Molecule Information

General Information of the Molecule (ID: Mol00867)

• Name: Chloramphenicol acetyltransferase (CAT) ,Staphylococcus intermedius
• Synonyms: CAT
• Molecule Type: Protein
• Gene Name: cat
• Sequence:
  MTFNIIKLENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKKKGYEIYPSLIYAIMEV
  VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTSFYNNYKNDLF
  EYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIY
  IPVALQLHHAVCDGYHASLFINEFQDIIKKVDDWI
• Function: This enzyme is an effector of chloramphenicol resistance in bacteria.
• Uniprot ID: CAT_STAIN

Kingdom: N.A.
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Staphylococcaceae
Genus: Staphylococcus
Species: Staphylococcus intermedius

Type(s) of Resistant Mechanism of This Molecule

  DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s) 1 drug(s) in total

Chloramphenicol

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Drug Inactivation by Structure Modification (DISM)

Disease Class: Staphylococcus intermedius infection [1]

• Resistant Disease: Staphylococcus intermedius infection [ICD-11: 1B54.3]
• Resistant Drug: Chloramphenicol
• Molecule Alteration: Expression; Inherence
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Escherichia coli strain XL-1 Blue; 562
• In Vitro Model: A Staphylococcus intermedius strain isolated from a purulent skin infection of a dog; 1285
• Experiment for Molecule Alteration: Dideoxy chain-termination method assay
• Mechanism Description: However, little is known about CmR in staphylococcal species pathogenic to animals. Recently, CmR plasmids have been isolated from 'equine's. sciuri, 'canine' S. epidermidis, 'porcine' S. hyicus and 'canine' S. intermedius strains. All staphy- lococcal CmR plasmids encode a common resistance mechanism, namely an inducible Cm acetyltransferase (CAT).

Disease Class: Escherichia coli infection [1]

• Resistant Disease: Escherichia coli infection [ICD-11: 1A03.0]
• Resistant Drug: Chloramphenicol
• Molecule Alteration: Expression; Acquired
• Experimental Note: Identified from the Human Clinical Data
• In Vitro Model: Escherichia coli strain XL-1 Blue; 562
• In Vitro Model: A Staphylococcus intermedius strain isolated from a purulent skin infection of a dog; 1285
• Experiment for Molecule Alteration: Dideoxy chain-termination method assay
• Mechanism Description: Subsequently, Escherichia coli XL-1 blue cells transformed with these recombinant plasmids were tested for CmR. In one orientation, Escherichia coli XL-1 blue demonstrated CmR at 15 ug/ml Cm while in the other orientation a higher level of CmR occurred (80 ug/m Cm).

References

• Ref 1: Cloning and sequence analysis of a plasmid-encoded chloramphenicol acetyltransferase gene from Staphylococcus intermedius. J Gen Microbiol. 1991 Apr;137(4):977-81. doi: 10.1099/00221287-137-4-977.