Molecule Information

General Information of the Molecule (ID: Mol00880)

Name	Chloroquine resistance transporter (CRT) ,Toxoplasma gondii
Synonyms	CRT Click to Show/Hide
Molecule Type	Protein
Gene Name	CRT
Sequence	MPPAHHGSGGRRRPGRGNKGKRDTEAGMTASPDPGYMRPETHAAPSQQTDVRSPASAREH RNADVGVAAPDALTPNAGEQKEVEGVAKVNVAVSSDQPPDWAPSQSDLPPSLSPTTASRP ATSSRSPRARSRHSPVASSSAFSSPAPSASALTSASGVPEAPLAAPELKHTLAADEGNPE PRLEGPVERLHARQENPLTDSSDGSYILLEEGESQRACLDRKNRHLRQTTPPGVWTTADL ASQNSHSASFASGFRRALLPSGGSGDHDEQASDCRASLRGMQRPCFGSPSTDTRMGAAEG LPLASRRRRQHWRRELRAFGAVALACLRASWHGMKRARLRATRWIDRNAATVRVACYTFL LLVTSTGNTICFKKMIDKMPNYSPCLTQVTTVVFVPVFFALSLYTDYAGGLPQEMADFPK RNFAVMGFLDSFSGVMAIIGAVHTTGTTQVVLQQSCIVFSLLASIVMLRKRFHAAHYLGA LVIILGVLVVKLPDLLHPSSDGGGDVFVFNLLYLLSNLPTAVSCVYKEVAFRGVEMGTNY LQAWVALFQFLIGFLVLPLNALPVLGPQRVPLAELPASLWNGTRCLFGFNTIVTNCGGAG NMESPCDNCEGAWKYVGMYLSFNLLYNMFIIFVVKSGGAALTFLVSTLRLPVTALAFCSR AIMGDRAVPPKATDFYGLLVLILGLVIYRAGGIMKRRAQRRAVAAARGHTSSPMMLTPRE EEQIGTIFVEEVFAAGELEDGGVTEEDETDDDTSEVEVHPVFSSVVASEPPHVYVHTKRH SHSDGGYHKLPACGSSPAAFTPFTQRMPGTGSESCSRRRNRDGDDERSPRSHACSFDEET GFAGGTGTGRHFSSPGTALSPNRVGGYEPPSMHAVQPAVIGKSRANNGCI Click to Show/Hide
Uniprot ID	A0A059XKS9_TOXGO
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Apicomplexa Class: Conoidasida Order: Eucoccidiorida Family: Sarcocystidae Genus: Toxoplasma Species: Toxoplasma gondii

Type(s) of Resistant Mechanism of This Molecule

IDUE: Irregularity in Drug Uptake and Drug Efflux

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Chloroquine

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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Irregularity in Drug Uptake and Drug Efflux (IDUE)			Click to Show/Hide
Disease Class: Malaria				[1], [2]
Sensitive Disease	Malaria [ICD-11: 1F45.0]			Disease Info
Sensitive Drug	Chloroquine			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Revealed Based on the Cell Line Data
Cell Pathway Regulation	Cell proliferation		Inhibition	hsa05200
In Vitro Model	Human foreskin fibroblasts	N.A.	Saccharomyces cerevisiae	N.A.
	Toxoplasma gondii Prudetaku80S/Luc			1080348
In Vivo Model	C57BL/6J mice xenograft model			Mus musculus
Experiment for Molecule Alteration	Western blotting analysis
Experiment for Drug Resistance	Tachyzoite plaque assay
Mechanism Description	Rrecombinant PfCRT transports amino acids, small peptides, and chloroquine, suggests that PfCRT functions to transport products of hemoglobin digestion out of the digestive vacuole. TgCRT is also able to transport amino acids and small peptides out of the VAC. TgCRT-deficient tachyzoites also grow more slowly in vitro and are compromised in their ability to cause mortality in mice during acute infection, suggesting that an inability to transport digested material out of the VAC and into the parasite cytosol has a moderate effect on T. gondii tachyzoites.

References

Ref 1	Characterization of the chloroquine resistance transporter homologue in Toxoplasma gondii. Eukaryot Cell. 2014 Nov;13(11):1360-70. doi: 10.1128/EC.00027-14. Epub 2014 May 23.
Ref 2	Role of Toxoplasma gondii Chloroquine Resistance Transporter in Bradyzoite Viability and Digestive Vacuole Maintenance. mBio. 2019 Aug 6;10(4):e01324-19. doi: 10.1128/mBio.01324-19.