Molecule Information

General Information of the Molecule (ID: Mol01070)

Name	Ribosomal RNA large subunit methyltransferase N (CFRC) ,Clostridioides difficile
Synonyms	cfrC; Ribosomal RNA large subunit methyltransferase N Click to Show/Hide
Molecule Type	Protein
Gene Name	cfrC
Sequence	MSKYKKMKQLIADMRLPEYRYKQLLDAVFLQGIMRFEDMKLLPKTLREKLVEQFGETVVE IKAIHHEKSMQTDKVLFELSDGNRVETVGLFYKEGWNSFCISSQSGCGFGCKFCATGTLG LRRNLTVDEITDQILYFMQQGCSINSISFMGMGEPFANPQVFEALHDLTAPELFGLSRRR ITISTIGIVPGIQKLTREYPQVNLAYSLHAPTDRLRETLMPITKTYPLGQVLDTLDQHIR QTNRKVFLAYIMLKDVNDSDRHAEQLTKLLFKHKKYLPLYHLDLIPYNQTTVTETMVPSS HTRIKAFCRIIHNAGISVNIRTQFGSDINAACGQLAGAYRDDQKQGERTMSARDVKSFGE EVCEYGFYNQGAMRSTNSS Click to Show/Hide
Uniprot ID	A0A2R4QH65_CAMCO
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Clostridia Order: Eubacteriales Family: Peptostreptococcaceae Genus: Clostridioides Species: Clostridioides difficile

Type(s) of Resistant Mechanism of This Molecule

ADTT: Aberration of the Drug's Therapeutic Target

Drug Resistance Data Categorized by Drug

Approved Drug(s)

2 drug(s) in total

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Chloramphenicol

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Aberration of the Drug's Therapeutic Target (ADTT)		Click to Show/Hide
Disease Class: Gram-negative bacterial infection			[1]
Resistant Disease	Gram-negative bacterial infection [ICD-11: 1B74-1G40]		Disease Info
Resistant Drug	Chloramphenicol		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Escherichia coli HB101		634468
	Clostridioides difficile T10		1215084
	Clostridium bolteae 90B3		997895
	Escherichia coli TG1		562
Experiment for Molecule Alteration	Whole genome sequence assay
Experiment for Drug Resistance	Disk diffusion methods assay; agar dilution methods assay
Mechanism Description	The cfr gene encodes a 23S rRNA methyltransferase, which causes C-8 modification in A2503 located in the peptidyl transferase region of bacterial ribosome.This mechanism confers PhLOPSA (phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A) resistance.

Florfenicol

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Aberration of the Drug's Therapeutic Target (ADTT)		Click to Show/Hide
Disease Class: Gram-negative bacterial infection			[1]
Resistant Disease	Gram-negative bacterial infection [ICD-11: 1B74-1G40]		Disease Info
Resistant Drug	Florfenicol		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Escherichia coli HB101		634468
	Clostridioides difficile T10		1215084
	Clostridium bolteae 90B3		997895
	Escherichia coli TG1		562
Experiment for Molecule Alteration	Whole genome sequence assay
Experiment for Drug Resistance	Disk diffusion methods assay; agar dilution methods assay
Mechanism Description	The cfr gene encodes a 23S rRNA methyltransferase, which causes C-8 modification in A2503 located in the peptidyl transferase region of bacterial ribosome.This mechanism confers PhLOPSA (phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A) resistance.

References

Ref 1	A cfr-like gene cfr(C) conferring linezolid resistance is common in Clostridium difficile. Int J Antimicrob Agents. 2017 Sep;50(3):496-500. doi: 10.1016/j.ijantimicag.2017.03.013. Epub 2017 Jun 27.