Molecule Information

General Information of the Molecule (ID: Mol01003)

Name	Metallo-beta-lactamase NDM-4 (NDM4) ,Escherichia coli
Synonyms	bla(NDM-4); blaNDM-4; NDM-4; NDM-4; New Delhi metallo-beta-lactamase NDM-4 Click to Show/Hide
Molecule Type	Protein
Gene Name	bla(NDM-4)
Sequence	MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQ HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTH AHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTFAANGWVEPATAPNFGPL KVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAF PKASMIVMSHSAPDSRAAITHTARMADKLR Click to Show/Hide
Uniprot ID	H6WZS9_ECOLX
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Kingdom: N.A. Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Escherichia Species: Escherichia coli

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Ticarcillin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Ticarcillin		Drug Info
Molecule Alteration	Missense mutation	p.M154L
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Escherichia coli I5		562
Experiment for Molecule Alteration	Whole genome sequence assay; Allelic frequency measurement assay
Experiment for Drug Resistance	Disk diffusion test assay; E-strip test assay
Mechanism Description	A clinical Escherichia coli isolate resistant to all Beta-lactams, including carbapenems, expressed a novel metallo-Beta-lactamase (MBL), NDM-4, differing from NDM-1 by a single amino acid substitution (Met154Leu). NDM-4 possessed increased hydrolytic activity toward carbapenems and several cephalosporins compared to that of NDM-1.

References

Ref 1	NDM-4 metallo-Beta-lactamase with increased carbapenemase activity from Escherichia coli. Antimicrob Agents Chemother. 2012 Apr;56(4):2184-6. doi: 10.1128/AAC.05961-11. Epub 2012 Jan 17.