Molecule Information

General Information of the Molecule (ID: Mol00870)

• Name: Chloramphenicol acetyltransferase (CAT) ,Bacillus pumilus
• Synonyms: CAT; Cat-86
• Molecule Type: Protein
• Gene Name: cat86
• Sequence:
  MFKQIDENYLRKEHFHHYMTLTRCSYSLVINLDITKLHAILKEKKLKVYPVQIYLLARAV
  QKIPEFRMDQVNDELGYWEILHPSYTILNKETKTFSSIWTPFDENFAQFYKSCVADIETF
  SKSSNLFPKPHMPENMFNISSLPWIDFTSFNLNVSTDEAYLLPIFTIGKFKVEEGKIILP
  VAIQVHHAVCDGYHAGQYVEYLRWLIEHCDEWLNDSLHIT
• Function: This enzyme is an effector of chloramphenicol resistance in bacteria.
• Uniprot ID: CAT_BACPU

Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Species: Bacillus pumilus

Type(s) of Resistant Mechanism of This Molecule

  DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Chloramphenicol

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Disease Class: Bacillus pumilus infection [1]

• Resistant Disease: Bacillus pumilus infection [ICD-11: 1C4Y.3]
• Resistant Drug: Chloramphenicol
• Molecule Alteration: Expression; Inherence
• Experimental Note: Discovered Using In-vivo Testing Model
• In Vitro Model: Bacillus pumilus strain NCIB8600; 1408
• In Vitro Model: Bacillus subtilis strain BR151; 1423
• Experiment for Molecule Alteration: Restriction enzyme assay
• Mechanism Description: Genes (cat) specifying the enzyme CAT occur in a wide range of unrelated bacteria, where they confer resistance to the antibiotic Cm. Gene cat-86 of Bacillus pumilus, specifying chloramphenicol-inducible chloramphenicol acetyltransferase, was previously cloned in Bacillus subtilis on plasmid pUB110.

References

• Ref 1: Nucleotide sequence of a Bacillus pumilus gene specifying chloramphenicol acetyltransferase. Gene. 1983 Oct;24(2-3):163-9. doi: 10.1016/0378-1119(83)90076-8.