Molecule Information

General Information of the Molecule (ID: Mol00973)

Name	Glutathione biosynthesis bifunctional protein GshAB (GSHAB ) ,Enterococcus faecalis
Synonyms	Gamma-GCS-GS; GCS-GS; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase; gshF; EF_3089 Click to Show/Hide
Molecule Type	Protein
Gene Name	gshAB
Sequence	MNYRELMQKKNVRPYVLMARFGLEKENQRSTREGLLATTEHPTVFGNRSYHPYIQTDFSE TQLELITPVANSGTEMLRFLDAIHDVARRSIPEDEMLWPLSMPPQLPTKDEEIKIAKLDQ YDAVLYRRYLAKEYGKRKQMVSGIHFNFEYDQALIQQLYDEQSEVTDCKQFKTKVYMKVA RNFLRYRWLITYLFGASPVSEDRYFRVYDDQPQEPVRSIRNSTYGYRNHDNVKVSYASLE RYLEDIHRMVENGLLSEEKEFYAPVRLRGGKQMSDLPKTGIRYIELRNLDLNPFSRLGIV EDTVDFLHYFMLYLLWTDEKEEADEWVKTGDIFNEQVALGHPHETIKLIAEGDRIFSEMI DMLDALGIRKGKEVVGKYYQQLRNPQDTVSGKMWTIIQENSNSELGNIFGNQYQSMAFER PYQLAGFREMELSTQIFLFDAIQKGLEIEILDEQEQFLKLQHGEHIEYVKNANMTSKDNY VVPLIMENKTVTKKILSAAGFHVPGGEEFSSFIEAQEAHLRYANKAFVVKPKSTNYGLGI TIFKEGASLEDFTEALRIAFKEDTAVLIEEFLPGTEYRFFVLDNDVKAIMLRVPANVTGD GKHTVEELVAAKNSDPLRGTNHRAPLELIQLNDLEKLMLKEQGLTIYSVPEKEQIVYLRE NSNVSTGGDSIDMTDVIDDSYKQIAIEAVAALGAKICGIDLIIPDKDVKGTRDSLTYGII EANFNPAMHMHVYPYAGQGRRLTMDVLKLLYPEVVQ Click to Show/Hide
Function	Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine. Click to Show/Hide
Uniprot ID	GSHAB_ENTFA
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Kingdom: N.A. Phylum: Firmicutes Class: Bacilli Order: Lactobacillales Family: Enterococcaceae Genus: Enterococcus Species: Enterococcus faecalis

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Daptomycin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Daptomycin		Drug Info
Molecule Alteration	Missense mutation	p.E354K
Experimental Note	Identified from the Human Clinical Data
In Vitro Model	Enterococcus faecalis S613		699185
Experiment for Molecule Alteration	Whole genome sequence assay; Allelic frequency measurement assay
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	As a glutathione synthase, GshF has previously been implicated in the oxidative stress response across multiple species. GshF is commonly found among mammalian pathogens and could have a role in mitigating DNA damage caused by general oxidative. stress.

References

Ref 1	Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance. Antimicrob Agents Chemother. 2013 Nov;57(11):5373-83. doi: 10.1128/AAC.01473-13. Epub 2013 Aug 19.