Molecule Information

General Information of the Molecule (ID: Mol02061)

Name	UDP-N-acetylmuramate-L-alanine ligase (MURC) ,Staphylococcus aureus
Synonyms	murC; USA300HOU_1730 Click to Show/Hide
Molecule Type	Protein
Gene Name	MURC
Sequence	MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALRNKGIKILPFDANNIKE DMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYTSVAVTGAHGKTSTTGLLS HVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLSYKPDYAIMTNIDFDHPDYFK DINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPIYYYGFKDSDDIYAQNIQITDKG TAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISYLEKLDVTNIKEALETFGGVKRRFN ETTIANQVIVDDYAHHPREISATIETARKKYPHKEVVAVFQPHTFSRTQAFLNEFAESLS KADRVFLCEIFGSIRENTGALTIQDLIDKIEGASLINEDSINVLEQFDNAVILFMGAGDI QKLQNAYLDKLGMKNAF Click to Show/Hide
Function	Cell wall formation. Click to Show/Hide
Uniprot ID	MURC_STAAT
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Bacilli Order: 1385 Family: Staphylococcaceae Genus: Staphylococcus Species: Staphylococcus aureus

Type(s) of Resistant Mechanism of This Molecule

UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Diclofenac

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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)		Click to Show/Hide
Disease Class: Staphylococcus aureus infection			[1]
Sensitive Disease	Staphylococcus aureus infection [ICD-11: 1B54.0]		Disease Info
Sensitive Drug	Diclofenac		Drug Info
Molecule Alteration	Expression	Down-regulation
Experimental Note	Discovered Using In-vivo Testing Model
Cell Pathway Regulation	mecA/blaZ pathway	Activation	hsa01501
In Vivo Model	Murine skin and soft tissue infection model		Mus musculus
Experiment for Molecule Alteration	Gene expression analysis; Cellular ATP level assay; Ethidium bromide efflux inhibition assay
Experiment for Drug Resistance	CCK-8 assay
Mechanism Description	High-dose diclofenac can inhibit the growth of MRSA, and does not easily induce drug-resistant mutations after continuous passage. Low-doses diclofenac can resensitize bacteria to beta-lactams, which help to circumvent drug resistance and improve the antibacterial efficacy of conventional antibiotics. Diclofenac can reduce the expression of genes and proteins associated with beta-lactam resistance, low dose diclofenac can inhibit MRSA antibiotic resistance via the mecA/blaZ pathway and related biofilms in implants.

References

Ref 1	Diclofenac Resensitizes Methicillin-Resistant Staphylococcus aureus to Beta-Lactams and Prevents Implant Infections .Adv Sci (Weinh). 2021 May 3;8(13):2100681. doi: 10.1002/advs.202100681. eCollection 2021 Jul. 10.1002/advs.202100681