Molecule Information

General Information of the Molecule (ID: Mol00708)

Name	WT1 antisense RNA (WT1-AS) ,Homo sapiens
Synonyms	WIT-1; WIT11; WT1AS; WT1-AS Click to Show/Hide
Molecule Type	LncRNA
Gene Name	WT1-AS
Gene ID	51352
Location	chr11:32,435,518-32,500,632[+]
Ensembl ID	ENSG00000183242
HGNC ID	HGNC:18135
*Click to Show/Hide the Complete Species Lineage*
Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens

Type(s) of Resistant Mechanism of This Molecule

EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Drug

Approved Drug(s)

1 drug(s) in total

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Doxorubicin

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Drug Sensitivity Data Categorized by Their Corresponding Mechanisms
Epigenetic Alteration of DNA, RNA or Protein (EADR)			Click to Show/Hide
Disease Class: Hepatocellular carcinoma				[1]
Sensitive Disease	Hepatocellular carcinoma [ICD-11: 2C12.2]			Disease Info
Sensitive Drug	Doxorubicin			Drug Info
Molecule Alteration	Expression		Down-regulation
Experimental Note	Revealed Based on the Cell Line Data
Cell Pathway Regulation	Cell apoptosis		Activation	hsa04210
	Cell proliferation		Inhibition	hsa05200
	JAKT2/STAT3/MAPK signaling pathway		Inhibition	hsa04659
In Vitro Model	HepG2 cells	Liver	Homo sapiens (Human)	CVCL_0027
	L02 cells	Liver	Homo sapiens (Human)	CVCL_6926
Experiment for Molecule Alteration	Western blot analysis
Experiment for Drug Resistance	EDU assay; Flow cytometry assay
Mechanism Description	WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1.WT1-AS expression correlated negatively with WT1 expression in HCC tumor tissue. kaplan-Meier curve analysis revealed that WT1-AS expression is a reliable indicator of HCC prognosis. The downregulation of WT1 expression by WT1-AS promoted cell apoptosis by suppressing the JAk/STAT3 signaling pathway. Bioinformatics analysis showed that WT1-AS downregulates WT1 by binding to the TATA region of the WT1 promotor. WT1-AS was also able to reverse WT1-mediated resistance to Dox based chemotherapy in HCC cells.

Disease- and Tissue-specific Abundances of This Molecule

ICD Disease Classification 02

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Liver cancer [ICD-11: 2C12]

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Differential expression of molecule in resistant diseases
The Studied Tissue	Liver
The Specified Disease	Liver cancer
The Expression Level of Disease Section Compare with the Healthy Individual Tissue	p-value: 1.36E-02; Fold-change: -1.91E-01; Z-score: -4.17E-01
The Expression Level of Disease Section Compare with the Adjacent Tissue	p-value: 9.59E-09; Fold-change: -2.58E-01; Z-score: -8.28E-01
The Expression Level of Disease Section Compare with the Other Disease Section	p-value: 6.44E-01; Fold-change: -4.41E-02; Z-score: -2.58E-01
Molecule expression in the normal tissue adjacent to the diseased tissue of patients Molecule expression in the diseased tissue of patients Molecule expression in the normal tissue of healthy individuals Molecule expression in tissue other than the diseased tissue of patients
Disease-specific Molecule Abundances		Click to View the Clearer Original Diagram
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Tissue-specific Molecule Abundances in Healthy Individuals

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References

Ref 1	WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1. J Exp Clin Cancer Res. 2015 Oct 13;34:119. doi: 10.1186/s13046-015-0233-7.