Disease Information

General Information of the Disease (ID: DIS00403)

Name	Ischemia/reperfusion injury
ICD	ICD-11: DB98
Resistance Map

Type(s) of Resistant Mechanism of This Disease

EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s)

2 drug(s) in total

Click to Show/Hide the Full List of Drugs

Hydrogen peroxide

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Key Molecule: Long non-protein coding RNA (ACART)				[1]
Resistant Disease	Ischemia/reperfusion injury [ICD-11: DB98.B]
Molecule Alteration	Down-regulation		Expression	Molecule Info
Resistant Drug	Hydrogen peroxide			Drug Info
Experimental Note	Discovered Using In-vivo Testing Model
Cell Pathway Regulation	PPAR-gama/Bcl-2 signaling pathway		Activation	hsa04933
In Vitro Model	NMVCs	N.A.	.	N.A.
In Vivo Model	C57BL/6 mice and neonatal mice model of I/R injury model			Mus musculus
Experiment for Molecule Alteration	Knockdown assay; Overexpression assay
Experiment for Drug Resistance	MTT assay; TUNEL assay; Lactate dehydrogenase assay
Mechanism Description	LncRNA ACART protects cardiomyocytes from apoptosis by activating PPAR- gama /Bcl-2 pathway.

Morphine

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Key Molecule: TINCR ubiquitin domain containing (TINCR)				[2]
Resistant Disease	Ischemia/reperfusion injury [ICD-11: DB98.B]
Molecule Alteration	Up-regulation		Interaction	Molecule Info
Resistant Drug	Morphine			Drug Info
Experimental Note	Revealed Based on the Cell Line Data
In Vitro Model	H9C2 cells	Ovary	Cricetulus griseus (Chinese hamster)	CVCL_A0TS
In Vivo Model	Rat model of I/R injury			Rattus norvegicus
Experiment for Molecule Alteration	RIP experiments assay; RNA pull down assay; qRT-PCR; Western bloting analysis; Knockdown assay; Overexpression assay
Experiment for Drug Resistance	CCK8 assay; TUNEL assay; Flow cytometry assay
Mechanism Description	MpostC-induced upregulation of TINCR protects cardiomyocytes from I/R injury via inhibiting degradation and ubiquitination of FGF1, and subsequently activating PKCepsilon signaling pathway, which provides a novel insight in the mechanism of TINCR and PKCepsilon during MpostC treatment of I/R injury.

References

Ref 1	LncRNA ACART protects cardiomyocytes from apoptosis by activating PPAR-Gamma/Bcl-2 pathwayJ Cell Mol Med. 2020 Jan;24(1):737-746. doi: 10.1111/jcmm.14781. Epub 2019 Nov 20.
Ref 2	Morphine post-conditioning-induced up-regulation of lncRNA TINCR protects cardiomyocytes from ischemia-reperfusion injury via inhibiting degradation and ubiquitination of FGF1QJM. 2020 Dec 1;113(12):859-869. doi: 10.1093/qjmed/hcaa088.