Molecule Information

General Information of the Molecule (ID: Mol00790)

Name	Aminoglycoside 6-adenylyltransferase (A6AD) ,Bacillus subtilis
Synonyms	6-O-adenyl-transferase; AAD(6); ANT(6); Aminoglycoside inactivating enzyme; Sm inactivating enzyme; Streptomycin 6-adenylyltransferase; BSU26790; HIR78_15755 Click to Show/Hide
Molecule Type	Protein
Gene Name	aadK
Gene ID	938061
Sequence	MRSEQEMMDIFLDFALNDERIRLVTLEGSRTNRNIPPDNFQDYDISYFVTDVESFKENDQ WLEIFGKRIMMQKPEDMELFPPELGNWFSYIILFEDGNKLDLTLIPIREAEDYFANNDGL VKVLLDKDSFINYKVTPNDRQYWIKRPTAREFDDCCNEFWMVSTYVVKGLARNEILFAID HLNEIVRPNLLRMMAWHIASQKGYSFSMGKNYKFMKRYLSNKEWEELMSTYSVNGYQEMW KSLFTCYALFRKYSKAVSEGLAYKYPDYDEGITKYTEGIYCSVK Click to Show/Hide
Function	Mediates bacterial resistance to streptomycin. Adenylates streptomycin on the O-6 residue. Adenylates streptidine on the O-6 residue. Does not act on spectinomycin, neomycin-B or kanamycin (Ref.5984036). Specific for ATP and GTP nucleotides incorporating a purine ring. No reaction with CTP or UTP. Click to Show/Hide
Uniprot ID	AADK_BACSU
*Click to Show/Hide the Complete Species Lineage*
Kingdom: N.A. Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Bacillaceae Genus: Bacillus Species: Bacillus subtilis

Type(s) of Resistant Mechanism of This Molecule

DISM: Drug Inactivation by Structure Modification

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Streptomycin

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Drug Resistance Data Categorized by Their Corresponding Mechanisms
Drug Inactivation by Structure Modification (DISM)		Click to Show/Hide
Disease Class: Bacterial infection			[1], [2]
Resistant Disease	Bacterial infection [ICD-11: 1A00-1C4Z]		Disease Info
Resistant Drug	Streptomycin		Drug Info
Molecule Alteration	Expression	Up-regulation
Experimental Note	Discovered Using In-vivo Testing Model
In Vitro Model	Bacillus subtilis strain 168		1423
	Bacillus subtilis strain 169		1423
	Bacillus subtilis strain 170		1423
	Bacillus subtilis strain 171		1423
	Bacillus subtilis strain 172		1423
	Bacillus subtilis strain 173		1423
Experiment for Drug Resistance	Agar dilution method assay
Mechanism Description	B. subtilis 168 produce s a chromosomally encoded aminoglycoside 6-adenylyltransferase, AAD(6),which inactivates S M by adenylation at the C-6 position of streptomycin.

References

Ref 1	Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenylyltransferase from Bacillus subtilis Marburg 168. Gene. 1989 May 30;78(2):377-8. doi: 10.1016/0378-1119(89)90241-2.
Ref 2	Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase. FEMS Microbiol Lett. 1993 Nov 15;114(1):47-52. doi: 10.1016/0378-1097(93)90140-w.