Molecule Information

General Information of the Molecule (ID: Mol01101)

Name	Tetracycline efflux Na+/H+ antiporter family transporter Tet(35) (TEE35) ,Vibrio harveyi
Synonyms	AL538_15790 Click to Show/Hide
Molecule Type	Protein
Gene Name	tet(35)
Sequence	MNLIDFAHSPVSLLPPIVALTLAILTRRVLVSLGVGIVLGAVLLNDWSIGNTVGYVSSQV SSVFIEDGGINTWNMSIVGFLILLGMTTALLTLSGGTRAFAEWAQTRVKSKRGSKLLAAF LGVFIFVDDYFNSLAVGAISRPVTDRFYVSRAKLAYILDSTAAPMCVIMPASSWGAYIIT IIGGILVSHGITEYSALGAYVRLIPMNFYAVFALLMVFAVAWFGLDIGKMRDHEIAASQG RGFDKDKENDTQEAHELNEELDIRESEKGKVSDLVLPIVTLIVATIASMLYTGGQALAAD GKEFALLGAFENTDVGTSLIYGSLLGLAVALFTVLKQGLPMVEIARTLWIGAKSMFGAIL ILVFAWTIGSVIGDMKTGSYLSTMAQGNINPHWLPVILFLLSGLMAFSTGTSWGTFGIML PIAGDMAGATDVALMLPMLSAVLAGAVFGDHCSPISDTTILSSTGARCNHIDHVSTQLPY ALSVALVSCVGFIALGMTASIAFSFIAASITFVIVCAILSWLSKSKMASCQNA Click to Show/Hide
Uniprot ID	A0A454BA96_VIBHA
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Proteobacteria Class: Gammaproteobacteria Order: Vibrionales Family: Vibrionaceae Genus: Vibrio Species: Vibrio fluvialis

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Tetracycline

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)		Click to Show/Hide
Disease Class: Vibrio harveyi infection			[1]
Resistant Disease	Vibrio harveyi infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Tetracycline		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Escherichia coli TOP 10		83333
	Vibrio harveyi M3.4L		669
Experiment for Molecule Alteration	RT-PCR
Experiment for Drug Resistance	Agar dilution technique assay
Mechanism Description	We describe the cloning and characterization of two tetracycline resistance determinants from V. harveyi strain M3.4L. The second determinant, cloned as a 3,358-bp fragment in pATJ1, contains two open reading frames, designated tet35 and txr. tet35 encodes a 369-amino-acid protein that was predicted to have nine transmembrane regions. Tetracycline accumulation studies indicate that Escherichia coli carrying tet35 and txr can function as an energy-dependent tetracycline efflux pump but is less efficient than TetA.

References

Ref 1	Genetic determinants of tetracycline resistance in Vibrio harveyi. Antimicrob Agents Chemother. 2002 Apr;46(4):1038-45. doi: 10.1128/AAC.46.4.1038-1045.2002.