Molecule Information

General Information of the Molecule (ID: Mol01982)

Name	Hematopoietic SH2 domain containing (HSH2D) ,Homo sapiens
Synonyms	HSH2D; ALX Click to Show/Hide
Molecule Type	Protein
Gene Name	HSH2D
Gene ID	84941
Location	chr19:16,134,028-16,158,575[+]
Sequence	MTEAGKLPLPLPPRLDWFVHTQMGQLAQDGVPEWFHGAISREDAENLLESQPLGSFLIRV SHSHVGYTLSYKAQSSCCHFMVKLLDDGTFMIPGEKVAHTSLDALVTFHQQKPIEPRREL LTQPCRQKDPANVDYEDLFLYSNAVAEEAACPVSAPEEASPKPVLCHQSKERKPSAEMNR ITTKEATSSCPPKSPLGETRQKLWRSLKMLPERGQRVRQQLKSHLATVNLSSLLDVRRST VISGPGTGKGSQDHSGDPTSGDRGYTDPCVATSLKSPSQPQAPKDRKVPTRKAERSVSCI EVTPGDRSWHQMVVRALSSQESKPEHQGLAEPENDQLPEEYQQPPPFAPGYC Click to Show/Hide
Function	May be a modulator of the apoptotic response through its ability to affect mitochondrial stability (By similarity). Adapter protein involved in tyrosine kinase and CD28 signaling. Seems to affect CD28-mediated activation of the RE/AP element of the interleukin-2 promoter. Click to Show/Hide
Uniprot ID	HSH2D_HUMAN
Ensembl ID	ENSG00000196684
HGNC ID	HGNC:24920
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

UAPP: Unusual Activation of Pro-survival Pathway

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Methotrexate

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Unusual Activation of Pro-survival Pathway (UAPP)			Click to Show/Hide
Disease Class: Acute lymphocytic leukemia				[1]
Resistant Disease	Acute lymphocytic leukemia [ICD-11: 2B33.0]			Disease Info
Resistant Drug	Methotrexate			Drug Info
Molecule Alteration	Expression		Up-regulation
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	HuT-78 cells	Peripheral blood	Homo sapiens (Human)	CVCL_0337
Experiment for Molecule Alteration	Western blotting analysis
Experiment for Drug Resistance	CCK-8 assay
Mechanism Description	The expression of HSH2D was downregulated in T-ALL compared with B-cell ALL. Western blotting and reverse transcription-quantitative PCR demonstrated that the overexpression of HSH2 resulted in the inhibition of CD28-mediated IL-2 activation. In related experiments with drug-resistant cell lines, it was determined that HSH2D expression is necessary for HuT-78 cells to be resistant to MTX.

References

Ref 1	HSH2D contributes to methotrexate resistance in human T cell acute lymphoblastic leukaemia .Oncol Rep. 2020 Nov;44(5):2121-2129. doi: 10.3892/or.2020.7772. Epub 2020 Sep 17. 10.3892/or.2020.7772