Molecule Information

General Information of the Molecule (ID: Mol00978)

Name	Imipenem-hydrolyzing beta-lactamase (NMCA) ,Enterobacter cloacae
Synonyms	Carbapenemase; NMC-A Click to Show/Hide
Molecule Type	Protein
Gene Name	nmcA
Sequence	MSLNVKQSRIAILFSSCLISISFFSQANTKGIDEIKNLETDFNGRIGVYALDTGSGKSFS YRANERFPLCSSFKGFLAAAVLKGSQDNRLNLNQIVNYNTRSLEFHSPITTKYKDNGMSL GDMAAAALQYSDNGATNIILERYIGGPEGMTKFMRSIGDEDFRLDRWELDLNTAIPGDER DTSTPAAVAKSLKTLALGNILSEHEKETYQTWLKGNTTGAARIRASVPSDWVVGDKTGSC GAYGTANDYAVVWPKNRAPLIISVYTTKNEKEAKHEDKVIAEASRIAIDNLK Click to Show/Hide
Function	Hydrolyzes carbapenems such as imipenem, which are extended-spectrum beta-lactam antibiotics. Click to Show/Hide
Uniprot ID	BLAN_ENTCL
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Enterobacter Species: Enterobacter cloacae

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Ampicillin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Enterobacter cloacae infection			[1]
Resistant Disease	Enterobacter cloacae infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Ampicillin		Drug Info
Molecule Alteration	Expression	Inherence
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Escherichia coli strain JM109		83333
	Enterobacter cloacae strain NOR-1		550
Experiment for Molecule Alteration	Dideoxynucleotide chain-termination method assay
Experiment for Drug Resistance	MIC assay
Mechanism Description	Here we report a gene encoding a carbapenemase, which was cloned from the chromosome of a clinical isolate of Enterobacter cloacae, strain NOR-1, into pACYC184 plasmid in Escherichia coli. Unlike all the sequenced carbapenemases, which are class B metallo-beta-lactamases, the mature protein (NmcA) is a class A serine beta-lactamase. NmcA shares the highest amino acid identity (50%) with the extended-spectrum class A beta-lactamase MEN-1 from Escherichia coli.

References

Ref 1	Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7693-7. doi: 10.1073/pnas.91.16.7693.