Disease Information

General Information of the Disease (ID: DIS00403)

• Name: Ischemia/reperfusion injury
• ICD: ICD-11: DB98

Type(s) of Resistant Mechanism of This Disease

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s) 2 drug(s) in total

Hydrogen peroxide

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Key Molecule: Long non-protein coding RNA (ACART) [1]

• Resistant Disease: Ischemia/reperfusion injury [ICD-11: DB98.B]
• Molecule Alteration: Down-regulation; Expression
• Resistant Drug: Hydrogen peroxide
• Experimental Note: Discovered Using In-vivo Testing Model
• Cell Pathway Regulation: PPAR-gama/Bcl-2 signaling pathway; Activation; hsa04933
• In Vitro Model: NMVCs; N.A.; .; N.A.
• In Vivo Model: C57BL/6 mice and neonatal mice model of I/R injury model; Mus musculus
• Experiment for Molecule Alteration: Knockdown assay; Overexpression assay
• Experiment for Drug Resistance: MTT assay; TUNEL assay; Lactate dehydrogenase assay
• Mechanism Description: LncRNA ACART protects cardiomyocytes from apoptosis by activating PPAR- gama /Bcl-2 pathway.

Morphine

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Key Molecule: TINCR ubiquitin domain containing (TINCR) [2]

• Resistant Disease: Ischemia/reperfusion injury [ICD-11: DB98.B]
• Molecule Alteration: Up-regulation; Interaction
• Resistant Drug: Morphine
• Experimental Note: Revealed Based on the Cell Line Data
• In Vitro Model: H9C2 cells; Ovary; Cricetulus griseus (Chinese hamster); CVCL_A0TS
• In Vivo Model: Rat model of I/R injury; Rattus norvegicus
• Experiment for Molecule Alteration: RIP experiments assay; RNA pull down assay; qRT-PCR; Western bloting analysis; Knockdown assay; Overexpression assay
• Experiment for Drug Resistance: CCK8 assay; TUNEL assay; Flow cytometry assay
• Mechanism Description: MpostC-induced upregulation of TINCR protects cardiomyocytes from I/R injury via inhibiting degradation and ubiquitination of FGF1, and subsequently activating PKCepsilon signaling pathway, which provides a novel insight in the mechanism of TINCR and PKCepsilon during MpostC treatment of I/R injury.

References

• Ref 1: LncRNA ACART protects cardiomyocytes from apoptosis by activating PPAR-Gamma/Bcl-2 pathwayJ Cell Mol Med. 2020 Jan;24(1):737-746. doi: 10.1111/jcmm.14781. Epub 2019 Nov 20.
• Ref 2: Morphine post-conditioning-induced up-regulation of lncRNA TINCR protects cardiomyocytes from ischemia-reperfusion injury via inhibiting degradation and ubiquitination of FGF1QJM. 2020 Dec 1;113(12):859-869. doi: 10.1093/qjmed/hcaa088.