218E+p.A272P+p.V276I+p.L283I+p.I293V+p.E297A	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P1PT, V21I, P52PL, K64KT, T107S, D123E, E169D, K173D, Q174K, D177E, I178V, V179I, G190A, T200A, E203ED, D218E, A272P, V276I, L283I, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.P4S+p.E6D+p.M41L+p.R172K+p.Y188L+p.V189I+p.Q197K+p.I202V+p.H208Y+p.R211K+p.T215Y+p.V245E+p.A272P+p.E297A	Missense mutation	3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included P4S, E6D, M41L, R172K, Y188L, V189I, Q197K, I202V, H208Y, R211K, T215Y, V245E, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q174H+p.G196E+p.Q207E+p.R211X+p.P294Q+p.K311KR+p.Q334L	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q174H, G196E, Q207E, R211X, P294Q, K311KR, Q334L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q174L+p.V179I+p.Y181C+p.M184V+p.V245M+p.Q278S	Missense mutation	0.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q174L, V179I, Y181C, M184V, V245M, Q278S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q197K+p.Q207E+p.R211K+p.L283I+p.I293V+p.E297K+p.E312A	Missense mutation	1.3	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q197K, Q207E, R211K, L283I, I293V, E297K, E312A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.D123E+p.S162C+p.I178IL+p.M184V+p.T200TA+p.R211T	Missense mutation	0.3	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, D123E, S162C, I178IL, M184V, T200TA, R211T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.D123E+p.V245M+p.D250E+p.A272P+p.I293V+p.V317A+p.M357T+p.A376T+p.E399D+p.A400T	Missense mutation	1.1	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, D123E, V245M, D250E, A272P, I293V, V317A, M357T, A376T, E399D, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.I135IV+p.K166R+p.M184V+p.Q197E+p.I202IV	Missense mutation	0.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, I135IV, K166R, M184V, Q197E, I202IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.I135T+p.R211RG	Missense mutation	1.3	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, I135T, R211RG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K101R+p.I135T+p.E169D+p.M184V+p.R211RS	Missense mutation	0.8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K101R, I135T, E169D, M184V, R211RS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K102R+p.V106I+p.S163T+p.K173N+p.Q174K+p.V179E+p.Y188C+p.Q207E+p.R211K+p.V245T+p.E248ED+p.A272P+p.R284K+p.T286A+p.E291D+p.I293V+p.E297T	Missense mutation	1	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K102R, V106I, S163T, K173N, Q174K, V179E, Y188C, Q207E, R211K, V245T, E248ED, A272P, R284K, T286A, E291D, I293V, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K103N+p.D177E+p.I178LM+p.T200TA+p.Q207E+p.R211K+p.V245K+p.K249Q+p.I293IV+p.V317A	Missense mutation	1.4	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K103N, D177E, I178LM, T200TA, Q207E, R211K, V245K, K249Q, I293IV, V317A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K122E+p.D123DN+p.I135L+p.I178IM+p.M184V+p.T215Y	Missense mutation	2.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K122E, D123DN, I135L, I178IM, M184V, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K122KE+p.D177DG+p.I178IM+p.V245L+p.A272P+p.V276I+p.K277KR+p.A376T+p.A400T	Missense mutation	0.8	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K122KE, D177DG, I178IM, V245L, A272P, V276I, K277KR, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.M184V+p.R211K	Missense mutation	0.5	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.R172RK+p.G196E+p.V245KQ+p.V276VI+p.T286TA+p.I375V+p.A376T+p.T386A+p.A400T	Missense mutation	0.6	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, R172RK, G196E, V245KQ, V276VI, T286TA, I375V, A376T, T386A, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.R211S+p.A272P+p.K281R+p.E297K+p.S322L+p.A376T+p.V381I+p.A400T	Missense mutation	0.7	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, R211S, A272P, K281R, E297K, S322L, A376T, V381I, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.T200TA+p.V245E+p.K281R+p.E297EA	Missense mutation	1.2	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, T200TA, V245E, K281R, E297EA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.I135IT+p.M184V+p.R211K	Missense mutation	0.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, I135IT, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.I135V+p.M184V+p.Q207K+p.R211A	Missense mutation	0.9	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, I135V, M184V, Q207K, R211A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83RK+p.Q85QH+p.K122E+p.S162C+p.I178L+p.I195L+p.T200A+p.E204ED+p.R211K+p.A272P+p.L283I+p.I293V	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83RK, Q85QH, K122E, S162C, I178L, I195L, T200A, E204ED, R211K, A272P, L283I, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S162C+p.I178M+p.M184I+p.G196K+p.R211K+p.A272P+p.Q278H+p.K281R+p.T286A+p.T296S	Missense mutation	0.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S162C, I178M, M184I, G196K, R211K, A272P, Q278H, K281R, T286A, T296S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S3C+p.E6K+p.M41L+p.K43E+p.E44A+p.N54NI+p.D67N+p.K70R+p.V75M+p.K122E+p.S162C+p.E169D+p.M184V+p.G190A+p.T215Y+p.A272P+p.K277R+p.V317A	Missense mutation	8	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S3C, E6K, M41L, K43E, E44A, N54NI, D67N, K70R, V75M, K122E, S162C, E169D, M184V, G190A, T215Y, A272P, K277R, V317A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48SL+p.D123E+p.I135T+p.S162C+p.M184V	Missense mutation	1	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48SL, D123E, I135T, S162C, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48T+p.D67N+p.T69N+p.K70R+p.K122E+p.M184V+p.G196E+p.T215F+p.D218E+p.K219Q+p.V245M+p.A272P+p.A288T+p.E297K+p.E312D	Missense mutation	9.9	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48T, D67N, T69N, K70R, K122E, M184V, G196E, T215F, D218E, K219Q, V245M, A272P, A288T, E297K, E312D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48T+p.K122E+p.I135M+p.M184V+p.P243T	Missense mutation	0.3	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48T, K122E, I135M, M184V, P243T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.F116Y+p.K122E+p.D123E+p.Q151M+p.S162C+p.R172K+p.D177E+p.T200A+p.R211S+p.F214L+p.D250DE+p.K277R+p.A288S	Missense mutation	6.7	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, F116Y, K122E, D123E, Q151M, S162C, R172K, D177E, T200A, R211S, F214L, D250DE, K277R, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.L74V+p.K101Q+p.K103N+p.V108I+p.I135T+p.S162A+p.Y181C+p.M184V+p.R211K+p.K219E+p.P225H+p.L228H+p.V245E+p.A272P+p.V276I+p.K277R+p.K281KR+p.L283I+p.E297A+p.A299AG	Missense mutation	0.2	Susceptible	[22]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, L74V, K101Q, K103N, V108I, I135T, S162A, Y181C, M184V, R211K, K219E, P225H, L228H, V245E, A272P, V276I, K277R, K281KR, L283I, E297A, A299AG.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.T139R+p.M184V+p.V189VI+p.E194EG+p.I195IL+p.R211RK+p.F214L	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, T139R, M184V, V189VI, E194EG, I195IL, R211RK, F214L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68G+p.V90I+p.D123E+p.R211RK	Missense mutation	1.2	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68G, V90I, D123E, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68N+p.I135T+p.M184V+p.T200A+p.Q207G+p.T215D	Missense mutation	0.7	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68N, I135T, M184V, T200A, Q207G, T215D.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S68SG+p.R83K+p.I142IV+p.Y181C+p.M184V+p.I202V+p.Q207E+p.R211K+p.T215Y+p.H221Y+p.T240K+p.V245E+p.A272P+p.K281R+p.P294S	Missense mutation	1.2	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S68SG, R83K, I142IV, Y181C, M184V, I202V, Q207E, R211K, T215Y, H221Y, T240K, V245E, A272P, K281R, P294S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T128A+p.I135T+p.T165I+p.T200A+p.F214L+p.A272T+p.I293V+p.K366R+p.A376T+p.A400T	Missense mutation	0.9	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T128A, I135T, T165I, T200A, F214L, A272T, I293V, K366R, A376T, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.I31V+p.K64H+p.D67N+p.T69N+p.K70R+p.K122E+p.M184V+p.I195L+p.D218E+p.K219Q+p.L228H+p.V245M+p.E248V+p.K249Q+p.A288T+p.I293V	Missense mutation	7.9	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, I31V, K64H, D67N, T69N, K70R, K122E, M184V, I195L, D218E, K219Q, L228H, V245M, E248V, K249Q, A288T, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T27S+p.T39A+p.D67N+p.K70R+p.K102KR+p.D123E+p.I142T+p.D177E+p.M184V+p.G196E+p.Q207QE+p.V245K+p.A272AP	Missense mutation	1.2	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T27S, T39A, D67N, K70R, K102KR, D123E, I142T, D177E, M184V, G196E, Q207QE, V245K, A272AP.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.D67N+p.R83K+p.D123E+p.D177E+p.G196E+p.I202V+p.Q207K+p.R211A+p.T215L+p.K219Q+p.K277R+p.I293V+p.Q334L+p.K390R+p.A400S	Missense mutation	1.2	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, D67N, R83K, D123E, D177E, G196E, I202V, Q207K, R211A, T215L, K219Q, K277R, I293V, Q334L, K390R, A400S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K49R+p.K65R+p.Y115F+p.V118I+p.K122E+p.Y127C+p.I135T+p.E169D+p.Y181C+p.F214L+p.L228H+p.A288S	Missense mutation	0.4	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K49R, K65R, Y115F, V118I, K122E, Y127C, I135T, E169D, Y181C, F214L, L228H, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.K65R+p.R83K+p.K122E+p.K166R+p.D177E+p.I178M+p.M184V+p.Q207K+p.R211K+p.D237E+p.V245E	Missense mutation	0.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, K65R, R83K, K122E, K166R, D177E, I178M, M184V, Q207K, R211K, D237E, V245E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.M41L+p.S68G+p.L74V+p.A98G+p.K103N+p.V118I+p.K122E+p.N137S+p.I142V+p.D177DN+p.M184V+p.T200A+p.L210W+p.T215Y+p.D218E+p.L228N+p.V245E+p.Q278H	Missense mutation	7.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, M41L, S68G, L74V, A98G, K103N, V118I, K122E, N137S, I142V, D177DN, M184V, T200A, L210W, T215Y, D218E, L228N, V245E, Q278H.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.S68G+p.K103N+p.D123E+p.I135T+p.D177E+p.T200A+p.R211K	Missense mutation	1.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, S68G, K103N, D123E, I135T, D177E, T200A, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39A+p.V60I+p.D67N+p.K70R+p.K122E+p.D123N+p.Q174R+p.D177E+p.M184V+p.I195M+p.G196E+p.E203K+p.Q207G+p.F214L+p.K219Q+p.A288S	Missense mutation	2.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39A, V60I, D67N, K70R, K122E, D123N, Q174R, D177E, M184V, I195M, G196E, E203K, Q207G, F214L, K219Q, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39AS+p.I135V+p.S162C+p.M184V+p.T200TA	Missense mutation	0.4	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39AS, I135V, S162C, M184V, T200TA.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T39TA+p.S48E+p.V60VI+p.V90I+p.K103KN+p.S162A+p.M184V+p.Y188L+p.T200A+p.Q207E+p.A272P+p.K277R+p.L283I+p.A288T+p.I293V+p.E297K+p.S322T	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T39TA, S48E, V60VI, V90I, K103KN, S162A, M184V, Y188L, T200A, Q207E, A272P, K277R, L283I, A288T, I293V, E297K, S322T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T7TP+p.D67N+p.K70R+p.K122E+p.D123E+p.I135M+p.Q174E+p.M184V+p.T200A+p.Q207K+p.R211A+p.T215I+p.K219E+p.A272P+p.V292I+p.I293V+p.L301LW+p.E302X	Missense mutation	5.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T7TP, D67N, K70R, K122E, D123E, I135M, Q174E, M184V, T200A, Q207K, R211A, T215I, K219E, A272P, V292I, I293V, L301LW, E302X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V118I+p.D123E+p.I135T+p.R211K+p.F214L+p.A272P+p.K281R+p.T286P+p.I293V+p.E297K	Missense mutation	1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V118I, D123E, I135T, R211K, F214L, A272P, K281R, T286P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.M41L+p.K43KE+p.K122E+p.D177E+p.M184V+p.T200A+p.L210W+p.R211K+p.T215Y	Missense mutation	7.5	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, M41L, K43KE, K122E, D177E, M184V, T200A, L210W, R211K, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.K102KE+p.D123E+p.D177E+p.I178IL+p.V179VI+p.M184V+p.T200TA+p.A272P+p.K277KR+p.T286A+p.E297K+p.A304AS	Missense mutation	0.6	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, K102KE, D123E, D177E, I178IL, V179VI, M184V, T200TA, A272P, K277KR, T286A, E297K, A304AS.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.V35VI+p.R83K+p.I135T+p.S162C+p.D177E+p.I178L+p.I180IV+p.M184V+p.T200A+p.R211RK+p.V245Q+p.A272P+p.K277R+p.K281KR+p.E297A+p.Q334L+p.A376S+p.K390R	Missense mutation	0.7	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, V35VI, R83K, I135T, S162C, D177E, I178L, I180IV, M184V, T200A, R211RK, V245Q, A272P, K277R, K281KR, E297A, Q334L, A376S, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D67N+p.K70R+p.R83K+p.V90I+p.D123E+p.Y181C+p.G196E+p.R211K+p.T215I+p.K219E+p.L228R+p.V245M+p.A272P+p.K275R+p.Q278H+p.V292I+p.I293V+p.E297Q+p.K311R	Missense mutation	5.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D67N, K70R, R83K, V90I, D123E, Y181C, G196E, R211K, T215I, K219E, L228R, V245M, A272P, K275R, Q278H, V292I, I293V, E297Q, K311R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.D67N+p.T69D+p.K70R+p.K103N+p.K122E+p.I135L+p.D177G+p.Y181C+p.T200A+p.H208Y+p.R211K+p.T215F+p.D218E+p.K219Q+p.A272P+p.L283I+p.I293V+p.E297R+p.R356K+p.K366R+p.A371V+p.I375V+p.A376T+p.T377A+p.A400T+p.A446S+p.L491LS+p.I506L	Missense mutation	9.1	Susceptible	[14]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, D67N, T69D, K70R, K103N, K122E, I135L, D177G, Y181C, T200A, H208Y, R211K, T215F, D218E, K219Q, A272P, L283I, I293V, E297R, R356K, K366R, A371V, I375V, A376T, T377A, A400T, A446S, L491LS, I506L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.I50IV+p.R83K+p.K101E+p.K122P+p.K166R+p.D177E+p.Y181C+p.K219N+p.A272P+p.V276I+p.I293V+p.E302D+p.L303W+p.A304Q+p.E305K+p.N306T+p.R307E	Missense mutation	0.4	Susceptible	[22]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, I50IV, R83K, K101E, K122P, K166R, D177E, Y181C, K219N, A272P, V276I, I293V, E302D, L303W, A304Q, E305K, N306T, R307E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K101Q+p.I135IT+p.S162C+p.V179I+p.T200I+p.A272P+p.K277KR	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K101Q, I135IT, S162C, V179I, T200I, A272P, K277KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.K102R+p.D121H+p.K122E+p.I135V+p.S162C+p.D177E+p.I178L+p.Y188H+p.A272S+p.I293V	Missense mutation	2.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, K102R, D121H, K122E, I135V, S162C, D177E, I178L, Y188H, A272S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.K43Q+p.A62V+p.D67N+p.V75I+p.E79D+p.R83K+p.K122E+p.M184V+p.G196E+p.Q197K+p.L210W+p.T215Y+p.D218E+p.K223Q+p.A272P+p.K277R	Missense mutation	7.1	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, K43Q, A62V, D67N, V75I, E79D, R83K, K122E, M184V, G196E, Q197K, L210W, T215Y, D218E, K223Q, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.M41L+p.K43Q+p.A98S+p.K102Q+p.V108I+p.V118I+p.D121Y+p.K122E+p.I135T+p.V179E+p.Y181C+p.M184V+p.T200A+p.L210W+p.T215Y+p.K223Q+p.L228R+p.V245M+p.A272P+p.K275R+p.K277R+p.P294T	Missense mutation	5.2	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, M41L, K43Q, A98S, K102Q, V108I, V118I, D121Y, K122E, I135T, V179E, Y181C, M184V, T200A, L210W, T215Y, K223Q, L228R, V245M, A272P, K275R, K277R, P294T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.Q207E+p.R211K+p.G273GR+p.K275Q+p.I293V+p.D324E+p.I329V	Missense mutation	0.8	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, Q207E, R211K, G273GR, K275Q, I293V, D324E, I329V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.A98S+p.D177E+p.G196E+p.E204X+p.V245M+p.D250E+p.K277R+p.T286A+p.E297K+p.I326V+p.A360T+p.A376S+p.T386I+p.K390R+p.E399G	Missense mutation	0.5	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, A98S, D177E, G196E, E204X, V245M, D250E, K277R, T286A, E297K, I326V, A360T, A376S, T386I, K390R, E399G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.D123E+p.I135V+p.D177E+p.F214L+p.V245E+p.A272P+p.K277R+p.E297K+p.K366R+p.A376T+p.K388KR+p.A400T	Missense mutation	0.9	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, D123E, I135V, D177E, F214L, V245E, A272P, K277R, E297K, K366R, A376T, K388KR, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.R83K+p.K122E+p.I135V+p.S162C+p.A272P+p.Q278N+p.V292I+p.G333E+p.Q334H+p.M357T+p.K366R+p.K390R+p.A400T	Missense mutation	0.6	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, R83K, K122E, I135V, S162C, A272P, Q278N, V292I, G333E, Q334H, M357T, K366R, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S48T+p.K65KR+p.S68SG+p.L74LV+p.V118VI+p.D123E+p.P170PS+p.D177E+p.R211Q+p.V245VM+p.A272P+p.K277R	Missense mutation	0.6	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S48T, K65KR, S68SG, L74LV, V118VI, D123E, P170PS, D177E, R211Q, V245VM, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.S68G+p.T69N+p.K102Q+p.K103S+p.S162C+p.M184V+p.G190A+p.V245Q+p.A272P+p.K277R+p.L283I+p.T286TA+p.I293V+p.E297A	Missense mutation	0.4	Susceptible	[11]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, S68G, T69N, K102Q, K103S, S162C, M184V, G190A, V245Q, A272P, K277R, L283I, T286TA, I293V, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.D67N+p.T69E+p.K70R+p.L74I+p.V75S+p.V118I+p.K122E+p.D123DN+p.I135T+p.S162D+p.V179VI+p.M184V+p.I202IV+p.F214L+p.T215F+p.K219Q+p.K223Q+p.L228H+p.Q278H+p.I293V	Missense mutation	8	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, D67N, T69E, K70R, L74I, V75S, V118I, K122E, D123DN, I135T, S162D, V179VI, M184V, I202IV, F214L, T215F, K219Q, K223Q, L228H, Q278H, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T39A+p.M41L+p.K49R+p.D67N+p.K70KR+p.L74V+p.K101EQ+p.V106VI+p.K122E+p.D123E+p.I135T+p.R172RK+p.V179IT+p.Y181C+p.M184V+p.G190S+p.T215Y+p.D218E+p.K219Q+p.D237E+p.E248D+p.A272AP+p.A288AT+p.I293IV+p.E297A	Missense mutation	2.2	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T39A, M41L, K49R, D67N, K70KR, L74V, K101EQ, V106VI, K122E, D123E, I135T, R172RK, V179IT, Y181C, M184V, G190S, T215Y, D218E, K219Q, D237E, E248D, A272AP, A288AT, I293IV, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.V118I+p.K122E+p.I135T+p.I142V+p.Q151M+p.V179D+p.T200A+p.F214L+p.K277R+p.I293ILV+p.L301LV	Missense mutation	1.9	Susceptible	[19]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, V118I, K122E, I135T, I142V, Q151M, V179D, T200A, F214L, K277R, I293ILV, L301LV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IM+p.T39TA+p.I47IL+p.K49KR+p.I50V+p.R83K+p.K122KE+p.S162SC+p.T165TI+p.T200TA+p.I202V+p.Q207E+p.R211RK+p.A272P+p.K277R+p.V292VI+p.K311KR+p.I329L+p.A360AT+p.K390R+p.A400T	Missense mutation	0.7	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IM, T39TA, I47IL, K49KR, I50V, R83K, K122KE, S162SC, T165TI, T200TA, I202V, Q207E, R211RK, A272P, K277R, V292VI, K311KR, I329L, A360AT, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35IT+p.K103N+p.K122KE+p.S162C+p.D177E+p.I178M+p.R211K+p.T215CS+p.E248N+p.K277R+p.T286A+p.I293V	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35IT, K103N, K122KE, S162C, D177E, I178M, R211K, T215CS, E248N, K277R, T286A, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.K43KR+p.R83K+p.K101KQ+p.K103N+p.D123E+p.G196E+p.P225H+p.A272P+p.K277Q+p.Q278K+p.E297T	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, K43KR, R83K, K101KQ, K103N, D123E, G196E, P225H, A272P, K277Q, Q278K, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35L+p.S48T+p.V60VI+p.T69A+p.A98S+p.K122E+p.I135V+p.T200A+p.Q207E+p.V245E+p.E312Q+p.I329V+p.Q334L	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35L, S48T, V60VI, T69A, A98S, K122E, I135V, T200A, Q207E, V245E, E312Q, I329V, Q334L.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.A62V+p.K122KE+p.T200I+p.L210W+p.R211K+p.T215D+p.E248D+p.A272AT+p.A327T+p.I329L+p.R358K+p.K385R+p.K390R+p.A400T	Missense mutation	0.6	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, A62V, K122KE, T200I, L210W, R211K, T215D, E248D, A272AT, A327T, I329L, R358K, K385R, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.A62V+p.K65R+p.S68X+p.L100I+p.T107S+p.I135T+p.D177DG+p.I178M+p.Y181YC+p.M184V+p.R211RK+p.L228R+p.V245E+p.A272G+p.E297K	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, A62V, K65R, S68X, L100I, T107S, I135T, D177DG, I178M, Y181YC, M184V, R211RK, L228R, V245E, A272G, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K102Q+p.I135V+p.T139R+p.Y181C+p.M184V+p.G196E+p.T200A+p.T215Y+p.L228P	Missense mutation	0.7	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K102Q, I135V, T139R, Y181C, M184V, G196E, T200A, T215Y, L228P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K122KE+p.I135T+p.S162A+p.G196E+p.A272P+p.V276VI+p.K277R+p.I293V+p.E297R	Missense mutation	0.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K122KE, I135T, S162A, G196E, A272P, V276VI, K277R, I293V, E297R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K49R+p.D67N+p.K70R+p.V106I+p.K122E+p.D123N+p.S162D+p.Y181V+p.M184V+p.T200A+p.E203K+p.Q207E+p.K219E+p.L228KQ+p.V245M	Missense mutation	3.5	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K49R, D67N, K70R, V106I, K122E, D123N, S162D, Y181V, M184V, T200A, E203K, Q207E, K219E, L228KQ, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.K65R+p.S68G+p.K82R+p.V106VI+p.K122E+p.I135T+p.G196E+p.R211G+p.V245Q+p.A272P+p.V292I+p.I293IV	Missense mutation	0.6	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, K65R, S68G, K82R, V106VI, K122E, I135T, G196E, R211G, V245Q, A272P, V292I, I293IV.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35M+p.V60I+p.K64R+p.D67N+p.K70R+p.K101R+p.V108I+p.K122P+p.I135T+p.D177E+p.I178L+p.M184V+p.T215F+p.K219Q+p.A272P+p.K277R+p.I293V	Missense mutation	7.7	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35M, V60I, K64R, D67N, K70R, K101R, V108I, K122P, I135T, D177E, I178L, M184V, T215F, K219Q, A272P, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35MT+p.D123N+p.K173Q+p.D177E+p.V245VM+p.A272P+p.K281R+p.T286A+p.I293V+p.S322SA+p.G333E+p.M357T+p.A376T+p.K390R+p.A400S	Missense mutation	0.9	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35MT, D123N, K173Q, D177E, V245VM, A272P, K281R, T286A, I293V, S322SA, G333E, M357T, A376T, K390R, A400S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35R+p.T39A+p.M41ML+p.K102Q+p.K103N+p.D123E+p.I135T+p.I142V+p.S162C+p.M184V+p.T200A+p.P225H+p.V245T+p.A272P+p.I293V+p.E297K	Missense mutation	0.7	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35R, T39A, M41ML, K102Q, K103N, D123E, I135T, I142V, S162C, M184V, T200A, P225H, V245T, A272P, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.D123E+p.I135V+p.T165I+p.K173R+p.I178M+p.V179I+p.K277R+p.A288S+p.E297A+p.I326IM+p.R358K+p.V372I+p.K390R	Missense mutation	0.7	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, D123E, I135V, T165I, K173R, I178M, V179I, K277R, A288S, E297A, I326IM, R358K, V372I, K390R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.K49R+p.K65R+p.A98G+p.K101E+p.K122E+p.A158S+p.E169D+p.V179D+p.G190A+p.R211A+p.V245M	Missense mutation	0.7	Susceptible	[15]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, K49R, K65R, A98G, K101E, K122E, A158S, E169D, V179D, G190A, R211A, V245M.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.K49R+p.V60I+p.D67N+p.T69G+p.A158S+p.M184V+p.G196E+p.L210W+p.T215Y+p.Q278H+p.T286A+p.E297K	Missense mutation	9.9	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, K49R, V60I, D67N, T69G, A158S, M184V, G196E, L210W, T215Y, Q278H, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.M184V+p.T200A+p.R206RK+p.L210W+p.R211RK+p.T215Y+p.K277R+p.T286TA+p.I293V+p.E297K	Missense mutation	5.7	Susceptible	[12]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, M184V, T200A, R206RK, L210W, R211RK, T215Y, K277R, T286TA, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.R83K+p.D177E+p.I178M+p.M184V+p.T215Y+p.V245M+p.E248D+p.D250E+p.A272P+p.T286A	Missense mutation	6.3	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, R83K, D177E, I178M, M184V, T215Y, V245M, E248D, D250E, A272P, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.M41L+p.V60I+p.K103KN+p.V118VI+p.D123E+p.I135IT+p.T139TA+p.T215D+p.V245K	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, M41L, V60I, K103KN, V118VI, D123E, I135IT, T139TA, T215D, V245K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.T39L+p.K43N+p.T69N+p.K102Q+p.K122E+p.I142V+p.S162C+p.I178L+p.T200E+p.Q207E+p.E224D+p.V245L+p.A272P+p.I293V	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, T39L, K43N, T69N, K102Q, K122E, I142V, S162C, I178L, T200E, Q207E, E224D, V245L, A272P, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.V60I+p.K103N+p.K122E+p.I135T+p.G196E+p.Q207E	Missense mutation	1.1	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, V60I, K103N, K122E, I135T, G196E, Q207E.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35TI+p.K65R+p.K103KN+p.K104N+p.Y115YF+p.K122KE+p.D123DE+p.I135IV+p.K166R+p.I202V+p.Q207QH+p.R211HQ+p.T286A	Missense mutation	0.8	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35TI, K65R, K103KN, K104N, Y115YF, K122KE, D123DE, I135IV, K166R, I202V, Q207QH, R211HQ, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.D67N+p.K70R+p.A98S+p.I135IK+p.A158S+p.I159K+p.D177E+p.M184V+p.T200A+p.R211K+p.K219E+p.V245K+p.K249Q+p.T286A+p.I293V+p.E297K+p.E298ED+p.L310LQ+p.S322T	Missense mutation	1.7	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, D67N, K70R, A98S, I135IK, A158S, I159K, D177E, M184V, T200A, R211K, K219E, V245K, K249Q, T286A, I293V, E297K, E298ED, L310LQ, S322T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.R83K+p.V90I+p.K122E+p.S162Y+p.K173E+p.M184I+p.R211RK+p.A272P+p.K277R+p.E297X	Missense mutation	0.3	Susceptible	[4]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, R83K, V90I, K122E, S162Y, K173E, M184I, R211RK, A272P, K277R, E297X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.V60I+p.A98S+p.D123E+p.Q174E+p.I178M+p.T200A+p.E204K+p.V245K+p.D250E+p.A272P+p.K281R+p.I293V+p.Q334E+p.R356RK+p.M357MT+p.K390R+p.A400T	Missense mutation	0.6	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, V60I, A98S, D123E, Q174E, I178M, T200A, E204K, V245K, D250E, A272P, K281R, I293V, Q334E, R356RK, M357MT, K390R, A400T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VI+p.V60VI+p.K64KNY+p.D67N+p.K70R+p.L74V+p.A98AG+p.K103N+p.D121H+p.K122E+p.I142V+p.D177E+p.I178L+p.Y181C+p.M184V+p.Q207E+p.F214L+p.D218E+p.K219Q+p.V245VM+p.A272P+p.E297V	Missense mutation	1.2	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VI, V60VI, K64KNY, D67N, K70R, L74V, A98AG, K103N, D121H, K122E, I142V, D177E, I178L, Y181C, M184V, Q207E, F214L, D218E, K219Q, V245VM, A272P, E297V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35VL+p.M41L+p.K43Q+p.L74V+p.V118I+p.K122P+p.M184V+p.V189I+p.G190Q+p.L210W+p.R211EG+p.T215F+p.L228R+p.A272P+p.P294T+p.E297K	Missense mutation	8	Susceptible	[19]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35VL, M41L, K43Q, L74V, V118I, K122P, M184V, V189I, G190Q, L210W, R211EG, T215F, L228R, A272P, P294T, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D121Y+p.K122E+p.D177E+p.M184V	Missense mutation	0.5	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D121Y, K122E, D177E, M184V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D123X+p.K166T+p.D177DE+p.I178IL+p.T200TA+p.Q207E+p.R211RK	Missense mutation	0.8	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D123X, K166T, D177DE, I178IL, T200TA, Q207E, R211RK.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D67N+p.K70R+p.K103R+p.K104KN+p.I135L+p.S162A+p.M184V+p.T200A+p.Q207E+p.F214L+p.D218DE+p.K219Q+p.A272P+p.K277R+p.A288S	Missense mutation	1.9	Susceptible	[13]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D67N, K70R, K103R, K104KN, I135L, S162A, M184V, T200A, Q207E, F214L, D218DE, K219Q, A272P, K277R, A288S.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.F116Y+p.K122E+p.D123E+p.I135V+p.I142V+p.Q151M+p.T200A+p.I202V+p.F214L+p.V245M+p.K277R+p.Q278E+p.T286A+p.I293V+p.E297T	Missense mutation	9.5	Susceptible	[7]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, F116Y, K122E, D123E, I135V, I142V, Q151M, T200A, I202V, F214L, V245M, K277R, Q278E, T286A, I293V, E297T.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.I142V+p.M184V+p.Q207H+p.R211K	Missense mutation	0.6	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, I142V, M184V, Q207H, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.R83K+p.K166R+p.I178L+p.G196E+p.V245E+p.A272P	Missense mutation	0.9	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, R83K, K166R, I178L, G196E, V245E, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.R83K+p.V90VI+p.A98S+p.K101R+p.K103KN+p.K122KE+p.D123DE+p.E138EA+p.K166R+p.Y181YC+p.G190GA+p.G196EQ+p.R211RK+p.A272P+p.V292I+p.E297K+p.Q334QE+p.P345Q+p.M357X+p.A360T+p.E370ED+p.K390R+p.A400V	Missense mutation	0.4	Susceptible	[20]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, R83K, V90VI, A98S, K101R, K103KN, K122KE, D123DE, E138EA, K166R, Y181YC, G190GA, G196EQ, R211RK, A272P, V292I, E297K, Q334QE, P345Q, M357X, A360T, E370ED, K390R, A400V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.V90I+p.K104N+p.I135IT+p.I142V+p.K166R+p.I202V+p.R211K+p.A272P+p.E297A	Missense mutation	1.3	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, V90I, K104N, I135IT, I142V, K166R, I202V, R211K, A272P, E297A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60VI+p.D67G+p.S68G+p.T69N+p.K70R+p.K101P+p.K102Q+p.K103S+p.I142IV+p.D177E+p.I178L+p.M184V+p.T200A+p.T215V+p.K219E+p.I293V+p.E297K	Missense mutation	5.6	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60VI, D67G, S68G, T69N, K70R, K101P, K102Q, K103S, I142IV, D177E, I178L, M184V, T200A, T215V, K219E, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V8VI+p.V35VI+p.R83K+p.K104KR+p.D123E+p.I135T+p.A272P+p.K281R+p.E297K	Missense mutation	1.2	Susceptible	[9]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V8VI, V35VI, R83K, K104KR, D123E, I135T, A272P, K281R, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V90I+p.K102Q+p.I142V+p.S162SN+p.K166KR+p.E169ED+p.M184V+p.R211K	Missense mutation	0.9	Susceptible	[18]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V90I, K102Q, I142V, S162SN, K166KR, E169ED, M184V, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V90I+p.M184V+p.E203EK+p.Q207QK+p.R211K	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V90I, M184V, E203EK, Q207QK, R211K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A158S+p.Q207QR+p.R211K+p.V245M+p.A272P+p.E297V	Missense mutation	0.9	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A158S, Q207QR, R211K, V245M, A272P, E297V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A62V+p.K65R	Missense mutation	0.5	Susceptible	[33]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A62V, K65R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D123E+p.E138EA+p.I142IM+p.A272P+p.K281R+p.T286A	Missense mutation	0.6	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D123E, E138EA, I142IM, A272P, K281R, T286A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D67N+p.K103N+p.K122KE+p.M184V+p.T215C+p.K219E+p.V245E+p.R284K+p.I293V	Missense mutation	0.5	Susceptible	[25]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D67N, K103N, K122KE, M184V, T215C, K219E, V245E, R284K, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6D+p.K11KN+p.Q207QE+p.R211K+p.K277R+p.K281KR+p.G285GA+p.E291ED	Missense mutation	0.4	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6D, K11KN, Q207QE, R211K, K277R, K281KR, G285GA, E291ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6ED+p.V35R+p.R83K+p.S162C+p.I178L+p.Q207K+p.R211T+p.A272P	Missense mutation	1.1	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6ED, V35R, R83K, S162C, I178L, Q207K, R211T, A272P.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E6G+p.M41L+p.K43N+p.K64H+p.D67G+p.T69N+p.K70R+p.L74I+p.K103N+p.I142V+p.Y181C+p.M184V+p.G196E+p.I202V+p.T215F+p.K219Q+p.V245K+p.K277R+p.A288S+p.I293V	Missense mutation	3.1	Susceptible	[23]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E6G, M41L, K43N, K64H, D67G, T69N, K70R, L74I, K103N, I142V, Y181C, M184V, G196E, I202V, T215F, K219Q, V245K, K277R, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.Q151M+p.S162C+p.K277R	Missense mutation	7.4	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, Q151M, S162C, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.Q151M+p.S162C+p.M184V+p.K277R	Missense mutation	2.1	Susceptible	[16]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, Q151M, S162C, M184V, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.S162C+p.K277R+p.I293V+p.R358K+p.V435I+p.S468P+p.K550G	Missense mutation	0.7	Susceptible	[34]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, S162C, K277R, I293V, R358K, V435I, S468P, K550G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.S162C+p.M184I+p.K277R	Missense mutation	0.3	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, S162C, M184I, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K102Q+p.S162C+p.M184V+p.K277R	Missense mutation	0.4	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K102Q, S162C, M184V, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.8	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.3	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K122E, M184V, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.V35I+p.M41L+p.K43E+p.L74V+p.A98G+p.K101H+p.V108VI+p.V118I+p.D123E+p.I135T+p.D177E+p.Y181C+p.V189VI+p.G190A+p.T200K+p.Q207DE+p.H208Y+p.L210W+p.R211K+p.T215Y+p.E248D+p.A272S+p.K277R+p.I293V	Missense mutation	0.4	Susceptible	[25]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, V35I, M41L, K43E, L74V, A98G, K101H, V108VI, V118I, D123E, I135T, D177E, Y181C, V189VI, G190A, T200K, Q207DE, H208Y, L210W, R211K, T215Y, E248D, A272S, K277R, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K49R+p.R83K+p.Q174H+p.V245E+p.Q269QH+p.A272P+p.K281R+p.K287Q	Missense mutation	1	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K49R, R83K, Q174H, V245E, Q269QH, A272P, K281R, K287Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.K102Q+p.S162C+p.K277R	Missense mutation	0.4	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, K102Q, S162C, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.K122E+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.5	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, K122E, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.3	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, K122E, M184V, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.L74V+p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.3	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, L74V, K122E, M184V, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.S68G	Missense mutation	0.6	Susceptible	[33]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, S68G.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R+p.T69D+p.K70R+p.Y181C+p.G190A	Missense mutation	0.2	Susceptible	[36]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R, T69D, K70R, Y181C, G190A.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K65R	Missense mutation	0.4	Susceptible	[33]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K65R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K70KR+p.K102Q+p.K122E+p.I142T+p.K166R+p.K173KE+p.I178IM+p.A272P+p.K277R	Missense mutation	0.6	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K70KR, K102Q, K122E, I142T, K166R, K173KE, I178IM, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L100I+p.K103N+p.M184V+p.L210W+p.T215Y+p.K219N	Missense mutation	3.1	Susceptible	[36]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L100I, K103N, M184V, L210W, T215Y, K219N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74V+p.K102Q+p.S162C+p.K277R	Missense mutation	1.1	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74V, K102Q, S162C, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74V+p.K122E+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.4	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74V, K122E, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L74V+p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.3	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L74V, K122E, M184V, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.K102Q+p.S162C+p.M184V+p.T215Y+p.K219Q+p.K277R	Missense mutation	4.8	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, K102Q, S162C, M184V, T215Y, K219Q, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.K70R+p.K102Q+p.S162C+p.Y181C+p.T215Y+p.K219Q+p.K277R	Missense mutation	7.3	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, K70R, K102Q, S162C, Y181C, T215Y, K219Q, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.D67N+p.T69D+p.L74V+p.L100I+p.K103N+p.M184V+p.T215Y	Missense mutation	1.3	Susceptible	[36]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, D67N, T69D, L74V, L100I, K103N, M184V, T215Y.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K102Q+p.S162C+p.M184V+p.T215Y+p.K277R	Missense mutation	1.7	Susceptible	[16]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K102Q, S162C, M184V, T215Y, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M41L+p.K43N+p.D67G+p.T69N+p.K70R+p.L74I+p.K103N+p.I142V+p.Y181C+p.M184V+p.G196E+p.I202V+p.T215F+p.K219Q+p.V245K+p.K277R+p.A288S+p.I293V	Missense mutation	3.3	Susceptible	[23]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M41L, K43N, D67G, T69N, K70R, L74I, K103N, I142V, Y181C, M184V, G196E, I202V, T215F, K219Q, V245K, K277R, A288S, I293V.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.K122E+p.K166R+p.I195IR+p.G196E+p.R199RT+p.Q269QH+p.T286A+p.E297K	Missense mutation	0.7	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, K122E, K166R, I195IR, G196E, R199RT, Q269QH, T286A, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R83K+p.Q174H+p.Q207QK+p.R211G+p.V245K+p.A272P+p.K277R+p.R284RS+p.T286A+p.K287Q	Missense mutation	1	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R83K, Q174H, Q207QK, R211G, V245K, A272P, K277R, R284RS, T286A, K287Q.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.S48SL+p.D123DE+p.I135T+p.S162A+p.R211RK+p.V245E+p.A272P+p.V276VI+p.K277R+p.L283LI+p.T286TA+p.E297EA+p.K311KR+p.P313Q+p.V317VA+p.Q334N	Missense mutation	0.6	Susceptible	[25]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included S48SL, D123DE, I135T, S162A, R211RK, V245E, A272P, V276VI, K277R, L283LI, T286TA, E297EA, K311KR, P313Q, V317VA, Q334N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T69N+p.K70R+p.R83K+p.D123E+p.D177E+p.T200TI+p.Q207DE+p.R211K+p.A272P+p.K281R+p.T286A+p.I293V+p.E297K	Missense mutation	6.7	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T69N, K70R, R83K, D123E, D177E, T200TI, Q207DE, R211K, A272P, K281R, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21I+p.S162C+p.Q207E+p.A272P+p.K277R	Missense mutation	0.5	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21I, S162C, Q207E, A272P, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V21VI+p.M41L+p.V75M+p.F77L+p.E79ED+p.K102KE+p.K104R+p.I135V+p.S162DY+p.M184V+p.G196E+p.T200TIM+p.E203D+p.T215FY+p.L283LF+p.R284K+p.T286A+p.I293V+p.E297K	Missense mutation	3.4	Susceptible	[25]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V21VI, M41L, V75M, F77L, E79ED, K102KE, K104R, I135V, S162DY, M184V, G196E, T200TIM, E203D, T215FY, L283LF, R284K, T286A, I293V, E297K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35I+p.T69S+p.K70R+p.R83K+p.I135T+p.K173N+p.G196GE+p.T200TA+p.A272P+p.K277R+p.K281KR	Missense mutation	1.7	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35I, T69S, K70R, R83K, I135T, K173N, G196GE, T200TA, A272P, K277R, K281KR.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.D67N+p.K70R+p.K103N+p.V106A+p.D123E+p.D177E+p.M184V+p.G196E+p.T200A+p.R211N+p.T215F+p.K219Q+p.K277R+p.K281X	Missense mutation	1.9	Susceptible	[23]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, D67N, K70R, K103N, V106A, D123E, D177E, M184V, G196E, T200A, R211N, T215F, K219Q, K277R, K281X.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V35T+p.K43R+p.K64Y+p.D67N+p.K70R+p.L74I+p.K103S+p.V106A+p.D123E+p.D177E+p.M184V+p.G196E+p.T200A+p.R211S+p.T215F+p.K219Q+p.L228H+p.Q242H+p.K277R+p.R284K	Missense mutation	6.7	Susceptible	[23]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V35T, K43R, K64Y, D67N, K70R, L74I, K103S, V106A, D123E, D177E, M184V, G196E, T200A, R211S, T215F, K219Q, L228H, Q242H, K277R, R284K.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V60I+p.D67N+p.K70R+p.I135V+p.K219Q+p.Q269QH+p.E291ED	Missense mutation	8	Susceptible	[24]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V60I, D67N, K70R, I135V, K219Q, Q269QH, E291ED.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V75T+p.K102Q+p.S162C+p.K277R	Missense mutation	0.6	Susceptible	[17]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V75T, K102Q, S162C, K277R.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Y115F+p.K122E+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.9	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Y115F, K122E, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

Key Molecule: HIV1 Reverse transcriptase (HIV1 RT)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Y115F+p.K122E+p.M184V+p.F214L+p.A272P+p.K277R+p.M357T+p.A376T+p.A400T+p.N447S+p.D460N+p.S468T+p.S519N	Missense mutation	0.7	Susceptible	[35]

Sensitive Drug

Zidovudine

Drug Info

Experimental Note

Discovered Using In-vivo Testing Model

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Y115F, K122E, M184V, F214L, A272P, K277R, M357T, A376T, A400T, N447S, D460N, S468T, S519N.

References

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Ref 7	Colinearity of reverse transcriptase inhibitor resistance mutations detected by population-based sequencingJ Acquir Immune Defic Syndr. 2003 Dec 1;34(4):398-402. doi: 10.1097/00126334-200312010-00006.
Ref 8	Natural variation of drug susceptibility in wild-type human immunodeficiency virus type 1Antimicrob Agents Chemother. 2004 Feb;48(2):437-43. doi: 10.1128/AAC.48.2.437-443.2004.
Ref 9	Identification of genotypic changes in human immunodeficiency virus protease that correlate with reduced susceptibility to the protease inhibitor lopinavir among viral isolates from protease inhibitor-experienced patientsJ Virol. 2001 Aug;75(16):7462-9. doi: 10.1128/JVI.75.16.7462-7469.2001.
Ref 10	Human immunodeficiency virus type 1 hypersusceptibility to amprenavir in vitro can be associated with virus load response to treatment in vivoClin Infect Dis. 2001 Dec 15;33(12):2075-7. doi: 10.1086/324510. Epub 2001 Nov 7.
Ref 11	HIV-1 Protease and reverse-transcriptase mutations: correlations with antiretroviral therapy in subtype B isolates and implications for drug-resistance surveillanceJ Infect Dis. 2005 Aug 1;192(3):456-65. doi: 10.1086/431601. Epub 2005 Jul 5.
Ref 12	Comparison of genotyping and phenotyping methods for determining susceptibility of HIV-1 to antiretroviral drugsAIDS. 2001 Aug 17;15(12):1471-5. doi: 10.1097/00002030-200108170-00003.
Ref 13	Efavirenz- and adefovir dipivoxil-based salvage therapy in highly treatment-experienced patients: clinical and genotypic predictors of virologic responseJ Acquir Immune Defic Syndr. 2000 Mar 1;23(3):221-6. doi: 10.1097/00126334-200003010-00002.
Ref 14	Human immunodeficiency virus type 1 reverse-transcriptase and protease subtypes: classification, amino acid mutation patterns, and prevalence in a northern California clinic-based populationJ Infect Dis. 2001 Oct 15;184(8):998-1006. doi: 10.1086/323601. Epub 2001 Sep 10.
Ref 15	Panel of prototypical infectious molecular HIV-1 clones containing multiple nucleoside reverse transcriptase inhibitor resistance mutationsAIDS. 2005 Apr 29;19(7):731-3. doi: 10.1097/01.aids.0000166098.54564.0c.
Ref 16	Hypersusceptibility to non-nucleoside reverse transcriptase inhibitors in HIV-1: clinical, phenotypic and genotypic correlatesAIDS. 2002 Oct 18;16(15):F41-7. doi: 10.1097/00002030-200210180-00002.
Ref 17	A novel phenotypic drug susceptibility assay for human immunodeficiency virus type 1Antimicrob Agents Chemother. 2000 Apr;44(4):920-8. doi: 10.1128/AAC.44.4.920-928.2000.
Ref 18	Rate of thymidine analogue resistance mutation accumulation with zidovudine- or stavudine-based regimensJ Acquir Immune Defic Syndr. 2004 May 1;36(1):600-3. doi: 10.1097/00126334-200405010-00008.
Ref 19	Protease and reverse transcriptase mutation patterns in HIV type 1 isolates from heavily treated persons: comparison of isolates from Northern California with isolates from other regionsAIDS Res Hum Retroviruses. 2003 Oct;19(10):909-15. doi: 10.1089/088922203322493085.
Ref 20	Comparative analysis of two commercial phenotypic assays for drug susceptibility testing of human immunodeficiency virus type 1J Clin Microbiol. 2002 Jan;40(1):31-5. doi: 10.1128/JCM.40.1.31-35.2002.
Ref 21	Loss of antiretroviral drug susceptibility at low viral load during early virological failure in treatment-experienced patientsAIDS. 2000 Dec 22;14(18):2877-87. doi: 10.1097/00002030-200012220-00009.
Ref 22	Mutation patterns and structural correlates in human immunodeficiency virus type 1 protease following different protease inhibitor treatmentsJ Virol. 2003 Apr;77(8):4836-47. doi: 10.1128/jvi.77.8.4836-4847.2003.
Ref 23	Phenotypic evidence for NRTI resistance in HIV-1 isolates with the RT mutation K64H.
Ref 24	Genotypic (and phenotypic) predictors of a response to DDI monotherapy in ZDV-experienced patients.
Ref 25	Miscellaneous Clinical Isolates.
Ref 26	G190A substitution is associated with delavirdine(DLV) hypersusceptibility in vitro and clinical response with DLV intensification.
Ref 27	Characterization of HIV-1 drug resistance development through week 48 in antiretroviral naive subjects on rilpivirine/emtricitabine/tenofovir DF or efavirenz/emtricitabine/tenofovir DF in the STaR study (GS-US-264-0110). J Acquir Immune Defic Syndr. 2014 Mar 1;65(3):318-26. doi: 10.1097/QAI.0000000000000017.
Ref 28	The HIV-1 reverse transcriptase M184I mutation enhances the E138K-associated resistance to rilpivirine and decreases viral fitnessJ Acquir Immune Defic Syndr. 2012 Jan 1;59(1):47-54. doi: 10.1097/QAI.0b013e31823aca74.
Ref 29	Genotypic and phenotypic analyses of human immunodeficiency virus type 1 in antiretroviral drug-naive Nigerian patientsAIDS Res Hum Retroviruses. 2006 Jan;22(1):22-6. doi: 10.1089/aid.2006.22.22.
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Ref 31	Role of the K101E substitution in HIV-1 reverse transcriptase in resistance to rilpivirine and other nonnucleoside reverse transcriptase inhibitors. Antimicrob Agents Chemother. 2013 Nov;57(11):5649-57. doi: 10.1128/AAC.01536-13. Epub 2013 Sep 3.
Ref 32	Antiretroviral drug susceptibility among drug-naive adults with recent HIV infection in Rakai, UgandaAIDS. 2009 Apr 27;23(7):845-52. doi: 10.1097/QAD.0b013e328327957a.
Ref 33	Characterization of resistance mutation patterns emerging over 2 years during first-line antiretroviral treatment with tenofovir DF or stavudine and efavirenz.
Ref 34	Distribution of human immunodeficiency virus type 1 protease and reverse transcriptase mutation patterns in 4,183 persons undergoing genotypic resistance testingAntimicrob Agents Chemother. 2004 Aug;48(8):3122-6. doi: 10.1128/AAC.48.8.3122-3126.2004.
Ref 35	Effect of concurrent zidovudine use on the resistance pathway selected by abacavir-containing regimensHIV Med. 2004 Nov;5(6):394-9. doi: 10.1111/j.1468-1293.2004.00243.x.
Ref 36	HIV-1 reverse transcriptase mutations that suppress zidovudine resistance also increase in vitro susceptibility to tenofovir, but not stavudine.

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Prof. Feng ZHU (zhufeng@zju.edu.cn)