Molecule Information

General Information of the Molecule (ID: Mol00729)

Name	Protein tcr3 (TCR3) ,Streptomyces aureofaciens
Synonyms	tcr3; Tcr3 protein Click to Show/Hide
Molecule Type	Protein
Gene Name	tcr3
Sequence	MGMANATSQTGEAVADEAGGPAGFTHRQIITALSGLLLAVLLAALDQTIVSTALRTIGDQ LHGQTVQAWVITGYLVSSTIAMPFYGKLSDIYGRKPLYLAAIAVFIVGSAACAMANSMET LAIARVLQGFGGAGLMSLPTAVIADLAPVRERGRYFSYLMMAWVAASVLGPLVGGLFAGA GEILGVTGWRWAFLINVPLGLVALLSVRKALNLPHRRVDHPIDFRGALTLALCLVPLLIV AEEGLDWGWGSARSLTLFAVSLIGLVLFVLAERARGLEAMVPLRLFRRGGITMATAVNFT IGVGIFGTVSTLPLFLQLVQGRSATVAGLVIIPVMTGAIVSQTICAKIIKKWNRYKKPAI VGLGSMAGALLSLSAAGADTPLAVIVVIAAWLGFGIGLSQTVITLAIQSSAPKSELGVAN AASGLFRQLGGTSGAAVFMSVLFGVAAGRLDGADPDEAVRRALSDPGSTGGLSASAVDAF TSGFDTMFLVGGLILAVGFLLTFPLRELRDEE Click to Show/Hide
Uniprot ID	Q53769_KITAU
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Actinobacteria Class: Actinomycetia Order: Streptomycetales Family: Streptomycetaceae Genus: Kitasatospora Species: Kitasatospora aureofaciens

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

Click to Show/Hide the Full List of Drugs

Tetracycline

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)		Click to Show/Hide
Disease Class: Streptomyces aurebyaciens infection			[1]
Resistant Disease	Streptomyces aurebyaciens infection [ICD-11: 1C43.2]		Disease Info
Resistant Drug	Tetracycline		Drug Info
Molecule Alteration	Expression	Inherence
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Escherichia coli strain XLI-Blue		562
	Streptomyces aureofaciens strain NRRL3203		1894
	Streptomyces lividans strain Tk23		1916
Experiment for Molecule Alteration	Northern blotting analysis
Mechanism Description	The analysis of the nucleotide sequence of the 2.8-kb BamHI fragment containing tcrC gene showed that the predicted tcrC gene product is a protein consisting of 512 amino acids. The deduced amino acid sequence had a high level identity with that of the self-defense gene (tet347) of Streptomyces rimosus, known to mediate oxytetracycline efflux. The tcrC gene-inactivated strains generated from strain NRRL3203 by gene replacement had a 90% decrease in the level of resistance to tetracycline and the antibiotic productivity when compared with the parental strain.

References

Ref 1	A self-defense gene homologous to tetracycline effluxing gene essential for antibiotic production in Streptomyces aureofaciens. Biosci Biotechnol Biochem. 1995 Oct;59(10):1835-41. doi: 10.1271/bbb.59.1835.