Molecule Information

General Information of the Molecule (ID: Mol00838)

Name	Beta-lactamase (BLA) ,Escherichia coli
Synonyms	Penicillinase; oxa2 Click to Show/Hide
Molecule Type	Protein
Gene Name	bla
Gene ID	67369352
Sequence	MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTIVVADERQADRAMLVFD PVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNRGFAGHNQDQDLRSAMRNS TVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWIEGSLAISAQEQIAFLRKLYR NELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMGWWVGWVEWPTGSVFFALNIDTP NRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR Click to Show/Hide
Function	This is an oxacillin-hydrolyzing beta-lactamase. Click to Show/Hide
Uniprot ID	BLO2_ECOLX
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Escherichia Species: Escherichia coli

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

2 drug(s) in total

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Amoxicillin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Gram-negative bacterial infection			[1], [2]
Resistant Disease	Gram-negative bacterial infection [ICD-11: 1B74-1G40]		Disease Info
Resistant Drug	Amoxicillin		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Escherichia coli		668369
	Acinetobacter johnsonii LIM75		40214
	Aeromonas allosaccharophila LIM82		656
	Citrobacter freundii LIM86		546
	Escherichia coli MFDpir		562
Experiment for Molecule Alteration	Whole genome sequence assay
Experiment for Drug Resistance	Agar dilution method assay; broth microdilution method assay
Mechanism Description	All known class 3 integrons harbour gene cassettes encoding resistance to Beta-lactams (blaIMP, blaGES, blaBEL, blaOXA-256) and aminoglycosides [aac(6')-Ib].

Ceftazidime

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Gram-negative bacterial infection			[1], [2]
Resistant Disease	Gram-negative bacterial infection [ICD-11: 1B74-1G40]		Disease Info
Resistant Drug	Ceftazidime		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Escherichia coli		668369
	Acinetobacter johnsonii LIM75		40214
	Aeromonas allosaccharophila LIM82		656
	Citrobacter freundii LIM86		546
	Escherichia coli MFDpir		562
Experiment for Molecule Alteration	Whole genome sequence assay
Experiment for Drug Resistance	Agar dilution method assay; broth microdilution method assay
Mechanism Description	All known class 3 integrons harbour gene cassettes encoding resistance to Beta-lactams (blaIMP, blaGES, blaBEL, blaOXA-256) and aminoglycosides [aac(6')-Ib].

References

Ref 1	Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg. Int J Antimicrob Agents. 2016 Oct;48(4):431-4. doi: 10.1016/j.ijantimicag.2016.06.018. Epub 2016 Jul 28.
Ref 2	Improved purification and characterization of the OXA-2 beta-lactamase. Biochem J. 1984 Dec 15;224(3):1009-13. doi: 10.1042/bj2241009.