Molecule Information

General Information of the Molecule (ID: Mol01306)

Name	Novel transcript, antisense to MYRFL (ENST00000547547) ,Homo sapiens
Synonyms	ENST00000547547 Click to Show/Hide
Molecule Type	LncRNA
Gene Name	C13orf29, LINC00346, NCRNA00346
Location	chr12:69946543-69947081[-]
Ensembl ID	ENSG00000257241
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

Click to Show/Hide the Full List of Drugs

Fluorouracil

Click to Show/Hide

Drug Resistance Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Disease Class: Colorectal cancer				[1]
Resistant Disease	Colorectal cancer [ICD-11: 2B91.1]			Disease Info
Resistant Drug	Fluorouracil			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Revealed Based on the Cell Line Data
In Vitro Model	HCT116 cells	Colon	Homo sapiens (Human)	CVCL_0291
	LOVO cells	Colon	Homo sapiens (Human)	CVCL_0399
	HCT116/5-FU cells	Colon	Homo sapiens (Human)	CVCL_AU09
	LOVO/5-FU cells	Colon	Homo sapiens (Human)	CVCL_0399
Experiment for Molecule Alteration	qPCR
Experiment for Drug Resistance	MTT assay; Flow cytometric analysis
Mechanism Description	Knockdown of miR31 increased the 5-FU sensitivity of CRC cells at least partly by upregulation of apoptosis. Overexpression of ENST00000547547 suppressed the anti-apoptotic effect of miR31 via competitive binding to it. ENST00000547547 reduces the 5-FU resistance via competitive binding to miR31 in CRC cells.

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Disease Class: Colorectal cancer				[1]
Sensitive Disease	Colorectal cancer [ICD-11: 2B91.1]			Disease Info
Sensitive Drug	Fluorouracil			Drug Info
Molecule Alteration	Expression		Up-regulation
Experimental Note	Revealed Based on the Cell Line Data
In Vitro Model	HCT116 cells	Colon	Homo sapiens (Human)	CVCL_0291
	LOVO cells	Colon	Homo sapiens (Human)	CVCL_0399
	HCT116/5-FU cells	Colon	Homo sapiens (Human)	CVCL_AU09
	LOVO/5-FU cells	Colon	Homo sapiens (Human)	CVCL_0399
Experiment for Molecule Alteration	qRT-PCR
Experiment for Drug Resistance	MTT assay; Flow cytometric analysis
Mechanism Description	ABCB9 is a transporter which was reported to be targeted by miR31, involved in cisplatin-induced apoptosis, thus knockdown of miR31 increases the 5-FU sensitivity of CRC cell lines. ENST00000547547 reduces the 5-FU resistance via competitive binding to miR31.

References

Ref 1	The long non-coding RNA ENST00000547547 reduces 5-fluorouracil resistance of colorectal cancer cells via competitive binding to microRNA-31. Oncol Rep. 2018 Jan;39(1):217-226. doi: 10.3892/or.2017.6082. Epub 2017 Nov 7.