Molecule Information
General Information of the Molecule (ID: Mol01607)
Name |
hsa-miR-125a-5p
,Homo sapiens
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Synonyms |
microRNA 125a
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Molecule Type |
Mature miRNA
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Sequence |
UCCCUGAGACCCUUUAACCUGUGA
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Ensembl ID | |||||
HGNC ID | |||||
Mature Accession | |||||
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Type(s) of Resistant Mechanism of This Molecule
EADR: Epigenetic Alteration of DNA, RNA or Protein
Drug Resistance Data Categorized by Drug
Approved Drug(s)
2 drug(s) in total
Cisplatin
Drug Sensitivity Data Categorized by Their Corresponding Mechanisms | ||||
Epigenetic Alteration of DNA, RNA or Protein (EADR) | ||||
Disease Class: Osteosarcoma | [1] | |||
Sensitive Disease | Osteosarcoma [ICD-11: 2B51.0] | |||
Sensitive Drug | Cisplatin | |||
Molecule Alteration | Expression | Up-regulation |
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Experimental Note | Revealed Based on the Cell Line Data | |||
Cell Pathway Regulation | Cell apoptosis | Activation | hsa04210 | |
Cell colony | Inhibition | hsa05200 | ||
Cell proliferation | Inhibition | hsa05200 | ||
In Vitro Model | HEK293T cells | Kidney | Homo sapiens (Human) | CVCL_0063 |
MG63 cells | Bone marrow | Homo sapiens (Human) | CVCL_0426 | |
SAOS-2 cells | Bone marrow | Homo sapiens (Human) | CVCL_0548 | |
U2OS cells | Bone | Homo sapiens (Human) | CVCL_0042 | |
Experiment for Molecule Alteration |
qRT-PCR | |||
Experiment for Drug Resistance |
MTT assay; Flow cytometry assay | |||
Mechanism Description | ANRIL-silenced cells were more sensitive to cisplatin and the expression level of miR-125a-5p was elevated in ANRIL-silenced cells. | |||
Disease Class: Esophageal squamous cell carcinoma | [2] | |||
Sensitive Disease | Esophageal squamous cell carcinoma [ICD-11: 2B70.3] | |||
Sensitive Drug | Cisplatin | |||
Molecule Alteration | Expression | Up-regulation |
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Experimental Note | Revealed Based on the Cell Line Data | |||
Cell Pathway Regulation | Cell apoptosis | Activation | hsa04210 | |
Cell invasion | Inhibition | hsa05200 | ||
Cell migration | Inhibition | hsa04670 | ||
Cell proliferation | Inhibition | hsa05200 | ||
STAT3 signaling pathway | Inhibition | hsa04550 | ||
In Vitro Model | ECA-109 cells | Esophagus | Homo sapiens (Human) | CVCL_6898 |
TE-1 cells | Esophagus | Homo sapiens (Human) | CVCL_1759 | |
EC9706 cells | Esophagus | Homo sapiens (Human) | CVCL_E307 | |
EC1 cells | Esophagus | Homo sapiens (Human) | CVCL_DC74 | |
KYSE70 cells | Esophagus | Homo sapiens (Human) | CVCL_1356 | |
kYSE450 cells | Esophagus | Homo sapiens (Human) | CVCL_1353 | |
Experiment for Molecule Alteration |
RT-qPCR | |||
Experiment for Drug Resistance |
CCK8 assay; Flow cytometry assay; Would healing assay; Invasion assay | |||
Mechanism Description | miR 125a 5p and cisplatin markedly inactivated the STAT3 signaling pathway. |
Imatinib
Drug Resistance Data Categorized by Their Corresponding Mechanisms | ||||
Epigenetic Alteration of DNA, RNA or Protein (EADR) | ||||
Disease Class: Gastrointestinal stromal tumor | [3] | |||
Resistant Disease | Gastrointestinal stromal tumor [ICD-11: 2B5B.0] | |||
Resistant Drug | Imatinib | |||
Molecule Alteration | Expression | Up-regulation |
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Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell invasion | Activation | hsa05200 | |
Cell migration | Activation | hsa04670 | ||
Cell proliferation | Activation | hsa05200 | ||
In Vitro Model | GIST882 cells | Gastric | Homo sapiens (Human) | CVCL_7044 |
GIST48 cells | Gastric | Homo sapiens (Human) | CVCL_7041 | |
Experiment for Molecule Alteration |
RT-qPCR | |||
Experiment for Drug Resistance |
WST-1 assay | |||
Mechanism Description | miR-125a-5p expression modulated imatinib sensitivity in GIST882 cells with a homozygous kIT mutation but not in GIST48 cells with double kIT mutations. Overexpression of miR-125a-5p suppressed PTPN18 expression, and silencing of PTPN18 expression increased cell viability in GIST882 cells upon imatinib treatment. PTPN18 protein levels were significantly lower in the imatinib-resistant GISTs and inversely correlated with miR-125a-5p. Furthermore, several microRNAs were significantly associated with metastasis, kIT mutational status and survival. |
References
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