Molecule Information

General Information of the Molecule (ID: Mol01125)

Name	Tunicamycin resistance protein (TMRB) ,Bacillus subtilis
Synonyms	BSU03140 Click to Show/Hide
Molecule Type	Protein
Gene Name	tmrB
Gene ID	938346
Sequence	MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL RRFADRLMVKLNHIRIK Click to Show/Hide
Function	Involved in the resistance to tunicamycin. Binds ATP. Click to Show/Hide
Uniprot ID	TMRB_BACSU
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Bacillaceae Genus: Bacillus Species: Bacillus subtilis

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Investigative Drug(s)

1 drug(s) in total

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Tunicamycin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)		Click to Show/Hide
Disease Class: Bacillus subtilis infection			[1]
Resistant Disease	Bacillus subtilis infection [ICD-11: 1G40.1]		Disease Info
Resistant Drug	Tunicamycin		Drug Info
Molecule Alteration	Expression	Inherence
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Bacillus subtilis strain		1423
	Escherichia coli strain		562
Experiment for Molecule Alteration	SDS-PAGE assay
Mechanism Description	Overproduction of TmrB protein, a 22.5-kDa protein with an N-terminal ATP-binding region and a C-terminal amphiphilic alpha-helix, confers resistance to tunicamycin on Bacillus subtilis. TmrB protein was found to bind Sepharose 6B to which tunicamycin was covalently linked. Experiments with mutant proteins found that the C-terminal region of TmrB protein might be involved in the binding to tunicamycin.

References

Ref 1	TmrB protein, which confers resistance to tunicamycin on Bacillus subtilis, binds tunicamycin. Biosci Biotechnol Biochem. 1995 Feb;59(2):321-2. doi: 10.1271/bbb.59.321.