General Information of the Molecule (ID: Mol02061)
Name
UDP-N-acetylmuramate-L-alanine ligase (MURC) ,Staphylococcus aureus
Synonyms
murC; USA300HOU_1730
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Molecule Type
Protein
Gene Name
MURC
Sequence
MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALRNKGIKILPFDANNIKE
DMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYTSVAVTGAHGKTSTTGLLS
HVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLSYKPDYAIMTNIDFDHPDYFK
DINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPIYYYGFKDSDDIYAQNIQITDKG
TAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISYLEKLDVTNIKEALETFGGVKRRFN
ETTIANQVIVDDYAHHPREISATIETARKKYPHKEVVAVFQPHTFSRTQAFLNEFAESLS
KADRVFLCEIFGSIRENTGALTIQDLIDKIEGASLINEDSINVLEQFDNAVILFMGAGDI
QKLQNAYLDKLGMKNAF
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Function
Cell wall formation.
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Uniprot ID
MURC_STAAT
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Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: 1385
Family: Staphylococcaceae
Genus: Staphylococcus
Species: Staphylococcus aureus
Type(s) of Resistant Mechanism of This Molecule
  UAPP: Unusual Activation of Pro-survival Pathway
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
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Diclofenac
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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
       Unusual Activation of Pro-survival Pathway (UAPP) Click to Show/Hide
Disease Class: Staphylococcus aureus infection [1]
Sensitive Disease Staphylococcus aureus infection [ICD-11: 1B54.0]
Sensitive Drug Diclofenac
Molecule Alteration Expression
Down-regulation
Experimental Note Discovered Using In-vivo Testing Model
Cell Pathway Regulation mecA/blaZ pathway Activation hsa01501
In Vivo Model Murine skin and soft tissue infection model Mus musculus
Experiment for
Molecule Alteration
Gene expression analysis; Cellular ATP level assay; Ethidium bromide efflux inhibition assay
Experiment for
Drug Resistance
CCK-8 assay
Mechanism Description High-dose diclofenac can inhibit the growth of MRSA, and does not easily induce drug-resistant mutations after continuous passage. Low-doses diclofenac can resensitize bacteria to beta-lactams, which help to circumvent drug resistance and improve the antibacterial efficacy of conventional antibiotics. Diclofenac can reduce the expression of genes and proteins associated with beta-lactam resistance, low dose diclofenac can inhibit MRSA antibiotic resistance via the mecA/blaZ pathway and related biofilms in implants.
References
Ref 1 Diclofenac Resensitizes Methicillin-Resistant Staphylococcus aureus to Beta-Lactams and Prevents Implant Infections .Adv Sci (Weinh). 2021 May 3;8(13):2100681. doi: 10.1002/advs.202100681. eCollection 2021 Jul. 10.1002/advs.202100681

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