Molecule Information

General Information of the Molecule (ID: Mol01159)

Name	Multidrug transporter MdfA (MDFA) ,Rhodococcus fascians
Molecule Type	Protein
Gene Name	cmr
Sequence	MPFAIYVLGIAVFAQGTSEFMLSGLIPDMAQDLQVSVPTAGLLTSAFAIGMIIGAPLMAI VSMRWQRRRALLTFLITFMVVHVIGALTDSFGVLLVTRIVGALANAGFLAVALGAAMSMV PADMKGRATSVLLGGVTIACVVGVPGGALLGELWGWRASFWEVVLISAPAVAAIMASTPA DSPTDSVPNATRELSSLRQRKLQLILVLGALINGATFCSFTYLAPTLTDVAGFDSRWIPL LLGLFGLGSFIGVSVGGRLADTRPFQLLVAGSAALLVGWIVFAITASHPVVTLVMLFVQG TLSFAVGSTLISRVLYVADGAPTLGGSFATAAFNVGAALGPALGGVAIGIGMGYRAPLWT SAALVALAIVIGAATWTRWREPRPALDTVPP Click to Show/Hide
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Actinobacteria Class: Actinomycetia Order: Corynebacteriales Family: Nocardiaceae Genus: Rhodococcus Species: Rhodococcus fascians

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Chloramphenicol

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)		Click to Show/Hide
Disease Class: Bacterial infection			[1]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Chloramphenicol		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Escherichia coli MC1061		1211845
	Escherichia coli strain DH5a		668369
	Bacillus subtilis strain BR151		1423
	Rhodococcus fascians strain D188-5		2022521
Experiment for Molecule Alteration	Whole genome sequence assay
Mechanism Description	Rhodococcus fascians NCPPB 1675 (located on the conjugative plasmid pRF2) allowed the identification of two possible open reading frames (ORFs), of which ORF1 was consistent with the mutational analysis. Biochemical analysis of cmr revealed that it does not encode an antibiotic-modifying enzyme. The amino acid sequence of ORF1 predicted a hydrophobic protein, with 12 putative membrane-spanning domains, homologous to proteins involved in the efflux of tetracycline across the plasma membrane.

References

Ref 1	The plasmid-encoded chloramphenicol-resistance protein of Rhodococcus fascians is homologous to the transmembrane tetracycline efflux proteins. Mol Microbiol. 1992 Aug;6(16):2377-85. doi: 10.1111/j.1365-2958.1992.tb01412.x.