Molecule Information

General Information of the Molecule (ID: Mol01069)

Name	Ribosomal RNA large subunit methyltransferase Cfr (CFRB) ,Enterococcus faecium
Synonyms	23S rRNA (adenine(2503)-C(8))-methyltransferase; 23S rRNA m8A2503 methyltransferase; cfr Click to Show/Hide
Molecule Type	Protein
Gene Name	cfr(B)
Sequence	MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITVLPKSLRDMLIEEFGES ILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESFCISSQCGCNFGCKFCATG DIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALANVQVFDALNVLTDPALFALS PRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQRSELMPINERYPLSDVMDTLDE HIRVTSRKVYIAYIMLHGVNDSIEHAKEVVNLLRGRYRSGNLYHVNIIRYNPTVSSRMRF EEANEKCLVNFYKKLKSAGIKVTIRSQFGIDIDAACGQLYGNYQKTNSQ Click to Show/Hide
Function	Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics. Click to Show/Hide
Uniprot ID	A0A0K1TBR6_ENTFC
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Bacilli Order: Lactobacillales Family: Enterococcaceae Genus: Enterococcus Species: Enterococcus faecalis

Type(s) of Resistant Mechanism of This Molecule

ADTT: Aberration of the Drug's Therapeutic Target

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Clindamycin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Aberration of the Drug's Therapeutic Target (ADTT)		Click to Show/Hide
Disease Class: Bacterial infection			[1]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Clindamycin		Drug Info
Molecule Alteration	Missense mutation	c.2576G>T
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Staphylococcus aureus ATCC 29213		1280
	Enterococcus faecium ATCC 29212		1352
	Enterococcus faecium ATCC 35667		1352
Experiment for Molecule Alteration	Whole genome sequence assay
Experiment for Drug Resistance	MIC assay
Mechanism Description	Cfr methylates the unreactive C2- and C8-carbon atoms on the A2503 residue located in a functionally critical region of the 23S rRNA component.The methylation at C8 protects the Cfr-producing bacteria from the action of five major classes of antibiotics, namely, phenicols, oxazolidinones, pleuromutilins, macrolides, and streptogramin A compounds (PhLOPSA phenotype).

References

Ref 1	Detection of a New cfr-Like Gene, cfr(B), in Enterococcus faecium Isolates Recovered from Human Specimens in the United States as Part of the SENTRY Antimicrobial Surveillance Program. Antimicrob Agents Chemother. 2015 Oct;59(10):6256-61. doi: 10.1128/AAC.01473-15. Epub 2015 Jul 27.